OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Abstract

Foam is a key aspect of quality of sparkling wines. Bentonite is usually added to the wine to prevent protein haze, but reducing its foamability [1]. New skills are searching to avoid this undesirable event [2]. Acacia senegal gum (Asen) is an exudate from Acacia trees, which can be used to stabilize red wine color. Asen can be fractionated, and the most widely used method is Hydrophobic Interaction Chromatography (HIC) to obtain low (HIC-F1), medium (HIC-F2) and high (HIC-F3) molar mass fractions. The effect of these fractions on the foamability of bentonite-treated wines was studied, showing positive or negative effects depending on the fraction and the wine [3].

Asen can also be fractionated by Ion Exchange Chromatography (IEC) giving a high (IEC-F1) and low (IEC-F2) molar mass fractions [4]. A synthetic wine (SYWI) was prepared (12 % v/v ethanol, 3 g·L-1 of tartaric acid). 8 base wines from Spain (3) and France (5) were made by the traditional white winemaking method. They were treated with bentonite (20 g·hL-1), stirred gently for a few hours, kept in cold storage (10 days, 4 °C), racked and filtered (1 μm). IEC-fractions were added to SYWI (60 g·hL-1) and to wines (30 and 10 g·hL-1). The foaming parameters were compared by shake test and by a classical gas-sparging method (Mosalux), being the qualitative aspect of foam also observed.

In SYWI, IEC-F1 improves the foamability during the total shake test. Both fractions enhance its Maximum Foam Height (HM) and the Foam Stability Height at 5 minutes (HS) measured by Mosalux. IEC-F1 provides less compact foam with larger bubble. In Spanish wines, IEC-F1 increases the foamability during the total shake test. IEC-F1 also improves it in French wines, but weaker and differently depending on the wine. The foamability is punctually enhanced by IEC-F2 in some wines, but it is greatly decreased in 1 French wine. The dose reduction decreases the improving impact of IEC-F1 on the foamability of the French selected wine but not in the Spanish selected wine. IEC-F1 increases HM and HS in both selected wines, whereas IEC-F2 improves HS only in the Spanish selected wine.

Concluding, the addition of IEC-F1 increases foamability for all the studied wines, but very differently depending on the wine. IEC-F2 addition shows positive, neutral or even negative effects depending on the wine. Dose of IEC-F1 may also play a key role depending on the wine.

References:

[1] Marchal et al. J. Agric. Food Chem., 2002, 50, 1420
[2] Martí-Raga et al. J. Agric. Food Chem., 2016, 96, 4962
[3] Apolinar-Valiente et al. J. Agric. Food Chem., Under Review
[4] Apolinar-Valiente et al. Food Hydrocoll., 2019, 89, 864

 

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Rafael Apolinar-Valiente (1), Pascale Williams (2), Thomas Salmon (3), Michaël Nigen (1), Christian Sanchez (1), Richard Marchal (3), Thierry Doco (2)

(1) UMR 1208 Ingénierie des Agropolymères et Technologies Emergentes, Université de Montpellier2, CIRAD, Montpellier SupAgro, INRA, Montpellier
(2) UMR 1083 Sciences Pour l’OEnologie, Montpellier SupAgro, INRA, Université de Montpellier2, Montpellier, France
(3) Laboratoire d’Oenologie et Chimie Appliquée, Université de Reims, Reims, France

Contact the author

Keywords

Acacia senegal gum, sparkling wine, Ionic Exchange Chromatography , foamability

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Water deficit differentially impacts the performances and the accumulation of grape metabolites of new varieties tolerant to fungi

The use of resistant varieties is a long-term but promising solution to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are now releasing a range of new hybrids performing well regarding fungi susceptibility and producing good quality wines. Unfortunately, insufficient attention is paid by the breeders to the adaptation of these varieties to climatic changes, notably to the increased climatic demand and water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD. This study aimed to characterize the different drought-strategies adopted by 6 new resistant varieties selected by INRAE in comparison to Syrah. To allow the assessment of long-term impacts of WD, field-grown vines were exposed to contrasted WD from 2018 to 2021 under a semi-arid Mediterranean climate. A gradient of WD was applied in the field and controlled through plant measurements at the single plant level. Grape development was non-destructively monitored to determine the arrest of berry phloem unloading. The impacts of WD on berry composition, including water, primary metabolites (sugars, organic acids), secondary metabolites (anthocyanins, thiols precursors) and main cations contents, were assessed at this specific stage. Results showed different varietal responses during the year and inter-annual acclimation in terms of plant water use efficiency, biomass accumulation, as well as yield components and berry composition. WD differentially reduced the accumulation of primary metabolites at plant and berry levels, but it little changed their concentrations in the fruits at the ripe stage. Moreover, WD differentially impacted the accumulation of secondary metabolites and major cations between the varieties. In the talk, we’ll present the main results regarding the WD impacts on fruit metabolites and enlarge the reflection about the practical assessment of the grapevine acclimation to WD.

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.

1H-NMR-based Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

The aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through untargeted and targeted 1H-NMR metabolomics. One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz. The spectra were recorded by applying the NOESYGPPS1D pulse sequency, to achieve water and ethanol signals suppression. No modification of the pH was performed to avoid any chemical alteration of the matrix. The generation of input variables for untargeted analysis was done via bucketing the spectra. The resulting dataset was preprocessed prior to perform unsupervised PCA, by means of MetaboAnalyst web-based tool suite. The identification of compounds for the targeted analysis was performed by comparison to pure compounds spectra by means of SMA plug-in of MNova 14.2.3 software. The dataset containing the concentrations (%) of identified compounds was subjected to one-way analysis of variance (ANOVA) to highlight significant differences among the wines. The untargeted analysis, carried out through the PCA, revealed a clear differentiation among the wines. The fragments of the spectra contributing mostly to the separation were attributed to flavonoids, aroma compounds and amino acids. The targeted analysis leaded to the identification of 68 compounds, whose concentrations were significant different among the wines. The results were related to soils physical-chemical analysis and showed that: 1) high concentrations of flavan-3-ols and flavonols are correlated with high clay content in soils; 2) high concentrations of anthocyanins, amino acids, and aroma compounds are correlated with neutral and moderately alkaline soil pH; 3) low concentrations of flavonoids and aroma compounds are correlated with high soil organic matter content and acidic pH. The 1H-NMR metabolomic analysis proved to be an excellent tool to discriminate between wines originating from grapes grown on different soil types and revealed that soils in the Mediterranean area exert a strong impact on the chemical composition of the wines.