Polyphenols in kombucha: impact of infusion time on extraction and investigation of their behavior during “fermentation”

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Phosphonic acid and especially potassium dihydrogen phosphonate are widely used to restrain the ubiquitous pressure of grapevine downy mildew in viticulture. Nevertheless, phosphonic acid and its derivatives have been banned in organic viticulture in October 2013, because they have been classified as plant protection products since then.

As the climate warms, the focus of concern in viticulture often turns to how higher temperatures may shift growing regions, change the character of AVAs, and alter fruit quality. However, climate warming is increasing most quickly during the winter dormancy cycle, a critical and often underappreciated portion of the grapevine life cycle. In response to decreasing temperatures and decreasing daylength, grapes initiate a series of physiological changes to enter dormancy, acquire freeze resistance, and time spring phenology such that the growing season begins after threat of frost.

The advent of precision viticulture (PV) has allowed to address problems related to spatial and temporal variability at the within-field scale. Nowadays, several remote and proximal sensing solutions allow description of the existing variability at different temporal and ground resolution through extremely robust soil, vigor, yield, and grape quality maps. In parallel, numerous studies have described grapevine performances within the homogeneous zones and identified soil as main driver of variability. There is a broad consensus that different vigor zones within the same plot may show differential canopy growth, yield and fruit composition, depicting diverse enological potentials and cultural needs.

Random sampling is often considered to be the best protocol for fruit sampling because it is assumed to produce a sample that best represents the vineyard population.