terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Applicability of grape native yeasts to enhance regional wine typicity

Applicability of grape native yeasts to enhance regional wine typicity

Abstract

The universalization in wine production has been restricting the imprint of terroir in regional wines, resulting in loss of typicity. Microbes are the main driving force in wine production, conducting fermentation and originating a myriad of metabolites that underly wine aroma. Grape berries harbor an ecological niche composed of filamentous fungi, yeasts and bacteria, which are influenced by the ripening stage, cultivar and region. The research project GrapeMicrobiota gathers a consortium from University of Zaragoza, University of Minho and University of Tours and aims at the isolation of native yeast strains from berries of the wine region Douro, UNESCO World Heritage, towards the production of wines that stand out in the market for their authenticity and for reflecting their region of origin in their aroma. A catalogue of over 200 yeast isolates was obtained from berries of cv. Sousão, Touriga Nacional and Viosinho, following enrichment steps in selective medium[1], and Saccharomyces cerevisiae strains were selected to conduct fermentations in laboratory and in cellar conditions. The volatile profiles of young wines were analysed by GC-FID and SPE Ion-trap MS[2], and compared to those obtained in fermentations carried out by commercial strains used by local farmers. Results showed clear distinctions between volatile profiles of wines produced with native strains or with commercial strains, opening perspectives for the industrial use of native yeasts for enhancing regional wine aroma.

Acknowledgements: This work was supported by FCT project GrapeMicrobiota (PTDC/BAA-AGR/2691/2020), “Contrato-Programa” (UIDB/04050/2020), CoLAB VINES & WINES, Sogevinus S.A., Diputación General de Aragón (T29_23) and European Social Fund.

References:

1) Martins V. et al. (2022) A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro. OENO One, 56(3): 257-270, DOI 10.20870/oeno-one.2022.56.3.5462

2)  Martins V. et al. (2020) Vineyard calcium sprays shift the volatile profile of young red wine produced by induced and spontaneous fermentation. Food Res. Int., 131: 108983, DOI 10.1016/j.foodres.2020.108983

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Ricardo López1*, Viviana Martins2, Hernâni Gerós2

1Laboratory for Flavor Analysis and Enology (LAAE), Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA) Associate Unit to Instituto de las Ciencias de la Vid y del Vino (ICVV) (UR-CSIC-GR), E-50009 Zaragoza, Spain
2Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal

Contact the author*

Keywords

native yeasts, regional terroir, wine typicity

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

Investigation of cellulose nanofiber-based films used as a protective layer to reduce absorption of smoke phenols into wine grapes

Volatile phenols from wildfire smoke are absorbed by wine grapes, resulting in undesirable smoky and ashy sensory attributes in the affected wine.[1] Unfortunately the severity of wildfires is increasing, particularly when grapes are ripening on the vine. The unwanted flavors of the wine prompted a need for solutions to prevent the uptake of smoke compounds into wine grapes. Films using cellulose nanofibers as the coating forming matrix were developed as an innovative means to prevent smoke phenols from entering Pinot noir grapes. Different film formulations were tested by incorporating low methoxy pectin or chitosan.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Preliminary study of the influence of ripening on the polysaccharide content of different red grape varieties

Grape skin has a barrier and protective function in grapes. Cell wall of grape skins is mainly composed of polysaccharides such as pectins, celulloses and hemicelluloses and structural proteins. Terroir, variety and changes during ripening can affect the content of polysaccharides in grapes. The aim of this study was to evaluate the content of polysaccharides (PS) in grapes along the ripening process. Three red grape varieties were studied: Garnacha (G), Tempranillo (T) and Prieto Picudo (PP).

Implications of the nature of organic mulches used in vineyards on grapevine water status, yield, berry quality and biological soil health  

Climate emergency is going to affect the agricultural suistainability, wine grapes being probably one of the crops more sensitive to environmental constraints. In this context, mitigation strategies such as the revalorization of agricultural wastes are paramount to cope with the current challenges. The use of organic mulches has been reported to reduce soil water evaporation and improve vine water status, reduce soil erosion, and increase soil organic matter with little impact on berry quality. However, less is known about their effects on the microbiote of vineyards.