terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Abstract

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region. ED was compared to a control non-defoliated (ND). Berry temperature was continuously monitored and normal heat hours (NHH) were calculated. Photosynthetic active radiation at cluster level (PARcluster) was monitored at five phenological stages (green berry (GB), pea size (PS), veraison (VER), mid-ripening (MR) and full maturation (FM). Various berry parameters were monitored: sugars, acidity, wax content, berry permeance, flavonoid compounds, abscisic acid (ABA) and related metabolites. As compared to ND, ED induced ~80% increase in PARcluster, and higher NHH. Consequently, accumulated temperatures above 35ºC were higher in ED than in ND. No differences in anthocyanin compounds were observed at FM, however, in ED the glucoside forms of anthocyanins reached their maximum concentration at MR. A high correlation was found between anthocyanins and NHH (r>0.83, p<0.01) as well as between flavonols and PARcluster (r=0.73, p<0.05). ABA was slightly higher in ND than in ED for the same NHH and after VER, ABA decreased faster in ED than in ND. ABA-GE increased exponentially from VER, reaching its maximum at MR in ND, while in ED it continued to accumulate through FM. Neither the wax content nor the cuticle permeance were affected by the ED treatment. Overall, ED induced changes in cluster-zone thermal and light microclimate which impacted berry ripening metabolism.

Acknowledgements: This research received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013), grant agreement nº 311775, Project Innovine.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Olfa Zarrouk1,2, Ricardo Egipto2,3, Carla Pinheiro4, Cecilia Brunetti5,6, Antonella Gori6, Massimiliano Tattini5, M. Manuela Chaves1, Carlos M. Lopes2

1 Plant Molecular Ecophysiology Laboratory. Instituto de Tecnologia Química e Biológica (ITQB), Universidade NOVA de Lisboa, Oeiras, Portugal
2 LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
3 INIAV – Instituto Nacional de Investigação Agrária e Veterinária, Polo de Inovação de Dois Portos, 2565-191 Dois Portos, Portugal
4 Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
5 IPSP – Institute for Sustainable Plant Protection, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino (Florence), Italy
6 DAGRI – Department of Agriculture, Environment, Food and Forestry, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino (Florence), Italy

Contact the author*

Keywords

ABA metabolism, anthocyanins, flavonols,
normal heat hours, Vitis vinifera, waxes

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Global warming and increased frequency and/or severity of drought events are among the most threatening consequences of climate change for agricultural crops. In response to drought, grapevine (as many other plants) exhibits osmotic adjustment through active accumulation of osmolytes which in turn shift the leaf turgor loss point (TLP) to more negative values, allowing to maintain stomata opened at lower water potentials1. We investigated the capacity of Pinot noir leaves to modulate their osmotic potential as a function of: (i) time (seasonal osmoregulation), (ii) growing temperatures, and (iii) drought events, to enhance comprehension of the resilience of grapevines in drought conditions. We performed trails under semi-controlled field conditions, and in two different greenhouse chambers (20/15 °C vs 25/20 °C day/night). For two consecutive vegetative seasons, grafted potted grapevines (Pinot noir/SO4) were subjected to two different water regimes for at least 30 days: well-watered (WW) and water deficit (WD).

Quantifying water use diversity across grapevine rootstock-scion combinations

Vines require proper light levels, temperature, and water availability, and climate change is modifying these factors, hampering yield and quality. Despite the large diversity of rootstocks, varieties, and clones, we still lack knowledge of their combined effects and potential role in a warmer and dryer future. Therefore, we aim to characterize some of the existing diversity of rootstocks and genotypes and their interaction at the eco-physiological level, combining stomatal conductance (gs) and chlorophyll a fluorescence analysis.

Wine odors: chemicals, physicochemical and perceptive processes involved in their perception

The odors of wines are diverse, complex and dynamic and much research has been devoted to the understanding of their chemical bases. However, while the “basic” chemical part of the problem, namely the identity of the chemicals responsible for the different odor nuances, was satisfactorily solved years ago, there are some relevant questions precluding a clear understanding. These questions are related to the physicochemical interactions determining the effective volatilities of the odorants and, particularly, to the perceptual interactions between different odor molecules affecting in different ways to the final sensory outputs.

The 1000 grapevine genomes project: Cataloguing Australia’s grapevine germplasm

Grapevine cultivars can be unequivocally typed by both physical differences (ampelography) and genetic tests. However due to their very similar characteristics, the identification of clones within a cultivar relies on the accurate tracing of supply records to the point of origin. Such records are not always available or reliable, particularly for older accessions. Whole genome sequencing (WGS) provides the most highly detailed methodology for defining grapevine cultivars and more importantly, this can be extended to differentiating clones within those cultivars.

Ultra-High Pressure Homogenization (UHPH): a technique that allows the reduction of SO2 in winemaking

Ultra-High Pressure Homogenization (UHPH) is an innovative, efficient and non-thermal technology that can be applied at different stages in winemaking in order to reduce or avoid the use of sulphites. During 2022 vintage, a batch of Xarel·lo must was processed by UHPH at 300 MPa with an inlet temperature (Ti) of 4 ºC. In order to verify the influence of the UHPH treatment in wine characteristics, alcoholic fermentations with this must (UHPH) were carried out and compared with a control batch (without SO2 addition (C)) and a sulphited batch, in which 60 mg/L of total SO2 (SO2) were added.