terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Abstract

Red grape pomace can be an important source of polysaccharides, but currently they are little studied and even less with viable and environmental extraction processes (green extraction). These green techniques must be able to break the cell wall so that the compounds contained in the cells, including polysaccharides, are released and can have a great influence on extraction yields, the chemical structure of polysaccharides and applications in wines. Amongst the emerging green techniques most applied to the extraction of bioactive compounds, such as polysaccharides, high-power ultrasound (US) and enzyme-assisted extraction stand out. High power ultrasonic assisted extraction is based on the application of mechanical sound waves with frequencies between 20 kHz and 100 kHz inducing acoustic cavitation in a liquid medium, which causes fragmentation and formation of pores in the cells of the cell wall, and leads to increased extraction and diffusion of polysaccharides. While the use of enzymes causes the rupture of the cell walls, hydrolyzing them under optimal experimental conditions and releasing polysaccharides at lower temperatures, avoiding possible changes in the structure and bioactivity of the polysaccharides. Extraction combined with both techniques can increase the extraction yield of polysaccharides and/or reduce the extraction time. In this work, the variables of extractant liquid pH at three levels, US application time at three levels and application of enzymes before or after the US treatment on the polysaccharide extraction yield were studied. All the tests were carried out at 30 kHz, a red pomace/liquid ratio of 1.3 and with a dose of 0.6 ml/Hl of enzymes. In addition, the distribution of the molecular weights of the extracts obtained in the different tests was determined.

Acknowledgments: This research was funded by the Ministry of Science, Innovation and Universities from the Spanish Government and Feder Funds, grant number PID2021-123361OR-C22.

DOI:

Publication date: October 13, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Ekhiñe Garaigordobil1, Samuel Mateo Rogríguez1, Diego Canalejo1, Zhao Feng1, Mikel Landín Ross-Magahy1, Leticia Martínez-Lapuente1, Zenaida Guadalupe1, Silvia Pérez Magariño2, Belén Ayestarán1

1Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC), Spain
2Instituto Tecnológico Agrario de Castilla y León (ITACyL), Spain

Contact the author*

Keywords

pomace, polysaccharides, ultrasound, enzymes, extraction yield

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Sparkling wines and atypical aging: investigating the risk of refermentation

Sparkling wine (SW) production entails a two-steps process where grape must undergoes a primary fermentation to produce a base wine (BW) which is then refermented to become a SW. This process allows for the development of a new physicochemical profile characterized by the presence of foam and a different organoleptic profile.

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements. In each condition, the same two wines were blind tasted: a wine previously defined as delicate – a Pinot Noir from Australia, and a wine known for its robust character – a Tannat from Uruguay.

Biotype diversity within the autochthonous ‘Bobal’ grapevine variety

Bobal is the second most widely grown Spanish red grape variety (54,165 has), mainly cultivated in the Valencian Community and especially, in Utiel-Requena region (about 67% of 34,000 has). In this study, agronomic and enological parameters were determined in 98 biotypes selected during 2018 and 2019 in more than 50 vineyards over 50 years-old in the Utiel-Requena region. Moreover, a multi-criteria approach considering temperature and rainfall (Fig. 1A), among other parameters, was made to establish three different zones within the region (Fig. 1B), where in the future the selected biotypes will evaluated. In fact, in 2020, 4 replicates and 12 vines per biotype were planted in an experimental vineyard to preserve this important intra-cultivar diversity.

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.