IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Abstract

Among the macromolecules of enological interest in white wines, much attention has been paid to polysaccharides. They have their origin in grape skin and pulp cell walls and includes polysaccharides rich in arabinose and galactose (PRAG), rhamnogalacturonans type II (RG-II) and homogalacturonans (HG); and cell walls from yeasts, constituted by mannoproteins (MP) and glucans. Polysaccharide content and composition change during fermentation and bottle aging.
Polysaccharide composition of white wines will depend, among other factors, on the pre-fermentative treatment applied to the grapes. Direct pressing or pre-fermentation maceration of crushed and destemmed grapes are usually applied. There are few studies analyzing the effect these treatments on the content of polysaccharides. Recently, the International Organization of Vine and Wine has approved the use of sonication of crushed grapes to promote the extraction of their compounds. However, there are no studies on the effect of sonication on crushed and white grapes on the content of polysaccharides in the musts and during the aging in bottle.
The aim of this study was to study the effect of direct pressing of white grapes, prefermentative maceration and prefermentative sonication of crushed and destemmed grapes on the polysaccharide composition in musts from Vitis vinifera L. cv. Airén. It was also studied the effect during the bottle aging.
Airén white grapes were destemmed and crushed. One batch was directly pressed into a pneumatic press; other was pressed after 4 hours of-fermentative maceration; and other was treated with power ultrasound at 30 kHz before pressing. Samples were taken of the raked musts, when bottling and after 6 months of bottle aging. Glycosyl residue composition was analyzed as previously described (Guadalupe et al., 2012; Ayestarán et al., 2004).
All the musts showed contents of glucosyl monosaccharides significantly higher than pectic monosaccharides. Sonication treatment improved the release of RG-II and mannans in musts and the pre-fermentative maceration favored the extraction of PRAG. Musts from direct pressing had the lowest content of total monosaccharides and RG-II. From the must to the time of bottling, there was an increase in the content of RG-II and MP and a decrease of PRAG in all wines evaluated. At the time of bottling, wines from sonicated grapes showed the highest content of pectic monosaccharides and RG-II, while wines from pre-fermentative maceration showed the highest content of glucosyl monosaccharides. After 6 months of bottle aging, all wines showed a decrease in the content of MP and PRAG. Wines made with pre-fermentative maceration showed the highest content of total polysaccharides families, PRAG and MP.
This research was funded by the Ministerio de Ciencia, Innovación y Universidades and Feder Funds, grant number RTI2018-093869-B-C21.

References

Ayestarán, B.; Guadalupe, Z.; León, D. Quantification of major grape polysaccharides (Tempranillo v.) released by maceration enzymes during the fermentation process. Anal. Chim. Acta. 2004, 513, 29–39.
Guadalupe, Z.; Martínez-Pinilla, O.; Garrido, A.; Carrillo, J. D.; Ayestarán, B. Quantitative determination of wine polysaccharides by gas chromatography–mass spectrometry (GC–MS) and size exclusion chromatography (SEC). Food Chem. 2012, 131, 367–374.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Canalejo Diego1, Zhao Feng1, Martínez-Lapuente Leticia1, Guadalupe Zenaida1, Ayestarán Belén1, Pérez-Porras Paula2, Bautista-Ortín Ana Belén2 and Gómez-Plaza Encarna2

1Institute of Vine and Wine Sciences, ICVV (University of La Rioja, Government of La Rioja and CSIC)
2Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia

Contact the author

Keywords

White wine; Monosaccharides; High-power ultrasounds; Direct pressing; Pre-fermentation maceration

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.