terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Limiting magnesium availability: a novel approach to managing brettanomyces spoilage in winemaking

Limiting magnesium availability: a novel approach to managing brettanomyces spoilage in winemaking

Abstract

Brettanomyces is a world-renowned yeast that negatively impacts the chemical composition of wines through the production of metabolites that negatively impact the sensory properties of the final product. Its resilience in wine conditions and ability to produce off-flavors make it a challenge for winemakers. Currently, the primary control technique involves adding sulfur dioxide (SO2); however, some Brettanomyces strains are developing resistance to this preservative agent. [1] Therefore, new management strategies are necessary to control this spoilage yeast.

 

Mineral nutrients could be a promising approach to control Brettanomyces growth in winemaking. Magnesium (Mg2+), is specifically recognized as a protective element against yeast stress and can prevent cellular death from temperature shock and ethanol. [2] In this study, the effect of mineral concentration on Brettanomyces metabolism under winemaking conditions was investigated. Brettanomyces sensitivity to Mg2+ limitation was assessed by culturing it in the presence of decreasing Mg2+ concentrations. While 1 mg/L of Mg2+ supported normal growth, concentrations below 0.5 mg/L significantly limited growth, and 0.1 mg/L was unable to support proliferation. Long-term Mg2+ deficiency resulted in permanent viability reduction.

 

This nutritional study identifies Mg2+ as essential for Brettanomyces metabolism and provides a starting point for designing experiments to manage Brettanomyces spoilage in wine, improving quality and stability. Understanding the physiology and nutritional requirements of Brettanomyces can lead to more informed decisions and innovative strategies for managing spoilage, resulting in better wine quality, consumer satisfaction, and higher profits for the wine industry. The study assists in finding alternatives to SO2 to produce fault-free wines that align with current wine consumer preferences. 

Acknowledgments:

The ARC Training Centre for Innovative Wine Production

The Australian Wine Research Institute

Pernod Ricard Winemakers

References:

1)  Bartel, C., et al. (2021). “Adaptive evolution of sulfite tolerance in Brettanomyces bruxellensis.” FEMS Yeast Research 21(5), https://doi.org/10.1093/femsyr/foab036.

2)  Birch, R. M. and G. M. Walker (2000). “Influence of magnesium ions on heat shock and ethanol stress responses of Saccharomyces cerevisiae.” Enzyme and Microbial Technology 26(9-10): 678-687, https://doi.org/10.1016/S0141-0229(00)00159-9.

 

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Giordano1-2, J. Macintyre3, A. Borneman1-4, P. Grbin1

1 Wine Science Discipline, School of Agriculture Food and Wine, Waite Research Institute, The University of Adelaide, Urrbrae, South Australia 5064, Australia
2 Australian Research Council Training Centre for Innovative Wine Production, The University of Adelaide, Urrbrae, South Australia 5064, Australia
3 Pernod Ricard Winemakers, 1914 Barossa Valley Way, Rowland Flat, SA 5352, Australia
4 The Australian Wine Research Institute, PO Box 197, Glen Osmond, Adelaide, SA 5064, Australia

Contact the author*

Keywords

Brettanomyces, wine spoilage, sulfur dioxide, mineral nutrients, magnesium, wine quality

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effects of heat and water stress on grapevine health: primary and secondary metabolism

Grapevine resilience to climate change has become one of the most pressing topics in the Viticulture & Enology field. Vineyard health demands understanding the mechanisms that explain the direct and indirect interactions between environmental stressors. The current climate change scenario, where drought and heat-wave are more frequent and intense, strongly demands improving our knowledge of environmental stresses. During a heatwave, the ambient temperature rises above the plant’s average tolerance threshold and, generally, above 35 oC plant’s adaptation to heat stress is activated.

Preliminary results of water status and metabolite content of three new crossbreed winegrape genotypes

This study presents the preliminary results obtained in 2022, of the evaluation of three new crossbreed winegrape genotypes and their parental varieties, grown under controlled irrigation (60% ETc) and rainfed conditions in a wine-growing area with scarcity of water and high temperatures (Murcia, southeast Spain). The genotypes MC16 and MC80 were obtained from crosses between the varieties ‘Monastrell’ and ‘Cabernet Sauvignon’, and MS104 from crosses between ‘Monastrell’ and ‘Syrah’ [1]. The objective of this study was to analyse the physiological response and vegetative development of the 6 genotypes under the two irrigation conditions, and to study their effect on the content of soluble sugars and chlorophyll in the leaf.

Toasting and grain effect on Tempranillo red wine aged in Quercus petraea barrels

The barrel-making process is widely recognized as a crucial practice that affects the composition of barrel-aged wine. After the drying process, the staves are considered ready for barrel assembly, which includes the processes of bending and toasting the barrel structure. Toasting is considered one of the most critical stages in determining the physical and chemical composition of the staves, which can influence the chemical and sensory composition of the wine aged in barrels made from them [1].

Implications of the nature of organic mulches used in vineyards on grapevine water status, yield, berry quality and biological soil health  

Climate emergency is going to affect the agricultural suistainability, wine grapes being probably one of the crops more sensitive to environmental constraints. In this context, mitigation strategies such as the revalorization of agricultural wastes are paramount to cope with the current challenges. The use of organic mulches has been reported to reduce soil water evaporation and improve vine water status, reduce soil erosion, and increase soil organic matter with little impact on berry quality. However, less is known about their effects on the microbiote of vineyards.

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character.