terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Characterization of non-cultivated wild grapevines in Extremadura (Spain) 

Characterization of non-cultivated wild grapevines in Extremadura (Spain) 

Abstract

Several Eurasian wild grapevine populations were found along Extremadura region (southwestern Spain). For conservation and study, one individual from four different populations (named L1, L2, L5 and L6) was vegetatively propagated and planted at Instituto de Investigaciones Agrarias Finca La Orden (CICYTEX), Badajoz. The aim of the present work was to characterize those conserved individuals from four different populations based on both an ampelographic description and a molecular analysis. Three vines per individual were studied. The ampelographic characterization was carried out according to 84 OIV descriptors from the list for grape varieties and Vitis species (2001). The genetic study, which includes a set of 13 microsatellite loci and 240 nuclear SNP data, was performed using total genomic DNA extracted from young leaves. Sexual organs (OIV 151) observed in L1 had fully developed stamens and reduced gynoecium (note 2) while L2, L5 and L6 were female (note 4). Attending berry size (OIV 220 and 221) two groups were found: (i) very short and narrow (1) in L5 and L6, and (ii) medium (3/5) in L2. Consequently, mean berry weight was larger in L2 (1.05 g) than in the other populations (< 0.5 g). Regarding the seeds (4 replicates of n = 100), their length was very short for all population except L2 (OIV 242). The wide/length coefficient of seeds was around 0.7 for L5 and L6 and significant lower in L2. The genetic study found four different genotypes. All of them were unique among the about 3000 genotypes existing in the ICVV-SNP database. The genetic structure analysis identified as Vitis vinifera subsp. sylvestris (Gmelin) (membership Q value > 0.9) the individuals from populations L5 and L6, while L2 corresponds most probably to Vitis vinifera subsp. vinifera (cultivated). Future studies on the evaluation of grapes and wines will help to better understand the potential oenological application of the wild grapevines characterized in this work.

Acknowledgements: We would like to express our gratitude to Miguel Lara (IFAPA, Rancho de la Merced, Jeréz de la Frontera, Cádiz, Spain) for the help with the ampelographic description.

 

This research was financially supported by Junta de Extremadura with European Regional Development Fund (ERDF) co-funding, through projects IB18102, GR21196 (Research Group AGA001), and AGROS at CICYTEX. L. Martín was supported by the DOC-INIA 2015 Program (Agencia Estatal de Investigación e Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Spain).

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Mª Teresa Guerra1, Mª Victoria Alarcón2, Francisco Vázquez2, Mª Esperanza Valdés3, Javier Ibáñez4, Laura Martín2*

1 Centro Universitario Santa Ana – Almendralejo (Badajoz). Spain
2 Instituto de Investigaciones Agrarias Finca La Orden-Valdesequera. Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX). Guadajira (Badajoz). Spain
3 Instituto Tecnológico Agroalimentario de Extremadura (CICYTEX). Badajoz. Spain
4 Instituto de Ciencias de la Vid y el Vino (ICVV). Logroño. Spain

Contact the author*

Keywords

ampelography, microsatellites, SNP, Vitis vinifera ssp. sylvestris (Gmelin) Heg

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Can yeast cells sense other yeasts beyond competition interactions?

The utilization of non-Saccharomyces yeasts in the wine industry has increased significantly in recent years. Alternative species need commonly be employed in combination with Saccharomyces cerevisiae to avoid stuck fermentation, or microbial spoilage. The employment of more than one yeast starter can lead to interactions between different species with an impact on the outcome of wine fermentation. Previous studies[1] demonstrated that S. cerevisiae elicits transcriptional responses with both shared and species-specific features in co-culture with other yeast species.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

Influence of irrigation frequency on berry phenolic composition of red grape varieties cultivated in four spanish wine-growing regions

The global warming phenomenon involves the frequency of extreme meteorological events accompanied by a change in rainfall distribution. Irrigation frequency (IF) affects the spatial and temporal soil water distribution but its effects on the phenolic composition of the grape have been scarcely studied. The aim of this work was to evaluate the effects of four deficit irrigation frequencies of 30 % ETo: one irrigation per day (T01), two irrigations per week (T03), one irrigation per week (T07) and one irrigation every two weeks (T15) on berry phenolic composition at harvest.

Identification of important genomic regions controlling resistance to biotic and abiotic stresses in Vitis sp. through QTL meta-analysis

In the context of global change, the environmental conditions are expected to be more stressful for viticulture. The choice of the rootstock may play a crucial role to improve the adaptation of viticulture to new biotic and abiotic threats (Ollat et al., 2016). However, the selection of interesting traits in rootstock breeding programs is complex because of the combination of multiple targets in a same ideotype. In this sense, the integration of studies about the genetic architecture for desired biotic and abiotic response traits allow us to identify genomic regions to combine and those with interesting pleiotropic effects.

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).