terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Abstract

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential. Thus, there is a scope for innovation in grapevine rootstock varieties by exploiting a wider range of wild Vitis species. The aim of this study was to investigate the drought adaptation potential of a wide range of previously unexplored wild Vitis species, integrating information at different biological scales: from genomics, transcriptomics and metabolomics to developmental and functional root traits. Our hypothesis is that metabolites can be considered as intermediate phenotypes in roots because they are expected to be mechanistically related to more complex root phenotypes. For these purposes, we investigated genetic differentiation between species and variability within species using SNPs obtained from Illumina paired-end whole genome sequencing (4k project)[1]. In addition, we characterized the response of cuttings from 12 American, Asian and Eurasian wild Vitis species (50 accessions in total) to moderate drought, during 1 month in a greenhouse experiment, with a focus on root morphological, functional and molecular responses (transcriptomics and metabolomics). We identified variability among species in root osmotic adjustment, an important mechanism of drought tolerance[2]. The link between root osmotic potential and metabolites differentially accumulated in response to drought was analyzed. The results obtained in this work will help to identify new genetic resources to be used in rootstocks breeding programs to improve drought adaptation. In addition, we will provide tools to accelerate the screening of desirable traits in rootstocks using intermediate molecular phenotypes as proxies of complex root responses.

Acknowledgements:

We received funding from INRAE, University of Bordeaux (project PurVitis), Nouvelle-Aquitaine region (project VitiScope) and CNIV. We acknowledge Maria Lafargue, Cyril Hevin, Nicolas Hocquard and Jean-Pierre Petit for their help with the plant material preparation.

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892‑901.
2)  Blum, A. (2017). Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant, Cell & Environment, 40(1), 4‑10.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Marina de Miguel1*, Etienne R. Patin1, Elsa Chedid1, Enrique Saez-Laguna2, Ander del Sol Iturralde3, Usue Pérez-Lopez3, Jean-Pascal Tandonnet1, Pierre-François Bert1, Philippe Vivin1, Elisa Marguerit1, Sarah Cookson1, Nathalie Ollat1

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France
2 INRAE, Univ. Bordeaux, BIOGECO, Cestas, France
3 Departamento de Biología Vegetal y Ecología, Facultad de Ciencia y Tecnología, Universidad del País Vasco, UPV/EHU, Apdo. 644, 48080, Bilbao, Spain

Contact the author*

Keywords

genetic variability, drought, metabolomics, osmotic adjustment, roots

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effects of laccase from Botrytis cinerea on the oxidative degradation kinetics of the five natural grape anthocyanins

Enzymatic browning[1] is an oxidation process that occurs in many foods that increases the brown colour[2]. This problem is especially harmful in the wine industry[3]. especially when the grapes are infected by grey rot since this fung release the oxidative enzyme laccase[4]. In the particular case of red wines, the presence of laccase implies the deterioration of the red colour and can even cause the precipitation of the coloring matter (oxidasic haze)[5].

Limiting magnesium availability: a novel approach to managing brettanomyces spoilage in winemaking

Brettanomyces is a world-renowned yeast that negatively impacts the chemical composition of wines through the production of metabolites that negatively impact the sensory properties of the final product. Its resilience in wine conditions and ability to produce off-flavors make it a challenge for winemakers. Currently, the primary control technique involves adding sulfur dioxide (SO2); however, some Brettanomyces strains are developing resistance to this preservative agent. [1] Therefore, new management strategies are necessary to control this spoilage yeast.

New tool to evaluate color modifications during oxygen consumption in white and red wines

Measuring the effect of oxygen consumption on the color of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine can consume without significantly altering its color. The changes produced in wine after being exposed to high oxygen concentrations have been studied by different authors, but in all cases the wine has been analyzed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen[1,2].

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison.