terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic variation among wild grapes native to Japan

Genetic variation among wild grapes native to Japan

Abstract

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan.  A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan. Hence, the present study aimed to assess the ploidy level and genetic variability among the wild grapes native to Japan. A total of seven wild grape, in addition to two hybrids (Vitis vinifera and Vitis ficifolia (Japanese wild grape)) and one each Vitis vinifera and Vitis labruscana cultivars, were evaluated using 14 random amplified polymorphic DNA (RAPD) markers. The RAPD markers have been reported to be highly variable both within and between species. The RAPD markers were selected simply because of their uniqueness, simplicity and discriminatory capability. Likewise, ploidy level was determined by flow cytometric methods. The flow cytometric analysis showed no variation among wild grapes and their hybrids. All grapes were diploid irrespective of origin and diverse morphological, phenological and berry characteristics. The 14 RAPD primers amplified 120 reproducible bands among 11 grape accessions. Of the 120 total bands, 114 were polymorphic and 6 were monomorphic. The unweighted pair group method of arithmetic averages (UPGMA) using 120 RAPD bands from 14 selected primers clearly separated the wild grapes into distinct groups. The affinity of hybrid grapes with their parents proved true hybridity.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Puspa Raj Poudel1,2*, Ikuo Kataoka3, Kenji Beppu3 and Ryosuke Mochioka2

1Tribhuvan University, Institute of Agriculture and Animal Science, Paklihawa Campus, Siddharthanagar1, Rupandehi, Nepal
2University Farm, Faculty of Agriculture, Kagawa University, Showa, Sanuki, Kagawa 769-2304, Japan
3Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

Contact the author*

Keywords

wild grapes, RAPD, ploidy level, Vitis ficifolia

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Vineyard yield estimation using image analysis: assessing bunch occlusions and its dependency on fruiting zone canopy features

Performing accurate vineyard yield estimation is of upmost importance as it provides important benefits to the whole vine and wine industry. Recently, image-analysis approaches have been explored to address this issue however this approach has as main challenge the bunch occlusion, mostly by vegetation but also by neighboring bunches. The present work aims at assessing the magnitude of bunch occlusion by neighboring bunches and to evaluate its dependency on a selection of vegetative and reproductive vine parameters assessed at fruiting zone. Forty vine segments (1 m) of two vineyard plots of the white cultivars ‘Alvarinho’ and ‘Arinto’ were assessed for vegetative and reproductive features at fruiting zone and imaged with a 2D camera.

Evaluation of physiological properties of grapevine clones of ‘Tempranillo’ and ‘Graciano’ in DOCa Rioja (Spain)

In order to avoid the loss of grapevine intra-varietal diversity of DOCa Rioja grape varieties, Regional Government of La Rioja established a germplasm bank with more than 1.600 accessions, whose origin lies in the prospecting and sampling of ancient vineyards located throughout the whole region. 30 clones of Tempranillo and 13 clones of Graciano were preselected and multiplied in a new vineyard for further observations. The aim of this work is to describe the first results from the physiological characterization by an optical sensor of these preselected clones, which constitute the base of a new clonal selection that aims to increase the range of available certified clones and to improve the adaptation of these varieties to future objectives and environmental conditions.

Sugar accumulation disorder Berry Shrivel – from current knowledge towards novel hypothesis

In contrast to fruit and grape berry ripening, the biological processes causing ripening disorders are often much less understood, although shriveling disorders of fruits are manifold and contribute to yield losses and reduced fruit quality worldwide. Shrinking berries are a common feature for all shriveling disorders in grapevine although their timing of appearance during the berry ripening process and their underlying induction processes distinct them from each other. The sugar accumulation disorder Berry Shrivel (BS) is characterized by a suppression of sugar accumulation short after veraison resulting in berries low in sugar content and anthocyanins in berry skins, while the organic acid content is similar. Recent studies analyzed the biochemical, morphological and molecular processes affected in BS berries and linked early changes to the period of ripening onset [1,2].

Effects of laccase from Botrytis cinerea on the oxidative degradation kinetics of the five natural grape anthocyanins

Enzymatic browning[1] is an oxidation process that occurs in many foods that increases the brown colour[2]. This problem is especially harmful in the wine industry[3]. especially when the grapes are infected by grey rot since this fung release the oxidative enzyme laccase[4]. In the particular case of red wines, the presence of laccase implies the deterioration of the red colour and can even cause the precipitation of the coloring matter (oxidasic haze)[5].