terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Abstract

Red grape pomace can be an important source of polysaccharides, but currently they are little studied and even less with viable and environmental extraction processes (green extraction). These green techniques must be able to break the cell wall so that the compounds contained in the cells, including polysaccharides, are released and can have a great influence on extraction yields, the chemical structure of polysaccharides and applications in wines. Amongst the emerging green techniques most applied to the extraction of bioactive compounds, such as polysaccharides, high-power ultrasound (US) and enzyme-assisted extraction stand out. High power ultrasonic assisted extraction is based on the application of mechanical sound waves with frequencies between 20 kHz and 100 kHz inducing acoustic cavitation in a liquid medium, which causes fragmentation and formation of pores in the cells of the cell wall, and leads to increased extraction and diffusion of polysaccharides. While the use of enzymes causes the rupture of the cell walls, hydrolyzing them under optimal experimental conditions and releasing polysaccharides at lower temperatures, avoiding possible changes in the structure and bioactivity of the polysaccharides. Extraction combined with both techniques can increase the extraction yield of polysaccharides and/or reduce the extraction time. In this work, the variables of extractant liquid pH at three levels, US application time at three levels and application of enzymes before or after the US treatment on the polysaccharide extraction yield were studied. All the tests were carried out at 30 kHz, a red pomace/liquid ratio of 1.3 and with a dose of 0.6 ml/Hl of enzymes. In addition, the distribution of the molecular weights of the extracts obtained in the different tests was determined.

Acknowledgments: This research was funded by the Ministry of Science, Innovation and Universities from the Spanish Government and Feder Funds, grant number PID2021-123361OR-C22.

DOI:

Publication date: October 13, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Ekhiñe Garaigordobil1, Samuel Mateo Rogríguez1, Diego Canalejo1, Zhao Feng1, Mikel Landín Ross-Magahy1, Leticia Martínez-Lapuente1, Zenaida Guadalupe1, Silvia Pérez Magariño2, Belén Ayestarán1

1Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC), Spain
2Instituto Tecnológico Agrario de Castilla y León (ITACyL), Spain

Contact the author*

Keywords

pomace, polysaccharides, ultrasound, enzymes, extraction yield

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Preliminary study of the influence of ripening on the polysaccharide content of different red grape varieties

Grape skin has a barrier and protective function in grapes. Cell wall of grape skins is mainly composed of polysaccharides such as pectins, celulloses and hemicelluloses and structural proteins. Terroir, variety and changes during ripening can affect the content of polysaccharides in grapes. The aim of this study was to evaluate the content of polysaccharides (PS) in grapes along the ripening process. Three red grape varieties were studied: Garnacha (G), Tempranillo (T) and Prieto Picudo (PP).

The interplay between water deficit and nitrogen and potassium nutrition in Vitis vinifera L.

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Under-vine management effects on grapevine vegetative growth, gas exchange and rhizosphere microbial diversity

The use of cover crops under the vines might be an alternative to the use of herbicides or tillage, improving grapevine quality and soil characteristics. The aim of this research was to study the implications of different management strategies of the soil under the vines (herbicide, cultivation or cover crops) on grapevine growth, water and nutritional status, gas exchange parameters and belowground microbial communities.
The experimental design consisted in 4 treatments applied on 35L-potted Tempranillo vegetative grapevines with 10 replicates each grown in an open-top greenhouse in 2022 and 2023. Treatments included two cover crop species (Trifolium fragiferum and Bromus repens), herbicide (glyphosate al 36%) and an untreated control.