OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Fungal resident flora of a new winery: colonization, dynamics and potential persistence capacities

Fungal resident flora of a new winery: colonization, dynamics and potential persistence capacities

Abstract

Through the years, extensive studies have been conducted on fungal biodiversity during the winemaking process: from the vineyard until aging. More recently, and as previously described for Saccharomyces cerevisiae, the persistence of winery resident flora (non-Saccharomyces yeasts) over time and its contribution to the alcoholic fermentation have been demonstrated. Also, winery surfaces were described as a true ecological niche and a transitional habitat for this resident flora. 

To our knowledge, no study has been done on the evolution and persistence of indigenous yeast flora in a new winery nor on the capacities of this indigenous flora to persist in the winery environment. Thus, the first objective of this work is to study the diversity and to follow the evolution of fungal communities in a new established winery operating only with indigenous yeast flora and for a period of 3 vintages (2016, 2017 and 2018). For this purpose, samples were collected from three different winery surfaces (soil, walls and equipment), at separate time points (before grape harvest, during the fermenting phase and 3 months after the fermentations) and were analyzed using the Mi-Seq sequencing. In a second objective, genetic diversity, persistence in winery environments and the implantation in must /or wine of indigenous S. cerevisiae isolates were monitored for all the collected samples using microsatellites PCRs. In addition, the killer character and biofilm formation of different isolated strains were investigated to determine potential capacities to persist in winery environments. 

The results obtained showed a high fungal diversity (yeasts, fungi and mold) on the 3 winery environments even before the first grape harvest (2016). As for yeasts, previously described genera (Candida, Metschinikowia, Rhodotorula, Saccharomyces, Wickerhamomyces, …) have been identified on winery surfaces but also yeast genera (Buckleyzyma, Curvibasidium, Leucosporidium, …) that have not been before described in the winemaking process. Then, the observed fungal diversity showed evolution over time and dependently according to each of the studied environment. Additionally, some fungal equilibria appears to take place between genera such as Aureobasidium, Candida and Wickerhamomyces. 

Concerning indigenous S. cerevisiae strains, our results demonstrated the potential implantation and persistence of some strains present in the winery environment during 2017 and 2018 vintages and during the alcoholic fermentations. Thus, selected strains of indigenous S. cerevisiae seem to have different physiological characteristics that could explain their potential persistence in winery environments.

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Hany Abdo 1, Claudia Rita Catacchio 2, Mario Ventura 2, Julie Laurent 1, Hervé Alexandre 1, Michele Guilloux-Benatier 1, Sandrine Rousseaux 1

1. Univ. Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon, France
2. Department of Biology, University of Bari, Bari 70125, Italy

Contact the author

Keywords

Fungal diversity and dynamics, New winery, Fungal resident flora, Saccharomyces cerevisiae

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.

Optimizing the use of bentonite for better control of haze formation In white and rosé wines

In winemaking, the appearance of turbidity in white and wine is a serious visual defect, which lowers significantly its commercial value. A major cause of the formation of turbidity in wine is attributed to the presence of temperature-sensitive proteins.

Disease‐induced alterations in the reflectance spectrum of grape leaves

Context and purpose of the study ‐ Phytopathogenic diseases impact the development and yield of grapevines, resulting in economical, social and environmental losses.

BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product.

Caractérisation des relations hydriques sol/vigne dans un terroir languedocien

Par le fait d’une politique agricole communautaire axée sur des objectifs de qualité des produits, la recherche et l’identification des critères de cette qualité deviennent impératives. En viticulture, la notion de qualité du produit est rattachée au concept théorique de «terroir». Ce terme englobe un ensemble de paramètres du milieu (géologie, sol, climat) influant sur la récolte.