terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

Abstract

Copper and SO2 tolerance are two well-studied phenotypic traits of Saccharomyces cerevisiae. The genetic bases of these traits are the allelic expansion at the CUP1 locus and reciprocal translocation at the SSU1 locus, respectively. Previous work identified a negative association between SO2 and copper tolerance in S. cerevisiae wine yeasts. To understand the genetic basis of copper sensitivity, we used bulk-segregant QTL analysis and identified genetic variation at the SSU1 locus as a causative factor. This was confirmed through reciprocal hemizygosity analysis in a strain with 20 copies of CUP1. Transcriptional and proteomic analysis revealed that over-expression of SSU1 didn’t suppress CUP1 expression or limit protein production. Instead, it induced sulfur limitation when exposed to copper.Furthermore, we observed that an SSU1 over-expressing strain became more sensitive to moderately elevated copper concentrations in sulfur-limited conditions, indicating a burden on the sulfate assimilation pathway. Over-expression of MET 3/14/16, genes upstream of H2S production in the sulfate assimilation pathway increased the production of SO2 and H2S but did not improve copper sensitivity in an SSU1 over-expressing background. We conclude that copper and SO2 tolerance are conditional traits in S. cerevisiae and provide evidence of the metabolic basis for their mutual exclusivity.

Acknowledgements: For genome sequencing the authors would like to thank the Ramaciotti Center for Genomics which is funded through Bioplatforms Australia Pty Ltd (BPA), a National Collaborative Research Infrastructure Strategy (NCRIS). Proteomic data acquisition was obtained with support of the Adelaide Proteomics Centre at The University of Adelaide, in partnership with the South Australian Health and Medical Research Institute Proteomics Core Facility

References:

1)  Onetto CA. et al. (2023). SO2 and copper tolerance exhibit an evolutionary trade-off in Saccharomyces cerevisiae. PLoS Genetics, 19(3), e1010692.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Cristobal Onetto1*, Dariusz Kutyna1, Radka Kolouchova1, Jane McCarthy1, Anthony Borneman1, Simon Schmidt1

1The Australian Wine Research Institute, Glen Osmond, South Australia, Australia

Contact the author*

Keywords

Saccharomyces cerevisiae, Copper tolerance, SO2 tolerance

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Rootstock effect on Cabernet Sauvignon aromatic and chemical composition

Grape quality potential for wine production is strongly influenced by environmental parameters and agronomic factors. Several studies underline the rootstock effect on scions vegetative growth and berry composition [1] with an impact on wine quality. Rootstocks are promising agronomic tools for climate change adaptation and in most grape-growing regions the potential diversity of rootstocks is not fully used and only a few genotypes are planted. Moreover, little is known about the effect of rootstock genetic variability on the aromatic composition in wines.

Read More

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].

Read More

Agronomic and oenological behavior of the minority Mandón variety on two rootstocks in the D.O. Arribes

A large population of vines of the Mandón minority red variety (synonymous with Garró) has been located in old vineyards of the D.O. Arribes (Zamora and Salamanca) to conserve and recover this minority variety. The wines made with this variety are characterized by their good structure and color, interesting harmony, an excellently low pH, with high acidity, as well as complex aromas of blue fruits and a marked and expressive minerality.

Read More

Dynamics of Saccharomyces cerevisiae population in spontaneous fermentations from Granxa D’Outeiro terroir (DOP Ribeiro, NW Spain)

Granxa D’Outeiro is a recovered ancient vineyard located in the heart of DOP Ribeiro, where traditional white grapevine varieties are growing under sustainable management. Spontaneous fermentations using grape must from Treixadura, Albariño, Lado, Godello, and Loureira varieties were carried out at experimental winery of Evega. Yeasts were isolated from must and at different stages of fermentation. Those colonies belonging to Saccharomyces cerevisiae were characterized at strain level by mDNA-RFLPs.

Read More

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.

Read More