terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Abstract

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Therefore, we tested the effect of Ta SC1and Bs PTA-271, on grapevine rhizosphere bacterial and fungal microbiome, and plant gene expression, in two different soil types (clay and sandy soil). Additionally, we specifically quantified Ta SC1 and Bs PTA-271 in rhizosphere of both soil types. To do that, we used novel molecular approaches, such as high-throughput amplicon sequencing (HTAS), droplet digital PCR (ddPCR) and real-time PCR (qPCR). The results suggest that Bs PTA-271 established better in clay soil, where although its quantity was low at 30dpi, it was maintained at 90dpi. In contrast, Ta SC1 established better in sandy soil, with a lower quantity, that increased overtime. Regarding the BCAs impact on rhizosphere microbiome, bacterial diversity was not affected by any BCA application. However, inoculations with Ta SC1 significantly reduced fungal diversity. Ta SC1 applications affected the relationships between microorganisms, however this effect was clearer in clay soil. According to the selected plant defense markers, plants living in sandy soil appeared more impacted to BCA inoculation. Ta SC1 plants treated in sandy soil demonstrated a strong suppression defense genes 24hpi, that conversed in strong simulated defenses 4dpi, with a significant overexpression of PAL and STS. On the other hand, in clay soil, BCA-treated plants showed a slight increase in the expression of plant defense genes 24hpi, that intensifies 4dpi. In conclusion, the effect of Bs PTA-271 and Ta SC1 in grapevine rhizosphere appears to be soil-dependent, where a sandy soil favors the fungal BCA (Ta SC1) establishment, while a clay soil favors the bacterial BCA (Bs PTA-271) establishment.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Catarina Leal1, Rebeca Bujanda1, Josep Armengol2, Patricia Trotel-Aziz3, Florence Fontaine3, Ales Eichmeier4, David Gramaje1

1Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain
2Instituto Agroflorestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain
3University of Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes Research Unit, EA 4707, INRAE USC 1488, SFR Condorcet FR CNRS 3417, Reims, France
4Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic

Contact the author*

Keywords

Bacillus spp., biocontrol, ddPCR, grapevine, microbiome, next generation sequencing, rhizosphere, Trichoderma spp.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Oxidability of wines made from Spanish minority grape varieties

The phenolic profile of a wine plays an essential role in its oxidative capacity and in both white and red wines it defines its shelf life[1]. The study of minority varieties to produce wines with peculiar characteristics necessarily includes the phenolic and oxidative characterization of the wines produced. This paper presents the study of wines made from 24 minority and majority white and red grape varieties, focusing on phenolic characteristics (total phenols, slightly polymerized phenols, highly polymerized phenols, anthocyanins…), color, as well as parameters related to the oxidability of the wines and their capacity to consume oxygen [2].

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential.

Selecting green cover species in the under-trellis zone of Lower Austrian vineyards

The under-trellis zone of vineyards is a sensitive area through which vines cover a significant portion of their nutrient and water needs. Mechanical and chemical methods are applied to suppress competing and tall-growing weeds to ensure optimal vine growth conditions. In addition to higher operating costs and depending on the soil conditions, these practices might lead to a long-term reduction in soil fertility and biodiversity. The presented study aims to analyse the suitability and interspecies competition of a selected green cover mixture of five local herbaceous species as potential green cover mixture in the under-trellis area of Lower Austrian vineyards.

Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

The use of grapevine genetic diversity is a way to mitigate the negative impacts of climate change on viticulture systems. Leaf epidermal flavonoids (including flavonols and anthocyanins) are involved in plant defense mechanisms against environmental stresses, like high temperatures or excessive solar radiation [1,2]. Among other factors, they modulate light absorption, which reduces photoinhibition processes in photosynthetic tissues [1]. Therefore, the identification of grapevine cultivars with an increased content on leaf epidermal flavonoids arises as a potential avenue to improve grapevine tolerance to some detrimental environmental stresses.

Phenotyping bud break and trafficking of dormant buds from grafted vine

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1].