WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 3 - WAC - Posters 9 Preliminary characterisation of mannoproteins from different wine yeast strains and impact on wine properties

Preliminary characterisation of mannoproteins from different wine yeast strains and impact on wine properties

Abstract

Mannoproteins (MPs) are released from the yeast cell wall during alcoholic fermentation and aging on the lees, and influence aspects of wine quality such as haze formation and colour stability. Yet, as this is a slow process with microbiological and sensory risks, the exogenous addition of extracted MPs poses an efficient alternative. While Saccharomyces cerevisiae has long been studied as a prominent source for MPs extraction, their structure and composition greatly differ between yeast species. This may influence their behaviour in the wine matrix and subsequent impact on wine properties. However, although wine yeast species other than S. cerevisiae possibly present an untapped source of MPs, they are still ill-characterised in terms of chemical composition and influence on wine.

This study aimed to characterise the composition of MPs extracted from different wine yeast strains, and to investigate the impact of their addition on wine properties of organoleptic significance. MPs extracted and purified from four strains belonging to four different species (Saccharomyces cerevisiae, Saccharomyces boulardii, Metschnikowia fructicola and Torulaspora delbrueckii) were subjected to HR-SEC and GC-MS analyses to determine polysaccharide size distribution and monosaccharide composition, respectively. After addition of these MPs to Cabernet Sauvignon and Chardonnay wines, samples taken over the course of 6 months were analysed for protein stability, colour stability, browning potential and astringency.

HR-SEC analyses of the MPs revealed differences in size distribution patterns between species both in terms of the maximum and the medium molecular weight observed. MPs impact on the four different wine parameters tested were dependent on several factors, including time since MPs addition, and the yeast strain of origin. For example, whereas the addition of MPs from S. cerevisiae and S. boulardii showed increased white wine protein stability after 3 months, M. fructicola and T. delbrueckii did not.

The results obtained in this study form an important step towards further characterisation of mannoproteins derived from non-Saccharomyces yeast species. While the variable impact of MPs from different species on wine properties is an important aspect to consider in winemaking applications, further information regarding the influence of variations in MPs structure and composition could be used to better understand this effect.

DOI:

Publication date: June 27, 2022

Issue: WAC 2022

Type: Article

Authors

Carla Snyman, Nathalie Sieczkowski, Matteo Marangon, Benoit Divol

Presenting author

Carla Snyman – South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa

Lallemand SAS, 19 rue des briquetiers, BP 59, 31702 Blagnac, France | Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale Dell’Università, 16, 35020, Legnaro, Padova, Italy | South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa

Contact the author

Keywords

Non-Saccharomyces – mannoprotein – characterisation – HR-SEC – wine properties

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Le aree viticole storiche nel mondo: i loro vitigni, la loro protezione e la tipicità dei vini in esse ottenuti

Il tema da trattare si riferisce ai vari ecosistemi viticoli mondiali, ovviamente non facilmente sintetizzabili in una relazione. Sostanzialmente si richiama

The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci. 22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity.

WINE CONSUMER TRADE-OFF BETWEEN ORGANOLEPTIC CHARACTERISTICS AND SUSTAINABLE CLAIMS. AN EXPERIMENT ON RED WINES FROM BORDEAUX REGION

In economics, the perception of wine quality is not limited to sensorial characteristics: an indication of the region of production significantly affects the perception of quality and consumers’ WTP ([1]; [2]). However, [3] or more recently [4] show that even if a wine has an organic label, the taste of wine remains the predominant criterion in consumer preferences. The contribution of our experiment is to evaluate the impact of responsible attributes (organic label, Non Added Sulfites, HVE certification) on the appreciation of several red wines on the market. More than 280 consumers participated to the present study and they perform 25 tastings divided into 5 different sessions. 20 different red wines from Bordeaux Area are tasted.

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane.

The exploitation of Croatian grapevine genetic resources for the breeding of new resistant cultivars 

Croatian viticulture is mainly based on native grapevine varieties susceptible to various diseases and pests, which leads to unsustainable use of large amounts of pesticides. The sustainable development of viticulture in the future will only be possible by increasing the resistance of the grapevine through the development of new resistant varieties. Breeding programs have been launched in the leading wine-growing countries to develop resistant varieties possessing high-quality levels. Native cultivars from Croatia are not included in the breeding programs of other countries.