terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Abstract

Global warming and increased frequency and/or severity of drought events are among the most threatening consequences of climate change for agricultural crops. In response to drought, grapevine (as many other plants) exhibits osmotic adjustment through active accumulation of osmolytes which in turn shift the leaf turgor loss point (TLP) to more negative values, allowing to maintain stomata opened at lower water potentials1. We investigated the capacity of Pinot noir leaves to modulate their osmotic potential as a function of: (i) time (seasonal osmoregulation), (ii) growing temperatures, and (iii) drought events, to enhance comprehension of the resilience of grapevines in drought conditions. We performed trails under semi-controlled field conditions, and in two different greenhouse chambers (20/15 °C vs 25/20 °C day/night). For two consecutive vegetative seasons, grafted potted grapevines (Pinot noir/SO4) were subjected to two different water regimes for at least 30 days: well-watered (WW) and water deficit (WD). Via pressure-volume (PV) curves and osmometer measurements we derived the leaf osmotic adjustment capability and TLP, while monitoring the plant gas exchange and water potential. Surprisingly, lower water potentials in WD vines throughout the season and in all situations (field and greenhouse) did not trigger osmoregulation, changes in TLP nor a modification of the modulus of elasticity. PV curves provided clear evidence that both temperature and water availability do not stimulate active osmotic adjustment in Vitis vinifera cv. Pinot Noir. Conversely, there is a clear impact of seasonal osmoregulation throughout the growing season2, decreasing the osmotic potential at full turgor by an average of 0.46 MPa in 90 days. Lack of osmotic adjustment in response to drought observed in this cultivar suggests Vitis genotypes have a broad spectrum of responses to drought and the strategy adopted to cope with it is highly dependent on the cultivar under analysis.

Acknowledgements:

The work was financially supported by the Austrian Science Fund (FWF): I 4848 “PlasticGrape”.

References:

1)  Bartlett, M. K., Scoffoni, C., & Sack, L. (2012). The determinants of leaf turgor loss point and prediction of drought tolerance of species and biomes: a global metaanalysis. Ecology letters15(5), 393-405.

2)  Herrera, J. C., Calderan, A., Gambetta, G. A., Peterlunger, E., Forneck, A., Sivilotti, P., … & Hochberg, U. (2022). Stomatal responses in grapevine become increasingly more tolerant to low water potentials throughout the growing season. The Plant Journal109(4), 804-815.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Elena Farolfi1*, Francesco Flagiello2, Federica De Berardinis1, Soma Laszlo Tarnay1, Jan Reščič3, Astrid Forneck1, Jose Carlos Herrera1

1University of Natural Resources and Life Sciences, Vienna, Department of Crop Sciences, Institute of Viticulture and Pomology, 3430 Tulln an der Donau, Austria
2University of Natural Resources and Life Sciences, Vienna, Department of Crop Sciences, Institute of agronomy, 3430 Tulln an der Donau, Austria
3University of Nova Gorica, School for Viticulture and Enology, Dvorec Lanthieri/Lanthieri Mansion Glavni trg 8, 5271 Vipava, Slovenia

Contact the author*

Keywords

grapevine, drought, osmotic adjustment, osmoregulation, turgor loss point

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Reconstructing ancient microbial fermentation genomes from the wine residues of Herod, Roman king of Judea

The fortress of the Herodium, built towards the end of the first century BCE/ante Cristo, on the orders of Herod the Great, Roman client king of Judea, attests the expansion of Roman influence in the eastern Mediterranean. During archaeological excavations of the Herodium in 2017[1], a winery was discovered on the ground floor of the palace, with an assortment of clay vessels in situ, including large dolia – clay fermentation vessels each capable of fermenting up to 300-400 L of wine. Thanks to the recent progresses in the field of paleogenomics[2], we could analyse the organic material consistent with grape pomace at the bottom of these vessels, by extracting and sequencing the DNA using shotgun metagenomics and targeted capture, aiming for enrichment of DNA from fermentation associated microbes.

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character.

High-throughput screening of physical-mechanical berry skin traits facilitates targeted selection of breeding material with resistance to Botrytis bunch rot and grape sunburn

The ongoing climate change implies an increasing mean air temperature, which is signified by weather extremes or sudden changes between drought and local heavy rainfalls. These changing conditions are especially challenging for the established grapevine varieties growing under cool climate conditions due to an increased risk for fungal diseases like downy mildew (DM) and Botrytis bunch rot (BBR) as well as for grape sunburn. To meet that demand, the scope of most grapevine breeding programs is the selection of mildew fungus-resistant and climatic adapted grapevines with balanced, healthy yield and outstanding wine quality.

Study of Spanish wine sensory analysis data over a 3-year period

This study presents an investigation based on sensory analysis data of Spanish wines with geographical indications collected over a three-year period. Sensory analysis plays a crucial role in assessing the quality, characteristics, and perception of wines. The trained tasting panel at Dolmar Laboratory, accredited for objective sensory evaluation of wines since 2016, has been tasting over 5000 wines. However, it is since 2021, when a computer application for tastings was developed, that the digitalization of data allows for detailed statistical analysis of the results.

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.