GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Application of a fluorescence-based method to evaluate the ripening process and quality of Pinot Blanc grape

Application of a fluorescence-based method to evaluate the ripening process and quality of Pinot Blanc grape

Abstract

Context and purpose of the study – The chemical composition of grape berries at harvest is one of the most important factors that should be considered to produce high quality wines. Among the different chemical classes which characterize the grape juice, the polyphenolic compound, such as flavonoids, contribute to the final taste and color of wines. Recently, an innovative non-destructive method, based on chlorophyll fluorescence, was developed to estimate the phenolic maturity of red grape varieties through the evaluation of anthocyanins accumulated in the berry skin. To date, only few data are available about the application of this method on white grape varieties. The aim of the present study was to evaluate the quality and the ripening process of Pinot blanc grape by a non-destructive fluorescence-based sensor.

Material and methods – The study was performed on two vineyards of cv. Pinot blanc located in the Adige Valley (South Tyrol, Bolzano), in two consecutive vintages. The vineyard differed in the row orientation, east-west or north-south, and then on the sun light exposure of the grape-bunches. The grape phenolic maturity was assessed on intact berries by six measurements from bunch closure to harvest time. In each vineyard, 25 grape-bunches per row sides were flashed by Multiplex® 3.6 (Force-A, Orsay, France), for a total of 3 rows and 150 grape-bunches/measurement. The instrument indices of chlorophyll (SFR_R) and flavonols (FLAV_UV) were considered. Standard grape maturity tests were performed to assess total soluble solids (TSS) and total acidity content of the grape juice by spectroscopic method. At maturity the grapes were processed with a standard vinification protocol for white wines. Total polyphenolic content of wines was determined by a spectrophotometric analysis.

Results – A linear decrease of SFR_R index in the berry-skin during the grape ripening period was recorded. Interestingly, SFR_R values negative correlated with the TTS accumulation in Pinot blanc berries. On the other side, positive correlations between SFR_R and titratable acidity, malic acid and tartaric acid content, were observed. The FLAV_UV index showed an increasing linear trend during the grape ripening period. At harvest, significant difference in FLAV_UV index between the two vineyards was observed. Looking more deeply inside the data, the berry-skin FLAV_UV index significantly differed among the four sun-light expositions, with greater values recorded for the grape-bunches located in south and east sides of the vineyard rows. These results are in accordance with the available literature on the role flavonols as sun-burn protection compounds. Interestingly, the total polyphenolic content of the produced wines showed a positive correlation with the final FLAV_UV values measured in the berry-skin. In conclusion, the Multiplex® indices could improve precision viticulture strategies, such as the implementation of precision harvest practices. Indeed, SFR_R index could be used to indirectly evaluate the whole ripening process of white grapes in term of grape sugar content and acidity, while FLAV_UV could provide useful indications to winemakers about taste of final product. Future studies will be necessary to better correlate the berry-skin FLAV_UV values and the flavours of white wine.

DOI:

Publication date: September 29, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Selena TOMADA1*, Florian PICHLER1, Julia MARTINELLI1, Giovanni AGATI2, Valentina LAZAZZARA3, Martin ZEJFART4, Fenja HINZ3, Ulrich PEDRI4, Peter ROBATSCHER3, Florian HAAS1

1 Department of Viticulture, Laimburg Research Centre, BZ, Italy
2 Istituto di Fisica Applicata ‘Nello Carrara’, CNR, FI, Italy
3 Laboratory for Flavours and Metabolites, Laimburg Research Centre, BZ, Italy
4 Department of Enology, Laimburg Research Centre, BZ, Italy

Contact the author

Keywords

chlorophyll, flavonols, grape, Multiplex®, quality, Pinot blanc.

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

Impact of long term agroecological and conventional practices on subsurface soil microbiota in Macabeu and Xarel·lo vineyards

There is a growing trend on the transition from conventional to agroecological management of vineyards. However, the impact of practices, such as reduced-tillage, organic fertilization and cover crops, is not well-understood regarding the soil microbial diversity, and its relationship with the soil physicochemical properties in the subsurface depth near the rooting zone. Soil bacterial diversity is an important contributor towards plant health, productivity and response to environmental stresses. A field experiment was conducted by sampling subsurface soil bacterial community (NGS and qPCR) near to the root zone of Macabeu and Xarel·lo vineyards, located at the Penedes. 3 organic (ECO) and 3 conventional (CON) vineyards, with more than 10 years of respective management were sampled (n=5 each plot). ECO practices did not affect bacterial and fungal abundance but increased significantly the ammonium oxidizing bacteria and alpha-diversity (Inv.Simpson). Interestingly beta-diversity was significantly affected by the management strategy. ANOSIM-tests revealed a significative effect of the management (ecological vs conventional) and plot, on the soil microbial structure (ASV abundance). Main phyla depicted were Proteobacteria, Actinobacteria and Acidobacteria, whose relative abundances were not affected by the management. EdgeR assay revealed a significant increase of Cyanobacteria and decrease of Gemmatimonadetes and Firmicutes phyla in ECO. Interestingly, the grapevine variety was not correlated with the soil microbial community structure. Mantel-test revealed an important correlation (Spearman) of some physicochemical parameters with the soil microbiota structure, in order of importance: texture, EC, pH Ca/Mg, Mg/P, K+, Mg2+, Ca2+, SO42-, and OM. N-NH4 and NTK, which were higher in the ECO managed soils, did not correlated significantly with the soil microbiome population. The results revealed the importance of combining a deep physicochemical characterization of each replicate with the microbial diversity assessment to gain better insights on the relationship between soil microbiome and vineyard management.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

First step in the preparation of a soil map of the Protected Designation of Origin Valdepeñas (Central, Spain)

This work is a first step to make a map of vineyard soils. The characterization of the soils of the Protected Designation of Origin (D.P.O.) Valdepeñas will allow to group the studied profiles according to their physico-chemical characteristics and the concentrations of most relevant chemical elements. 90 soil profiles were analysed throughout the territory and the soils were sampled and described according to FAO (2006) and classified according to and Soil Taxonomy (2014). All samples were air dried, sieved and some physico-chemical parameters were determined following standard protocols. Also, major and trace elements were analysed by X-ray fluorescence. The statistically study was made using the SPSS program. Trend maps were made using the ArcGIS program. The studied soils have the following average properties: pH, 8.3; electrical conductivity, 0,20 dS/m (low); clay, 18.8% (medium) and CaCO3, 17.1% (high). In the study for the major elements. The major elements of these soils are Si, followed by Ca and Al, with an average content of 203.7 g/kg, 105.5 g/kg and 74.0 g/kg respectively. On the other hand, 27 trace elements have been studied. Of all of them, it can be highlighted the average values of Ba (361.8 mg/kg), Sr (129.3 mg/kg), Rb (83.4 mg/kg), V (74.2 mg/kg) and Ce (70.6 mg/kg). Ba, V and Ce values are higher and the values of Sr and Rb are lower to those found in the literature. The discriminant analysis shows a percentage of grouping of 91%. The content of chemical elements together with the physico-chemical characteristics allows grouping the soils in 4 group according to their order in the classification to Soil Taxonomy; due to the importance of the Calcisols in Castilla-La Mancha, it has been decided to establish them as their own group even if they do not appear in Soil Taxonomy classification.