OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Mathematical modeling of fermentation kinetics: a tool to better understand interactions between Torulaspora delbrueckii and Saccharomyces cerevisiae in mixed cultures

Mathematical modeling of fermentation kinetics: a tool to better understand interactions between Torulaspora delbrueckii and Saccharomyces cerevisiae in mixed cultures

Abstract

Nowadays the use of Torulaspora delbrueckii is more and more common in winemaking. However, its behavior in presence of Saccharomyces cerevisiae is not always predictable. Indeed, the interactions existing between the two yeasts are still not well characterized and can lead to a bad control during their implementation in mixed cultures. The objective of the work presented here was to use the mathematical modeling as a tool to better understand microbial interactions in this context. 

Mixed cultures of a couple of oenological yeasts composed of T. delbrueckii and S. cerevisiae were carried out on a synthetic grape must in anaerobiosis. The impact of various parameters was evaluated: assimilable nitrogen concentration, direct and indirect contact (thanks to a membrane bioreactor), increase of lipids concentration (Tween 80 and ergosterol). 

The analysis of experimental data acquired during the pure cultures of each yeast enable to establish a mathematical model to describe the fermentation kinetics for pure cultures. Then this model was used to predict the kinetics of mixed cultures without any interaction except competition for substrates (sugar and nitrogen). The comparison between predicted and experimental kinetics showed that in mixed culture several kind of interactions must be taken into account: competition for space, cell to cell contact, reciprocal stimulation. Moreover, at low lipids initial concentration, S. cerevisiae dominated T. delbrueckii by producing a toxic metabolite. An increase in the initial lipids concentration completely reversed this domination.

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Patricia Taillandier, Cedric Brandam, Sandra Beaufort, Paul Brou

LGC université de Toulouse – 4 alle Emile Monso CS 84234 – 31432 Toulouse Cedex4

Contact the author

Keywords

modeling, interaction, Saccharomyces, Torulaspora 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Copper, iron and zinc in surface layer of Primošten vineyard soils

Long-term use of copper fungicides causes increased accumulation of total copper in the surface layer of vineyard soils. Many of authors has researched the anthropogenic influx of copper in such soils, which can result in environmental risks.

Trends and challenges in International Wine Trade. The need for new strategies for companies and regions.

Trends already extended for more than 12 years show a decline in both consumption and international trade, particularly in volume. However, there are also positive signs in several categories of wine, segments and markets, as well as a better trend in terms of value. How are these trends affecting wine producers and distributors? Are they short or long term? do they mean radical and permanent changes to which a way of adaptation has to be found or are they just temporary changes that may only require some calm? How are companies adapting to these new trends? Which are their effects on wine regions?

Exploring multisensory interactions through the study of astringency diversity of mono-varietal Italian red wines

According to the OIV Focus 2017 estimating the vine varieties distribution in the world, Italy is the richest grape producing country in terms of varieties.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

The influence of pre-heatwave leaf removal on leaf physiology and berry development

Due to climate change, the occurrence of heatwaves and drought events is increasing, with significant impact on viticulture. Common ways to adapt viticulture to a changing climate include site selection, genotype selection, irrigation management and canopy management. The latter mentioned being for instance source-sink manipulations, such as leaf removal, with the aim to delay ripening.