OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Abstract

Foam is a key aspect of quality of sparkling wines. Bentonite is usually added to the wine to prevent protein haze, but reducing its foamability [1]. New skills are searching to avoid this undesirable event [2]. Acacia senegal gum (Asen) is an exudate from Acacia trees, which can be used to stabilize red wine color. Asen can be fractionated, and the most widely used method is Hydrophobic Interaction Chromatography (HIC) to obtain low (HIC-F1), medium (HIC-F2) and high (HIC-F3) molar mass fractions. The effect of these fractions on the foamability of bentonite-treated wines was studied, showing positive or negative effects depending on the fraction and the wine [3].

Asen can also be fractionated by Ion Exchange Chromatography (IEC) giving a high (IEC-F1) and low (IEC-F2) molar mass fractions [4]. A synthetic wine (SYWI) was prepared (12 % v/v ethanol, 3 g·L-1 of tartaric acid). 8 base wines from Spain (3) and France (5) were made by the traditional white winemaking method. They were treated with bentonite (20 g·hL-1), stirred gently for a few hours, kept in cold storage (10 days, 4 °C), racked and filtered (1 μm). IEC-fractions were added to SYWI (60 g·hL-1) and to wines (30 and 10 g·hL-1). The foaming parameters were compared by shake test and by a classical gas-sparging method (Mosalux), being the qualitative aspect of foam also observed.

In SYWI, IEC-F1 improves the foamability during the total shake test. Both fractions enhance its Maximum Foam Height (HM) and the Foam Stability Height at 5 minutes (HS) measured by Mosalux. IEC-F1 provides less compact foam with larger bubble. In Spanish wines, IEC-F1 increases the foamability during the total shake test. IEC-F1 also improves it in French wines, but weaker and differently depending on the wine. The foamability is punctually enhanced by IEC-F2 in some wines, but it is greatly decreased in 1 French wine. The dose reduction decreases the improving impact of IEC-F1 on the foamability of the French selected wine but not in the Spanish selected wine. IEC-F1 increases HM and HS in both selected wines, whereas IEC-F2 improves HS only in the Spanish selected wine.

Concluding, the addition of IEC-F1 increases foamability for all the studied wines, but very differently depending on the wine. IEC-F2 addition shows positive, neutral or even negative effects depending on the wine. Dose of IEC-F1 may also play a key role depending on the wine.

References:

[1] Marchal et al. J. Agric. Food Chem., 2002, 50, 1420
[2] Martí-Raga et al. J. Agric. Food Chem., 2016, 96, 4962
[3] Apolinar-Valiente et al. J. Agric. Food Chem., Under Review
[4] Apolinar-Valiente et al. Food Hydrocoll., 2019, 89, 864

 

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Rafael Apolinar-Valiente (1), Pascale Williams (2), Thomas Salmon (3), Michaël Nigen (1), Christian Sanchez (1), Richard Marchal (3), Thierry Doco (2)

(1) UMR 1208 Ingénierie des Agropolymères et Technologies Emergentes, Université de Montpellier2, CIRAD, Montpellier SupAgro, INRA, Montpellier
(2) UMR 1083 Sciences Pour l’OEnologie, Montpellier SupAgro, INRA, Université de Montpellier2, Montpellier, France
(3) Laboratoire d’Oenologie et Chimie Appliquée, Université de Reims, Reims, France

Contact the author

Keywords

Acacia senegal gum, sparkling wine, Ionic Exchange Chromatography , foamability

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

1H-NMR-based Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

The aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through untargeted and targeted 1H-NMR metabolomics. One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz. The spectra were recorded by applying the NOESYGPPS1D pulse sequency, to achieve water and ethanol signals suppression. No modification of the pH was performed to avoid any chemical alteration of the matrix. The generation of input variables for untargeted analysis was done via bucketing the spectra. The resulting dataset was preprocessed prior to perform unsupervised PCA, by means of MetaboAnalyst web-based tool suite. The identification of compounds for the targeted analysis was performed by comparison to pure compounds spectra by means of SMA plug-in of MNova 14.2.3 software. The dataset containing the concentrations (%) of identified compounds was subjected to one-way analysis of variance (ANOVA) to highlight significant differences among the wines. The untargeted analysis, carried out through the PCA, revealed a clear differentiation among the wines. The fragments of the spectra contributing mostly to the separation were attributed to flavonoids, aroma compounds and amino acids. The targeted analysis leaded to the identification of 68 compounds, whose concentrations were significant different among the wines. The results were related to soils physical-chemical analysis and showed that: 1) high concentrations of flavan-3-ols and flavonols are correlated with high clay content in soils; 2) high concentrations of anthocyanins, amino acids, and aroma compounds are correlated with neutral and moderately alkaline soil pH; 3) low concentrations of flavonoids and aroma compounds are correlated with high soil organic matter content and acidic pH. The 1H-NMR metabolomic analysis proved to be an excellent tool to discriminate between wines originating from grapes grown on different soil types and revealed that soils in the Mediterranean area exert a strong impact on the chemical composition of the wines.