terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Abstract

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses. Therefore, we tested the effect of Ta SC1and Bs PTA-271, on grapevine rhizosphere bacterial and fungal microbiome, and plant gene expression, in two different soil types (clay and sandy soil). Additionally, we specifically quantified Ta SC1 and Bs PTA-271 in rhizosphere of both soil types. To do that, we used novel molecular approaches, such as high-throughput amplicon sequencing (HTAS), droplet digital PCR (ddPCR) and real-time PCR (qPCR). The results suggest that Bs PTA-271 established better in clay soil, where although its quantity was low at 30dpi, it was maintained at 90dpi. In contrast, Ta SC1 established better in sandy soil, with a lower quantity, that increased overtime. Regarding the BCAs impact on rhizosphere microbiome, bacterial diversity was not affected by any BCA application. However, inoculations with Ta SC1 significantly reduced fungal diversity. Ta SC1 applications affected the relationships between microorganisms, however this effect was clearer in clay soil. According to the selected plant defense markers, plants living in sandy soil appeared more impacted to BCA inoculation. Ta SC1 plants treated in sandy soil demonstrated a strong suppression defense genes 24hpi, that conversed in strong simulated defenses 4dpi, with a significant overexpression of PAL and STS. On the other hand, in clay soil, BCA-treated plants showed a slight increase in the expression of plant defense genes 24hpi, that intensifies 4dpi. In conclusion, the effect of Bs PTA-271 and Ta SC1 in grapevine rhizosphere appears to be soil-dependent, where a sandy soil favors the fungal BCA (Ta SC1) establishment, while a clay soil favors the bacterial BCA (Bs PTA-271) establishment.

DOI:

Publication date: October 4, 2023

Issue: ICGWS 2023

Type: Article

Authors

Catarina Leal1, Rebeca Bujanda1, Josep Armengol2, Patricia Trotel-Aziz3, Florence Fontaine3, Ales Eichmeier4, David Gramaje1

1Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain
2Instituto Agroflorestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain
3University of Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes Research Unit, EA 4707, INRAE USC 1488, SFR Condorcet FR CNRS 3417, Reims, France
4Mendeleum—Institute of Genetics, Mendel University in Brno, Valticka 334, 691 44 Lednice, Czech Republic

Contact the author*

Keywords

Bacillus spp., biocontrol, ddPCR, grapevine, microbiome, next generation sequencing, rhizosphere, Trichoderma spp.

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements. In each condition, the same two wines were blind tasted: a wine previously defined as delicate – a Pinot Noir from Australia, and a wine known for its robust character – a Tannat from Uruguay.

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities.

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

Organic mulches slightly influence wine phenolic composition and sensorial properties

Grapevines have traditionally been grown in semi-arid areas, but viticulture is now compromised by climate change. Therefore, it is necessary to implement environmentally friendly viticulture practices to adapt grapevines to current climatic conditions. In this context, organic mulches offer many benefits, such as reduced soil erosion and increased organic matter, soil water content and crop productivity. However, these practices must not compromise grape and wine quality. Therefore, the objective of this study was to evaluate the effect on wine physicochemical and phenolic composition and sensorial properties of different soil management practices on the vine row. Over four years, five soil treatments were examined in two different vineyards.

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum).