terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

Abstract

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane. Chitin, the major component of fungal cell walls, is well known to elicit plant immune responses. In Vitis vinifera, knowledge about the perception and immune responses triggered by chitin oligomers is now better understood [1]. Among the LysM receptor-like kinases (LysM-RLKs) family of PRRs, which includes 16 members in Vitis vinifera, three of them (VvLYK1-1, VvLYK1-2 and VvLYK5-1) are involved in the perception of chitin oligomers to trigger plant immune responses [1,2]. Recently, another member of this family, named VvLYK6, was identified in grapevine as being overexpressed following a Botrytis cinerea infection. We characterized the role of VvLYK6 in plant immunity by overexpressing it in Arabidopsis thaliana and grapevine. Surprisingly, overexpression of VvLYK6 decreased chitin-induced MAPK activation, defense gene expression, callose deposition and increased plant sensitivity to fungal infections. According to these results, VvLYK6 negatively regulates chitin-induced defense reactions in grapevine and could be considered as a susceptibility gene in the context of fungal infections.

Acknowledgements: We acknowledge Institut Carnot Plant2Pro for funding the VitiLYKs project and DimaCell platform (Dijon, France) for the confocal microscopy.

References:
1) Roudaire T. et al. (2023) The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYL1-1. Front. Plant Sci. 14:1130782 DOI 10.3389/fpls.2023.1130782

2) Brulé D. et al. (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnol. J. 17, 812–825. DOI 10.1111/pbi.13017

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Villette J.1*, Marzari T.1, Roudaire T.1, Klinguer A.1, Leborgne-Castel N.1, Héloir M-C.1, Poinssot B.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.

Contact the author*

Keywords

grapevine, plant defense, LysM receptor-like kinase, defense inhibition

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries.

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).