terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Abstract

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).

The cell wall fraction of strains from different yeast species were extracted by autolysis and alkali methods: Saccharomyces cerevisiae, Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima. The profiles of the polysaccharide fraction were analyzed by HPLC-DAD and HRSEC-RID. The protein and glycoprotein profiles were analyzed by SDS-PAGE. The effect on MLF of the addition of 2 g/L of each mannoprotein extract was evaluated in a wine like-medium using the O. oeni strain PSU-1 (ATCC BAA-331). The consumption of L-malic was monitored by an enzymatic method. The analysis of mannoprotein consumption, in terms of equivalents of mannose, was carried out by HPLC-MWC-RID.

The polysaccharide composition and the size of mannoproteins extracted by the two methods were significantly different for all the mannoproteins. Protein and glycoprotein profiles were also different in all the studied yeast walls. The addition of mannoprotein extract influenced the evolution of MLF differently according to the extraction method. Mannoproteins obtained by the yeast autolysis showed a positive effect on MLF in all cases; this effect was also observed in two S. cerevisiae and L. thermotolerans extracted by the alkali method. However, MLF was arrested after consuming 0.5 g/L of L-malic acid in the rest of fermentations with mannoproteins obtained by the alkali method. The results obtained indicate that the capacity of O. oeni to use mannoproteins depends on the mannoprotein composition, which in turns depends on the yeast species and the extraction method.

Keywords: Malolactic fermentation, mannoproteins, Oenococcus oeni

1) Chu-Ky S. et al. (2005). Biochimica et Biophysica Acta 1717, 118-124
2) Diez L. et al. (2010). Journal of Agricultural and Food Chemistry. 58, 7731–7739

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Paloma Toraño 1a*, María Oyón-Ardoiz 2, Elvira Manjón 2, Ignacio García-Estévez 2, Albert Bordons1a, Nicolas Rozès 1b, M. Teresa Escribano-Bailón2, Cristina Reguant 1a

1a Grupo de Biotecnología Enológica, 1bGrupo de Biotecnología Microbiana de los Alimentos, Departamento de Bioquímica y Biotecnología, Universitat Rovira i Virgili, Tarragona, España
2 Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, España

Contact the author*

Keywords

Malolactic fermentation, mannoproteins, Oenococcus oeni

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Impact of toasting and botanical origin on oak wood (Q. sp.) volatilome using untargeted GCxGC-ToFMS analysis

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).

Application of an in vitro digestion model to study the bioaccessibility and the effect of the intestinal microbiota on the red wine proanthocyanidins 

Proanthocyanidins are important phenolic fraction for wine quality, contributing to astringency, bitterness and color. Their metabolism begins in the mouth and continues throughout the gastrointestinal tract; however, most of them are accumulated in the colon where are metabolized by the intestinal microbiota, giving rise to a whole series of phenolic acids that may have greater activity at physiological level than the precursors[1]. This study aimed to evaluate in vitro the bioaccessibility of proanthocyanidins in a red wine developed by Bodegas Pradorey, as well as to evaluate the potential effect of intestinal microbiota on polyphenols metabolism identifying and quantifying secondary metabolites.

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.

Phenolic extraction and dissolved oxygen concentration during red wines fermentations with Airmixig M.I.™

During red wine fermentation, the extraction of phenolics compounds and sufficient oxygen provision are critical for wine quality [1,2]. In this trial, we aimed at evaluating the kinetics of phenolic extraction and dissolved oxygen during red wine fermentations using the airmixing system. Twenty lots of red grape musts were fermented in 300.000 L tanks, equipped with airmixing, using two injection regimes (i.e., high and low intensity, and high and low daily frequency). An oxygen analyzer was introduced into the tanks in order to record the concentration of dissolved oxygen over time.

Prediction of aromatic attributes of red wines from its colour properties 

Wine perception is a multisensory experience that makes use of the sight, smell, and taste senses. When wine is sensorially assessed, the stimulus received generates multiple signals that tasters convert into organoleptic descriptors. Colour is commonly the first attribute evaluated during wine tasting. Moreover, the colour properties provide the taster with a priori information of the wine’s aroma. This preconceived perception is later confirmed or denied during the aroma evaluation.