terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Abstract

Deleterious mutations that severely reduce population fitness are rapidly removed from the gene pool by purifying selection. However, evolutionary drivers such as genetic drift brought about by demographic bottlenecks may comprise its efficacy by allowing deleterious mutations to accumulate, thereby limiting the adaptive potential of populations. Moreover, positive selection can hitchhike mildly deleterious mutations due to linkage caused by lack of recombination. Similarly, in the context of species domestication, artificial selection mimics these evolutionary processes, which can have undesirable consequences for production and resilience. In this study, we evaluated the extent of the accumulation of deleterious mutations and the magnitude of their effects (also known as genetic load) at the whole-genome scale for ca. 500 grapevines including both wild Vitis species originated in three geographical regions (North America, Asia and Europe), and commercial varieties of V. vinifera ssp. sativa (using Illumina paired-end sequencing 20x from the 4k project[1]). We comparatively estimated the overall efficacy of selection as the ratio of non-synonymous to synonymous mutations in protein coding regions. We also performed various functional prediction analyses on the genomic sequence data to identify deleterious alleles and their effect on gene expression to quantify genetic load. In addition, 40 of the sequenced grapevine varieties were evaluated in an experimental common garden in Bordeaux (VitAdapt[2]). We obtained phenotypic traits related to wine productivity and resilience (e.g., phenology, biomass growth, water use efficiency and grape production) which allowed to analyse the correlation between the estimated genetic load and phenotypes. The results obtained from this work will contribute to the understanding of how we can best account for deleterious alleles and genetic load in new-generation grapevine breeding.

Acknowledgements: This study received financial support from the French government in the framework of the IdEX Bordeaux University “Investments for the Future” program / GPR Bordeaux Plant Sciences, and from the University of Bordeaux (PurVitis project).

1)  Dong, Y.,et al. (2023). Dual domestications and origin of traits in grapevine evolution. Science, 379(6635), 892901.

2)  Destrac Irvine A. and van Leeuwen C. (2016) The VitAdapt project: extensive phenotyping of a wide range of varieties in order to optimize the use of genetic diversity within the Vitis vinifera species as a tool for adaptation to a changing environment. Climwine, sustainable grape and wine production in the context of climate change, 11-13 April 2016, Bordeaux. Full text proceedings paper, 165-171.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Enrique Sáez-Laguna 1*, Gaëtan Craye 1Agnes Destrac Irvine 2, Cornelis van Leeuwen 2, Nabil Girollet 2, Pierre-François Bert 2, Nathalie Ollat 2, Santiago González-Martínez 1, Marina de Miguel 2

1 BIOGECO, Univ. Bordeaux, INRAE, 33610 Cestas, France
2 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France

Contact the author*

Keywords

deleterious mutations, genetic load, fitness, grape, variety, production

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

Physico-chemical properties of vine pruning residues with potential as enological additive

Grapes are one of the world’s primary fruit crops, and pruning activities generate high amounts of annual wood wastes [1]. These pruning shoots contain valuable phenolic compounds and could have numerous potential applications [1,2]. Consequently, the aim of this work was to evaluate the physico-chemical properties of vine pruning residues with potential as enological additives. For this purpose, grapevine shoots from 12 varieties grown in Chile were collected during the winter of 2021.

REGAVID a decision tool to deficit irrigation in a temperate climate (DO Monterrei – Spain)

In temperate climates, such as in the North of Spain, the use of irrigation in the vineyard has not been required, due to the usual rainfall from June to August. In some large vineyards, irrigation management has been carried out, based on occasional support irrigation, or for the application of nutrients (fertigation). Currently it is necessary to implement decision support models to manage irrigation water in real time and avoid misuse of a scarce resource. Moreover, quality standards must be achieved, as in the previous rainfed viticulture.

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.