terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic variation among wild grapes native to Japan

Genetic variation among wild grapes native to Japan

Abstract

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan.  A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan. Hence, the present study aimed to assess the ploidy level and genetic variability among the wild grapes native to Japan. A total of seven wild grape, in addition to two hybrids (Vitis vinifera and Vitis ficifolia (Japanese wild grape)) and one each Vitis vinifera and Vitis labruscana cultivars, were evaluated using 14 random amplified polymorphic DNA (RAPD) markers. The RAPD markers have been reported to be highly variable both within and between species. The RAPD markers were selected simply because of their uniqueness, simplicity and discriminatory capability. Likewise, ploidy level was determined by flow cytometric methods. The flow cytometric analysis showed no variation among wild grapes and their hybrids. All grapes were diploid irrespective of origin and diverse morphological, phenological and berry characteristics. The 14 RAPD primers amplified 120 reproducible bands among 11 grape accessions. Of the 120 total bands, 114 were polymorphic and 6 were monomorphic. The unweighted pair group method of arithmetic averages (UPGMA) using 120 RAPD bands from 14 selected primers clearly separated the wild grapes into distinct groups. The affinity of hybrid grapes with their parents proved true hybridity.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Puspa Raj Poudel1,2*, Ikuo Kataoka3, Kenji Beppu3 and Ryosuke Mochioka2

1Tribhuvan University, Institute of Agriculture and Animal Science, Paklihawa Campus, Siddharthanagar1, Rupandehi, Nepal
2University Farm, Faculty of Agriculture, Kagawa University, Showa, Sanuki, Kagawa 769-2304, Japan
3Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

Contact the author*

Keywords

wild grapes, RAPD, ploidy level, Vitis ficifolia

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Stomatal abundance in grapevine: developmental genes, genotypic variation, and physiology

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification.

Phenolic composition and chromatic characteristics of blends of cv. Tempranillo wines from vines grown with different viticultural techniques in a semi-arid area

The quality and color stability of red wines are directly related to content and distribution of phenolic compounds. However, the climate change produces the asynchrony between the dates of technological and maturity of grapes. The crop-forcing technique (CF) restores the coupling between phenolic and technological ripeness while limits vineyard yields. Blending of wines is frequently used to equilibriate composition of wines and to increase their stability, color and quality. The aim of the present work is to study the phenolic composition and color of wine blends made with FW (wines from vines subjected to CF) and CW (wines for vines under the usual cultivation practices).

Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Crops are facing increasing biotic and abiotic stress pressures due to global changes. However, trade-off mechanisms between these stresses and the underlying physiological processes are still poorly understood, especially in perennial crop species. To better understand these trade-offs, we studied the effect of drought on grapevine (Vitis vinifera) physiology and esca-related wood fungal communities. Esca is a vascular disease caused by a community of wood-infecting pathogenic fungi, and characterized by trunk necrosis, leaf scorch symptoms, yield losses, and mortality.

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

A phylogenomic study reveals the major dissemination routes of ‘Tempranillo Tinto’ in the Iberian Peninsula

‘Tempranillo Tinto’ is a black-berried Iberian cultivar that originated from a hybridization between cvs. ‘Benedicto’ and ‘Albillo Mayor’ [1]. Today, it is the third most widely grown wine grape cultivar worldwide with more than 200,000 hectares of vineyards mostly distributed along the Iberian Peninsula, where it is also known as ‘Cencibel’, ‘Tinta de Toro’, ‘Tinta Roriz’, and ‘Aragonez’, among other synonyms. Here, we quantified the intra-varietal genomic diversity in this cultivar through the study of 35 clones or ancient vines from seven different Iberian wine-making regions. A comparative analysis after Illumina whole-genome sequencing revealed the presence of 1,120 clonal single nucleotide variants (SNVs).