terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Abstract

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis  mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum). A total of 43 different VOCs were detected in both varieties by gas chromatography coupled to mass spectrometry (SPME GC-MS). The quantitative analyses revealed that leaves of CS had higher concentration of VOCs than those of T, the effect of the mycorrhizal symbiosis on the total levels of VOCs being non-significant. The predominant VOCs were those synthetized by the LOX/HPL pathway, followed by those coming from MVA/MEP pathway and, in a lesser extent, from SK pathway. Pentyl leaf volatiles (PLV), green leaf volatiles (GLV) and VOCs involved in the resistance of grapevine against Plasmopara viticola were more abundant in CS than in T, especially when plants were associated with AMF. The volatilome profiles obtained revealed that some minor VOCs were only present in one of the two tested grapevine varieties. Mycorrhizal association increased the relative abundance (%) of VOCs derived from the SK pathway in T and that of GLV in CS.

Acknowledgements: To A. Urdiain, M. Oyarzun & H. Santesteban for technical support, Asociación de Amigos (UNAV) for D. Kozikova’s scholarship, Bioera SL for AMF, Ministerio de Ciencia e Innovación (Gobierno España) funded the research (Ref. PID2020-118337RB-IOO)

References:

1)  Velásquez A. et al. (2020) The arbuscular mycorrhizal fungus Funneliformis mosseae induces changes and increases the concentration of volatile organic compounds in Vitis vinifera cv. Sangiovese leaf tissue. Plant Physiol. Biochem. 155: 437-443, DOI 10.1016/j.plaphy.2020.06.048

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Goicoechea Nieves1*, Kozikova Daria1, Pascual Inmaculada1

1Environmental Biology department- Group of Stress Physiology in Plants. School of Sciences-BIOMA, University of Navarra. Irunlarrea 1, 31008-Pamplona, Spain

Contact the author*

Keywords

Cabernet Sauvignon, leaves, mycorrhizal symbiosis, Tempranillo, volatile organic compounds

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Applicability of grape native yeasts to enhance regional wine typicity

The universalization in wine production has been restricting the imprint of terroir in regional wines, resulting in loss of typicity. Microbes are the main driving force in wine production, conducting fermentation and originating a myriad of metabolites that underly wine aroma. Grape berries harbor an ecological niche composed of filamentous fungi, yeasts and bacteria, which are influenced by the ripening stage, cultivar and region. The research project GrapeMicrobiota gathers a consortium from University of Zaragoza, University of Minho and University of Tours and aims at the isolation of native yeast strains from berries of the wine region Douro, UNESCO World Heritage, towards the production of wines that stand out in the market for their authenticity and for reflecting their region of origin in their aroma.

Effect of riboflavin on the longevity of white and rosé wines

Light is a fundamental part at sales points which influences in the conservation of wines, particularly in those that are sold in transparent glass bottles such as rosé wines and increasingly white wines. The photochemical effect known as “light-struck taste” can cause changes in the aromatic characteristics of the wine. This “light-struck taste” is due to reactions triggered by the photochemical sensitivity of riboflavin (RBF).

Mapping grapevine metabolites in response to pathogen challenge: a Mass Spectrometry Imaging approach

Every year, viticulture is facing several outbreaks caused by established diseases, such as downy mildew and grey mould, which possess different life cycles and modes of infection. To cope with these different aggressors, grapevine must recognize them and arm itself with an arsenal of defense strategies.
The regulation of secondary metabolites is one of the first reactions of plants upon pathogen challenge. Their rapid biosynthesis can highly contribute to strengthen the defense mechanisms allowing the plant to adapt, defend and survive.

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.

Do wine sulphites affect gut microbiota? An in vitro study of their digestion in the gastrointestinal tract

“Sulphites” and mainly sulphur dioxide (SO2) is by far the most widely used additive (E-220/INS 220) in winemaking and likely the most difficult to replace. The well-known antioxidant, antioxidasic and antimicrobial properties of SO2 make this molecule a practically essential tool, not only in winemaking, but also in the production of other food products. The current trend in winemaking is the reduction of this unfriendly additive due to its negative effects on health and environmental. In particular, it could cause headaches and intolerance/allergic reactions in sensitive individuals. Wine is considered one of the major contributors of exposure of SO2 in the adult population, when this beverage is included in the diet.