Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities.

Vineyards from mild temperature, high humidity locations receive often treatments with fungicides to prevent damages produced by fungi responsible for mildium, oidium and botrytis infections. In addition, insecticides are also applied to vineyards to fight again pests, which affect directly, or indirectly (as vectors of different diseases), their productivity. A fraction of the above compounds reaches the soil of vineyards, either during application, or when released from the canopy of vines due to rain-wash-off. Thereafter, depending on soil conditions (pH, organic matter) and environmental variables (regimen of rain, slope of vineyards), they might persist in this compartment, be degraded and/or transferred to water masses, modifying the biodiversity of soils and/or affecting the quality of water reservoirs.