terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

The surprising role of VvLYK6 in grapevine immune responses triggered by chitin oligomers

Abstract

For sustainable viticulture, the substitution of chemical inputs with biocontrol products has become one of the most considered strategies. This strategy is based on elicitor-triggered immunity that requires a deep understanding of the molecular mechanisms involved in plant defense activation. Plant immune responses are triggered through the perception of conserved microbe-associated molecular patterns (MAMPs) which are recognized by pattern recognition receptors (PRRs) at the plasma membrane. Chitin, the major component of fungal cell walls, is well known to elicit plant immune responses. In Vitis vinifera, knowledge about the perception and immune responses triggered by chitin oligomers is now better understood [1]. Among the LysM receptor-like kinases (LysM-RLKs) family of PRRs, which includes 16 members in Vitis vinifera, three of them (VvLYK1-1, VvLYK1-2 and VvLYK5-1) are involved in the perception of chitin oligomers to trigger plant immune responses [1,2]. Recently, another member of this family, named VvLYK6, was identified in grapevine as being overexpressed following a Botrytis cinerea infection. We characterized the role of VvLYK6 in plant immunity by overexpressing it in Arabidopsis thaliana and grapevine. Surprisingly, overexpression of VvLYK6 decreased chitin-induced MAPK activation, defense gene expression, callose deposition and increased plant sensitivity to fungal infections. According to these results, VvLYK6 negatively regulates chitin-induced defense reactions in grapevine and could be considered as a susceptibility gene in the context of fungal infections.

Acknowledgements: We acknowledge Institut Carnot Plant2Pro for funding the VitiLYKs project and DimaCell platform (Dijon, France) for the confocal microscopy.

References:
1) Roudaire T. et al. (2023) The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYL1-1. Front. Plant Sci. 14:1130782 DOI 10.3389/fpls.2023.1130782

2) Brulé D. et al. (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnol. J. 17, 812–825. DOI 10.1111/pbi.13017

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Villette J.1*, Marzari T.1, Roudaire T.1, Klinguer A.1, Leborgne-Castel N.1, Héloir M-C.1, Poinssot B.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.

Contact the author*

Keywords

grapevine, plant defense, LysM receptor-like kinase, defense inhibition

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.

Application of UV-B radiation in pre- and postharvest as an innovative and sustainable cultural practice to improve grape phenolic composition

Ultraviolet radiation (UVR) is a minor part of the solar spectrum, but it represents an important ecological factor that influences many biological processes related to plant growth and development. In recent years, the application of UVR in agriculture and food production is emerging as a clean and environmentally friendly technology.
In grapevine, many studies have been conducted on the effects of ambient levels of UVR, but there are few considering the effects of UV-B application on grape phenolic composition under commercial growing or postharvest conditions.

Atypical aging and hydric stress: insights on an exceptionally dry year

Atypical aging (ATA) is a white wine fault characterized by the appearance of notes of wet rag, acacia blossoms and naphthalene, along with the vanishing of varietal aromas. 2-aminoacetophenone (AAP) – a degradation compound of indole-3-acetic acid (IAA) – is regarded as the main sensorial and chemical marker responsible for this defect. About the origin of ATA, a stress reaction occurring in the vineyard has been looked as the leading cause of this defect. Agronomic, climatic and pedological factors are the main triggers and among them, drought stress seems to play a crucial role.[1]