IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 HPLC and SEC analysis on the flavonoids and the skin cell wall material of Merlot berries reveals new insights into the study of the phenolic maturity

HPLC and SEC analysis on the flavonoids and the skin cell wall material of Merlot berries reveals new insights into the study of the phenolic maturity

Abstract

Anthocyanins and tannins contribute to important sensorial traits of red wines, such as color and mouthfeel attributes. Despite the evolution of flavonoids during berry ripening has been extensively studied and the properties of skin and flesh cell wall material (CWM) to bind tannins were described, the mechanism determining the reduction of unpleasant astringency in the last phases of ripening remained uncertain. In this regard, the present research was aimed to better understand the factors involved in the phenolic maturity by a detailed evaluation of the flavonoid characteristics and the CWM properties, in the last weeks before harvest and at harvest. The study was conducted in 2014 and 2015 in a Merlot vineyard located in the hills near Bologna (north of Italy). The analysis of flavonoids and skin CWM was performed on berries sampled 20 and 10 days before harvest and at harvest. Exhaustive extractions were conducted to analyze total anthocyanins and tannins (skin and seed separately), while a model hydroalcoholic solution was used for their extractable portion. Moreover, binding reactions between the CWM and an enological seed tannin were performed to evaluate the magnitude of tannin precipitation and the composition of the  tannins remained in solution.HPLC analysis showed the increase of total and extractable anthocyanins during ripening, while no change was found in the concentration, composition and mean degree of polymerization (mDP) of skin and seed tannins. Also, the composition of CWM did not change significantly, but the tendency of proteins to increase until the harvest was noticed in both years. Moreover, CWM bound increasing quantity of the enological seed tannin during ripening, favoring, in particular, the precipitation of the tannins with higher mDP and of the galloilated forms, which are reported to be the compounds more involved in the perception of unpleasant astringency. The analysis performed by SEC confirmed that CWM bound preferentially the tannins of high molecular mass (MM), while lower amounts of medium MM tannins and negligible quantities of low MM tannins interacted with CWM. Our results confirmed that more ripen berries may release higher amounts of anthocyanins and allowed us to hypothesize that CWM may play a role in the decrease of astringency which is associated with the progression of ripening.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Allegro Gianluca1, Bautista-Ortín Ana-Belén2, Gómez-Plaza Encarna2, Pastore Chiara1,  Valentini Gabriele1, Mazzoleni Riccardo1 and Filippetti Ilaria1

1Department of Agricultural and Food Sciences – University of Bologna, Viale Fanin 46, 40127, Bologna, Italy
2Departamento de Tecnología de Alimentos, Nutrición y Bromatología – Universidad de Murcia (Spain)

Contact the author

Keywords

anthocyanins, astringency, ripening, tannins, Vitis vinifera

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...