terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

Abstract

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane. Chitin, the major component of fungal cell walls, is well known to elicit plant immune responses. In Vitis vinifera, knowledge about the perception and immune responses triggered by chitin oligomers is now better understood [1]. Among the LysM receptor-like kinases (LysM-RLKs) family of PRRs, which includes 16 members in Vitis vinifera, three of them (VvLYK1-1, VvLYK1-2 and VvLYK5-1) are involved in the perception of chitin oligomers to trigger plant immune responses [1,2]. Recently, another member of this family, named VvLYK6, was identified in grapevine as being overexpressed following a Botrytis cinerea infection. We characterized the role of VvLYK6 in plant immunity by overexpressing it in Arabidopsis thaliana and grapevine. Surprisingly, overexpression of VvLYK6 decreased chitin-induced MAPK activation, defense gene expression, callose deposition and increased plant sensitivity to fungal infections. According to these results, VvLYK6 negatively regulates chitin-induced defense reactions in grapevine and could be considered as a susceptibility gene in the context of fungal infections.

Acknowledgements: We acknowledge Institut Carnot Plant2Pro for funding the VitiLYKs project and DimaCell platform (Dijon, France) for the confocal microscopy.

References:
1) Roudaire T. et al. (2023) The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYL1-1. Front. Plant Sci. 14:1130782 DOI 10.3389/fpls.2023.1130782

2) Brulé D. et al. (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnol. J. 17, 812–825. DOI 10.1111/pbi.13017

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Villette J.1*, Marzari T.1, Roudaire T.1, Klinguer A.1, Leborgne-Castel N.1, Héloir M-C.1, Poinssot B.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.

Contact the author*

Keywords

grapevine, plant defense, LysM receptor-like kinase, defense inhibition

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.

Read More

Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Grapevine cultivars are vegetatively propagated to preserve their varietal characteristics. However, spontaneous somatic variations that occur and are maintained during cycles of vegetative growth offer opportunities for the natural improvement of traditional grape cultivars. One advantageous trait for winegrowing is reduced bunch compactness, which decreases the susceptibility to pests and fungal diseases and favor an even berry ripening.

Read More

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...
Read More

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.

Read More

INTEGRAPE guidelines and tools: an effort of COST Action CA17111

INTEGRAPE was a European interdisciplinary network for “data integration to maximize the power of omics for grapevine improvement” (CA17111, https://integrape.eu/), funded by the European COST Association from September 2018 to 2022. This Action successfully developed guidelines and tools for data management and promoted the best practices in grapevine omics studies with a holistic future vision of: “Imagine having all data on grapevine accessible in a single place”.

Read More