IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Abstract

Among the macromolecules of enological interest in white wines, much attention has been paid to polysaccharides. They have their origin in grape skin and pulp cell walls and includes polysaccharides rich in arabinose and galactose (PRAG), rhamnogalacturonans type II (RG-II) and homogalacturonans (HG); and cell walls from yeasts, constituted by mannoproteins (MP) and glucans. Polysaccharide content and composition change during fermentation and bottle aging.
Polysaccharide composition of white wines will depend, among other factors, on the pre-fermentative treatment applied to the grapes. Direct pressing or pre-fermentation maceration of crushed and destemmed grapes are usually applied. There are few studies analyzing the effect these treatments on the content of polysaccharides. Recently, the International Organization of Vine and Wine has approved the use of sonication of crushed grapes to promote the extraction of their compounds. However, there are no studies on the effect of sonication on crushed and white grapes on the content of polysaccharides in the musts and during the aging in bottle.
The aim of this study was to study the effect of direct pressing of white grapes, prefermentative maceration and prefermentative sonication of crushed and destemmed grapes on the polysaccharide composition in musts from Vitis vinifera L. cv. Airén. It was also studied the effect during the bottle aging.
Airén white grapes were destemmed and crushed. One batch was directly pressed into a pneumatic press; other was pressed after 4 hours of-fermentative maceration; and other was treated with power ultrasound at 30 kHz before pressing. Samples were taken of the raked musts, when bottling and after 6 months of bottle aging. Glycosyl residue composition was analyzed as previously described (Guadalupe et al., 2012; Ayestarán et al., 2004).
All the musts showed contents of glucosyl monosaccharides significantly higher than pectic monosaccharides. Sonication treatment improved the release of RG-II and mannans in musts and the pre-fermentative maceration favored the extraction of PRAG. Musts from direct pressing had the lowest content of total monosaccharides and RG-II. From the must to the time of bottling, there was an increase in the content of RG-II and MP and a decrease of PRAG in all wines evaluated. At the time of bottling, wines from sonicated grapes showed the highest content of pectic monosaccharides and RG-II, while wines from pre-fermentative maceration showed the highest content of glucosyl monosaccharides. After 6 months of bottle aging, all wines showed a decrease in the content of MP and PRAG. Wines made with pre-fermentative maceration showed the highest content of total polysaccharides families, PRAG and MP.
This research was funded by the Ministerio de Ciencia, Innovación y Universidades and Feder Funds, grant number RTI2018-093869-B-C21.

References

Ayestarán, B.; Guadalupe, Z.; León, D. Quantification of major grape polysaccharides (Tempranillo v.) released by maceration enzymes during the fermentation process. Anal. Chim. Acta. 2004, 513, 29–39.
Guadalupe, Z.; Martínez-Pinilla, O.; Garrido, A.; Carrillo, J. D.; Ayestarán, B. Quantitative determination of wine polysaccharides by gas chromatography–mass spectrometry (GC–MS) and size exclusion chromatography (SEC). Food Chem. 2012, 131, 367–374.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Canalejo Diego1, Zhao Feng1, Martínez-Lapuente Leticia1, Guadalupe Zenaida1, Ayestarán Belén1, Pérez-Porras Paula2, Bautista-Ortín Ana Belén2 and Gómez-Plaza Encarna2

1Institute of Vine and Wine Sciences, ICVV (University of La Rioja, Government of La Rioja and CSIC)
2Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia

Contact the author

Keywords

White wine; Monosaccharides; High-power ultrasounds; Direct pressing; Pre-fermentation maceration

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Grapevine yield estimation in a context of climate change: the GraY model

Grapevine yield is a key indicator to assess the impacts of climate change and the relevance of adaptation strategies in a vineyard landscape. At this scale, a yield model should use a number of parameters and input data in relation to the information available and be able to reproduce vineyard management decisions (e.g. soil and canopy management, irrigation). In this study, we used data from six experimental sites in Southern France (cv. Syrah) to calibrate a model of grapevine yield limited by water constraint (GraY). Each yield component (bud fertility, number of berries per bunch, berry weight) was calculated as a function of the soil water availability simulated by the WaLIS water balance model at critical phenological phases. The model was then evaluated in 10 grapegrowers’ plots, covering a diversity of biophysical and technical contexts (soil type, canopy size, irrigation, cover crop). We identified three critical periods for yield formation: after flowering on the previous year for the number of bunches and berries, around pre-veraison and post-veraison of the same year for mean berry weight. Yields were simulated with a model efficiency (EF) of 0.62 (NRMSE = 0.28). Bud fertility and number of berries per bunch were more accurately simulated (EF = 0.90 and 0.77, NRMSE = 0.06 and 0.10, respectively) than berry weight (EF = -0.31, NRMSE = 0.17). Model efficiency on the on-farm plots reached 0.71 (NRMSE = 0.37) simulating yields from 1 to 8 kg/plant. The GraY model is an original model estimating grapevine yield evolution on the basis of water availability under future climatic conditions.  It allows to evaluate the effects of various adaptation levers such as planting density, cover crop management, fruit/leaf ratio, shading and irrigation, in various production contexts.