terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Abstract

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).

The cell wall fraction of strains from different yeast species were extracted by autolysis and alkali methods: Saccharomyces cerevisiae, Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima. The profiles of the polysaccharide fraction were analyzed by HPLC-DAD and HRSEC-RID. The protein and glycoprotein profiles were analyzed by SDS-PAGE. The effect on MLF of the addition of 2 g/L of each mannoprotein extract was evaluated in a wine like-medium using the O. oeni strain PSU-1 (ATCC BAA-331). The consumption of L-malic was monitored by an enzymatic method. The analysis of mannoprotein consumption, in terms of equivalents of mannose, was carried out by HPLC-MWC-RID.

The polysaccharide composition and the size of mannoproteins extracted by the two methods were significantly different for all the mannoproteins. Protein and glycoprotein profiles were also different in all the studied yeast walls. The addition of mannoprotein extract influenced the evolution of MLF differently according to the extraction method. Mannoproteins obtained by the yeast autolysis showed a positive effect on MLF in all cases; this effect was also observed in two S. cerevisiae and L. thermotolerans extracted by the alkali method. However, MLF was arrested after consuming 0.5 g/L of L-malic acid in the rest of fermentations with mannoproteins obtained by the alkali method. The results obtained indicate that the capacity of O. oeni to use mannoproteins depends on the mannoprotein composition, which in turns depends on the yeast species and the extraction method.

Keywords: Malolactic fermentation, mannoproteins, Oenococcus oeni

1) Chu-Ky S. et al. (2005). Biochimica et Biophysica Acta 1717, 118-124
2) Diez L. et al. (2010). Journal of Agricultural and Food Chemistry. 58, 7731–7739

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Paloma Toraño 1a*, María Oyón-Ardoiz 2, Elvira Manjón 2, Ignacio García-Estévez 2, Albert Bordons1a, Nicolas Rozès 1b, M. Teresa Escribano-Bailón2, Cristina Reguant 1a

1a Grupo de Biotecnología Enológica, 1bGrupo de Biotecnología Microbiana de los Alimentos, Departamento de Bioquímica y Biotecnología, Universitat Rovira i Virgili, Tarragona, España
2 Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, España

Contact the author*

Keywords

Malolactic fermentation, mannoproteins, Oenococcus oeni

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

INTEGRAPE guidelines and tools: an effort of COST Action CA17111

INTEGRAPE was a European interdisciplinary network for “data integration to maximize the power of omics for grapevine improvement” (CA17111, https://integrape.eu/), funded by the European COST Association from September 2018 to 2022. This Action successfully developed guidelines and tools for data management and promoted the best practices in grapevine omics studies with a holistic future vision of: “Imagine having all data on grapevine accessible in a single place”.

Evaluation of physiological properties of grapevine clones of ‘Tempranillo’ and ‘Graciano’ in DOCa Rioja (Spain)

In order to avoid the loss of grapevine intra-varietal diversity of DOCa Rioja grape varieties, Regional Government of La Rioja established a germplasm bank with more than 1.600 accessions, whose origin lies in the prospecting and sampling of ancient vineyards located throughout the whole region. 30 clones of Tempranillo and 13 clones of Graciano were preselected and multiplied in a new vineyard for further observations. The aim of this work is to describe the first results from the physiological characterization by an optical sensor of these preselected clones, which constitute the base of a new clonal selection that aims to increase the range of available certified clones and to improve the adaptation of these varieties to future objectives and environmental conditions.

Unraveling the complexity of high-temperature tolerance by characterizing key players of heat stress response in grapevine

Grapevine (Vitis spp.) is greatly influenced by climatic conditions and its economic value is therefore directly linked to environmental factors. Among these factors, temperature plays a critical role in vine phenology and fruit composition. In such conditions, elucidating the mechanisms employed by the vine to cope with heat waves becomes urgent. For the past few years, our research team has been producing molecular and metabolic data to highlight the molecular players involved in the response of the vine and the fruit to high temperatures [1]. Some of these temperature-sensitive genes are currently undergoing characterization using transgenesis approaches coupled or not with genome editing, taking advantage of the Microvine genotype [2].

Towards the understanding of wine distillation in the production of brandy de Jerez. Chemical and sensory characterization of two distillation methods: continuous and batch distillation

Brandy de Jerez (BJ) is a spirit drink made exclusively from spirits and wine distillates and is characterized by the use of casks for aging that previously contained Sherries. The quality and sensory complexity of BJ depend on the raw materials and some factors: grape variety, conditions during processing the wine and its distillation, as well as the aging in the cask. Therefore, the original compounds of the grapes from which it comes are of great interest being in most cases the Airén variety. Their relationship with the quality of the musts and the wines obtained from them has been studied (1) and varies each year of harvest depending on the weather conditions (2).

Optimization of the ripening time of new varieties descendants of Monastrell

Given the impact of climate change on viticulture in the Region of Murcia, this paper attempts to expose the possibility of using genetic improvement as a dilemma that allows access to new descendant varieties of the autochthonous variety Monastrell crossed with varieties such as Syrah and Cabernet. Sauvignon, thus obtaining hybrids (Gebas and Myrtia). In it, the chromatic parameters and the phenolic profile of the new varieties will be compared with those obtained by the Monastrell variety at two moments during maturation (12 and 14 º Baumé), to check if the results would allow earlier harvests in these new varieties thus avoiding the decoupling between phenolic and technological maturity, while improving the quality of grapes and wines.