IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Peptides diversity and oxidative sensitivity: case of specific optimized inactivated yeasts

Peptides diversity and oxidative sensitivity: case of specific optimized inactivated yeasts

Abstract

Estimation of the resistance of a wine against oxidation is of great importance for the wine. To that purpose, most of the commonly used chemical assays that are dedicated to estimate the antioxidant (or antiradical) capacity of a wine consist in measuring the capacity of the wine to reduce an oxidative compound or a stable radical. In the must/wine matrix, polyphenols are major compounds likely to react with oxidant or radical, but such reaction generate quinones that then are involved in varietal aroma loss via nucleophilic addition reaction. It raises the paradox that a good antioxidant capacity does not imply a good protection of such sensitive compounds as aromatic compounds which are wine key quality markers.

The authors have developed a methodology focusing on the survival time of a sensitive compound to estimate the oxidative sensitivity of a solution. A labeled nucleophile is monitored by UHPLC-ESI-Q-ToF MS periodically for hours (from 0.5h to 72h) after a chemical initiation of oxidation in wine model solution containing 4-methylcatechol. 7 Cystein containing peptides (alone or in combination) are used to artificially increase the nucleophilic environment (and thus the competition for quinone nucleophilic addition) and estimate the half-life of the labeled nucleophile. In addition, soluble fraction released by different inactivated yeasts are also used as complex source of nucleophiles.

Half-life of the labeled compound is the final expression of several complex mechanisms such as nucleophilic competition, but also oxygen consumption, or catechol reduction.
Independently of the mechanisms, we can observe that increasing the nucleophilic environment improve the half life of the labeled nucleophile. It is notably visible for the specific optimized inactivated yeast which released many cysteine-containing peptides.
Finally, this method relies on the fate of one sensitive nucleophile to estimate the sensitiveness of the whole matrix to oxidation. It estimates the half-life of this compound which allows to compare oxidative sensitivity of different matrices under specific oxidation conditions.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Bahut Florian1, Sieczkowski Nathalie1, Nikolantonaki Maria1 and Gougeon Régis D.1

1Univ. Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, Lallemand SAS, Institut Universitaire de la Vigne et du Vin – Jules Guyot, F-21000 Dijon, 19 rue des Briquetiers, BP 59, 31 702 Blagnac, France

Contact the author

Keywords

Nucleophile, oxidation, wine, peptide, diversity

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Influence of grape withering on corvina and corvinone aroma composition

AIM:Valpolicella is a wine region located in Italy north-east, famous for the production of dry and sweet red wines from withered grapes, including Amarone and Recioto. The aim of this study is to understand the influence of the withering process on Corvina and Corvinone wines aroma profiles. METHODS:Wines were produced with a standard red wine winemaking protocol with Corvina and Corvinone grapes from different Valpolicella vineyards and vintages. In consideration of the local traditional practice of post-harvest withering of the grapes, wines from each vineyard were obtained from either fresh and withered grapes. Wines were analysed by Solid Phase Extraction and Solid Phase Micro Extraction gas chromatography coupled to mass spectrometry.

Investigating the carbon sequestration potential in vineyard soils–the SUSTAIN project

The SUSTAIN project aims at assessing the soil organic carbon (SOC) stock and vulnerability in vineyard in a climate change scenario.

Natural sparkling wine pétillant naturel: technological features and sensory profile

The article presents the results of a study on the technological features of producing sparkling wines of the Pétillant Naturel (Pet-Nat) type, made using the ancestral method from the Muscat Ottonel and Pinot Noir grape varieties.

Harnessing whole genome sequencing data to predict protein structure and function variation in grapevine

Grapevine (Vitis vinifera) is amongst the world’s most cultivated fruit crops, and of global and economic significance, producing a wide variety of grape-derived products, including wine, and table grapes. The genus Vitis, encompassing approximately 70 naturally occurring inter-fertile species, exhibits extensive genetic and phenotypic diversity, highlighted by the global cultivation of thousands of predominantly Vitis vinifera cultivars. Despite the importance of harnessing its naturally occurring genetic diversity to pursue traits of interest, especially considering the continued and growing demand for sustainable high-quality grape production, the systematic characterization of available functional genetic variants remains limited.

Impact of SO2 addition before alcoholic fermentation on the oxidative stability of Chardonnay white wines

Sulfites (SO2) addition during winemaking is a widespread practice worldwide. This addition is realized at different steps of the winemaking due to the antimicrobial and antioxidant capacity of SO2. In a context of understanding white wines oxidative stability, knowledge about the impact of SO2 on the wine molecular diversity, especially compounds involved in the antioxidant capacity of wine, appears to be very important. In recent years, some studies have shown that SO2 can react with a large number of wine compounds resulting in the formation of numerous adducts. The diversity of compounds involved is important including in particular pyruvic acid, 2-keto-glutaric acid, glyceraldehyde, sugar, phenolics compounds but also amino acids or peptides. Moreover Roullier-Gall et al. have shown using FT-ICR-MS analysis that the molecular composition of wines remains impacted by addition of SO2 to the must (0, 4 and 8 g/hL SO2), several years after winemaking. Indeed, wines made from protected must (8g/hL SO2) contain a larger diversity of CHOS and CHONS compounds than wines made from unprotected must (0 g/hL SO2). The study of the impact of glutathione addition on the sensory oxidative stability has further shown that CHOS and CHONS compounds (amino acids, aromatic compounds and peptides) are markers of the antioxidant metabolome of white wines. This suggests that CHOS and CHONS compounds arise from SO2 adducts formation but also from a protecting effect of SO2 on the antioxidant metabolome of white wines.