OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Abstract

Foam is a key aspect of quality of sparkling wines. Bentonite is usually added to the wine to prevent protein haze, but reducing its foamability [1]. New skills are searching to avoid this undesirable event [2]. Acacia senegal gum (Asen) is an exudate from Acacia trees, which can be used to stabilize red wine color. Asen can be fractionated, and the most widely used method is Hydrophobic Interaction Chromatography (HIC) to obtain low (HIC-F1), medium (HIC-F2) and high (HIC-F3) molar mass fractions. The effect of these fractions on the foamability of bentonite-treated wines was studied, showing positive or negative effects depending on the fraction and the wine [3].

Asen can also be fractionated by Ion Exchange Chromatography (IEC) giving a high (IEC-F1) and low (IEC-F2) molar mass fractions [4]. A synthetic wine (SYWI) was prepared (12 % v/v ethanol, 3 g·L-1 of tartaric acid). 8 base wines from Spain (3) and France (5) were made by the traditional white winemaking method. They were treated with bentonite (20 g·hL-1), stirred gently for a few hours, kept in cold storage (10 days, 4 °C), racked and filtered (1 μm). IEC-fractions were added to SYWI (60 g·hL-1) and to wines (30 and 10 g·hL-1). The foaming parameters were compared by shake test and by a classical gas-sparging method (Mosalux), being the qualitative aspect of foam also observed.

In SYWI, IEC-F1 improves the foamability during the total shake test. Both fractions enhance its Maximum Foam Height (HM) and the Foam Stability Height at 5 minutes (HS) measured by Mosalux. IEC-F1 provides less compact foam with larger bubble. In Spanish wines, IEC-F1 increases the foamability during the total shake test. IEC-F1 also improves it in French wines, but weaker and differently depending on the wine. The foamability is punctually enhanced by IEC-F2 in some wines, but it is greatly decreased in 1 French wine. The dose reduction decreases the improving impact of IEC-F1 on the foamability of the French selected wine but not in the Spanish selected wine. IEC-F1 increases HM and HS in both selected wines, whereas IEC-F2 improves HS only in the Spanish selected wine.

Concluding, the addition of IEC-F1 increases foamability for all the studied wines, but very differently depending on the wine. IEC-F2 addition shows positive, neutral or even negative effects depending on the wine. Dose of IEC-F1 may also play a key role depending on the wine.

References:

[1] Marchal et al. J. Agric. Food Chem., 2002, 50, 1420
[2] Martí-Raga et al. J. Agric. Food Chem., 2016, 96, 4962
[3] Apolinar-Valiente et al. J. Agric. Food Chem., Under Review
[4] Apolinar-Valiente et al. Food Hydrocoll., 2019, 89, 864

 

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Rafael Apolinar-Valiente (1), Pascale Williams (2), Thomas Salmon (3), Michaël Nigen (1), Christian Sanchez (1), Richard Marchal (3), Thierry Doco (2)

(1) UMR 1208 Ingénierie des Agropolymères et Technologies Emergentes, Université de Montpellier2, CIRAD, Montpellier SupAgro, INRA, Montpellier
(2) UMR 1083 Sciences Pour l’OEnologie, Montpellier SupAgro, INRA, Université de Montpellier2, Montpellier, France
(3) Laboratoire d’Oenologie et Chimie Appliquée, Université de Reims, Reims, France

Contact the author

Keywords

Acacia senegal gum, sparkling wine, Ionic Exchange Chromatography , foamability

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

The use of rootstock as a lever in the face of climate change and dieback of vineyard

As viticulture faces challenges such as climate change or vineyard dieback, the choice of the variety and rootstock becomes more and more crucial. To study rootstock levers in the Bordeaux region, a parcel of Cabernet Sauvignon (CS) was planted with four rootstocks in 2014. Twenty repetitions of each of the following four rootstocks were set up: 101-14 MGt, Nemadex AB, 420A MGt and Gravesac. The number of bunches, yields and pruning weights of the vine shoots were measured individually on 240 vines from 2017 to 2021. Since 2020, nitrogen status assessed by assimilable nitrogen level, hydric status assessed by δ13C and berry maturity were measured on 80 samples taken from 20 repetitions of the four rootstocks. A lower yield was measured for CS grafted onto Nemadex AB due to the lower number of bunches and the lower weight of berries. The differences between the other three rootstocks are small, but CS grafted onto 420A MGt was the most productive. The CS grafted onto Nemadex AB had the lowest pruning weight while 101-14 MGt had the highest. In 2020, δ13C showed a more moderate water stress with 101-14 MGt and 420A MGt than with Nemadex AB. Surprisingly, the Gravesac was under more stress than the 101-14 MGt. The nitrogen status in the berries was better for Nemadex AB but this was perhaps due to the significantly lower weight of the berries.Rootstock 101-14 MGt attained the highest accumulation of sugars in the berries while 420A MGt allows to preserve higher acidity. The parcel is still young which may explain some of the results. These measures must therefore be continued over the next several years to fully assess the effects of these rootstocks on the development of the vines and the quality of the production under new climatic conditions.

Impact of long term agroecological and conventional practices on subsurface soil microbiota in Macabeu and Xarel·lo vineyards

There is a growing trend on the transition from conventional to agroecological management of vineyards. However, the impact of practices, such as reduced-tillage, organic fertilization and cover crops, is not well-understood regarding the soil microbial diversity, and its relationship with the soil physicochemical properties in the subsurface depth near the rooting zone. Soil bacterial diversity is an important contributor towards plant health, productivity and response to environmental stresses. A field experiment was conducted by sampling subsurface soil bacterial community (NGS and qPCR) near to the root zone of Macabeu and Xarel·lo vineyards, located at the Penedes. 3 organic (ECO) and 3 conventional (CON) vineyards, with more than 10 years of respective management were sampled (n=5 each plot). ECO practices did not affect bacterial and fungal abundance but increased significantly the ammonium oxidizing bacteria and alpha-diversity (Inv.Simpson). Interestingly beta-diversity was significantly affected by the management strategy. ANOSIM-tests revealed a significative effect of the management (ecological vs conventional) and plot, on the soil microbial structure (ASV abundance). Main phyla depicted were Proteobacteria, Actinobacteria and Acidobacteria, whose relative abundances were not affected by the management. EdgeR assay revealed a significant increase of Cyanobacteria and decrease of Gemmatimonadetes and Firmicutes phyla in ECO. Interestingly, the grapevine variety was not correlated with the soil microbial community structure. Mantel-test revealed an important correlation (Spearman) of some physicochemical parameters with the soil microbiota structure, in order of importance: texture, EC, pH Ca/Mg, Mg/P, K+, Mg2+, Ca2+, SO42-, and OM. N-NH4 and NTK, which were higher in the ECO managed soils, did not correlated significantly with the soil microbiome population. The results revealed the importance of combining a deep physicochemical characterization of each replicate with the microbial diversity assessment to gain better insights on the relationship between soil microbiome and vineyard management.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Water deficit differentially impacts the performances and the accumulation of grape metabolites of new varieties tolerant to fungi

The use of resistant varieties is a long-term but promising solution to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are now releasing a range of new hybrids performing well regarding fungi susceptibility and producing good quality wines. Unfortunately, insufficient attention is paid by the breeders to the adaptation of these varieties to climatic changes, notably to the increased climatic demand and water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD. This study aimed to characterize the different drought-strategies adopted by 6 new resistant varieties selected by INRAE in comparison to Syrah. To allow the assessment of long-term impacts of WD, field-grown vines were exposed to contrasted WD from 2018 to 2021 under a semi-arid Mediterranean climate. A gradient of WD was applied in the field and controlled through plant measurements at the single plant level. Grape development was non-destructively monitored to determine the arrest of berry phloem unloading. The impacts of WD on berry composition, including water, primary metabolites (sugars, organic acids), secondary metabolites (anthocyanins, thiols precursors) and main cations contents, were assessed at this specific stage. Results showed different varietal responses during the year and inter-annual acclimation in terms of plant water use efficiency, biomass accumulation, as well as yield components and berry composition. WD differentially reduced the accumulation of primary metabolites at plant and berry levels, but it little changed their concentrations in the fruits at the ripe stage. Moreover, WD differentially impacted the accumulation of secondary metabolites and major cations between the varieties. In the talk, we’ll present the main results regarding the WD impacts on fruit metabolites and enlarge the reflection about the practical assessment of the grapevine acclimation to WD.