OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Chemical and Biochemical reactions, including grape and wines microorganisms impact 9 What is the fate of oxygen consumed by red wine? Main processes and reaction products

What is the fate of oxygen consumed by red wine? Main processes and reaction products

Abstract

Oxygen consumed by wine is used to oxidize sulfur dioxide and ethanol to form acetaldehyde wine oxygen consumption rate (OCR) was negatively correlated with the initial acetaldehyde level. Experiences carried out at 25 ºC with red wines have demonstrated that after consuming a large amount of O2, some young wines did not form acetaldehyde. However, acetaldehyde level increased in aged wines. Higher acetaldehyde accumulation in aged wines can be explained by Aldehyde Reactive Polyphenols (ARPs) smaller amounts, because of their lower reactive potential due to high O2 exposure. Models characterized ARPs as anthocyanins, flavonols, tannins and flavanol-anthocyanins adducts. These ARPs should be closely related to wine aging potential by measuring acetaldehyde consumption rate (ACRs) and/or the maxima amounts of acetaldehyde each wine can consume. 

The main goal of this work was to find a new polyphenol index which should be linked to wine oxygen consumption kinetics. It could indicate the maximum oxygen level that a wine can consume. As well as, elucidate if acetaldehyde is the reactive species with ARPs, but one of its radical precursors in the Fenton reaction. 

Three experiments were prepared in anoxia followed by total acetaldehyde determination by using HPLC: 1) wines spiked with 30 and 300 mg/L of acetaldehyde and incubated at 25, 45 and 70 °C; 2)synthetic wines spiked with 15 to 120 mg/L of acetaldehyde and polyphenol extracts; 3) synthetic matrices filled with malvidin-3-O-glucoside, catechin and a mix of both, which were exposed to: a) 8 mg/L O2 to form acetaldehyde in situ or b) to anoxia and spiked acetaldehyde (11 mg/L). 

Several wines consume acetaldehyde at different rates, which are particularly imprecise at low temperatures. This makes impractical the use of ACRs as an index to categorize wine polyphenolic composition by defining a discrete ARP category. ACRs are too complex, showing a high dependence order towards acetaldehyde level and an equilibrium concentration. Such concentrations were found to depend on the previous acetaldehyde uptake by the polyphenolic fraction, but it was too imprecise to take clear conclusions. In any case, measured ACRs are smaller than expected attending to oxygen consumption kinetics and acetaldehyde accumulation rates. No significant differences were found when comparing the acetaldehyde formed in situ or when acetaldehyde was spiked. 

Results show that oxygen consumed by wine is used to oxidize SO2, ethanol and at least 50 % to oxidize ascorbic acid, cysteine, glutathione, H2S, thiols, methionine and phenols. 

This work has been funded by the Spanish Ministry of Economy and Competitiveness (Spanish FPI Program AGL2014-59840-C2-1-R, AGL2017-59840), by Diputación General de Aragón (T53) and Fondo Social Europeo.

DOI:

Publication date: June 11, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Almudena Marrufo-Curtido, Elena Bueno-Aventín, Vicente Ferreira, Ana Escudero

Laboratory for Aroma analysis and Enology (LAAE). Instituto Agroalimentario de Aragón (ia2). Department of Analytical Chemistry. Associated unit to Instituto de Ciencias de la Vid y del Vino (ICVV-CSIC, UR, CAR) Universidad de Zaragoza.

Contact the author

Keywords

Oxygen, Acetaldehyde, Polyphenol index, Anthocyanins, flavonols, tannins and flavanol-anthocyanins adducts 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.