terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars


Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities. Grapevine leaves being a disposable byproduct in viticulture, their potential valorization as a source of polyphenols is a topic of interest.

Our objective was to compare the diversity of grapevine leaves composition by screening qualitatively and quantitatively the polyphenol content in leaves of 50 cultivars grown in the same field of an experimental collection at Bordeaux INRAe. Fresh leaves were collected at the same period in summer, freeze-dried, ground to a fine powder and polyphenols were extracted twice with combination of organic solvents (100% and 70% methanol). A targeted HPLC-MS/MS approach was used for the quantification with available standards of 60 different polyphenols.

The results showed high variability in polyphenols content. Nevertheless, caftaric acid and quercetin 3-glucuronide were the major compounds detected in all leaves. Flavanols, and more particularly the ratio catechin/epicatechin, could be explored as markers to determine leaf cultivar, ranging from 0.5 to 42. Stilbenes were minor compounds in all leaves, found mainly in the forms of trans- and cis-piceid. Minor presence of resveratrol and its oligomers was assessed by HPLC-HRMS/MS.

Acknowledgements: The authors wish to thank the UE Viticole and Louis Bordenave for management of the experimental vineyard and the support of Bordeaux Metabolome



(1) Lemaitre-Guillier et al (2021) VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. https://doi.org/10.3390/molecules26144258

(2) Goufo et al (2020). A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves. doi: 10.3390/antiox9050398


Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article


Alan Jamain1, Margot Larose1, Andreu Mairata2, Manon Delapena1, Antonio Palos-Pinto1, Céline Franc1, Maria Lafargue3, Ghislaine Hilbert-Masson3, Stéphanie Cluzet1, Josep Valls Fonayet1

1Enology, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France
2Instituto de Ciencias de la Vid y el Vino, 26007 Logroño (La Rioja) ESPAÑA
3EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33140 Villenave d’Ornon, France

Contact the author*


Vitis vinifera, Quercetin, Caftaric, Byproducts


2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series


Related articles…

Entomopathogenic nematodes application for controlling Lobesia botrana in grapevine and their impact on grapevine quality 

Entomopathogenic nematodes (EPN) are well-known biological control agents combined with specific adjuvants that now allow their use against aerial pests. Lobesia botrana (Lepidoptera: Tortricidae) is one of the major harmful pests detected in worldwide vineyards. Previous studies demonstrated that the EPNs Steinernema feltiae and S. carpocapsae could control L. botrana. The hypothesis was that the best combination of EPN-adjuvant/timing (season/temperatures) will support the use of EPN in the vineyard against L. botrana with no impact on the grape performance.

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

Survey of pesticide residues in vineyard soils from the Denomination of Origin Ribeiro

Vineyards from mild temperature, high humidity locations receive often treatments with fungicides to prevent damages produced by fungi responsible for mildium, oidium and botrytis infections. In addition, insecticides are also applied to vineyards to fight again pests, which affect directly, or indirectly (as vectors of different diseases), their productivity. A fraction of the above compounds reaches the soil of vineyards, either during application, or when released from the canopy of vines due to rain-wash-off. Thereafter, depending on soil conditions (pH, organic matter) and environmental variables (regimen of rain, slope of vineyards), they might persist in this compartment, be degraded and/or transferred to water masses, modifying the biodiversity of soils and/or affecting the quality of water reservoirs.

Analysis of the interaction of melatonin with glycolytic proteins in Saccharomyces cerevisiae during alcoholic fermentation 

Melatonin is a bioactive compound with antioxidant properties, that has been found in many fermented beverages, such as beer and wine [1]. Indeed, it has been shown that yeast can synthesize melatonin during alcoholic fermentation, although its role inside the cell, as well as the metabolic pathway involved in its synthesis, is still unclear [1]. Recent studies showed that during fermentation, melatonin interacts with different proteins of the glycolytic pathway in both Saccharomyces and non-Saccharomyces yeast, for instance glyceraldehyde 3-phosphate dehydrogenase, pyruvate kinase or enolase [2].

Polysaccharide families of lyophilized extracts obtained from unfermented varietal grape pomaces

The recovery of bioactive compounds from grape and wine by-products is currently an important objective for revaluation and sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds. The aim of this study was to evaluate the polysaccharide (PS) composition of extracts obtained from pomaces of different white and red grape varieties of Castilla y León. Grape pomaces were obtained after the pressing in the winemaking process.