terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Abstract

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential. Thus, there is a scope for innovation in grapevine rootstock varieties by exploiting a wider range of wild Vitis species. The aim of this study was to investigate the drought adaptation potential of a wide range of previously unexplored wild Vitis species, integrating information at different biological scales: from genomics, transcriptomics and metabolomics to developmental and functional root traits. Our hypothesis is that metabolites can be considered as intermediate phenotypes in roots because they are expected to be mechanistically related to more complex root phenotypes. For these purposes, we investigated genetic differentiation between species and variability within species using SNPs obtained from Illumina paired-end whole genome sequencing (4k project)[1]. In addition, we characterized the response of cuttings from 12 American, Asian and Eurasian wild Vitis species (50 accessions in total) to moderate drought, during 1 month in a greenhouse experiment, with a focus on root morphological, functional and molecular responses (transcriptomics and metabolomics). We identified variability among species in root osmotic adjustment, an important mechanism of drought tolerance[2]. The link between root osmotic potential and metabolites differentially accumulated in response to drought was analyzed. The results obtained in this work will help to identify new genetic resources to be used in rootstocks breeding programs to improve drought adaptation. In addition, we will provide tools to accelerate the screening of desirable traits in rootstocks using intermediate molecular phenotypes as proxies of complex root responses.

Acknowledgements:

We received funding from INRAE, University of Bordeaux (project PurVitis), Nouvelle-Aquitaine region (project VitiScope) and CNIV. We acknowledge Maria Lafargue, Cyril Hevin, Nicolas Hocquard and Jean-Pierre Petit for their help with the plant material preparation.

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892‑901.
2)  Blum, A. (2017). Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant, Cell & Environment, 40(1), 4‑10.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Marina de Miguel1*, Etienne R. Patin1, Elsa Chedid1, Enrique Saez-Laguna2, Ander del Sol Iturralde3, Usue Pérez-Lopez3, Jean-Pascal Tandonnet1, Pierre-François Bert1, Philippe Vivin1, Elisa Marguerit1, Sarah Cookson1, Nathalie Ollat1

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France
2 INRAE, Univ. Bordeaux, BIOGECO, Cestas, France
3 Departamento de Biología Vegetal y Ecología, Facultad de Ciencia y Tecnología, Universidad del País Vasco, UPV/EHU, Apdo. 644, 48080, Bilbao, Spain

Contact the author*

Keywords

genetic variability, drought, metabolomics, osmotic adjustment, roots

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Unveiling a hidden link: does time hold the key to altered spectral signatures of grapevines under drought?

Remote sensing technology captures spectral data beyond the visible range, making it useful for monitoring plant stress. Vis-NIR (Visible-Near Infrared) spectroscopy (400-1000 nm) is commonly used to indirectly assess plant status during drought. One example is the widespread use of normalized difference vegetation index (NDVI) that is strongly linked to green biomass. However, a knowledge gap exists regarding the applicability of this method to all the drought conditions and if it is a direct correlation to the water status of the plant.

Evaluation of phenology, agronomic and oenological quality in minority wine varieties in Madrid as a strategy for adaptation to climate change

The main phenological stages (budburst, flowering, veraison, and ripeness) and the fruit composition of 34 Spanish minority varieties were studied to determine their cultivation potential and help winegrowers adapt their production systems to climate change conditions. In total, 4 control cultivars, and 30 minority varieties from central Spain were studied during a period of 3 campaigns, in the ampelographic collection “El Encín”, in Alcalá de Henares, Madrid. Agronomic and oenological characteristics such as yield, and total soluble solids concentration have been monitored.

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

What to do to solve the riddle of vine rootstock induced drought tolerance

Climate change will increase the frequency of water deficit situation in some European regions, by the increase of the evapotranspiration and the reduction of rainfalls during the growing cycle. This requires finding ways of adaptation, including the use of plant material which is more tolerant to drought. In addition to the varieties used as scions that result in the typicality of wines, rootstocks constitute a relevant way of adaptation to more stressful environmental conditions.

The environmental footprint of selected vineyard management practices: A case study from Logroño (La Rioja) Spain

Viticulture is globally important for socioeconomic and environmental reasons. The EU is globally leading grape and wine production, and Spain is among the top grape and wine producers. As climate change affects viticulture, mitigation and adaptation are crucial for protecting grape production. In this research work, data on viticultural management practices such as soil cultivation, irrigation, energy, machinery, plant protection and the use of fertilizers from vineyards located in Logroño (La Rioja) have been obtained.