terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Abstract

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).

The cell wall fraction of strains from different yeast species were extracted by autolysis and alkali methods: Saccharomyces cerevisiae, Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima. The profiles of the polysaccharide fraction were analyzed by HPLC-DAD and HRSEC-RID. The protein and glycoprotein profiles were analyzed by SDS-PAGE. The effect on MLF of the addition of 2 g/L of each mannoprotein extract was evaluated in a wine like-medium using the O. oeni strain PSU-1 (ATCC BAA-331). The consumption of L-malic was monitored by an enzymatic method. The analysis of mannoprotein consumption, in terms of equivalents of mannose, was carried out by HPLC-MWC-RID.

The polysaccharide composition and the size of mannoproteins extracted by the two methods were significantly different for all the mannoproteins. Protein and glycoprotein profiles were also different in all the studied yeast walls. The addition of mannoprotein extract influenced the evolution of MLF differently according to the extraction method. Mannoproteins obtained by the yeast autolysis showed a positive effect on MLF in all cases; this effect was also observed in two S. cerevisiae and L. thermotolerans extracted by the alkali method. However, MLF was arrested after consuming 0.5 g/L of L-malic acid in the rest of fermentations with mannoproteins obtained by the alkali method. The results obtained indicate that the capacity of O. oeni to use mannoproteins depends on the mannoprotein composition, which in turns depends on the yeast species and the extraction method.

Keywords: Malolactic fermentation, mannoproteins, Oenococcus oeni

1) Chu-Ky S. et al. (2005). Biochimica et Biophysica Acta 1717, 118-124
2) Diez L. et al. (2010). Journal of Agricultural and Food Chemistry. 58, 7731–7739

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Paloma Toraño 1a*, María Oyón-Ardoiz 2, Elvira Manjón 2, Ignacio García-Estévez 2, Albert Bordons1a, Nicolas Rozès 1b, M. Teresa Escribano-Bailón2, Cristina Reguant 1a

1a Grupo de Biotecnología Enológica, 1bGrupo de Biotecnología Microbiana de los Alimentos, Departamento de Bioquímica y Biotecnología, Universitat Rovira i Virgili, Tarragona, España
2 Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, España

Contact the author*

Keywords

Malolactic fermentation, mannoproteins, Oenococcus oeni

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Aroma characterization of mold resistant base wines for sparkling wine produced in a warm-temperate area at two different altitudes

In a recent context where consumers pay an increasing attention to sustainability and eco-friendly aspects in the decision-making process, the use of the resistant varieties in the wine sector have returned to the attention. In this context, the use of mould-resistant grape varieties would be an opportunity for sparkling wine producers as it can reduced the pesticide utilization in grape management and hence production costs.
However, the use of the resistant varieties to produce the base wine may be strongly influenced due to its requirements for a particular balance between sugars and acidity to ensure the quality of the final product. In addition, the aromatic profile of base wine plays a crucial role in the perception of the quality of the sparkling wine.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

How are canned wine drinkers perceived? An investigation involving Swiss nationals and different scenarios of outdoor leisure activities

This study examines how people who consume wine in cans are perceived in terms of their basic personality characteristics, helps understand the role of cultural background on people’s perception, and verify the role played by the consumption context on the perception. Our hypothesis is that prejudice and negative attitudes towards wine in cans might exert a negative effect on the evaluation of people who consume canned wine. To evaluate this hypothesis, the consumption of wine in cans was evoked in four different contexts of use during outdoor leisure activity (beach resort, ski resort, desert safari, and party). In order to examine the effect of culture on subject’s response we use participants from Switzerland, a country where three different cultures, associated with three different languages, cohabit.

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

Prediction of aromatic attributes of red wines from its colour properties 

Wine perception is a multisensory experience that makes use of the sight, smell, and taste senses. When wine is sensorially assessed, the stimulus received generates multiple signals that tasters convert into organoleptic descriptors. Colour is commonly the first attribute evaluated during wine tasting. Moreover, the colour properties provide the taster with a priori information of the wine’s aroma. This preconceived perception is later confirmed or denied during the aroma evaluation.