IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Peptides diversity and oxidative sensitivity: case of specific optimized inactivated yeasts

Peptides diversity and oxidative sensitivity: case of specific optimized inactivated yeasts

Abstract

Estimation of the resistance of a wine against oxidation is of great importance for the wine. To that purpose, most of the commonly used chemical assays that are dedicated to estimate the antioxidant (or antiradical) capacity of a wine consist in measuring the capacity of the wine to reduce an oxidative compound or a stable radical. In the must/wine matrix, polyphenols are major compounds likely to react with oxidant or radical, but such reaction generate quinones that then are involved in varietal aroma loss via nucleophilic addition reaction. It raises the paradox that a good antioxidant capacity does not imply a good protection of such sensitive compounds as aromatic compounds which are wine key quality markers.

The authors have developed a methodology focusing on the survival time of a sensitive compound to estimate the oxidative sensitivity of a solution. A labeled nucleophile is monitored by UHPLC-ESI-Q-ToF MS periodically for hours (from 0.5h to 72h) after a chemical initiation of oxidation in wine model solution containing 4-methylcatechol. 7 Cystein containing peptides (alone or in combination) are used to artificially increase the nucleophilic environment (and thus the competition for quinone nucleophilic addition) and estimate the half-life of the labeled nucleophile. In addition, soluble fraction released by different inactivated yeasts are also used as complex source of nucleophiles.

Half-life of the labeled compound is the final expression of several complex mechanisms such as nucleophilic competition, but also oxygen consumption, or catechol reduction.
Independently of the mechanisms, we can observe that increasing the nucleophilic environment improve the half life of the labeled nucleophile. It is notably visible for the specific optimized inactivated yeast which released many cysteine-containing peptides.
Finally, this method relies on the fate of one sensitive nucleophile to estimate the sensitiveness of the whole matrix to oxidation. It estimates the half-life of this compound which allows to compare oxidative sensitivity of different matrices under specific oxidation conditions.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Bahut Florian1, Sieczkowski Nathalie1, Nikolantonaki Maria1 and Gougeon Régis D.1

1Univ. Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, Lallemand SAS, Institut Universitaire de la Vigne et du Vin – Jules Guyot, F-21000 Dijon, 19 rue des Briquetiers, BP 59, 31 702 Blagnac, France

Contact the author

Keywords

Nucleophile, oxidation, wine, peptide, diversity

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Effect of polysaccharide extracts from grape pomace on the oxidative evolution of hydroxycinnamic acids

Phenolic acids are especially sensitive to oxidation, so they can greatly impact wine sensory characteristics and stability [1]. Furthermore, extracts derived from grape pomace have been previously postulated as possible oenological adjuvants for wine protection [2].

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.

Could intermittent shading, as produced in agrivoltaics, mitigate global warming effects on grapevine?

Global warning increases evaporative demand and accelerates grapevine phenology. As a consequence, the ripening phase shifts to warmer and drier periods. This results in lower acidity and higher sugar levels in berries, yielding too alcoholic wines with altered organoleptic properties. Agrivoltaics, which combines crop and renewable energy production on the same land using photovoltaic panels, emerged as a promising innovation to counteract these impacts by partially shading the plants.

Effect of oenological tannins on wine aroma before and after oxidation: a real-time study by coupling sensory (TDS) and chemical (PTR-ToF-MS) analyses

Polyphenols are important compounds involved in many chemical and sensory wine features. In winemaking, adding oenological tannins claims to have positive impacts on wine stability, protection from oxidation and aroma persistence. Polyphenols are antioxidant compounds by either scavenging reactive oxygen and nitrogen species or chelating Fe2+ ions (1). However, as tannins oxidation leads to the formation of highly reactive species (i.e. ortho-quinones), it is still unclear if they have an effective role toward oxidation of wine aromas (2). In this work, we aim at studying the effect of two commercial tannins (proanthocyanidins, ellagitannins) on red wine flavour (mainly aroma) before and after air exposition.

Correlations between N,S,O-heterocycle levels and age of Champagne base wines

Champagne regulation allows winegrowers to stock small amounts of still wines in order to compensate vintages’ quality shifts mainly due to climate variations. According to their technical requirements and house style some Champagne producers (commonly named “Champagne houses”) use these stored wines in the blend in order to introduce an element of complexity. These wines possess the particularity of being aged on fine lees in thermo-regulated stainless steel tanks. The Champagne house of Veuve Clicquot Ponsardin has several wines stored this way.