IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Abstract

Despite its relevance for wine quality and stability, red wine colloids have not still been sufficiently investigated, an occurrence due to the lack of suitable analytical techniques to study them as they are present in wine. Recently, asymmetrical Flow Field-flow Fractionation (AF4) with online multidetection has been tested as a new analytical tool to this aim, revealing its suitability for quantification, fractionation, and characterization of wine colloids in native state [1]. With the aim to characterize red wines in relation to their colloidal composition, AF4 technique was applied to 24 monovarietal Italian wines kept in bottles for 2 years and produced without any filtration, oak contact, fining treatments, malolactic fermentation or ageing on yeast lees. AF4 analysis allowed to quantify wine colloids, and to characterize them in terms of dimensions (by MALS) and absorbance (A280 & A520 nm). MALS revealed that each wine contained several colloids’ populations of different sizes (from 10 to 130 nm), but most of them showed sizes in the range 20 – 40 nm. The comparison by AF4 analysis of the A280-absorbing species present in whole wines with that of wines containing only species larger than 5 kDa (which were considered as colloids) allowed to calculate for each wine the percentage of molecules involved in the assembly of colloidal particles. This calculation showed that in the different samples the percentage of colloids varied from 1 to 44% of the total A280 absorbing compounds, indicating the diversity of the wines. Given that the A280 signal is mostly due to phenolics and proteins, these data indicate that very different percentages of these compounds participate in the formation of particles in the 20 – 40 nm size range. This means that phenolics necessarily need to be associated with other wine components to form particles of those dimensions. This association should involve proteins and polysaccharides [1]. The A520 data indicated the presence of pigments in the colloidal fraction. These pigments are likely to be constituted of tannin-anthocyanins complexes (polymeric pigments). Therefore, given the absence of species with sizes <20 nm, an association of these colored complexes with other colloidal-forming compounds seems necessary, the obvious candidate being proteins as they are known to strongly interact with tannins. Our results suggest that the color of red wines is due, in addition to free oligomeric pigments, also to colloidal particles formed by these latter bound to proteins, and that the quantity of these particles is highly variable in wines from different origin. How the presence of proteins affects the stability and evolution of red wines’ color remains to be investigated, keeping into consideration also the contribution of wine polysaccharides, which have been previously found to be part of the red wine colloidal particles [1].

References

[1] Marassi, et al. Food Hydrocoll 2021;110:106204.
Acknowledgments: MIUR project PRIN n.20157RN44Y

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Marangon Matteo1, Marassi Valentina2, Roda Barbara2, Zattoni Andrea2, Reschiglian Pierluigi2, Mattivi Fulvio3,4, Moio Luigi5, Parpinello Giuseppina Paola6, Piombino Paola5, Río Segade Susana7, Rolle Luca7, Slaghenaufi Davide8, Versari Andrea6, Vrhovsek Urska4, Ugliano Maurizio8 and Curioni Andrea1

1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
2Department of Chemistry “G. Ciamician”, University of Bologna, Italy
3Department of Cellular, Computational and Integrative Biology – CIBIO, University of Trento, Italy
4Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
5Department of Agricultural Sciences, Division of Vine and Wine Sciences, University of Napoli Federico II, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural, Forest and Food Sciences, University of Torino, Italy
8Department of Biotechnology, University of Verona, Italy

Contact the author

Keywords

red wine, colloids, proteins, polysaccharides, phenolics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Effects of organic mulches on the soil environment and yield of grapevine

Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Grape must quality and mesoclimatic variability in Fruška Gora wine-growing region, Serbia

The Fruška Gora mountain is a traditional wine-growing region in Serbia situated in the Pannonian Basin. Due to such a position, the vicinity of the Danube River and the presence of concave configuration, it is suitable for grape production. This paper provides analyses of spatial variations in meteorological parameters and grape juice quality within Fruška Gora wine region over three consecutive vintages (2018-2020). The examined period can be defined as warm with cool nights during September (AVG 18,9°C; GDD 1918°C; CI 12°CF) and with the presence of mesoclimatic variability. The East part of the study area was somewhat drier and hotter compared to other parts of the region. The analyses of grape must samples (190 in total) of five cultivars (Cabernet-Sauvignon, Merlot, Chardonnay, Sauvignon blanc and Grašac (Welschriesling)) commonly grown across the region (19 sites), were performed using Fourier Transform Infrared Technology (FTIR). Among all cultivars, Sauvignon blanc was harvested first in the East area (DOY=246±5, GDD at harvest=1552±74, 22.2±0.7 °Brix), while the latest harvest was recorded for Cabernet-Sauvignon in the West (DOY=283±5, GDD at harvest=1936±187, 23.4±1.0 °Brix ). Both the red and white cultivars had higher acidity and YAN in the grape must if the vines were grown in the North and East compared to South and West areas. According to PCA analysis, Grašac showed the lowest variation in grape must chemical composition. Thus, the results confirm that Grašac is the most stable cultivar in Fruška Gora. All monitored cultivars reached technological fruit ripeness by the end of the growing season. However, it was difficult to reach full ripeness of red cultivars, mostly beacuse of uncoupling of technolocical and phenolic ripeness. Thus, Cabernet-Sauvignon had higher variations in GDD sums at harvest compared to other cultivars, which probably increased variations in grape must quality.

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.