terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Preliminary study of extraction of polysaccharides from pomace by high powered ultrasonic combined with enzymes

Abstract

Red grape pomace can be an important source of polysaccharides, but currently they are little studied and even less with viable and environmental extraction processes (green extraction). These green techniques must be able to break the cell wall so that the compounds contained in the cells, including polysaccharides, are released and can have a great influence on extraction yields, the chemical structure of polysaccharides and applications in wines. Amongst the emerging green techniques most applied to the extraction of bioactive compounds, such as polysaccharides, high-power ultrasound (US) and enzyme-assisted extraction stand out. High power ultrasonic assisted extraction is based on the application of mechanical sound waves with frequencies between 20 kHz and 100 kHz inducing acoustic cavitation in a liquid medium, which causes fragmentation and formation of pores in the cells of the cell wall, and leads to increased extraction and diffusion of polysaccharides. While the use of enzymes causes the rupture of the cell walls, hydrolyzing them under optimal experimental conditions and releasing polysaccharides at lower temperatures, avoiding possible changes in the structure and bioactivity of the polysaccharides. Extraction combined with both techniques can increase the extraction yield of polysaccharides and/or reduce the extraction time. In this work, the variables of extractant liquid pH at three levels, US application time at three levels and application of enzymes before or after the US treatment on the polysaccharide extraction yield were studied. All the tests were carried out at 30 kHz, a red pomace/liquid ratio of 1.3 and with a dose of 0.6 ml/Hl of enzymes. In addition, the distribution of the molecular weights of the extracts obtained in the different tests was determined.

Acknowledgments: This research was funded by the Ministry of Science, Innovation and Universities from the Spanish Government and Feder Funds, grant number PID2021-123361OR-C22.

DOI:

Publication date: October 13, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Ekhiñe Garaigordobil1, Samuel Mateo Rogríguez1, Diego Canalejo1, Zhao Feng1, Mikel Landín Ross-Magahy1, Leticia Martínez-Lapuente1, Zenaida Guadalupe1, Silvia Pérez Magariño2, Belén Ayestarán1

1Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC), Spain
2Instituto Tecnológico Agrario de Castilla y León (ITACyL), Spain

Contact the author*

Keywords

pomace, polysaccharides, ultrasound, enzymes, extraction yield

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Characterization of non-cultivated wild grapevines in Extremadura (Spain) 

Several Eurasian wild grapevine populations were found along Extremadura region (southwestern Spain). For conservation and study, one individual from four different populations (named L1, L2, L5 and L6) was vegetatively propagated and planted at Instituto de Investigaciones Agrarias Finca La Orden (CICYTEX), Badajoz. The aim of the present work was to characterize those conserved individuals from four different populations based on both an ampelographic description and a molecular analysis. Three vines per individual were studied.

Under-vine management effects on grapevine vegetative growth, gas exchange and rhizosphere microbial diversity

The use of cover crops under the vines might be an alternative to the use of herbicides or tillage, improving grapevine quality and soil characteristics. The aim of this research was to study the implications of different management strategies of the soil under the vines (herbicide, cultivation or cover crops) on grapevine growth, water and nutritional status, gas exchange parameters and belowground microbial communities.
The experimental design consisted in 4 treatments applied on 35L-potted Tempranillo vegetative grapevines with 10 replicates each grown in an open-top greenhouse in 2022 and 2023. Treatments included two cover crop species (Trifolium fragiferum and Bromus repens), herbicide (glyphosate al 36%) and an untreated control.

First results on the chemical composition of red wines from the pressing of marc

In the Bordeaux vineyards, press wine represents approximately 15% of the total volume of wine produced[1]. Valuing this large volume of wine is necessary from an economic point of view, but also because of their organoleptic contribution to the blend, and their contribution to the construction of wines for laying down. Therefore, this study was developed considering the lack of recent scientific knowledge on the composition of red press wines. The aim of this study is to establish an initial assessment of their chemical composition including aromatic compounds and a phenolic part.

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.

Toasting and grain effect on Tempranillo red wine aged in Quercus petraea barrels

The barrel-making process is widely recognized as a crucial practice that affects the composition of barrel-aged wine. After the drying process, the staves are considered ready for barrel assembly, which includes the processes of bending and toasting the barrel structure. Toasting is considered one of the most critical stages in determining the physical and chemical composition of the staves, which can influence the chemical and sensory composition of the wine aged in barrels made from them [1].