terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Abstract

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Therefore, we tested the effect of Ta SC1and Bs PTA-271, on grapevine rhizosphere bacterial and fungal microbiome, and plant gene expression, in two different soil types (clay and sandy soil). Additionally, we specifically quantified Ta SC1 and Bs PTA-271 in rhizosphere of both soil types. To do that, we used novel molecular approaches, such as high-throughput amplicon sequencing (HTAS), droplet digital PCR (ddPCR) and real-time PCR (qPCR). The results suggest that Bs PTA-271 established better in clay soil, where although its quantity was low at 30dpi, it was maintained at 90dpi. In contrast, Ta SC1 established better in sandy soil, with a lower quantity, that increased overtime. Regarding the BCAs impact on rhizosphere microbiome, bacterial diversity was not affected by any BCA application. However, inoculations with Ta SC1 significantly reduced fungal diversity. Ta SC1 applications affected the relationships between microorganisms, however this effect was clearer in clay soil. According to the selected plant defense markers, plants living in sandy soil appeared more impacted to BCA inoculation. Ta SC1 plants treated in sandy soil demonstrated a strong suppression defense genes 24hpi, that conversed in strong simulated defenses 4dpi, with a significant overexpression of PAL and STS. On the other hand, in clay soil, BCA-treated plants showed a slight increase in the expression of plant defense genes 24hpi, that intensifies 4dpi. In conclusion, the effect of Bs PTA-271 and Ta SC1 in grapevine rhizosphere appears to be soil-dependent, where a sandy soil favors the fungal BCA (Ta SC1) establishment, while a clay soil favors the bacterial BCA (Bs PTA-271) establishment.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Catarina Leal1, Rebeca Bujanda1, Josep Armengol2, Patricia Trotel-Aziz3, Florence Fontaine3, Ales Eichmeier4, David Gramaje1

1Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain
2Instituto Agroflorestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain
3University of Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes Research Unit, EA 4707, INRAE USC 1488, SFR Condorcet FR CNRS 3417, Reims, France
4Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic

Contact the author*

Keywords

Bacillus spp., biocontrol, ddPCR, grapevine, microbiome, next generation sequencing, rhizosphere, Trichoderma spp.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

The foliar application of urea has been shown to be able to satisfy the specific nutritional needs of the vine as well as to increase the nitrogen composition of the must. On the other hand, the use of nanotechnology could be of great interest in viticulture as it would help to slow down the release of urea and protect it against possible degradation. Several studies indicate that cell wall synthesis and remodeling are affected by nitrogen availability.

Effects of different soil types and soil management on greenhouse gas emissions 

Soil is important in the carbon cycle and the dynamics of greenhouse gases (CO2, CH4 and N2O). Key soil characteristics, such as organic matter content, texture, structure, pH and microbial activity, play a determining role in GHG emissions[1]. The objective of the study is to delimit different types of soil, with different soil management and to be able to verify the differences in CO2, CH4 and N2O emissions. The study was carried out in a vineyard of Bodegas Campo Viejo in Logroño (La Rioja), whose plant material is Vitis vinifera L. cv. Tempranillo.

Effect of different plant fibers on the elimination of undesirable compounds in red wine. Correlation with its polysaccharide composition

The presence of undesirable compounds in wines, such as OTA, biogenic amines and pesticides residues, affects wine quality and can cause health problems for the consumer. The main tool that a winemaker has to reduce their content in the wine is fining. However, some of the fining agents commonly used in the winery can cause allergies or even increase the protein content in the wine, increasing the turbidity. To avoid these problems, the use of plant fibers may be an alternative, such as those from grape pomace[1] or other plant origins.

Perception, liking and emotional response of tropical fruit aromas in Chardonnay wines

Tropical fruit aromas in wines are thought to be important to wine consumers, although there is little research to confirm this statement. With so many wine styles available, it has become important to understand the qualities that are desirable to consumers and how to achieve those qualities. Thiols and esters are compounds that have been found to cause tropical fruit aromas in chardonnay (ref). Fermentation temperature gradients and skin contact were found to increase these compounds using micro scale fermentations. This work aimed to scale up these fermentations/operations to determine if the desired tropical fruit aromas could still be achieved and if there is a perceivable difference in tropical fruit aromas, liking, and emotional response in the wines at the consumer level.

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.