IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Characterization and biological effects of extracts from winery by-products

Characterization and biological effects of extracts from winery by-products

Abstract

Pomace, stem, grapevine leaves, and vine shoots arise as so called winery by-products during the wine production process. Due to their high content of secondary plant metabolites, such as polyphenols, their usage as sources of bioactive compounds offers an opportunity to obtain value-added products for the food and pharmaceutical industry. The aim of the present study was to investigate extracts from winery by-products of Vitis vinifera L. cv. Riesling from the region ‚Pfalz‘ in Rhineland-Platinate, Germany with regard to their chemical composition and biological effect in vitro. Total phenolic contents (TPC) of pomace, stem, vine leaf, and vine shoot extracts were determined by Folin-Ciocalteu method and polyphenolic profiles were characterized by HPLC-UV/Vis-ESI-MS/MS. The extracts showed TPCs ranging from 432 to 665 mg GAE/g extract. Besides flavanols, as for example catechin, epicatechin and procyanidins, phenolic acids and flavonols, such as quercetin und kaempferol derivates were tentatively identified, amongst others, by HPLC-UV/Vis-ESI-MS/MS analysis in the negative ion mode. Stilbenes represent an additional group of polyphenols present in the extracts from winery by-products, including trans-resveratrol, piceid, piceatannol and ε-viniferin being identified. In the human hepatocarcinoma cell line HepG2 effects of the extracts on cell viability, intracellular ATP, the mitochondrial membrane potential (MMP), and tert-butyl hydroperoxide (TBH)-induced intracellular reactive oxygen species (ROS) were determined in vitro. Dose-dependent cytotoxic effects were observed besides protective effects regarding TBH-induced intracellular ROS level, and partially impaired MMP. Thus, winery by-products represent interesting sources of bioactive compounds exerting positive and/or negative effects on mitochondrial function in liver cells.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Fuchs Christine1, Bakuradze Tamara1, Stegmüller Simone1, Steinke Regina1 and Richling Elke1

1TU Kaiserslautern, Department of Chemistry, Division of Food Chemistry

Contact the author

Keywords

polyphenols, HPLC-UV/Vis-ESI-MS/MS, extracts of winery by-products, Vitis vinifera L. cf. Riesling, liver cells

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.

Aroma profile evaluation in whole grape juices

Table grapes (Vitis labrusca and hybrids) are widely cultivated in Brazil [1] due to the climate, their resistance to disease and the way they are consumed and commercialized, either in-natura or for processing, producing whole juices, jams and table wines.

A worldwide perspective on viticultural zoning

Cet article répertorie les intérêts et problèmes du zonage viticole dans une perspective mondiale. Le zonage est un besoin pour chacun des vignobles mondiaux où il correspond à des applications, définitions et approches variées. Les objectifs du zonage changent de concert avec les besoins du marché mondial du vin, qui ne cesse de croître.

GrapeBreed4IPM: developing sustainable solutions for viticulture through multi-actor innovation targeting breeding for integrated pest management

According to the World Economic Forum and the European Union’s Biodiversity Strategy for 2030, the loss of biodiversity and the collapse of ecosystems are major threats facing humanity in the future.

Improved vineyard sampling efficiency using aerial NDVI

Random sampling is often considered to be the best protocol for fruit sampling because it is assumed to produce a sample that best represents the vineyard population.