IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Molecular binding mechanisms between grape seed polypeptides and wine anthocyanins by fluorescence spectroscopy and computational techniques

Molecular binding mechanisms between grape seed polypeptides and wine anthocyanins by fluorescence spectroscopy and computational techniques

Abstract

In recent years, proteins endogenous to grape have become of great interest to the wine industry because they represent a new alternative to other biopolymers subjected to more legal restrictions (i.e. animal origin and synthetics) that can be used in technological applications to modulate sensory attributes such as wine color and have a positive impact on wine quality.
Due to their great techno-functional value, some major protein components of grape seed endosperm have been identified and its potential use for color protection purposes have been already tested in wines at experimental scale. Notwithstanding, the capability of proteins to modulate sensory properties is highly dependent on their structural features, their ability to form specific 3D conformations, and the interaction with other molecules present in food matrices such as anthocyanin pigments in wines. Recently, we have confirmed by means of theoretical studies that grape seed  globulins can stablish different types of interactions (hydrogen bonding, alkyl and π-π) with the main grape anthocyanin (malvidin 3-gl).To date, however, such as interactions has been scarcely studied. Although a basic molecular interaction mechanism similar to copigmentation has been suggested, many questions still remain unclarified. In this sense, a deeper molecular understanding of the grape seed protein-anthocyanin interactions is needed to better define their potential uses and technological applications.
Thus, the main objective of this work was to explore new molecular binding mechanisms between grape seed polypeptides and wine anthocyanins in model wine solutions through fluorescence spectroscopy and computational techniques. For this purpose, fluorescence extinction spectroscopy was used to experimentally confirm the possible interaction mechanisms, the affinity constants, the stoichiometry of the complexes and other thermodynamic parameters. In addition, molecular modelling techniques, such as docking studies, were used to model the  most energetically favorable binding sites of wine anthocyanins on the grape seed polypeptide. Results will provide insights and substantial information about protection to colors expressed by anthocyanins in red wine.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Chamizo-González Francisco1, Gordillo Arrobas Belén1, Hereida Francisco J.1, Días Ricardo2 and Freitas Víctor2

1Food Colour and Quality Laboratory, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain
2Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto  

Contact the author

Keywords

wine anthocyanin, grape seed polypeptides, molecular interaction, Quenching of fluorescence, computational techniques

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Leaf necrosis induced by the insecticide carbaryl in Vitis rupestris ‘B38’

Carbaryl is an acetylcholine esterase inhibitor-type insecticide used for pest control on grapevine. We repeatedly observed the occurrence of interveinal leaf necrosis following carbaryl spray application in a Vitis rupestris x Vitis riparia F1 hybrid progeny vineyard. Spray applications induced necrosis in this progeny under both Missouri and New York field conditions an approximate one-to-one sensitive-to-insensitive segregation ratio and with 42% concordance. Results of subsequent in vitro experiments established causality between carbaryl treatment and leaf necrosis and confirmed the pattern of segregation observed in the field. We consistently map this phenotype to a major QTL on chromosome 16 of the female parent V. rupestris ‘B38’ regardless of whether we used field or in vitro-generated phenotype data.

Characterization of four Chenin Blanc-rootstock combinations to assess grapevine adaptability to water constraint

Climate change impacts water availability for agriculture, notably in semi-arid regions like South Africa, necessitating research on cultivar and rootstock adaptability to water constraints. To evaluate the performance (vegetative and reproductive) of different Chenin Blanc-rootstock combinations to the two water regimes, a field experiment was established in a model vineyard at Stellenbosch University, South Africa. Chenin Blanc vines grafted onto four different rootstocks (110Richter, 99Richter, 1103Paulsen and US 8-7) were planted in 2020. The vines are managed under two contrasting water conditions – dryland and irrigated (industry norm).

Gas chromatography-olfactometry characterization of corvina and corvinone young and aged wines

AIM AND METHODS: Corvina and Corvinone are the two main grape varieties used in the production of Valpolicella, Recioto and Amarone, top-quality red wines in north-eastern Italy. This work aimed at determining the aroma composition of Corvina and Corvinone experimental wines and identify the main aroma compounds contributing to the aroma characteristics of Corvina and Corvinone monovarietal wines. Five Corvina and five Corvinone wines were studied, the grapes coming from five different vineyards in Valpolicella. Volatile compounds were extracted by SPE and identified and quantified by gas chromatography-mass spectrometry (GC-MS), whereas their aroma impact was determined by gas chromatography- olfactometry (GC-O).RESULTS: Based on the GC-MS-O analysis, 95 odor zones were detected, from which 68 compounds were successfully identified. Using the criterion of a value higher than 30% in modified frequency (MF %), 51 compounds were selected and grouped according to odor similarity. Compounds with values below 30% were discarded.

Genomics and phenomics of root system architecture in grapevine

Adapting viticulture to climate change is crucial, as it presents significant challenges for future grape production.

Genetic causes of SO2 consumption in Saccharomyces cerevisiae

SO2 is used during winemaking for its anti-oxidative and anti-microbial properties. A high SO2 concentration in the wine has negative impacts by hiding wine aromas and delaying malolactic fermentation.