terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Abstract

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis  mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum). A total of 43 different VOCs were detected in both varieties by gas chromatography coupled to mass spectrometry (SPME GC-MS). The quantitative analyses revealed that leaves of CS had higher concentration of VOCs than those of T, the effect of the mycorrhizal symbiosis on the total levels of VOCs being non-significant. The predominant VOCs were those synthetized by the LOX/HPL pathway, followed by those coming from MVA/MEP pathway and, in a lesser extent, from SK pathway. Pentyl leaf volatiles (PLV), green leaf volatiles (GLV) and VOCs involved in the resistance of grapevine against Plasmopara viticola were more abundant in CS than in T, especially when plants were associated with AMF. The volatilome profiles obtained revealed that some minor VOCs were only present in one of the two tested grapevine varieties. Mycorrhizal association increased the relative abundance (%) of VOCs derived from the SK pathway in T and that of GLV in CS.

Acknowledgements: To A. Urdiain, M. Oyarzun & H. Santesteban for technical support, Asociación de Amigos (UNAV) for D. Kozikova’s scholarship, Bioera SL for AMF, Ministerio de Ciencia e Innovación (Gobierno España) funded the research (Ref. PID2020-118337RB-IOO)

References:

1)  Velásquez A. et al. (2020) The arbuscular mycorrhizal fungus Funneliformis mosseae induces changes and increases the concentration of volatile organic compounds in Vitis vinifera cv. Sangiovese leaf tissue. Plant Physiol. Biochem. 155: 437-443, DOI 10.1016/j.plaphy.2020.06.048

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Goicoechea Nieves1*, Kozikova Daria1, Pascual Inmaculada1

1Environmental Biology department- Group of Stress Physiology in Plants. School of Sciences-BIOMA, University of Navarra. Irunlarrea 1, 31008-Pamplona, Spain

Contact the author*

Keywords

Cabernet Sauvignon, leaves, mycorrhizal symbiosis, Tempranillo, volatile organic compounds

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

Impact of climate on berry weight dynamics of a wide range of Vitis vinifera cultivars 

In order to study the impact of climate change on Bordeaux grape varieties and to assess the behavior of candidate grape varieties potentially better adapted to the new climatic conditions, an experimental vineyard composed of 52 grape varieties was planted in 2009 at the INRAE Bordeaux Aquitaine center[1]. Among the many parameters studied since 2012, berry weight for each variety was measured weekly from mid-veraison to maturity, with four independent replicates. The kinetics obtained allowed to study berry growth, a key parameter in grape composition and yield.

First results on the chemical composition of red wines from the pressing of marc

In the Bordeaux vineyards, press wine represents approximately 15% of the total volume of wine produced[1]. Valuing this large volume of wine is necessary from an economic point of view, but also because of their organoleptic contribution to the blend, and their contribution to the construction of wines for laying down. Therefore, this study was developed considering the lack of recent scientific knowledge on the composition of red press wines. The aim of this study is to establish an initial assessment of their chemical composition including aromatic compounds and a phenolic part.

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison.