terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Abstract

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential. Thus, there is a scope for innovation in grapevine rootstock varieties by exploiting a wider range of wild Vitis species. The aim of this study was to investigate the drought adaptation potential of a wide range of previously unexplored wild Vitis species, integrating information at different biological scales: from genomics, transcriptomics and metabolomics to developmental and functional root traits. Our hypothesis is that metabolites can be considered as intermediate phenotypes in roots because they are expected to be mechanistically related to more complex root phenotypes. For these purposes, we investigated genetic differentiation between species and variability within species using SNPs obtained from Illumina paired-end whole genome sequencing (4k project)[1]. In addition, we characterized the response of cuttings from 12 American, Asian and Eurasian wild Vitis species (50 accessions in total) to moderate drought, during 1 month in a greenhouse experiment, with a focus on root morphological, functional and molecular responses (transcriptomics and metabolomics). We identified variability among species in root osmotic adjustment, an important mechanism of drought tolerance[2]. The link between root osmotic potential and metabolites differentially accumulated in response to drought was analyzed. The results obtained in this work will help to identify new genetic resources to be used in rootstocks breeding programs to improve drought adaptation. In addition, we will provide tools to accelerate the screening of desirable traits in rootstocks using intermediate molecular phenotypes as proxies of complex root responses.

Acknowledgements:

We received funding from INRAE, University of Bordeaux (project PurVitis), Nouvelle-Aquitaine region (project VitiScope) and CNIV. We acknowledge Maria Lafargue, Cyril Hevin, Nicolas Hocquard and Jean-Pierre Petit for their help with the plant material preparation.

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892‑901.
2)  Blum, A. (2017). Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant, Cell & Environment, 40(1), 4‑10.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Marina de Miguel1*, Etienne R. Patin1, Elsa Chedid1, Enrique Saez-Laguna2, Ander del Sol Iturralde3, Usue Pérez-Lopez3, Jean-Pascal Tandonnet1, Pierre-François Bert1, Philippe Vivin1, Elisa Marguerit1, Sarah Cookson1, Nathalie Ollat1

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France
2 INRAE, Univ. Bordeaux, BIOGECO, Cestas, France
3 Departamento de Biología Vegetal y Ecología, Facultad de Ciencia y Tecnología, Universidad del País Vasco, UPV/EHU, Apdo. 644, 48080, Bilbao, Spain

Contact the author*

Keywords

genetic variability, drought, metabolomics, osmotic adjustment, roots

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The environmental footprint of selected vineyard management practices: A case study from Logroño (La Rioja) Spain

Viticulture is globally important for socioeconomic and environmental reasons. The EU is globally leading grape and wine production, and Spain is among the top grape and wine producers. As climate change affects viticulture, mitigation and adaptation are crucial for protecting grape production. In this research work, data on viticultural management practices such as soil cultivation, irrigation, energy, machinery, plant protection and the use of fertilizers from vineyards located in Logroño (La Rioja) have been obtained.

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

Evaluation of terroir suitability for vine cultivation in new areas using geographic multi-criteria decision support

Based on historical vine cultivation, the recent development of wine production in Drama wine region (Greece) has led to vine cultivation expansion of white and red varieties. The current cultivation of 500 ha of vineyards is expected to increase in the coming years. Natural terroir units (NTU) have been designed recently to support the production of high quality wines in the region [1]. The aim of this work is to evaluate the relevancy of the proposed NTUs regarding their suitability to produce wines of specific sensorial identity, and to provide guidelines for correct site selection for the expanding wine industry of the region.

Study of Spanish wine sensory analysis data over a 3-year period

This study presents an investigation based on sensory analysis data of Spanish wines with geographical indications collected over a three-year period. Sensory analysis plays a crucial role in assessing the quality, characteristics, and perception of wines. The trained tasting panel at Dolmar Laboratory, accredited for objective sensory evaluation of wines since 2016, has been tasting over 5000 wines. However, it is since 2021, when a computer application for tastings was developed, that the digitalization of data allows for detailed statistical analysis of the results.

How are canned wine drinkers perceived? An investigation involving Swiss nationals and different scenarios of outdoor leisure activities

This study examines how people who consume wine in cans are perceived in terms of their basic personality characteristics, helps understand the role of cultural background on people’s perception, and verify the role played by the consumption context on the perception. Our hypothesis is that prejudice and negative attitudes towards wine in cans might exert a negative effect on the evaluation of people who consume canned wine. To evaluate this hypothesis, the consumption of wine in cans was evoked in four different contexts of use during outdoor leisure activity (beach resort, ski resort, desert safari, and party). In order to examine the effect of culture on subject’s response we use participants from Switzerland, a country where three different cultures, associated with three different languages, cohabit.