IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Predictive Breeding: Impact of véraison (onset of ripening) on wine quality

Predictive Breeding: Impact of véraison (onset of ripening) on wine quality

Abstract

Grapevine breeding focuses on high wine quality and climate-adapted grapevine varieties with fungal disease resistances to be cultivated in a pesticide-reduced and sustainable viticulture. While a number of resistance loci can be identified in marker-assisted selection (MAS), no adequate tools for an early detection of the highly important wine quality potential is available up to now. This is mainly due to the enhanced complexity of multifactorial traits and interrelated parameters. Implementation of quality traits to MAS has the potential to improve grapevine breeding efficiency considerably and is demanded by breeders. These traits bear the potential for an early negative selection of poor quality genotypes in recently germinated seedlings and could lead to an early identification of high quality genotypes in advanced breeding stages. In recent decades, the effects of global warming led to a well-documented earlier flowering and ripening in viticulture with strong impact on wine quality. A number of traditional grapevine cultivars show the tendency to ripen too early in most years in the wine growing regions of Germany. To deliver future climate adapted cultivars this has to be considered during selection.
The véraison called onset of ripening is characterized by berry softening, onset of sugar and aroma accumulation, switch from organic acid formation to degradation, and for red cultivars start of coloration. Thus, véraison marks the transition from berry growth to berry ripening.
Date of véraison was recorded for a ‘Calardis Musqué’ x ‘Villard Blanc’ white wine F1 population with 150 genotypes. Data of 17 individual datasets obtained over a period of 22 years and from three different field plots were included. Based on a genotyping-by-sequencing (GBS) approach and a novel bioinformatics pipeline to deliver highly informative haplotype-based markers (HBMs), a high density genetic map with 2,260 genome-wide distributed HBMs was used for quantitative trait loci (QTL) analysis.
The major QTL for véraison, Ver1, on chromosome 16, was validated. The improved data density and a locus-specific marker-densing (LSMD) approach narrowed down the postulated region from about 5 Mb with hundreds of genes to 174 kb encoding 13 genes including one strong candidate gene. Minor QTLs were observed on chromosomes 2, 7, 13, 17, and 18.
This knowledge is the starting point to develop suitable tools like MAS markers for grapevine breeding to select genotypes with the desired ripening time. In addition, unraveling the impact of véraison on quality determining constituents such as organic acids, sugars and aroma compounds will allow us to breed in a more targeted approach those new varieties, which are better adapted for future climatic conditions.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Schwander Florian1, Röckel Franco1, Frenzke Lena2, Wenke Torsten3, Siebert Annemarie4, Vestner Jochen4, Fischer Ulrich4, Wanke Stefan2 and Töpfer Reinhard1

1Julius Kühn-Institut (JKI), Institute for Grapevine Breeding Geilweilerhof
2Technische Universität Dresden, Institut für Botanik
3ASGEN GmbH & Co. KG
4DLR Rheinpfalz, Institute for Viticulture and Oenology

Contact the author

Keywords

Veraison, quantitative trait loci, haplotype-based markers, locus-specific marker-densing, marker-assisted selection

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Ecophysiological performance of Vitis rootstocks under water stress

The use of rootstocks tolerant to soil water deficit is an interesting strategy to cope with limited water availability. Currently, several nurseries are breeding new genotypes, but the physiological basis of its responses under water stress are largely unknown. To this end, an ecophysiological assessment of the conventional 110-Richter (110R) and SO4, and the new M1 and M4 rootstocks was carried out in potted ungrafted plants. During one season, these Vitis genotypes were grown under greenhouse conditions and subjected to two water regimes, well-watered and water deficit. Water potentials of plants under water deficit down to < -1.4 MPa, and net photosynthesis (AN) <5 μmol m-2 s-1 did not cause leaf oxidative stress damage compared to well-watered conditions in any of the genotypes. The antioxidant capacity was sufficient to neutralize the mild oxidative stress suffered. Under both treatments, gravimetric differences in daily water use were observed among genotypes, leading to differences in the biomass of root, shoot and leaf. Under well-watered conditions, SO4 and 110R were the most vigorous and M1 and M4 the least. However, under water stress, SO4 exhibited the greatest reduction in biomass while M4 showed the lowest. Remarkably, under these conditions, SO4 reached the least negative stem water potential (Ψstem), while M1 reduced stomatal conductance (gs) and AN the most. In addition, SO4 and M1 genotypes also showed the highest and lowest hydraulic conductance values, respectively. Our results suggest that there are differences in water use regulation among genotypes, not only attributed to differences in stomatal regulation or intrinsic water use efficiency at the leaf level. Therefore, because no differences in canopy-to-root ratio were achieved, it is hypothesized that xylem vessel anatomical differences may be driving the reported differences among rootstocks performance. Results demonstrate that each Vitis rootstock differs in its ecophysiological responses under water stress.

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.

A multidisciplinary approach to evaluate the effects of the training system on the performance of “Aglianico del Vulture” vineyards

Vineyards are complex agro-ecosystems with high spatial and temporal variability. An efficient training system may counteract the adverse effects of this variability. Moreover, considering the climate change issues, choosing an efficient training system that enhances water use and protects the vines from radiative thermal stress has become a priority for the farmers. A multidisciplinary approach that assesses the soil-crop-yield-wine relationships of vineyards in a distributed and holistic way could bring added knowledge on the behavior of the different training systems. This ongoing research aimed to implement a multidisciplinary approach to study the behavior of “Aglianico del Vulture” grapevines trained with two different systems: a spurred cordon (SC) and an “Alberello in parete” (AL), grown in a high-quality wine production area of Basilicata region (Italy). The approach merged several methods and scales of soil, ecophysiology, must/wine quality, and spectral data collection to assess the influence of the training system. Homogeneous zones (HZs) in both training systems were defined through a procedure based on geomorphological classification, unmanned aerial vehicles (UAV) images analysis, and a traditional soil survey supported by geophysical scanning. During the 2021 season, TDR probes monitored soil water content, while grapevine health status was assessed using eco-physiological measurements (LWP, chlorophyll content, PSII photosynthetic efficiency, LAI, and point-based field spectroscopy). These grapevine in-vivo measurements validated the spectral vegetation indexes (NDVI, RENDVI, CVI, and TVI) derived from the UAV multispectral imagery, which monitored the grapevine status in a distributed and non-invasive way. Grape yield, quality of berries, must and wine were measured to assess the effects of the training systems. The first experimental year results showed the variability of the vineyards and revealed relationships among soil parameters, crop characteristics, and vegetation indices of the SC and AL training systems. This multidisciplinary study could bring new insights into the vineyard training system’s effects on grape yield and wine quality.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.