IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Abstract

Despite its relevance for wine quality and stability, red wine colloids have not still been sufficiently investigated, an occurrence due to the lack of suitable analytical techniques to study them as they are present in wine. Recently, asymmetrical Flow Field-flow Fractionation (AF4) with online multidetection has been tested as a new analytical tool to this aim, revealing its suitability for quantification, fractionation, and characterization of wine colloids in native state [1]. With the aim to characterize red wines in relation to their colloidal composition, AF4 technique was applied to 24 monovarietal Italian wines kept in bottles for 2 years and produced without any filtration, oak contact, fining treatments, malolactic fermentation or ageing on yeast lees. AF4 analysis allowed to quantify wine colloids, and to characterize them in terms of dimensions (by MALS) and absorbance (A280 & A520 nm). MALS revealed that each wine contained several colloids’ populations of different sizes (from 10 to 130 nm), but most of them showed sizes in the range 20 – 40 nm. The comparison by AF4 analysis of the A280-absorbing species present in whole wines with that of wines containing only species larger than 5 kDa (which were considered as colloids) allowed to calculate for each wine the percentage of molecules involved in the assembly of colloidal particles. This calculation showed that in the different samples the percentage of colloids varied from 1 to 44% of the total A280 absorbing compounds, indicating the diversity of the wines. Given that the A280 signal is mostly due to phenolics and proteins, these data indicate that very different percentages of these compounds participate in the formation of particles in the 20 – 40 nm size range. This means that phenolics necessarily need to be associated with other wine components to form particles of those dimensions. This association should involve proteins and polysaccharides [1]. The A520 data indicated the presence of pigments in the colloidal fraction. These pigments are likely to be constituted of tannin-anthocyanins complexes (polymeric pigments). Therefore, given the absence of species with sizes <20 nm, an association of these colored complexes with other colloidal-forming compounds seems necessary, the obvious candidate being proteins as they are known to strongly interact with tannins. Our results suggest that the color of red wines is due, in addition to free oligomeric pigments, also to colloidal particles formed by these latter bound to proteins, and that the quantity of these particles is highly variable in wines from different origin. How the presence of proteins affects the stability and evolution of red wines’ color remains to be investigated, keeping into consideration also the contribution of wine polysaccharides, which have been previously found to be part of the red wine colloidal particles [1].

References

[1] Marassi, et al. Food Hydrocoll 2021;110:106204.
Acknowledgments: MIUR project PRIN n.20157RN44Y

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Marangon Matteo1, Marassi Valentina2, Roda Barbara2, Zattoni Andrea2, Reschiglian Pierluigi2, Mattivi Fulvio3,4, Moio Luigi5, Parpinello Giuseppina Paola6, Piombino Paola5, Río Segade Susana7, Rolle Luca7, Slaghenaufi Davide8, Versari Andrea6, Vrhovsek Urska4, Ugliano Maurizio8 and Curioni Andrea1

1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
2Department of Chemistry “G. Ciamician”, University of Bologna, Italy
3Department of Cellular, Computational and Integrative Biology – CIBIO, University of Trento, Italy
4Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
5Department of Agricultural Sciences, Division of Vine and Wine Sciences, University of Napoli Federico II, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural, Forest and Food Sciences, University of Torino, Italy
8Department of Biotechnology, University of Verona, Italy

Contact the author

Keywords

red wine, colloids, proteins, polysaccharides, phenolics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Amino nitrogen content in grapes: the impact of crop limitation

As an essential element for grapevine development and yield, nitrogen is also involved in the winemaking process and largely affects wine composition. Grape must amino nitrogen deficiency affects the alcoholic fermentation kinetics and alters the development of wine aroma precursors. It is therefore essential to control and optimize nitrogen use efficiency by the plant to guarantee suitable grape nitrogen composition at harvest. Understanding the impact of environmental conditions and cultural practices on the plant nitrogen metabolism would allow us to better orientate our technical choices with the objective of quality and sustainability (less inputs, higher efficiency). This trial focuses on the impact of crop limitation – that is a common practice in European viticulture – on nitrogen distribution in the plant and particularly on grape nitrogen composition. A wide gradient of crop load was set up in a homogeneous plot of Chasselas (Vitis vinifera) in the experimental vineyard of Agroscope, Switzerland. Dry weight and nitrogen dynamics were monitored in the roots, trunk, canopy and grapes, during two consecutive years, using a 15N-labeling method. Grape amino nitrogen content was assessed in both years, at veraison and at harvest. The close relationship between fruits and roots in the maintenance of plant nitrogen balance was highlighted. Interestingly, grape nitrogen concentration remained unchanged regardless of crop load to the detriment of the growth and nitrogen content of the roots. Meanwhile, the size and the nitrogen concentration of the canopy were not affected. Leaf gas exchange rates were reduced in response to lower yield conditions, reducing carbon and nitrogen assimilation and increasing intrinsic water use efficiency. The must amino nitrogen profiles could be discriminated as a function of crop load. These findings demonstrate the impact of plant balance on grape nitrogen composition and contribute to the improvement of predictive models and sustainable cultural practices in perennial crops.

Towards adaptation to climate change in Rioja: Quality evaluation of wines obtained from Grenache x Tempranillo selections

The wine sector is of great relevance and tradition in Mediterranean countries, however, it may be most susceptible to climate change. In recent years, wine production is facing changes worldwide, both at environmental as well as commercial levels, due to global warming and the shift in consumers’ preferences. Wine growers and wine makers are in search of solutions that allow to face these new challenges. One of the most promising initiatives in the long term is the introduction of new plant materials, specifically intraspecific hybridizations between premium varieties that may improve traditional germplasm in its adaptation to climate change. These inter-varietal crosses have the potential to generate quality wines, whilst maintaining the regional typicity, and constitute an attractive alternative for the consumer due to their sensory attributes. In this study, we have evaluated wines from 29 intraspecific Garnacha x Tempranillo hybrids in two different locations, with the aim to assess their oenological potential and sensory attributes. Thirteen of the selections were white and 16 were red. Microvinifications were conducted with two or three replications depending on grape availability. Conventional oenological parameters were determined for all wines. The sensory evaluation and hedonic scores were given by five experts. Red selections obtained higher quality scores than white ones. Among the white selections with higher quality scores, GT-41 Varea and GT-159 Varea outstand, due to their high total acidity and high malic acid content. Regarding red selections, GT-57 Varea and GT-57 UR were perceived as higher in quality, highlighted for their moderate alcoholic and high anthocyanin content. Our results indicate that intraspecific hybridization may be a powerful tool for adapting traditional cultivars to climate change in Rioja.