terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Abstract

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Therefore, we tested the effect of Ta SC1and Bs PTA-271, on grapevine rhizosphere bacterial and fungal microbiome, and plant gene expression, in two different soil types (clay and sandy soil). Additionally, we specifically quantified Ta SC1 and Bs PTA-271 in rhizosphere of both soil types. To do that, we used novel molecular approaches, such as high-throughput amplicon sequencing (HTAS), droplet digital PCR (ddPCR) and real-time PCR (qPCR). The results suggest that Bs PTA-271 established better in clay soil, where although its quantity was low at 30dpi, it was maintained at 90dpi. In contrast, Ta SC1 established better in sandy soil, with a lower quantity, that increased overtime. Regarding the BCAs impact on rhizosphere microbiome, bacterial diversity was not affected by any BCA application. However, inoculations with Ta SC1 significantly reduced fungal diversity. Ta SC1 applications affected the relationships between microorganisms, however this effect was clearer in clay soil. According to the selected plant defense markers, plants living in sandy soil appeared more impacted to BCA inoculation. Ta SC1 plants treated in sandy soil demonstrated a strong suppression defense genes 24hpi, that conversed in strong simulated defenses 4dpi, with a significant overexpression of PAL and STS. On the other hand, in clay soil, BCA-treated plants showed a slight increase in the expression of plant defense genes 24hpi, that intensifies 4dpi. In conclusion, the effect of Bs PTA-271 and Ta SC1 in grapevine rhizosphere appears to be soil-dependent, where a sandy soil favors the fungal BCA (Ta SC1) establishment, while a clay soil favors the bacterial BCA (Bs PTA-271) establishment.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Catarina Leal1, Rebeca Bujanda1, Josep Armengol2, Patricia Trotel-Aziz3, Florence Fontaine3, Ales Eichmeier4, David Gramaje1

1Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain
2Instituto Agroflorestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain
3University of Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes Research Unit, EA 4707, INRAE USC 1488, SFR Condorcet FR CNRS 3417, Reims, France
4Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic

Contact the author*

Keywords

Bacillus spp., biocontrol, ddPCR, grapevine, microbiome, next generation sequencing, rhizosphere, Trichoderma spp.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Entomopathogenic nematodes application for controlling Lobesia botrana in grapevine and their impact on grapevine quality 

Entomopathogenic nematodes (EPN) are well-known biological control agents combined with specific adjuvants that now allow their use against aerial pests. Lobesia botrana (Lepidoptera: Tortricidae) is one of the major harmful pests detected in worldwide vineyards. Previous studies demonstrated that the EPNs Steinernema feltiae and S. carpocapsae could control L. botrana. The hypothesis was that the best combination of EPN-adjuvant/timing (season/temperatures) will support the use of EPN in the vineyard against L. botrana with no impact on the grape performance.

Grapevine adaptation to drought and resistance to Neofusicoccum parvum, causal agent of Botryosphaeria dieback

The sustainability of viticulture in response to climate change has been addressed mainly considering agronomic impacts, such as water management and diseases, either separately or together.
In grapevines, there is strong evidence that different genotypes respond differently to biotic and abiotic stresses. A screening was conducted on various local cultivars in response to drought and Neofusicoum parvum infection aiming to evaluate their susceptibility to abiotic stress and resistance to fungal diseases.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Combined abiotic-biotic plant stresses on the roots of grapevine

In the 19th century, devastating outbreaks of phylloxera (Daktulosphaira vitifoliae Fitch), almost brought European viticulture to its knees. Phylloxera does not only take energy in form of sugars from the vine, but also affects the up- and down- regulations of genes, acts as a carbon sink and reprograms the physiology of the grapevines, including nutrient uptake and the defense system [1]. A key trait of rootstocks is the ability to perform well under high lime conditions as about 30 % of the land surface has calcareous soil. Iron deficiency not only causes the well-known problems of lime-induced chlorosis and stunted growth, but also affects the entire plant metabolism.

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.