GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Isohydric and anisohydric behavior of 18 wine grape varieties grown in an arid climate

Isohydric and anisohydric behavior of 18 wine grape varieties grown in an arid climate

Abstract

Context and purpose of the study – The interest in understanding the water balance of terrestrial plants under drought has led to the creation of the isohydric/anisohydric terminology. The classification was related to an implication-driven framework, where isohydric plants maintain a constant and high leaf water potential through an early and intense closure of their stomata, hence risking carbon starvation. In contrast, anisohydric plants drop their leaf water potential to low values as soil drought is establishing due to insensitive stomata and thus risk mortality through hydraulic failure, albeit maximizing carbon intake. When applied to grapevines, this framework has been elusive, yielding discrepancies in the classification of different wine grape varieties around the world. There is a need to assess different wine grape varieties under the same growing conditions to enable conclusions on the differences in their response to drought and facilitate variety-specific irrigation management.

Material and methods – The vineyard was located in the ROZA irrigation district in the Yakima valley, Washington. Varieties were grown side by side and replicated 8 times. Spacing was 1.8 m x 2.7 m in a North-South orientation. The vines were on their own-roots, double-trunked, trained to a bi-lateral cordon. 12-18 varieties of wine grape grown were studied for this experiment. Access tubes were installed for soil moisture measurements using a neutron probe, and irrigation was independently controlled for each row. Dry-down cycles were applied pre- and post-veraison from 2016 to 2018. On the same day, predawn (Ψpd) and midday leaf water potential (Ψmd) were measured with a pressure chamber, stomatal conductance (gs) was measured with a porometer at midday and on the same leaf in 2016 and 2017 and with an infrared gas analyzer in 2018. Soil moisture measurements were taken on the same day for each vine.

Results – The results show that there may be three distinctive major patterns of midday leaf water potential response to soil water availability: Linear drop across the entire soil moisture range such as for Cabernet franc and Semillon, linear drop below a threshold of soil moisture such as for Gewurztraminer and Grenache, and an insensitive to soil moisture such as for Lemberger and Riesling. Meanwhile, the stomatal sensitivity did not always mirror the Ψmd behavior; for example some varieties like Cabernet franc show a linear drop of Ψmid while having a tight stomatal control during soil drought (r=0.76) while other varieties like Riesling have an insensitive response of Ψmid (r=0.33) without necessarily having sensitive stomata (r=0.56). Finally, the slope of the linear Ψmd:Ψpd, studied as an the indicator of the internal regulation of water status, varied between 0.4 for Grenache and 1 for Semillon. This shows that for our vineyard, transpiration sensitivity was always higher than hydraulic sensitivity. Since intense yellowing of leaves has been recorded in varieties like Cabernet franc, Muscat blanc and Malbec, these results direct us to inspect if the sensitivity of gs in those varieties is leading to carbon starvation during drought. These results may eventually be used by growers to devise variety-specific irrigation management strategies.

DOI:

Publication date: September 28, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Joelle MARTINEZ*, Markus KELLER

Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA, USA

Contact the author

Keywords

wine grape, Isohydric, Anisohydric, stomatal regulation, water potential, hydraulic regulation

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Metabolomic discrimination of grapevine water status for Chardonnay and Pinot noir

Water status impact in viticulture has been widely explored, as it strongly affects grapevine physiology and grape chemical composition. It is considered as a key component of vitivinicultural terroir. Most of the studies concerning grapevine water status have focused on either physiological traits, or berry compounds, or traits involved in wine quality. Here, the response of grapevine to water availability during the ripening period is assessed through non-targeted metabolomics analysis of grape berries by ultra-high resolution mass spectrometry. The grapevine water status has been assessed during 2 consecutive years (2019 & 2020), through carbon isotope discrimination on juices from berries collected at maturity (21.5 brix approx.) for 2 Vitis vinifera cv. Pinot noir (PN) and Chardonnay (CH). A total of 220 grape juices were collected from 5 countries worldwide (Italy; Argentina; France; Germany; Portugal). Measured δ13C (‰) varied from -28.73 to -22.6 for PN, and from -28.79 to -21.67 for CH. These results also clearly revealed higher water stress for the 2020 vintage. The same grape juices have been analysed by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Liquid Chromatography coupled to Mass Spectrometry (LC-qTOF-MS), leading to the detection of up to 4500 CHONS containing elemental compositions, and thus likely tens of thousands of individual compounds, which include fatty acids, organic acids, peptides, phenolics, also with high levels of glycosylation. Multivariate statistical analysis revealed that up to 160 elemental compositions, covering the whole range of detected masses (100 –1000 m/z), were significantly correlated to the observed gradients of water status. Examples of chemical markers, which are representative of these complex fingerprints, include various derivatives of the known abscisic acid (ABA), such as phaesic acid or abscisic acid glucose ester, which are significantly correlated with higher water stress, regardless of the variety. Cultivar-specific behaviours could also be identified from these fingerprints. Our results provide an unprecedented representation of the metabolic diversity, which is involved in the water status regulation at the grape level, and which could contribute to a better knowledge of the grapevine mitigation strategy in a climate change context.