terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

Abstract

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane. Chitin, the major component of fungal cell walls, is well known to elicit plant immune responses. In Vitis vinifera, knowledge about the perception and immune responses triggered by chitin oligomers is now better understood [1]. Among the LysM receptor-like kinases (LysM-RLKs) family of PRRs, which includes 16 members in Vitis vinifera, three of them (VvLYK1-1, VvLYK1-2 and VvLYK5-1) are involved in the perception of chitin oligomers to trigger plant immune responses [1,2]. Recently, another member of this family, named VvLYK6, was identified in grapevine as being overexpressed following a Botrytis cinerea infection. We characterized the role of VvLYK6 in plant immunity by overexpressing it in Arabidopsis thaliana and grapevine. Surprisingly, overexpression of VvLYK6 decreased chitin-induced MAPK activation, defense gene expression, callose deposition and increased plant sensitivity to fungal infections. According to these results, VvLYK6 negatively regulates chitin-induced defense reactions in grapevine and could be considered as a susceptibility gene in the context of fungal infections.

Acknowledgements: We acknowledge Institut Carnot Plant2Pro for funding the VitiLYKs project and DimaCell platform (Dijon, France) for the confocal microscopy.

References:
1) Roudaire T. et al. (2023) The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYL1-1. Front. Plant Sci. 14:1130782 DOI 10.3389/fpls.2023.1130782

2) Brulé D. et al. (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnol. J. 17, 812–825. DOI 10.1111/pbi.13017

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Villette J.1*, Marzari T.1, Roudaire T.1, Klinguer A.1, Leborgne-Castel N.1, Héloir M-C.1, Poinssot B.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.

Contact the author*

Keywords

grapevine, plant defense, LysM receptor-like kinase, defense inhibition

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock.

Read More

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...
Read More

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

Read More

Polyphenol content of cork granulates at different steps of the manufacturing process of microagglomerated stoppers treated with supercritical CO2 used for wine bottling

The wine closure industry is mainly divided into three categories: screw caps, synthetic closures, and cork-based closures. Among this latter, microagglomerated cork stoppers treated with supercritical CO2 are now widely used, especially to avoid cork taint contaminations[1]. They are designed with cork granules obtained from cork offcuts of the punching process during the natural cork stoppers production. A previous study[2] showed that these stoppers released fewer polyphenols in 12 % (v/v) hydroalcoholic solution than natural cork stoppers.

Read More

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

Read More