OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Foamability of bentonite treated wines: impact of new acacia gum fractions obtained by ionic exchange chromatography (IEC)

Abstract

Foam is a key aspect of quality of sparkling wines. Bentonite is usually added to the wine to prevent protein haze, but reducing its foamability [1]. New skills are searching to avoid this undesirable event [2]. Acacia senegal gum (Asen) is an exudate from Acacia trees, which can be used to stabilize red wine color. Asen can be fractionated, and the most widely used method is Hydrophobic Interaction Chromatography (HIC) to obtain low (HIC-F1), medium (HIC-F2) and high (HIC-F3) molar mass fractions. The effect of these fractions on the foamability of bentonite-treated wines was studied, showing positive or negative effects depending on the fraction and the wine [3].

Asen can also be fractionated by Ion Exchange Chromatography (IEC) giving a high (IEC-F1) and low (IEC-F2) molar mass fractions [4]. A synthetic wine (SYWI) was prepared (12 % v/v ethanol, 3 g·L-1 of tartaric acid). 8 base wines from Spain (3) and France (5) were made by the traditional white winemaking method. They were treated with bentonite (20 g·hL-1), stirred gently for a few hours, kept in cold storage (10 days, 4 °C), racked and filtered (1 μm). IEC-fractions were added to SYWI (60 g·hL-1) and to wines (30 and 10 g·hL-1). The foaming parameters were compared by shake test and by a classical gas-sparging method (Mosalux), being the qualitative aspect of foam also observed.

In SYWI, IEC-F1 improves the foamability during the total shake test. Both fractions enhance its Maximum Foam Height (HM) and the Foam Stability Height at 5 minutes (HS) measured by Mosalux. IEC-F1 provides less compact foam with larger bubble. In Spanish wines, IEC-F1 increases the foamability during the total shake test. IEC-F1 also improves it in French wines, but weaker and differently depending on the wine. The foamability is punctually enhanced by IEC-F2 in some wines, but it is greatly decreased in 1 French wine. The dose reduction decreases the improving impact of IEC-F1 on the foamability of the French selected wine but not in the Spanish selected wine. IEC-F1 increases HM and HS in both selected wines, whereas IEC-F2 improves HS only in the Spanish selected wine.

Concluding, the addition of IEC-F1 increases foamability for all the studied wines, but very differently depending on the wine. IEC-F2 addition shows positive, neutral or even negative effects depending on the wine. Dose of IEC-F1 may also play a key role depending on the wine.

References:

[1] Marchal et al. J. Agric. Food Chem., 2002, 50, 1420
[2] Martí-Raga et al. J. Agric. Food Chem., 2016, 96, 4962
[3] Apolinar-Valiente et al. J. Agric. Food Chem., Under Review
[4] Apolinar-Valiente et al. Food Hydrocoll., 2019, 89, 864

 

DOI:

Publication date: June 10, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Rafael Apolinar-Valiente (1), Pascale Williams (2), Thomas Salmon (3), Michaël Nigen (1), Christian Sanchez (1), Richard Marchal (3), Thierry Doco (2)

(1) UMR 1208 Ingénierie des Agropolymères et Technologies Emergentes, Université de Montpellier2, CIRAD, Montpellier SupAgro, INRA, Montpellier
(2) UMR 1083 Sciences Pour l’OEnologie, Montpellier SupAgro, INRA, Université de Montpellier2, Montpellier, France
(3) Laboratoire d’Oenologie et Chimie Appliquée, Université de Reims, Reims, France

Contact the author

Keywords

Acacia senegal gum, sparkling wine, Ionic Exchange Chromatography , foamability

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Effects of organic mulches on the soil environment and yield of grapevine

Farming management practices aiming at conserving soil moisture have been developed in arid and semiarid-areas facing water scarcity problems. Organic mulching is an effective method to manipulate the crop-growing microclimate increasing crop yield by controlling soil temperature, and retaining soil moisture by reducing soil evaporation. In this sense, the effectiveness of different organic mulching materials (straw mulch and grapevine pruning debris) applied within the row of a vineyard was evaluated on the soil and on the vine in a Tempranillo vineyard located in La Rioja (Spain). Organic mulches were compared with a traditional bare soil management technique (based on the use of herbicides to avoid weed incidence). Mulching coverages favourably influenced the soil water retention throughout all the grapevine vegetative cycle. However, the soil-moisture variation was not the same under different mulching materials, being the straw mulch (SM) the one that retained more water in comparison with grapevine pruning debris (GPD) based-cover. The changes of soil moisture in the upper surface layer (0–10 cm) were highly dynamic, probably due to water vapour fluxes across the soil-atmospheric interface. However, both, SM and GPD reduced these fluctuations as compared with bare soils. A similar trend occurred with soil temperature. Both organic mulches altered soil temperature in comparison with bare soil by reducing soil temperature in summer and raising it in winter. Moreover, the same buffering effect for the temperature on the covered soil also remains in the deeper layers. To conclude, we could see that organic mulching had a positive impact on soil-moisture storage and soil temperature and the extent of this effect depends on the type of mulching materials. These changes led to higher rates of photosynthesis and stomatal conductivity compared to bare soils, also favouring crop growth and grape yields.

Protected Designation of Origin (D.P.O.) Valdepeñas: classification and map of soils

The objective of the work described here is the elaboration of a map of the different types of vineyard soils that to guide the famers in the choice of the most productive vine rootstocks and varieties. 90 vineyard soils profiles were analysed in the entire territory of the Origen Denominations of Valdepeñas. The sampling was carried out in 2018 (June to October) by making a sampling grid, followed by photointerpretation and control in the field. The studied soils can be grouped into 9 different soil types (according to FAO 2006 classification): Leptosols, Regosols, Fluvisols, Gleysols, Cambisols, Calcisols, Luvisols and Anthrosols. A map showing the soil distribution with different type of soils has been made with the ArcGIS program. Regarding to the choice of rootstock, Calcisoles are soils with a high active limestone content, so the rootstocks used in these soils must be resistant to this parameter; Luvisols are deep soils with high clay content, so they will support vigorous rootstocks. Because the cartographic units are composed of two or more subgroups, with are associated in variable proportions, 9 different soil associations have been established; Unit 1: Leptosols, Cambisols and Luvisols (80%, 15% and 5% respectively); Unit 2: Cambisols with Regosols and Luvisols (40%, 30% and 30% respectively); Unit 3: Cambisols and Gleysols with Regosols (40%, 40% and 20% respectively); Unit 4: Regosols with Cambisols, Leptosols and Calcisols (40%, 30%, 15% and 15% respectively); Unit 5: Cambisols, Leptosols, Calcisols and Regosols (25% each of them); Unit 6: Luvisols with Cambisol and Calcisols (80%, 10% and 10% respectively); Unit 7: Luvisols and Calcisols with Cambisols (40%, 40% and 20% respectively); Unit 8: Calcisols with, Cambisols and Luvisols (80%, 10% and 10% respectively); Unit 9: Anthrosols. These study allow to elaborate the first map of vineyard soils of this Protected Designation of Origin in Castilla-La Mancha.

The impact of leaf canopy management on eco-physiology, wood chemical properties and microbial communities in root, trunk and cordon of Riesling grapevines (Vitis vinifera L.)

In the last decades, climate change required already adaptation of vineyard management. Increase in temperature and unexpected weather events cause changes in all phenological stages requiring new management tools. For example, defoliation can be a useful tool to reduce the sugar content in the berries creating differences in the wine profiles. In a ten-year field experiment using Riesling (Vitis vinifera L, planted 1986, Geisenheim, Germany), various mechanical defoliation strategies and different intensities were trialed until 2016 before the vineyard was uprooted. Wood was sampled from the plant compartments root, trunk, cordon and shoot for analyses of physicochemical properties (e.g. lignin and element content, pH, diameter), nonstructural carbohydrates and the microbial communities. The aim of the study was to investigate the influence of reduced canopy leaf area on the sink-source allocation into different compartments and potential changes of the fungal and prokaryotic wood-inhabiting community using a metabarcoding approach. Severe summer pruning (SSP) of the canopy and mechanical defoliation (MDC) above the bunch zone decreased the leaf area by 50% compared to control (C). SSP reduced the photosynthetic capacity, which resulted in an altered source-sink allocation and carbohydrate storage. With lower leaf area, less carbohydrates are allocated. This for example resulted in a decreased trunk diameter. Further, it affected the composition of the grapevine wood microbiota. SSP and MDC management changed significantly the prokaryotic community composition in wood of the root samples, but had no effect in other compartments. In general, this study found strong compartment and less management effects of the microbial community composition and associated physicochemical properties. The highest microbial diversities were identified in the wood of the trunk, and several species were recorded the first time in grapevine.

Grape must quality and mesoclimatic variability in Fruška Gora wine-growing region, Serbia

The Fruška Gora mountain is a traditional wine-growing region in Serbia situated in the Pannonian Basin. Due to such a position, the vicinity of the Danube River and the presence of concave configuration, it is suitable for grape production. This paper provides analyses of spatial variations in meteorological parameters and grape juice quality within Fruška Gora wine region over three consecutive vintages (2018-2020). The examined period can be defined as warm with cool nights during September (AVG 18,9°C; GDD 1918°C; CI 12°CF) and with the presence of mesoclimatic variability. The East part of the study area was somewhat drier and hotter compared to other parts of the region. The analyses of grape must samples (190 in total) of five cultivars (Cabernet-Sauvignon, Merlot, Chardonnay, Sauvignon blanc and Grašac (Welschriesling)) commonly grown across the region (19 sites), were performed using Fourier Transform Infrared Technology (FTIR). Among all cultivars, Sauvignon blanc was harvested first in the East area (DOY=246±5, GDD at harvest=1552±74, 22.2±0.7 °Brix), while the latest harvest was recorded for Cabernet-Sauvignon in the West (DOY=283±5, GDD at harvest=1936±187, 23.4±1.0 °Brix ). Both the red and white cultivars had higher acidity and YAN in the grape must if the vines were grown in the North and East compared to South and West areas. According to PCA analysis, Grašac showed the lowest variation in grape must chemical composition. Thus, the results confirm that Grašac is the most stable cultivar in Fruška Gora. All monitored cultivars reached technological fruit ripeness by the end of the growing season. However, it was difficult to reach full ripeness of red cultivars, mostly beacuse of uncoupling of technolocical and phenolic ripeness. Thus, Cabernet-Sauvignon had higher variations in GDD sums at harvest compared to other cultivars, which probably increased variations in grape must quality.

Vineyards and clay minerals: multi-technique analytical approach and correlations with soil properties

Purpose of this research is to quantitatively assess the mineral component of vineyard soils, with particular attention to the mineralogical analysis of clays, which represent an element of high importance in the vineyard culture as well as in general agriculture. An X-ray diffraction (XRD) / thermogravimetric (TG) multi-technique analytical approach was developed, tested on soil samples taken from vineyards around the world. This codified analytical procedure was necessary to obtain precise qualitative and quantitative mineralogical data, globally comparable to distinguish the geopedological identity of the vineyards. Soil samples from vineyards of various locations were analysed, in very different geological conditions. The bulk-rock quantitative phase analysis (QPA) was obtained by the Rietveld method while the detailed composition of the clay-sized fraction was determined by modelling of the oriented X-ray diffraction patterns. The research provided a precise classification of the mineral component of soils, distinguishing the mineral phases of the clays and the so-called mixed-layer clay minerals. We found that the content in mixed layers can be directly correlated with the water retention and the cation exchange capacity ​​of the soil, while the presence of other clayey minerals and phyllosilicates in this research did not affect this CEC parameter, which codes the fertility level of the soils. The study demonstrates that terroir, in particular soils formed in complex or very different geological conditions, can only be effectively interpreted by properly analysing its mineral phases, in particular the mixed-layer clay component. These are characteristic abiotic ecological indicators, which may have specific eco-physiological influences on the plant.