GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Switch genes as a key to understand the grapevine ripening disorder berry Shrivel?

Switch genes as a key to understand the grapevine ripening disorder berry Shrivel?

Abstract

Context and purpose of the study – The ripening of grapevine berries encompasses complex morphological and physiological processes, especially at veraison. Berry shrivel (BS) is a ripening physiological disorder affecting grape berries with visible symptoms appearing short after veraison. The main symptoms of BS are a strong reduction in sugar accumulation, inhibited anthocyanin biosynthesis and high pH values. The most popular red grape cultivar in Austria “Blauer Zweigelt” (Vitis vinifera L.) is specifically prone to develop the BS ripening disorder and up to date a no specific cause or causes could be identified. Recently omics approaches have identified and characterized key processes during grapevine ripening. Among them a small subset of genes, called SWITCH, have been described as markers for the onset of the ripening process in fruits. The peculiarity of a switch gene is to be expressed in a low level during the immature/green phase of development, to switch on at the onset of ripening and being significantly induced and highly expressed during the mature/ripening phase.

Material and methods – In this study, for the first time a transcriptomic analysis was undertaken to understand the metabolic modifications induced by the disorder. Different stages of berry development were considered including pre- and symptomatic berries.Samples selected for analyses were collected at 30, 44, 51, 58, 65, and 72 DAA. 50% veraison occurred approximately at 55 DAA.

Results – Different stages of berry development were considered including pre- and symptomatic berries. The total number of differentially expressed genes (DEG) between the two conditions was 3122. The number of DEG in the three pre-veraison samples was 0, 1, and 0; while the number of DEG modulated by BS in post-veraison was 297 (75 up-regulated, 222 down-regulated) at 58 DAA, 1489 (955 up-regulated, 534 down-regulated) at 65 DAA and 2452 (1415 up-regulated, 1036 down-regulated) at 72 DAA. During this key transition (58 DAA at veraison) we could identify 297 DEG. Most of them (75%) were down-regulated in BS. Among the 190 grapevine switch genes, at 58 DAA we identified 67 switch genes differentially expressed; all of them were down-regulated in BS samples. Our results showed no metabolic alterations in pre-symptomatic and pre-veraison samples. Interestingly, at veraison, with still not visible symptoms appearing on the berry, a subset of switch genes previously suggested as master regulators of the ripening onset in grape berries, were strongly lower expressed in BS. Later during the ripening phase and with visible symptoms of the disorder, more than 3000 genes were differentially expressed. Most of them with significant lower expression during ripening belonged to the flavonoid pathway, and others were involved in the sugar metabolism. In conclusion, these results highlight a pivotal role of the switch genes in grapevine ripening, as well as their possible contribution to induce the ripening disorder berry shrivel, although it remains unclear whether this is part of the cause or consequences of the BS disorder.

DOI:

Publication date: September 20, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Michaela GRIESSER1*, Stefania SAVOI1,2, Jose Carlos HERRERA1, Astrid FORNECK1

1 Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Straße 24, 3430 Tulln, Austria

2 Current address: Montpellier SupAgro, 2 Place Pierre Viala, 34060 Montpellier, France

Contact the author

Keywords

grapevine ripening, Berry Shrivel, SWITCH genes, physiological disorders

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Valorization of wine lees for oenological interest by eco-responsible processes

Wine lees are the second most important wine by-product in terms of quantity after grape stalk and marc. During aging on lees, it is well known that wine lees yield compounds that act as antioxydant. However the chemical nature of the compounds involved in this behavior (except polyphenols and glutathione) has not yet been totally elucidated. The scarce knowledge of wine lees composition and their potential exploitation make them a promising candidate to obtain new antioxidant products to be used in winemaking. In this study, an eco-sustainable approach to obtain lees extracts exhibiting antioxidant capacity is proposed. Such extracts could be used in a global enological strategy of sulfites level reduction.

Nitrogen uptake, translocation and YAN in berries upon water deficit in grapevines with contrasting stomatal sensitivity

Nitrogen (N2) is critical in grape berries, especially in organic wine making. After intake, N2 follows various metabolic and allocation routes and, from veraison, partly reallocates into berries. Water deficit affects the N2 nutrition due to a poor diffusion in soil solution and vascular mobilisation. Also, affects photosynthesis and the energy needed for metabolism, whose extent would depend on the stomatal sensitivity of the plant. We have assessed the effect of a moderate water deficit from pea size, in 3 years old field grown potted plants of Chardonnay (CH) and Cabernet Sauvignon (CS), differing in stomatal sensitivity, on the N2 status of plant parts. Water deficit reduced photosynthesis, leaf area and fresh and dry plant mass along the season, but up to a higher extent in CS.

Local ancient grapevine cultivars to face future viticulture

Among the different strategies to cope with the negative impacts of climate change on viticulture, the exploitation of genetic diversity is one of the most promising to adapt to new conditions and maintain wine production and quality. One of the biggest concerns in the context of climate change is to improve water use efficiency (WUE). In this way, the use of genotypes that present a better response to drought and high WUE is a key issue. In this work, physiological performance analysis was conducted to compare the water deficit stress (WDS) responses of local and widespread grapevines cultivars. Leaf gas exchange, water use efficiency (WUE) at different levels (leaf and long-term WUE (∆13C)), leaf osmotic adjustment and other water relations parameters were determined in plants under well-watered and WDS conditions alongside assessment of the levels of foliar hormones concentrations. Results denote that local cultivars displayed better physiological performance under WDS as compared to the widely-distributed ones. he results corroborate the hypothesis that better stomatal control allows increasing leaf WUE under drought as occurred in the local Callet cv.; but the minority local cultivar Escursac cv. showed high WUE under both treatments. In this case, high WUE can be related to maintaining higher photosynthetic activity under drought. The different mechanisms underlying the better performance under WDS and high WUE of minority local cultivars are discussed.

La zonazione della D.O.C. Bolgheri (Castagneto C.): aspetti metodologici ed applicativi

The results of the first step of the zoning study carried out in Bolghery appellation area (Castagneto Carducci, Tuscany) in the 1993-1995 period have been recently published. Quality factors of Bolgheri appellation and different “terroirs ” were identified.