terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Development of a new method for detecting acetic acid bacteria in wine

Development of a new method for detecting acetic acid bacteria in wine

Abstract

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.

The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages. The aim of this study was to develop a liquid culture medium for the early detection of acetic acid bacteria based on olfactometry[2].

The culture medium was designed by modifying a previously existing one for the detection of acetic acid bacteria. For this purpose, the nutrient content was modified to enhance the development of these microorganisms, and their selectivity was increased. The efficacy of the medium was studied by seeding it with pure cultures and with mixtures of microorganisms of different species, and with wines artificially contaminated. Finally, the medium was validated by using it both in the laboratory and in wineries, with wines of different types and origins. It was possible to establish a correlation between the initial concentration of acetic acid bacteria and the time of appearance of detectable levels of acetic acid in the medium by olfactometry.

Acknowledgements: Laboratorios Dolmar Tentamus for the help provided

References

1)  Bartowsky E.J. et al. (2008) Acetic acid bacteria spoilage of bottled red wine. A review. Int. J. Food Microbiol., 125: 60-70, DOI: 10.1016/j.ijfoodmicro.2007.10.016

2)  Rodrigues N. et al. (2015) Development and use of a new medium to detect yeasts of the genera Dekkera/Brettanomyces. J. Appl. Microbiol., 90: 588-599, DOI: 10.1046/j.1365-2672.2001.01275.x.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

A. Parra1*, A. Ovejas1, L. González-Arenzana2, A.R. Gutiérrez2 and I. López-Alfaro2

1Laboratorio Dolmar Tentamus, Paraje Micalanda, 26221 Gimileo, La Rioja, Spain
2ICVV, Instituto de Ciencias de la Vid y del Vino (Universidad de La Rioja, Gobierno de La Rioja and CSIC), Finca La Grajera, Ctra. Burgos km 6, 26007 Logroño (La Rioja), Spain

Contact the author*

Keywords

acetic acid bacteria, acetic acid, olfactometry, wine

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Genetic prospecting of rainfed viticulture in the region with the largest cultivated area in Chile

The Maule region hosts up to a third of the total area of vineyards in Chile, in an environment where ancient practices inherited from the colonial past coexist with modernity and dynamism that include technified irrigation and fine vines. In the dry land of Maule there is a viticulture that has subsisted with ancient vines and traditions transmitted over generations, and there is little clarity about the origin and classification of the Maule viticulture, giving rise to the use of different concepts as synonyms to describe the ancient, minority, patrimonial or Criollas vines. In order to characterize and protect the ancient material, we studied the genetic diversity of a territorial collection that covers 80% of the communes of the region, prioritizing plants established more than 40-60 years ago.

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines.

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.

Effect of rising atmospheric CO2 levels on grapevine yield and composition by the middle of the 21st century: what can we learn from the VineyardFACE?

Atmospheric CO2 levels have been rising continuously since the industrial revolution, affecting crop physiology, yield and quality of harvest products, and grapevine is no exception [1]. Most of previously reported studies used potted plants in controlled environments, and explored grapevine response to relatively high CO2 levels, 700 ppm or more. The vineyardFACE, established in Geisenheim in 2012, uses a free air carbon dioxide enrichment (FACE) system to simulate a moderate (ambient +20%) increase in atmospheric CO2 in a vineyard planted with cvs. Cabernet-Sauvignon and Riesling grafted on rootstock 161-49 Couderc and SO4, respectively.

Teinturier grapes: Valorization as a source of high-value compounds for the Chilean food industry

The agri-food industry is constantly searching for ingredients of high functional value, healthy and of natural origin. One species of particular interest is Vitis vinifera, due to its recognized antioxidant potential. Among the grape varieties, one group possesses these antioxidant compounds not only in the skin, but also in its pulp: Teinturier. The red grape has traditionally been used for color correction purposes in winemaking, however, its high antioxidant content transforms it into a raw material of high potential for new formulations of ingredients and foods for the health and wellness market.