terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Abstract

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).

The cell wall fraction of strains from different yeast species were extracted by autolysis and alkali methods: Saccharomyces cerevisiae, Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima. The profiles of the polysaccharide fraction were analyzed by HPLC-DAD and HRSEC-RID. The protein and glycoprotein profiles were analyzed by SDS-PAGE. The effect on MLF of the addition of 2 g/L of each mannoprotein extract was evaluated in a wine like-medium using the O. oeni strain PSU-1 (ATCC BAA-331). The consumption of L-malic was monitored by an enzymatic method. The analysis of mannoprotein consumption, in terms of equivalents of mannose, was carried out by HPLC-MWC-RID.

The polysaccharide composition and the size of mannoproteins extracted by the two methods were significantly different for all the mannoproteins. Protein and glycoprotein profiles were also different in all the studied yeast walls. The addition of mannoprotein extract influenced the evolution of MLF differently according to the extraction method. Mannoproteins obtained by the yeast autolysis showed a positive effect on MLF in all cases; this effect was also observed in two S. cerevisiae and L. thermotolerans extracted by the alkali method. However, MLF was arrested after consuming 0.5 g/L of L-malic acid in the rest of fermentations with mannoproteins obtained by the alkali method. The results obtained indicate that the capacity of O. oeni to use mannoproteins depends on the mannoprotein composition, which in turns depends on the yeast species and the extraction method.

Keywords: Malolactic fermentation, mannoproteins, Oenococcus oeni

1) Chu-Ky S. et al. (2005). Biochimica et Biophysica Acta 1717, 118-124
2) Diez L. et al. (2010). Journal of Agricultural and Food Chemistry. 58, 7731–7739

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Paloma Toraño 1a*, María Oyón-Ardoiz 2, Elvira Manjón 2, Ignacio García-Estévez 2, Albert Bordons1a, Nicolas Rozès 1b, M. Teresa Escribano-Bailón2, Cristina Reguant 1a

1a Grupo de Biotecnología Enológica, 1bGrupo de Biotecnología Microbiana de los Alimentos, Departamento de Bioquímica y Biotecnología, Universitat Rovira i Virgili, Tarragona, España
2 Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, España

Contact the author*

Keywords

Malolactic fermentation, mannoproteins, Oenococcus oeni

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.

Identification of important genomic regions controlling resistance to biotic and abiotic stresses in Vitis sp. through QTL meta-analysis

In the context of global change, the environmental conditions are expected to be more stressful for viticulture. The choice of the rootstock may play a crucial role to improve the adaptation of viticulture to new biotic and abiotic threats (Ollat et al., 2016). However, the selection of interesting traits in rootstock breeding programs is complex because of the combination of multiple targets in a same ideotype. In this sense, the integration of studies about the genetic architecture for desired biotic and abiotic response traits allow us to identify genomic regions to combine and those with interesting pleiotropic effects.

Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

The use of grapevine genetic diversity is a way to mitigate the negative impacts of climate change on viticulture systems. Leaf epidermal flavonoids (including flavonols and anthocyanins) are involved in plant defense mechanisms against environmental stresses, like high temperatures or excessive solar radiation [1,2]. Among other factors, they modulate light absorption, which reduces photoinhibition processes in photosynthetic tissues [1]. Therefore, the identification of grapevine cultivars with an increased content on leaf epidermal flavonoids arises as a potential avenue to improve grapevine tolerance to some detrimental environmental stresses.

Retrospective analysis of our knowledge regarding the genetics of relevant traits for rootstock breeding 

Rootstocks were the first sustainable and environmentally friendly strategy to cope with a major threat for Vitis vinifera cultivation. In addition to providing Phylloxera resistance, they play an important role in protecting against other soil-borne pests, such as nematodes, and in adapting V. vinifera to limiting abiotic conditions. Today viticulture has to adapt to ongoing climate change whilst simultaneously reducing its environmental impact. In this context, rootstocks are a central element in the development of agro-ecological practices that increase adaptive potential with low external inputs. Despite the apparent diversity of the Vitis genus, only few rootstock varieties are used worldwide and most of them have a very narrow genetic background. This means that there is considerable scope to breed new, improved rootstocks to adapt viticulture for the future.

Extreme vintages affect grape varieties differently: a case study from a cool climate wine region

Eger wine region is located on the northern border of grapevine cultivation zone. In the cool climate, terroir selection is one of the foundations of quality wine making. However, climate change will have a significant impact on these high value-added vineyards. This study presents a case study from 2021 and 2022 with the investigation of three grape varieties (Kadarka, Syrah, Furmint). The experiment was conducted in a steep-sloped vineyard (Nagy-Eged hill) with a southern exposure.