terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Tackling the 3D root system architecture of grapevines: a new phenotyping pipeline based on photogrammetry

Tackling the 3D root system architecture of grapevines: a new phenotyping pipeline based on photogrammetry

Abstract

Plant roots fulfil important functions as they are responsible for the acquisition of water and nutrients, for anchorage and stability, for interaction with symbionts and, in some cases, for the storage of carbohydrates. These functions are associated with the Root System Architecture (RSA, i.e. the form and the spatial arrangement of the roots in the soil). The RSA results from several biological processes (elongation, ramification, mortality…) genetically determined but with high structural plasticity. In grapevine, several factors can influence the RSA development (e.g. rootstock and scion genotypes, soil and plant management…). However, the effects of all these factors on the establishment of the RSA and associated functions (e.g. drought tolerance) have hardly ever been assessed. Such an assessment could help to improve the management of vineyards in our changing world. This lack of knowledge is mainly associated to methodological difficulties to characterize the RSA during grapevine development in the vineyard. To take up this challenge, we developed a new phenotyping pipeline, connecting photogrammetric data (produced by ArcheovisionProduction) with plant structures reconstruction software (PlantScan3D) and two packages dedicated to plant architecture analysis and visualisation (MTG and PlantGL from the OpenAlea platform). This new approach was developed on the uprooted root systems of two perennial species: grapevine and maritime pine. Their robustness was evaluated by comparing root traits estimated by this pipeline to root traits measured manually or estimated by a reference technique (semi-automated 3D digitizing, used on maritime pine root systems [1]). With this pipeline, we have planned to characterize the RSA of different rootstock genotypes, from different plantation types, soil management or water treatments, and at several developmental stages. All these data will be used to calibrate a functional-structural root model to facilitate the selection of plant material aimed to overcome the negative effects of climate change.

Acknowledgements: This work was financially supported by the Environmental Sciences department of the University of Bordeaux (“PROJETS EMERGENTS”).

References:
1)  Danjon, F. and Reubens, B. (2008) Assessing and analyzing 3D architecture of woody root systems, a review of methods and applications in tree and soil stability, resource acquisition and allocation. Plant and soil, 303, 1-34

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Clément Saint Cast1*, Céline Meredieu2, Jean-Pascal Tandonnet1, Nathalie Ollat1, Frédéric Boudon3,4, Raphaël Ségura2, Pascal Mora5, Frédéric Danjon2

1EGFV, University of Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France
2BioGeCo, University of Bordeaux, INRAE, 33610, Cestas, France
3AGAP Institut, University of Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France
4CIRAD, UMR AGAP Institut, F-34398 Montpellier, France
5Archéovision, University of Bordeaux Montaigne, 33000, Bordeaux, France

Contact the author*

Keywords

root system architecture, 3D phenotyping, Vitis sp., Pinus pinaster, photogrammetry, architectural analysis

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials.

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).

Analysis of the interaction of melatonin with glycolytic proteins in Saccharomyces cerevisiae during alcoholic fermentation 

Melatonin is a bioactive compound with antioxidant properties, that has been found in many fermented beverages, such as beer and wine [1]. Indeed, it has been shown that yeast can synthesize melatonin during alcoholic fermentation, although its role inside the cell, as well as the metabolic pathway involved in its synthesis, is still unclear [1]. Recent studies showed that during fermentation, melatonin interacts with different proteins of the glycolytic pathway in both Saccharomyces and non-Saccharomyces yeast, for instance glyceraldehyde 3-phosphate dehydrogenase, pyruvate kinase or enolase [2].

Oenococcus oeni clonal diversity in the carbonic maceration winemaking

This essay was aimed to describe the clonal diversity of Oenococcus oeni in the malolactic fermentation of the carbonic maceration (CM) winemaking. The free and the pressed liquids from CM were sampled and compared to the wine from a standard winemaking with previous destemming and crushing (DC) of grapes [1]. O. oeni strain typification was performed by PFGE as González-Arenzana et al. described (2014) [2]. Results showed that 13 genotypes, referred as to letters, were distinguished from the 49 isolated strains, meaning the genotype “a” the 27%, the “b” the 14%, the “c” the 12%, the “d and e” the 10 % each other, and the remaining ones less than the 8% each one.