terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Abstract

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Therefore, we tested the effect of Ta SC1and Bs PTA-271, on grapevine rhizosphere bacterial and fungal microbiome, and plant gene expression, in two different soil types (clay and sandy soil). Additionally, we specifically quantified Ta SC1 and Bs PTA-271 in rhizosphere of both soil types. To do that, we used novel molecular approaches, such as high-throughput amplicon sequencing (HTAS), droplet digital PCR (ddPCR) and real-time PCR (qPCR). The results suggest that Bs PTA-271 established better in clay soil, where although its quantity was low at 30dpi, it was maintained at 90dpi. In contrast, Ta SC1 established better in sandy soil, with a lower quantity, that increased overtime. Regarding the BCAs impact on rhizosphere microbiome, bacterial diversity was not affected by any BCA application. However, inoculations with Ta SC1 significantly reduced fungal diversity. Ta SC1 applications affected the relationships between microorganisms, however this effect was clearer in clay soil. According to the selected plant defense markers, plants living in sandy soil appeared more impacted to BCA inoculation. Ta SC1 plants treated in sandy soil demonstrated a strong suppression defense genes 24hpi, that conversed in strong simulated defenses 4dpi, with a significant overexpression of PAL and STS. On the other hand, in clay soil, BCA-treated plants showed a slight increase in the expression of plant defense genes 24hpi, that intensifies 4dpi. In conclusion, the effect of Bs PTA-271 and Ta SC1 in grapevine rhizosphere appears to be soil-dependent, where a sandy soil favors the fungal BCA (Ta SC1) establishment, while a clay soil favors the bacterial BCA (Bs PTA-271) establishment.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Catarina Leal1, Rebeca Bujanda1, Josep Armengol2, Patricia Trotel-Aziz3, Florence Fontaine3, Ales Eichmeier4, David Gramaje1

1Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain
2Instituto Agroflorestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain
3University of Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes Research Unit, EA 4707, INRAE USC 1488, SFR Condorcet FR CNRS 3417, Reims, France
4Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic

Contact the author*

Keywords

Bacillus spp., biocontrol, ddPCR, grapevine, microbiome, next generation sequencing, rhizosphere, Trichoderma spp.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The combined use of Lachancea thermotolerans and lactic bacteria in wine technology

The production of most red wines that are sold involves an alcoholic fermentation carried out by yeasts of the Saccharomyces genus, and a subsequent fermentation carried out by lactic bacteria of the Oenococus oeni species after the first one is fully completed. However, the traditional process can face complications, which can be more likely in grape juices with high levels of sugar and pH. Because of climate change, these situations are more frequent in the wine industry. The main hazards in those scenarios are halts or delays in the alcoholic fermentation or the growth of unwanted bacteria while the alcoholic fermentation is not done yet and the wine still has residual sugars.

Dynamics of Saccharomyces cerevisiae population in spontaneous fermentations from Granxa D’Outeiro terroir (DOP Ribeiro, NW Spain)

Granxa D’Outeiro is a recovered ancient vineyard located in the heart of DOP Ribeiro, where traditional white grapevine varieties are growing under sustainable management. Spontaneous fermentations using grape must from Treixadura, Albariño, Lado, Godello, and Loureira varieties were carried out at experimental winery of Evega. Yeasts were isolated from must and at different stages of fermentation. Those colonies belonging to Saccharomyces cerevisiae were characterized at strain level by mDNA-RFLPs.

Reduction of the height of the canopy in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

Global warming is accelerating the technological ripening of the grape, with a loss of acidity, which requires that vineyard management can delay ripening to avoid it. The source-sink relation is essential for grape ripening, since it affects the distribution of photosynthates and substances derived from plant metabolism. A work is proposed to know the response of the vineyard to the drastic reduction of the foliar surface by trim down the shoots in cv.

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.