GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Application of a fluorescence-based method to evaluate the ripening process and quality of Pinot Blanc grape

Application of a fluorescence-based method to evaluate the ripening process and quality of Pinot Blanc grape

Abstract

Context and purpose of the study – The chemical composition of grape berries at harvest is one of the most important factors that should be considered to produce high quality wines. Among the different chemical classes which characterize the grape juice, the polyphenolic compound, such as flavonoids, contribute to the final taste and color of wines. Recently, an innovative non-destructive method, based on chlorophyll fluorescence, was developed to estimate the phenolic maturity of red grape varieties through the evaluation of anthocyanins accumulated in the berry skin. To date, only few data are available about the application of this method on white grape varieties. The aim of the present study was to evaluate the quality and the ripening process of Pinot blanc grape by a non-destructive fluorescence-based sensor.

Material and methods – The study was performed on two vineyards of cv. Pinot blanc located in the Adige Valley (South Tyrol, Bolzano), in two consecutive vintages. The vineyard differed in the row orientation, east-west or north-south, and then on the sun light exposure of the grape-bunches. The grape phenolic maturity was assessed on intact berries by six measurements from bunch closure to harvest time. In each vineyard, 25 grape-bunches per row sides were flashed by Multiplex® 3.6 (Force-A, Orsay, France), for a total of 3 rows and 150 grape-bunches/measurement. The instrument indices of chlorophyll (SFR_R) and flavonols (FLAV_UV) were considered. Standard grape maturity tests were performed to assess total soluble solids (TSS) and total acidity content of the grape juice by spectroscopic method. At maturity the grapes were processed with a standard vinification protocol for white wines. Total polyphenolic content of wines was determined by a spectrophotometric analysis.

Results – A linear decrease of SFR_R index in the berry-skin during the grape ripening period was recorded. Interestingly, SFR_R values negative correlated with the TTS accumulation in Pinot blanc berries. On the other side, positive correlations between SFR_R and titratable acidity, malic acid and tartaric acid content, were observed. The FLAV_UV index showed an increasing linear trend during the grape ripening period. At harvest, significant difference in FLAV_UV index between the two vineyards was observed. Looking more deeply inside the data, the berry-skin FLAV_UV index significantly differed among the four sun-light expositions, with greater values recorded for the grape-bunches located in south and east sides of the vineyard rows. These results are in accordance with the available literature on the role flavonols as sun-burn protection compounds. Interestingly, the total polyphenolic content of the produced wines showed a positive correlation with the final FLAV_UV values measured in the berry-skin. In conclusion, the Multiplex® indices could improve precision viticulture strategies, such as the implementation of precision harvest practices. Indeed, SFR_R index could be used to indirectly evaluate the whole ripening process of white grapes in term of grape sugar content and acidity, while FLAV_UV could provide useful indications to winemakers about taste of final product. Future studies will be necessary to better correlate the berry-skin FLAV_UV values and the flavours of white wine.

DOI:

Publication date: September 29, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Selena TOMADA1*, Florian PICHLER1, Julia MARTINELLI1, Giovanni AGATI2, Valentina LAZAZZARA3, Martin ZEJFART4, Fenja HINZ3, Ulrich PEDRI4, Peter ROBATSCHER3, Florian HAAS1

1 Department of Viticulture, Laimburg Research Centre, BZ, Italy
2 Istituto di Fisica Applicata ‘Nello Carrara’, CNR, FI, Italy
3 Laboratory for Flavours and Metabolites, Laimburg Research Centre, BZ, Italy
4 Department of Enology, Laimburg Research Centre, BZ, Italy

Contact the author

Keywords

chlorophyll, flavonols, grape, Multiplex®, quality, Pinot blanc.

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

The modification of cultural practices in grapevine cv. Syrah, does it modify the characteristics of the musts?

The work shows the results of a year of experimentation (2020) in a Syrah variety vineyard in La Roda (Castilla-La Mancha, Spain). The trial approach was on a randomized block design with two factors: Irrigation (I) and Pruning (P).
Irrigation schedules were adjusted to apply amounts close to 1,500 m3/ha. With this provision, 2 different irrigation treatments were proposed: I1) Start of irrigation from pea-sized grape to post-harvest (providing at least 20 % of the total amount of irrigation water to be provided post-harvest); I2) Start of irrigation from pea-sized grape to harvest (usual irrigation practice in the study area). Pruning was proposed with two treatments, one at the end of January (P1), which is pruning on a conventional date; and P2) pruning carried out at the beginning of budding. In total, 4 repetitions were designed with 4 elementary plots, each one of them representing one of the proposed treatments (I1P1; I1P2; I2P1; I2P2). In total, 16 plots were worked on and each elementary plot consisted of 30 strains, distributed in 3 lines.
The productive response was evaluated with the yield results of the harvest harvested at 23 ºBrix. The qualitative response was measured in the musts through the indices of technological (acidity, pH and potassium) and phenolic maturity and aromatic compounds in free and glycosylated fractions. The treatments tested had, in general, an effect on the different variables analyzed.

Terroir analysis and its complexity

Terroir is not only a geographical site, but it is a more complex concept able to express the “collective knowledge of the interactions” between the environment and the vines mediated through human action and “providing distinctive characteristics” to the final product (OIV 2010). It is often treated and accepted as a “black box”, in which the relationships between wine and its origin have not been clearly explained. Nevertheless, it is well known that terroir expression is strongly dependent on the physical environment, and in particular on the interaction between soil-plant and atmosphere system, which influences the grapevine responses, grapes composition and wine quality. The Terroir studying and mapping are based on viticultural zoning procedures, obtained with different levels of know-how, at different spatial and temporal scales, empiricism and complexity in the description of involved bio-physical processes, and integrating or not the multidisciplinary nature of the terroir. The scientific understanding of the mechanisms ruling both the vineyard variability and the quality of grapes is one of the most important scientific focuses of terroir research. In fact, this know-how is crucial for supporting the analysis of climate change impacts on terroir resilience, identifying new promised lands for viticulture, and driving vineyard management toward a target oenological goal. In this contribution, an overview of the last findings in terroir studies and approaches will be shown with special attention to the terroir resilience analysis to climate change, facing the use and abuse of terroir concept and new technology able to support it and identifying the terroir zones.