GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Mapping plant water status to indirectly assess variability in grape flavonoids and inform selective harvest decisions

Mapping plant water status to indirectly assess variability in grape flavonoids and inform selective harvest decisions

Abstract

Context and purpose of the study – Plant water stress affects grape (Vitis vinifera L.) berry composition and is variable in space due to variations in the physical environment at the growing site. Could we use water status maps as a sensitive tool to discriminate between harvest zones?

Material and methods – The study was carried out on 35experimental units placed on an equidistant grid within a 3.5 ha vineyard located in Sonoma County, Northern California. This drip irrigated vineyard was planted with Cabernet-Sauvignon on 110R, spur pruned and trained in two single high wires in a horizontally split canopy. The site was described through a digital elevation model, terrain analysis, NDVI and electrical resistivity maps. The natural variability of grapevine water stress was monitored by stem water potential (Ψstem), leaf gas exchange and δ13C of grape must at harvest. Geospatial analysis and clustering were used to differentiate the vineyard block into two management zones according to variability in water status.

Results – The two management zones were very distinct in water status and presented severe and moderate water stress. The average difference in Ψstem between the zones was of 0.2 MPa. Differences in stem water potential affected stomatal conductance, net carbon assimilation, and intrinsic water use efficiency that were different in all measurement dates. The two zones were selectively sampled at harvest for measurements of berry chemistry. Berry mass and yield per vine in the two water status zones were not different. A significant difference in total soluble solids (3.56 Brix) and in titratable acidity indicated a direct effect of water stress on ripening acceleration. Berry skin flavonol and anthocyanin composition and concentration were measured by C18 reversed-phased high-performance liquid chromatography (HPLC). Berry anthocyanins showed the highest differences between the two water stress zones. Dihydroxylated anthocyanins were more affected than the trihydroxylated ones, therefore, the ratio of the two forms increased. Flavonols were different in total amounts, but hydroxylation patterns were not affected. Proanthocyanidin isolates were characterized by acid catalysis in the presence of excess phloroglucinol followed by reversed-phase HPLC. Proanthocyanidins showed the least significant difference, although (+)-catechin terminal subunits were important predictors in a partial least square model used to summarize the multivariate relationships, predicting Ψstem or the management zone. The results highlight the importance of vineyard water status information for differential harvesting or direction to vineyard operators to modify irrigation management to equilibrate berry composition at harvest.

DOI:

Publication date: September 27, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Luca BRILLANTE1*, Luis SANCHEZ2, Johann MARTINEZ-LUSHER3, Runze YU3, S. Kaan KURTURAL3

1 Dep. of Viticulture and Enology, California State University, Fresno, CA 93740. USA
2 E. J. Gallo Winery, 1541 Cummins Drive, Modesto, California 95358, United States
3 Department of Viticulture and Enology, University of California, Davis, CA 95616, USA

Contact the author

Keywords

selective harvest, spatial variability, management zones, water stress, anthocyanins, flavonols, proanthocyanidins

Tags

Citation

Related articles…

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

An analytical framework to site-specifically study climate influence on grapevine involving the functional and Bayesian exploration of farm data time series synchronized using an eGDD thermal index

Climate influence on grapevine physiology is prevalent and this influence is only expected to increase with climate change. Although governed by a general determinism, climate influence on grapevine physiology may present variations according to the terroir. In addition, these site-specific differences are likely to be enhanced when climate influence is studied using farm data. Indeed, farm data integrate additional sources of variation such as a varying representativity of the conditions actually experienced in the field. Nevertheless, there is a real challenge in valuing farm data to enable grape growers to understand their own terroir and consequently adapt their practices to the local conditions. In such a context, this article proposes a framework to site-specifically study climate influence on grapevine physiology using farm data. It focuses on improving the analysis of time series of weather data. The analytical framework includes the synchronization of time series using site-specific thermal indices computed with an original method called Extended Growing Degree Days (eGDD). Synchronized time series are then analyzed using a Bayesian functional Linear regression with Sparse Steps functions (BLiSS) in order to detect site-specific periods of strong climate influence on yield development. The article focuses on temperature and rain influence on grape yield development as a case study. It uses data from three commercial vineyards respectively situated in the Bordeaux region (France), California (USA) and Israel. For all vineyards, common periods of climate influence on yield development were found. They corresponded to already known periods, for example around veraison of the year before harvest. However, the periods differed in their precise timing (e.g. before, around or after veraison), duration and correlation direction with yield. Other periods were found for only one or two vineyards and/or were not referred to in literature, for example during the winter before harvest.

Ripening characterization and modelling of Listan negro grape in Spain using a regression analysis

The professional winegrower usually selects the harvest date considering several elements, such as the vine stem and berry colour, the flavour, appearance and grain elasticity. Nowadays these elements have turned old fashioned.

Influence of grapes origin and yeast strain on aroma profile of corvina and corvinone dry passito wines

Valpolicella is a wine region characterized by a wide use of the technology of grape drying for the production of two red passito wines, recognized as PDOs, “Recioto della Valpolicella” and the most famous “Amarone della Valpolicella”. Geographical origin of the grapes can influence wine composition by grape chemical composition yeast behaviour during fermentation. This study investigates the impact of different commercial yeast strains on aroma profiles of wines produced with withered grapes of different origins. In addition, the influence of spontaneous fermentation is also considered. METHODS: Experimental red wines were produced with a standard winemaking protocol with withered Corvina and Corvinone grapes obtained from two different geographical areas within the Valpolicella region. Fermentations were carried out with four different commercial yeasts plus a spontaneous fermentation. Wines were analysed by means of SPE- and SPME-GC-MS techniques and sensory analysis (sorting task).

Effect of oenological tannins on wine aroma before and after oxidation: a real-time study by coupling sensory (TDS) and chemical (PTR-ToF-MS) analyses

Polyphenols are important compounds involved in many chemical and sensory wine features. In winemaking, adding oenological tannins claims to have positive impacts on wine stability, protection from oxidation and aroma persistence. Polyphenols are antioxidant compounds by either scavenging reactive oxygen and nitrogen species or chelating Fe2+ ions (1). However, as tannins oxidation leads to the formation of highly reactive species (i.e. ortho-quinones), it is still unclear if they have an effective role toward oxidation of wine aromas (2). In this work, we aim at studying the effect of two commercial tannins (proanthocyanidins, ellagitannins) on red wine flavour (mainly aroma) before and after air exposition.