terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Abstract

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake. Rooted woody cuttings of the rootstocks Fercal (V. berlandieri x V. vinifera) and Couderc 3309 (V. riparia x V. rupestris) were grown within a hydroponic system (Kick-Brauckmann, 7.5 L pots) filled with modified half-strength Hoagland solution under semi-controlled climatic glasshouse conditions in 2021. Plants were grown with or without FeNa(III)- EDTA, and with two  NO3/NH4+ ratios (100:0; 50:50). The results could differentiate iron deficiency effects, nitrogen form effects and rootstock effects. Interveinal chlorosis of young leaves appeared earlier on 3309C from the second week of treatment with NO3/NH4+ (100:0)/-Fe, while Fercal leaves showed less severe symptoms after four weeks of treatment, corresponding with chlorophyll concentrations lower by 75% for 3309C and 57% for Fercal. The treatment NO3/NH4+(50:50)/+Fe had significantly higher biomass compared with NO3/NH4+(100:0)/+Fe in both rootstocks while results with NO3/NH4+(100:0)/-Fe were lowest. Ferric chelate reductase (FCR) activity was specifically increased under iron deficiency in Fercal with both N combinations, whereas 3309C showed an increase in FCR activity just with NO3/NH4+ (50:50). These first results indicate that rootstocks differ in their preference on both the physiological and molecular level depending on the nitrogen form and in interaction with iron deficiency stress.

References:

1) Nasar, J. et al. (2022). Nitrogen fertilization coupled with iron foliar application improves the photosynthetic characteristics, photosynthetic nitrogen use efficiency, and the related enzymes of maize crops under different planting patterns. Front. Plant Sci. 13, 988055.
2) Yin, H. et al. (2020). Effect of ammonium and nitrate supplies on nitrogen and sucrose metabolism of cabernet sauvignon (Vitis vinifera cv.). J. Sci. Food Agric.100(14), 5239–5250. doi: 10.1002/jsfa.10574.

DOI:

Publication date: October 9, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Khalil, Sarhan1*; Griesser, Michaela1; Tomasi, Nicola2; Zanin, Laura2; Lodovici, Arianna2; Forneck, Astrid1

University of Natural Resources and Life Sciences, Vienna (BOKU), Institute of Viticulture and Pomology, Korad-Lorenz-Straße 24, 3430 Tulln an der Donau, Austria.
2 University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences, Via delle Scienze, 206 – 33100 – Udine, Italy.

Contact the author*

Keywords

grapevine, rootstock, nitrate, ammonium, iron, ferric chelate reductase

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The weak role of organic mulches in shaping bacterial communities in grapevine

The interest in sustainable and ecologic agricultural practices in grapevine has grown significantly in recent years in the context of ecological transition. Organic mulches are treatments that support the circular economy and positively affect the soil and the plant. They are an alternative to herbicides and other conventional practices since they may influence soil moisture, erosion, structure and weed control. However, their effects on the soil and must microbiota remain unknown.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Global warming and increased frequency and/or severity of drought events are among the most threatening consequences of climate change for agricultural crops. In response to drought, grapevine (as many other plants) exhibits osmotic adjustment through active accumulation of osmolytes which in turn shift the leaf turgor loss point (TLP) to more negative values, allowing to maintain stomata opened at lower water potentials1. We investigated the capacity of Pinot noir leaves to modulate their osmotic potential as a function of: (i) time (seasonal osmoregulation), (ii) growing temperatures, and (iii) drought events, to enhance comprehension of the resilience of grapevines in drought conditions. We performed trails under semi-controlled field conditions, and in two different greenhouse chambers (20/15 °C vs 25/20 °C day/night). For two consecutive vegetative seasons, grafted potted grapevines (Pinot noir/SO4) were subjected to two different water regimes for at least 30 days: well-watered (WW) and water deficit (WD).

Exploring the prevalence of esca-induced leaf symptoms in French vineyards and the role of climate: a national scale analysis

Esca, a severe trunk disease affecting vineyards, is caused by fungal pathogens that induce wood necrosis and decay, leaf symptoms, yield losses, and potentially a rapid death of the vine. The prevalence of this disease varies across years, regions, cultivars, and plot ages. Despite its significance in understanding and predicting dieback risk in different vineyards, the role of climate in trunk diseases remains a relatively unexplored research area. While some studies have demonstrated the impact of certain climatic conditions on the prevalence of the disease, they often focus on a limited number of plots and yield conflicting results.We conducted a statistical analysis, using a Bayesian approach on a national database comprising prevalence data of esca from over 500 different plots in France, spanning the years 2003 to 2022 and encompassing various cultivars.

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.