IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Effect of pre-fermentative strategies on the polysaccharide composition of must and white wines

Abstract

Among the macromolecules of enological interest in white wines, much attention has been paid to polysaccharides. They have their origin in grape skin and pulp cell walls and includes polysaccharides rich in arabinose and galactose (PRAG), rhamnogalacturonans type II (RG-II) and homogalacturonans (HG); and cell walls from yeasts, constituted by mannoproteins (MP) and glucans. Polysaccharide content and composition change during fermentation and bottle aging.
Polysaccharide composition of white wines will depend, among other factors, on the pre-fermentative treatment applied to the grapes. Direct pressing or pre-fermentation maceration of crushed and destemmed grapes are usually applied. There are few studies analyzing the effect these treatments on the content of polysaccharides. Recently, the International Organization of Vine and Wine has approved the use of sonication of crushed grapes to promote the extraction of their compounds. However, there are no studies on the effect of sonication on crushed and white grapes on the content of polysaccharides in the musts and during the aging in bottle.
The aim of this study was to study the effect of direct pressing of white grapes, prefermentative maceration and prefermentative sonication of crushed and destemmed grapes on the polysaccharide composition in musts from Vitis vinifera L. cv. Airén. It was also studied the effect during the bottle aging.
Airén white grapes were destemmed and crushed. One batch was directly pressed into a pneumatic press; other was pressed after 4 hours of-fermentative maceration; and other was treated with power ultrasound at 30 kHz before pressing. Samples were taken of the raked musts, when bottling and after 6 months of bottle aging. Glycosyl residue composition was analyzed as previously described (Guadalupe et al., 2012; Ayestarán et al., 2004).
All the musts showed contents of glucosyl monosaccharides significantly higher than pectic monosaccharides. Sonication treatment improved the release of RG-II and mannans in musts and the pre-fermentative maceration favored the extraction of PRAG. Musts from direct pressing had the lowest content of total monosaccharides and RG-II. From the must to the time of bottling, there was an increase in the content of RG-II and MP and a decrease of PRAG in all wines evaluated. At the time of bottling, wines from sonicated grapes showed the highest content of pectic monosaccharides and RG-II, while wines from pre-fermentative maceration showed the highest content of glucosyl monosaccharides. After 6 months of bottle aging, all wines showed a decrease in the content of MP and PRAG. Wines made with pre-fermentative maceration showed the highest content of total polysaccharides families, PRAG and MP.
This research was funded by the Ministerio de Ciencia, Innovación y Universidades and Feder Funds, grant number RTI2018-093869-B-C21.

References

Ayestarán, B.; Guadalupe, Z.; León, D. Quantification of major grape polysaccharides (Tempranillo v.) released by maceration enzymes during the fermentation process. Anal. Chim. Acta. 2004, 513, 29–39.
Guadalupe, Z.; Martínez-Pinilla, O.; Garrido, A.; Carrillo, J. D.; Ayestarán, B. Quantitative determination of wine polysaccharides by gas chromatography–mass spectrometry (GC–MS) and size exclusion chromatography (SEC). Food Chem. 2012, 131, 367–374.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Canalejo Diego1, Zhao Feng1, Martínez-Lapuente Leticia1, Guadalupe Zenaida1, Ayestarán Belén1, Pérez-Porras Paula2, Bautista-Ortín Ana Belén2 and Gómez-Plaza Encarna2

1Institute of Vine and Wine Sciences, ICVV (University of La Rioja, Government of La Rioja and CSIC)
2Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia

Contact the author

Keywords

White wine; Monosaccharides; High-power ultrasounds; Direct pressing; Pre-fermentation maceration

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Polyphenolic profile and dietary fiber content of skins and seeds from unfermented and fermented grape pomace

The valorization of winemaking byproducts is subordinated to the knowledge of their chemical characteristics. This work concerned the determination of the polyphenolic profile and the dietary fiber content of skins and seeds from unfermented and fermented pomace of different cultivars (Moscato bianco, Cortese, Arneis, Pinot Noir, Barbera, Grignolino, Nebbiolo), sampled from some wineries in the Piedmont area (Italy) during the 2020 harvest.

Organic mulches improve vine vigour, yield and physiological response in a semi-arid region

Recycled organic mulch within the row in vineyard floor management has become an interesting ecological strategy to adapt the crop to climate change consequences in semi-arid regions.
This study aimed to assess the impact of three recycled organic mulches [straw (STR), grape pruning debris (GPD), and spent mushroom compost (SMC)] and two conventional soil management practices [herbicide (HERB) and under-row tillage (TILL)] on vegetative vigour (NDVI), production (kg/plant), and physiological parameters (δ13C in grapes and leaf gas exchange during four grapevine phenology stages). Additionally, temperature and water soil parameters were collected at three soil depths. Data was collected during the 2021 and 2022 grapevine growing seasons in La Rioja, Spain.

Elucidating vineyard site contributions to key sensory molecules: Identification of correlations between elemental composition and volatile aroma profile of site-specific Pinot noir wines

The reproducibility of elemental profile in wines produced across multiple vintages has been previously reported using grapes from a single scion clone of Vitis vinifera L. cv. Pinot noir. The grapevines were grown on fourteen different vineyard sites, from Oregon to southern California in the U.S.A., which span distances from approximately hundreds of meters to 1450 km, while elevations range from near sea level to nearly 500 m. In addition, sensorial (i.e. aroma, taste, and mouthfeel) and chemical (i.e. polyphenolic and volatile) differences across the different vineyard sites have also been observed among these wines at two aging time points. While strong evidence exists to support that grapes grown in different regions can produce wines with unique chemical and sensorial profiles, even when a single clone is used, the understanding of growing site characteristics that result in this reproducible differentiation continues to emerge. One hypothesis is that the elemental profile that a vineyard site imparts to the grape berries and the resulting wine is an important contributor to this differentiation in chemistry and sensory of wines. For example, various classes of enzymes that catalyze the formation of key aroma compounds or their precursors require specific metals. In this work, we begin to report correlations between elemental and volatile aroma profiles of site-specific Pinot noir wines, made under standardized winemaking conditions, that have been previously shown to be distinguished separately by these chemical analyses.

Valutazione comparativa di cloni di Pinot nero per la produzione di vini base spumante in alcuni ambienti del Piemonte

Un vasto programma si riferisce alla verifica di 28 selezioni clonali di Pinot nero atte a vini base spumante. Gli impianti sono stati realizzati in diversi ambienti delle Langhe e del Monferrato nel periodo 1992-1996, in 57 vigneti diversi e su una superficie complessiva di circa 50 Ha.

Apoplastic pH influences Vitis vinifera Barbera recovery responses to short and prolonged drought 

Alteration of sap pH is one of the first chemical changes that occurs within the xylem vessels of plants exposed to drought. Xylem sap acidification accompanied by the accumulation of soluble sugars has been recently documented in several species (Sharp and Davis, 2009; Secchi and Zwieniecki, 2016). Here, Vitis vinifera plants of the anysohydric cultivar Barbera were exposed to either short (no irrigation; SD) or to prolonged drought (continual reduction of 10% water; PD). When comparable severe stress was reached, the potted grapes were re-watered. SD was characterized by fast (2–3 days) stomatal closure and high abscisic acid (ABA) accumulation in xylem sap (>400 μg L−1) and in leaf. In PD plants, the rise in ABA levels was considerably diminished.