terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic variation among wild grapes native to Japan

Genetic variation among wild grapes native to Japan

Abstract

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan.  A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan. Hence, the present study aimed to assess the ploidy level and genetic variability among the wild grapes native to Japan. A total of seven wild grape, in addition to two hybrids (Vitis vinifera and Vitis ficifolia (Japanese wild grape)) and one each Vitis vinifera and Vitis labruscana cultivars, were evaluated using 14 random amplified polymorphic DNA (RAPD) markers. The RAPD markers have been reported to be highly variable both within and between species. The RAPD markers were selected simply because of their uniqueness, simplicity and discriminatory capability. Likewise, ploidy level was determined by flow cytometric methods. The flow cytometric analysis showed no variation among wild grapes and their hybrids. All grapes were diploid irrespective of origin and diverse morphological, phenological and berry characteristics. The 14 RAPD primers amplified 120 reproducible bands among 11 grape accessions. Of the 120 total bands, 114 were polymorphic and 6 were monomorphic. The unweighted pair group method of arithmetic averages (UPGMA) using 120 RAPD bands from 14 selected primers clearly separated the wild grapes into distinct groups. The affinity of hybrid grapes with their parents proved true hybridity.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Puspa Raj Poudel1,2*, Ikuo Kataoka3, Kenji Beppu3 and Ryosuke Mochioka2

1Tribhuvan University, Institute of Agriculture and Animal Science, Paklihawa Campus, Siddharthanagar1, Rupandehi, Nepal
2University Farm, Faculty of Agriculture, Kagawa University, Showa, Sanuki, Kagawa 769-2304, Japan
3Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

Contact the author*

Keywords

wild grapes, RAPD, ploidy level, Vitis ficifolia

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Selecting green cover species in the under-trellis zone of Lower Austrian vineyards

The under-trellis zone of vineyards is a sensitive area through which vines cover a significant portion of their nutrient and water needs. Mechanical and chemical methods are applied to suppress competing and tall-growing weeds to ensure optimal vine growth conditions. In addition to higher operating costs and depending on the soil conditions, these practices might lead to a long-term reduction in soil fertility and biodiversity. The presented study aims to analyse the suitability and interspecies competition of a selected green cover mixture of five local herbaceous species as potential green cover mixture in the under-trellis area of Lower Austrian vineyards.

Drought tolerance assessment and differentiation of grapevine cultivars using physiological metrics: insights from field studies

This study aimed to validate a protocol and compare metrics for evaluating drought tolerance in two Vitis vinifera grapevine cultivars under field conditions. Various metrics were calculated to represent the physiological responses of plants to progressive water deficit. Data were collected from Sauvignon Blanc and Chardonnay plants subjected to three irrigation levels during the 2022-2023 season, along with data from three previous seasons. Hydro-escape areas were used to assess the plant’s ability to reduce water potential with decreasing soil water availability.

Phenotyping bud break and trafficking of dormant buds from grafted vine

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1].

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

Detoxification capacities of heavy metals and pesticides by yeasts 

Winegrowing is still characterized by the extensive use of chemical fertilizers and plant protection products, despite strong recommendations to limit these practices. A part of these xenobiotics and metals are then found in grape juice and wine, causing a major health concern, as well as negatively affecting the fermentation process. In recent years, there has been renewed interest in non-Saccharomyces yeasts. These species have a wide phenotypic diversity, which would be exploited to broaden the aromatic palette of wines.