terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic variation among wild grapes native to Japan

Genetic variation among wild grapes native to Japan

Abstract

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan.  A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan. Hence, the present study aimed to assess the ploidy level and genetic variability among the wild grapes native to Japan. A total of seven wild grape, in addition to two hybrids (Vitis vinifera and Vitis ficifolia (Japanese wild grape)) and one each Vitis vinifera and Vitis labruscana cultivars, were evaluated using 14 random amplified polymorphic DNA (RAPD) markers. The RAPD markers have been reported to be highly variable both within and between species. The RAPD markers were selected simply because of their uniqueness, simplicity and discriminatory capability. Likewise, ploidy level was determined by flow cytometric methods. The flow cytometric analysis showed no variation among wild grapes and their hybrids. All grapes were diploid irrespective of origin and diverse morphological, phenological and berry characteristics. The 14 RAPD primers amplified 120 reproducible bands among 11 grape accessions. Of the 120 total bands, 114 were polymorphic and 6 were monomorphic. The unweighted pair group method of arithmetic averages (UPGMA) using 120 RAPD bands from 14 selected primers clearly separated the wild grapes into distinct groups. The affinity of hybrid grapes with their parents proved true hybridity.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Puspa Raj Poudel1,2*, Ikuo Kataoka3, Kenji Beppu3 and Ryosuke Mochioka2

1Tribhuvan University, Institute of Agriculture and Animal Science, Paklihawa Campus, Siddharthanagar1, Rupandehi, Nepal
2University Farm, Faculty of Agriculture, Kagawa University, Showa, Sanuki, Kagawa 769-2304, Japan
3Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

Contact the author*

Keywords

wild grapes, RAPD, ploidy level, Vitis ficifolia

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effects of heat and water stress on grapevine health: primary and secondary metabolism

Grapevine resilience to climate change has become one of the most pressing topics in the Viticulture & Enology field. Vineyard health demands understanding the mechanisms that explain the direct and indirect interactions between environmental stressors. The current climate change scenario, where drought and heat-wave are more frequent and intense, strongly demands improving our knowledge of environmental stresses. During a heatwave, the ambient temperature rises above the plant’s average tolerance threshold and, generally, above 35 oC plant’s adaptation to heat stress is activated.

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock.

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum).

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Rootstocks not only provide tolerance to Phylloxera, but also ensure the supply of water and mineral nutrients to the whole plant. Rootstocks are an important way of adapting to environmental conditions while conserving the typical features of scion varieties. We can exploit the large diversity of rootstocks used worldwide to aid this adaptation. The aim of this study was to characterise rootstock regulation of scion mineral status and its relation with scion development.