IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Unravelling Saccharomyces cerevisiae biosynthethic pathways of melatonin, serotonin and hydroxytyrosol  by UPLC-HRMS Isotopic labelling analysis

Unravelling Saccharomyces cerevisiae biosynthethic pathways of melatonin, serotonin and hydroxytyrosol  by UPLC-HRMS Isotopic labelling analysis

Abstract

The main objective is to unravel the yeast biosynthetic pathways for MEL, SER and HT by using the respective labelled amino acids precursors: 15N2-L tryptophan and 13C-tyrosine.
The alcoholic fermentation experiments are performed with two different commercial S cereviseae yeasts using synthetic must with the addition of the labelled compounds and the bioactive compounds were followed during the fermentation process. Six biological replicates of the fermentations were considered. MEL, SER and HT were analysed by UHPLC coupled to High Resolution Mass Spectrometry (HRMS). Accurate mass determination allowed to unequivocally distinguishing labelled and unlabelled compounds. The analytical determination was performed using external calibration curves with 10 points, which were freshly prepared at every analytical session. Potential intermediates in the synthesis of MEL are detected as labelled metabolites following a time sequence that fits with the pathway described for the synthesis of MEL in mammals. At day 3 all the initial 15N2 L-TRP has been consumed. However, L-TRP as such was detected despite it was not added to the fermentation medium, thus demonstrating de novo tryptophan formation which can be used to synthetize MEL thereafter. Similarly, the results obtained in the fermentation carried out with 13-C TYR verify that HT is formed both from labelled tyrosine and from intermediates of the Erlich pathway.

DOI:

Publication date: June 24, 2022

Issue: IVAS 2022

Type: Poster

Authors

Troncoso Ana M.1, Gallardo-Fernández Marta1, Valls-Fonayet Josep2, Valero Eva3, Hornedo-Ortega Ruth1, Richard Tristan2 and García-Parrilla M Carmen1 

1Universidad de Sevilla
2Université de Bordeaux
3Universidad Pablo de Olavide

Contact the author

Keywords

yeast, alcoholic fermentation, bioactive compounds, isotopically labelled compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Varietal differences between Shiraz and Cabernet sauvignon wines revealed by yeast metabolism

This study investigated if compositional differences between Shiraz and Cabernet Sauvignon grape varieties could influence the production of yeast-derived compounds. This work was based on the analysis of 40 experimental red wines made in triplicate fermentations from grapes harvested from two consecutive vintages in New South Wales (Australia). Grapes were picked at three maturity stages using berry sugar accumulation as physiological indicator, from nine commercial vineyards located in three different climatic regions (temperate, temperate-warm and warm-hot). A range of 30 yeast-derived wine volatiles including esters and alcohols were quantified by HS/SPME-GC/MS. Ammonia, amino-acids and lipids were analysed in the corresponding grapes. The juice total soluble solids (°Brix) in addition to the wine alcohol and residual sugar levels were also measured. The influence of grape maturity on wine ester composition was also variety dependent, particularly for higher alcohol acetate and ethyl ester of branched acids. This study highlights that varietal differences observed in Shiraz and Cabernet Sauvignon wines involve fermentation-derived compounds irrespective of the site (soil, climate, viticultural practices).

PERCEPTUAL INTERACTIONS PHENOMENA INVOLVING VARIOUS VOLATILE COMPOUND FAMILIES LINKED TO SOME FRUITY NOTES IN BORDEAUX RED WINES

Fruity notes play a key role in the consumer’s appreciation of Bordeaux red wines. If literature provides a lot of knowledge about the nature of volatile compounds involved in this fruity expression, the sensory phenomena involving these compounds in mixture still need to be explored. Considering previous sensory works about the impact of esters and some overripening compounds, the goal of this work was to study the implication of perceptual interactions involving red wine odorant compounds of diverse origins and described as potentially affecting fruity aromatic expression.

Coping with extreme climatic events: some lessons from recent work on grapevine under heat peak

Climate change critically challenges viticulture. Among other threats, extreme and increasingly frequent heatwaves cause irreversible burns on leaves and bunches. A series of observations and experiments was conducted to better understand how leaf burns originate and whether genetics or management practices can mitigate them. In 2019, a panel of 279 potted cultivars of Vitis vinifera L. grown outdoors suffered a heat peak and a genetic origin of leaf burn variability was demonstrated. To deeper explore this variability, fourteen cultivars were selected for their contrasting responses to high temperatures, and detached leaves were submitted to a controlled increase in temperature up to 50 °C in a growth chamber.

Untangling belowground response of grapevines to cover crop competition

Cover crops are planted in vineyards for multiple benefits including soil conservation, weed management, regulation of grapevine vegetative growth

Water availability at budbreak time in vineyards that are deficitary irrigated during the summer: Effect on must volatile composition


In recent years, Mediterranean regions are being affected by marked climate changes, primarily characterized by reduced precipitation, greater concurrence of temperature extremes and drought during the growing season, and increased inter-annual variability in temperatures and rainfall. Generally, high-quality red wines need moderate water deficit. Hence, irrigation may be needed to avoid severe vine water stress occurring in some vintages and soils with low holding capacity. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETO) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on must volatile composition at harvest.