terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

Abstract

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane. Chitin, the major component of fungal cell walls, is well known to elicit plant immune responses. In Vitis vinifera, knowledge about the perception and immune responses triggered by chitin oligomers is now better understood [1]. Among the LysM receptor-like kinases (LysM-RLKs) family of PRRs, which includes 16 members in Vitis vinifera, three of them (VvLYK1-1, VvLYK1-2 and VvLYK5-1) are involved in the perception of chitin oligomers to trigger plant immune responses [1,2]. Recently, another member of this family, named VvLYK6, was identified in grapevine as being overexpressed following a Botrytis cinerea infection. We characterized the role of VvLYK6 in plant immunity by overexpressing it in Arabidopsis thaliana and grapevine. Surprisingly, overexpression of VvLYK6 decreased chitin-induced MAPK activation, defense gene expression, callose deposition and increased plant sensitivity to fungal infections. According to these results, VvLYK6 negatively regulates chitin-induced defense reactions in grapevine and could be considered as a susceptibility gene in the context of fungal infections.

Acknowledgements: We acknowledge Institut Carnot Plant2Pro for funding the VitiLYKs project and DimaCell platform (Dijon, France) for the confocal microscopy.

References:
1) Roudaire T. et al. (2023) The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYL1-1. Front. Plant Sci. 14:1130782 DOI 10.3389/fpls.2023.1130782

2) Brulé D. et al. (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnol. J. 17, 812–825. DOI 10.1111/pbi.13017

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Villette J.1*, Marzari T.1, Roudaire T.1, Klinguer A.1, Leborgne-Castel N.1, Héloir M-C.1, Poinssot B.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.

Contact the author*

Keywords

grapevine, plant defense, LysM receptor-like kinase, defense inhibition

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution.

Read More

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

Read More

Sensory profile of wines obtained from disease-resistant varieties in La Rioja

The European wine industry is facing multiple challenges derived from climate change and the pressure of different fungal diseases that are compromising the production of traditional varieties. A sustainable alternative maybe the adoption of resistant varieties.
In this study, we have evaluated the enological potential of 9 resistant varieties (5 white and 4 red varieties) in La Rioja. Microvinifications were carried out with three biological replications. Oenological parameters were very diverse with acid content varying from 2.6 g/L to 6.6 g/L.

Read More

Correlative study between degradation of rosé wine under accelerated conditions and under normal conditions

Several studies have tried to develop different methods to study the photodegradation of wine in an accelerated way, trying to elucidate the effect of light on the wine compounds[1]. In a previous study, our team developed a chamber that speeds up the photodegradation of rosé wine[2]. In the present work we have tried to establish a correlation between irradiation times in accelerated conditions and the natural exposure to the cycles of light that usually exist in markets or at home.

Read More

The environmental footprint of selected vineyard management practices: A case study from Logroño (La Rioja) Spain

Viticulture is globally important for socioeconomic and environmental reasons. The EU is globally leading grape and wine production, and Spain is among the top grape and wine producers. As climate change affects viticulture, mitigation and adaptation are crucial for protecting grape production. In this research work, data on viticultural management practices such as soil cultivation, irrigation, energy, machinery, plant protection and the use of fertilizers from vineyards located in Logroño (La Rioja) have been obtained.

Read More