IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Unravelling Saccharomyces cerevisiae biosynthethic pathways of melatonin, serotonin and hydroxytyrosol  by UPLC-HRMS Isotopic labelling analysis

Unravelling Saccharomyces cerevisiae biosynthethic pathways of melatonin, serotonin and hydroxytyrosol  by UPLC-HRMS Isotopic labelling analysis

Abstract

The main objective is to unravel the yeast biosynthetic pathways for MEL, SER and HT by using the respective labelled amino acids precursors: 15N2-L tryptophan and 13C-tyrosine.
The alcoholic fermentation experiments are performed with two different commercial S cereviseae yeasts using synthetic must with the addition of the labelled compounds and the bioactive compounds were followed during the fermentation process. Six biological replicates of the fermentations were considered. MEL, SER and HT were analysed by UHPLC coupled to High Resolution Mass Spectrometry (HRMS). Accurate mass determination allowed to unequivocally distinguishing labelled and unlabelled compounds. The analytical determination was performed using external calibration curves with 10 points, which were freshly prepared at every analytical session. Potential intermediates in the synthesis of MEL are detected as labelled metabolites following a time sequence that fits with the pathway described for the synthesis of MEL in mammals. At day 3 all the initial 15N2 L-TRP has been consumed. However, L-TRP as such was detected despite it was not added to the fermentation medium, thus demonstrating de novo tryptophan formation which can be used to synthetize MEL thereafter. Similarly, the results obtained in the fermentation carried out with 13-C TYR verify that HT is formed both from labelled tyrosine and from intermediates of the Erlich pathway.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Troncoso Ana M.1, Gallardo-Fernández Marta1, Valls-Fonayet Josep2, Valero Eva3, Hornedo-Ortega Ruth1, Richard Tristan2 and García-Parrilla M Carmen1 

1Universidad de Sevilla
2Université de Bordeaux
3Universidad Pablo de Olavide

Contact the author

Keywords

yeast, alcoholic fermentation, bioactive compounds, isotopically labelled compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Exploring magnesium defficiency in Welschriesling grapevines: A multi-omics approach to address viticultural challenges

Magnesium (Mg) deficiency poses a significant challenge to viticulture, particularly affecting Welschriesling (WR), a key grape variety in Austrian and Central European vineyards.

Developmental and genetic mechanisms underlying seedlessness in grapevine somatic variants

Seedless table grapes are greatly appreciated for fresh and dry consumption. There is also some interest in seedless winegrapes, because the combination of lower fruit set, smaller berries with higher skin/pulp ratio and looser bunches with the absence of seeds in crushed berries, a possible source of astringent tannins, might also have favorable effects on wine quality.
The gene VviAGL11 has been shown to play a central role in stenospermocarpy in Sultanina, but the molecular bases of other sources of stenospermocarpy as well as of parthenocarpy have not been clarified yet.

Interactions « Terroir x Vigne » : facteurs de maîtrise du micro-environnement et de la physiologie de la plante en rapport avec le niveau de maturité et les éléments de typicité

Le vigneron européen est de plus en plus à la recherche de la valorisation de son terroir par la personnalisation de la typicité de ses produits. Dans ce contexte, il est apparu depuis longtemps que la part des facteurs technologiques ou humains est d’une importance capitale face aux conditions de l’envirormement naturel. Le terroir se construit plus qu’il ne se subit.

PulvéLab: an experimental vineyard for innovation in precision spraying

One of the ways to reduce the use of pesticides is to adapt their dosage to the needs of the plant by using variable rate technology for managing field spatial variability. The recent evolution of technologies in the field of robotics, mechatronics and new information and communication technologies

Effect of the winemaking technology on the phenolic compounds, foam parameters in sparklig wines

Contribution Sparkling wines elaborated following the traditional method undergo a second fermentation in closed bottles of base wines, followed by aging of wines with lees for at least 9 months. Most of the sparkling wines elaborated are white and rosé ones, although the production of red ones is highly increasing. One of the initial problems in red sparkling wine processing is to obtain suitable base wines that should have moderate alcohol content and astringency and adequate color intensity; which is difficult to obtain when grapes must be harvested at low phenolic and industrial maturity stage. The low phenolic maturity degree in the red grapes makes essential to choose an adequate winemaking methodology to obtain the base wines because the extracted polyphenols will vary according the winemaking technique: carbonic maceration or destemmed-crushed grapes.