IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Asymmetrical flow field-flow fractionation with online multidetection is a viable tool to investigate colored red wine colloids

Abstract

Despite its relevance for wine quality and stability, red wine colloids have not still been sufficiently investigated, an occurrence due to the lack of suitable analytical techniques to study them as they are present in wine. Recently, asymmetrical Flow Field-flow Fractionation (AF4) with online multidetection has been tested as a new analytical tool to this aim, revealing its suitability for quantification, fractionation, and characterization of wine colloids in native state [1]. With the aim to characterize red wines in relation to their colloidal composition, AF4 technique was applied to 24 monovarietal Italian wines kept in bottles for 2 years and produced without any filtration, oak contact, fining treatments, malolactic fermentation or ageing on yeast lees. AF4 analysis allowed to quantify wine colloids, and to characterize them in terms of dimensions (by MALS) and absorbance (A280 & A520 nm). MALS revealed that each wine contained several colloids’ populations of different sizes (from 10 to 130 nm), but most of them showed sizes in the range 20 – 40 nm. The comparison by AF4 analysis of the A280-absorbing species present in whole wines with that of wines containing only species larger than 5 kDa (which were considered as colloids) allowed to calculate for each wine the percentage of molecules involved in the assembly of colloidal particles. This calculation showed that in the different samples the percentage of colloids varied from 1 to 44% of the total A280 absorbing compounds, indicating the diversity of the wines. Given that the A280 signal is mostly due to phenolics and proteins, these data indicate that very different percentages of these compounds participate in the formation of particles in the 20 – 40 nm size range. This means that phenolics necessarily need to be associated with other wine components to form particles of those dimensions. This association should involve proteins and polysaccharides [1]. The A520 data indicated the presence of pigments in the colloidal fraction. These pigments are likely to be constituted of tannin-anthocyanins complexes (polymeric pigments). Therefore, given the absence of species with sizes <20 nm, an association of these colored complexes with other colloidal-forming compounds seems necessary, the obvious candidate being proteins as they are known to strongly interact with tannins. Our results suggest that the color of red wines is due, in addition to free oligomeric pigments, also to colloidal particles formed by these latter bound to proteins, and that the quantity of these particles is highly variable in wines from different origin. How the presence of proteins affects the stability and evolution of red wines’ color remains to be investigated, keeping into consideration also the contribution of wine polysaccharides, which have been previously found to be part of the red wine colloidal particles [1].

References

[1] Marassi, et al. Food Hydrocoll 2021;110:106204.
Acknowledgments: MIUR project PRIN n.20157RN44Y

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Marangon Matteo1, Marassi Valentina2, Roda Barbara2, Zattoni Andrea2, Reschiglian Pierluigi2, Mattivi Fulvio3,4, Moio Luigi5, Parpinello Giuseppina Paola6, Piombino Paola5, Río Segade Susana7, Rolle Luca7, Slaghenaufi Davide8, Versari Andrea6, Vrhovsek Urska4, Ugliano Maurizio8 and Curioni Andrea1

1Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
2Department of Chemistry “G. Ciamician”, University of Bologna, Italy
3Department of Cellular, Computational and Integrative Biology – CIBIO, University of Trento, Italy
4Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
5Department of Agricultural Sciences, Division of Vine and Wine Sciences, University of Napoli Federico II, Italy
6Department of Agricultural and Food Sciences, University of Bologna, Italy
7Department of Agricultural, Forest and Food Sciences, University of Torino, Italy
8Department of Biotechnology, University of Verona, Italy

Contact the author

Keywords

red wine, colloids, proteins, polysaccharides, phenolics

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Climate projections over France wine-growing region and its potential impact on phenology

Climate change represents a major challenge for the French wine industry. Climatic conditions in French vineyards have already changed and will continue to evolve. One of the notable effects on grapevine is the advancing growing season. The aim of this study is to characterise the evolution of agroclimatic indicators (Huglin index, number of hot days, mean temperature, cumulative rainfall and number of rainy days during the growing season) at French wine-growing regions scale between 1980 and 2019 using gridded data (8 km resolution, SAFRAN) and for the middle of the 21th century (2046-2065) with 21 GCMs statistically debiased and downscaled at 8 km. A set of three phenological models were used to simulate the budburst (BRIN, Smoothed-Utah), flowering, veraison and theoretical maturity (GFV and GSR) stages for two grape varieties (Chardonnay and Cabernet-Sauvignon) over the whole period studied. All the French wine-growing regions show an increase in both temperatures during the growing season and Huglin index. This increase is accompanied by an advance in the simulated flowering (+3 to +9 days), veraison (+6 to +13 days) and theoretical maturity (+6 to +16 days) stages, which are more noticeable in the north-eastern part of France. The climate projections unanimously show, for all the GCMs considered, a clear increase in the Huglin index (+662 to 771 °C.days compared to the 1980-1999 period) and in the number of hot days (+5.6 to 22.6 days) in all the wine regions studied. Regarding rainfall, the expected evolution remains very uncertain due to the heterogeneity of the climates simulated by the 21 models. Only 4 regions out of 21 have a significant decrease in the number of rainy days during the growing season. The two budburst models show a strong divergence in the evolution of this stage with an average difference of 18 days between the two models on all grapevine regions. The theoretical maturity is the most impacted stage with a potential advance between 40 and 23 days according to wine-growing regions.

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.