terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Development of a new method for detecting acetic acid bacteria in wine

Development of a new method for detecting acetic acid bacteria in wine

Abstract

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.

The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages. The aim of this study was to develop a liquid culture medium for the early detection of acetic acid bacteria based on olfactometry[2].

The culture medium was designed by modifying a previously existing one for the detection of acetic acid bacteria. For this purpose, the nutrient content was modified to enhance the development of these microorganisms, and their selectivity was increased. The efficacy of the medium was studied by seeding it with pure cultures and with mixtures of microorganisms of different species, and with wines artificially contaminated. Finally, the medium was validated by using it both in the laboratory and in wineries, with wines of different types and origins. It was possible to establish a correlation between the initial concentration of acetic acid bacteria and the time of appearance of detectable levels of acetic acid in the medium by olfactometry.

Acknowledgements: Laboratorios Dolmar Tentamus for the help provided

References

1)  Bartowsky E.J. et al. (2008) Acetic acid bacteria spoilage of bottled red wine. A review. Int. J. Food Microbiol., 125: 60-70, DOI: 10.1016/j.ijfoodmicro.2007.10.016

2)  Rodrigues N. et al. (2015) Development and use of a new medium to detect yeasts of the genera Dekkera/Brettanomyces. J. Appl. Microbiol., 90: 588-599, DOI: 10.1046/j.1365-2672.2001.01275.x.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

A. Parra1*, A. Ovejas1, L. González-Arenzana2, A.R. Gutiérrez2 and I. López-Alfaro2

1Laboratorio Dolmar Tentamus, Paraje Micalanda, 26221 Gimileo, La Rioja, Spain
2ICVV, Instituto de Ciencias de la Vid y del Vino (Universidad de La Rioja, Gobierno de La Rioja and CSIC), Finca La Grajera, Ctra. Burgos km 6, 26007 Logroño (La Rioja), Spain

Contact the author*

Keywords

acetic acid bacteria, acetic acid, olfactometry, wine

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

Cumulative effect of deficit irrigation and salinity on vine responses

Climate change is increasing water needs in most of the wine growing regions while reducing the availability and quality of water resources for irrigation. In this context, the sustainability of Mediterranean viticulture depends on grapevine responses to the combinations of water and salt stress. With this aim, this work studies the effects of deficit irrigation and salinity on the physiology of the Tempranillo cultivar (Vitis vinifera L.) grafted onto a drought and salinity tolerant rootstock (1103 Paulsen).

Implications of the nature of organic mulches used in vineyards on grapevine water status, yield, berry quality and biological soil health  

Climate emergency is going to affect the agricultural suistainability, wine grapes being probably one of the crops more sensitive to environmental constraints. In this context, mitigation strategies such as the revalorization of agricultural wastes are paramount to cope with the current challenges. The use of organic mulches has been reported to reduce soil water evaporation and improve vine water status, reduce soil erosion, and increase soil organic matter with little impact on berry quality. However, less is known about their effects on the microbiote of vineyards.

Perception, liking and emotional response of tropical fruit aromas in Chardonnay wines

Tropical fruit aromas in wines are thought to be important to wine consumers, although there is little research to confirm this statement. With so many wine styles available, it has become important to understand the qualities that are desirable to consumers and how to achieve those qualities. Thiols and esters are compounds that have been found to cause tropical fruit aromas in chardonnay (ref). Fermentation temperature gradients and skin contact were found to increase these compounds using micro scale fermentations. This work aimed to scale up these fermentations/operations to determine if the desired tropical fruit aromas could still be achieved and if there is a perceivable difference in tropical fruit aromas, liking, and emotional response in the wines at the consumer level.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.