IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Characterization and biological effects of extracts from winery by-products

Characterization and biological effects of extracts from winery by-products

Abstract

Pomace, stem, grapevine leaves, and vine shoots arise as so called winery by-products during the wine production process. Due to their high content of secondary plant metabolites, such as polyphenols, their usage as sources of bioactive compounds offers an opportunity to obtain value-added products for the food and pharmaceutical industry. The aim of the present study was to investigate extracts from winery by-products of Vitis vinifera L. cv. Riesling from the region ‚Pfalz‘ in Rhineland-Platinate, Germany with regard to their chemical composition and biological effect in vitro. Total phenolic contents (TPC) of pomace, stem, vine leaf, and vine shoot extracts were determined by Folin-Ciocalteu method and polyphenolic profiles were characterized by HPLC-UV/Vis-ESI-MS/MS. The extracts showed TPCs ranging from 432 to 665 mg GAE/g extract. Besides flavanols, as for example catechin, epicatechin and procyanidins, phenolic acids and flavonols, such as quercetin und kaempferol derivates were tentatively identified, amongst others, by HPLC-UV/Vis-ESI-MS/MS analysis in the negative ion mode. Stilbenes represent an additional group of polyphenols present in the extracts from winery by-products, including trans-resveratrol, piceid, piceatannol and ε-viniferin being identified. In the human hepatocarcinoma cell line HepG2 effects of the extracts on cell viability, intracellular ATP, the mitochondrial membrane potential (MMP), and tert-butyl hydroperoxide (TBH)-induced intracellular reactive oxygen species (ROS) were determined in vitro. Dose-dependent cytotoxic effects were observed besides protective effects regarding TBH-induced intracellular ROS level, and partially impaired MMP. Thus, winery by-products represent interesting sources of bioactive compounds exerting positive and/or negative effects on mitochondrial function in liver cells.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Fuchs Christine1, Bakuradze Tamara1, Stegmüller Simone1, Steinke Regina1 and Richling Elke1

1TU Kaiserslautern, Department of Chemistry, Division of Food Chemistry

Contact the author

Keywords

polyphenols, HPLC-UV/Vis-ESI-MS/MS, extracts of winery by-products, Vitis vinifera L. cf. Riesling, liver cells

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Bio-protection by one strain of M. Pulcherrima: microbiological and chemical impacts in red wines

In oenology, bio-protection consists in adding bacteria, yeasts or a mixture of microorganisms on grape must before fermentation in order to reduce the use of chemical compounds such as sulphites.

Could intermittent shading, as produced in agrivoltaics, mitigate global warming effects on grapevine?

Global warning increases evaporative demand and accelerates grapevine phenology. As a consequence, the ripening phase shifts to warmer and drier periods. This results in lower acidity and higher sugar levels in berries, yielding too alcoholic wines with altered organoleptic properties. Agrivoltaics, which combines crop and renewable energy production on the same land using photovoltaic panels, emerged as a promising innovation to counteract these impacts by partially shading the plants.

Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

The Maillard reaction (MR) is a non-enzymatic reaction between reducing sugars and amino acids, resulting in the production of volatile and flavour-active compounds.

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.

Effect of the plant sink/source balance on the chemical content of red table grapes (Vitis vinifera L.).

PPhloem transport of assimilates provides the materials needed for the growth and development of reproductive structures, storage and developing organs, and has long been recognized as a major determinant in crop yield.