terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Abstract

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region. ED was compared to a control non-defoliated (ND). Berry temperature was continuously monitored and normal heat hours (NHH) were calculated. Photosynthetic active radiation at cluster level (PARcluster) was monitored at five phenological stages (green berry (GB), pea size (PS), veraison (VER), mid-ripening (MR) and full maturation (FM). Various berry parameters were monitored: sugars, acidity, wax content, berry permeance, flavonoid compounds, abscisic acid (ABA) and related metabolites. As compared to ND, ED induced ~80% increase in PARcluster, and higher NHH. Consequently, accumulated temperatures above 35ºC were higher in ED than in ND. No differences in anthocyanin compounds were observed at FM, however, in ED the glucoside forms of anthocyanins reached their maximum concentration at MR. A high correlation was found between anthocyanins and NHH (r>0.83, p<0.01) as well as between flavonols and PARcluster (r=0.73, p<0.05). ABA was slightly higher in ND than in ED for the same NHH and after VER, ABA decreased faster in ED than in ND. ABA-GE increased exponentially from VER, reaching its maximum at MR in ND, while in ED it continued to accumulate through FM. Neither the wax content nor the cuticle permeance were affected by the ED treatment. Overall, ED induced changes in cluster-zone thermal and light microclimate which impacted berry ripening metabolism.

Acknowledgements: This research received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013), grant agreement nº 311775, Project Innovine.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Olfa Zarrouk1,2, Ricardo Egipto2,3, Carla Pinheiro4, Cecilia Brunetti5,6, Antonella Gori6, Massimiliano Tattini5, M. Manuela Chaves1, Carlos M. Lopes2

1 Plant Molecular Ecophysiology Laboratory. Instituto de Tecnologia Química e Biológica (ITQB), Universidade NOVA de Lisboa, Oeiras, Portugal
2 LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
3 INIAV – Instituto Nacional de Investigação Agrária e Veterinária, Polo de Inovação de Dois Portos, 2565-191 Dois Portos, Portugal
4 Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
5 IPSP – Institute for Sustainable Plant Protection, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino (Florence), Italy
6 DAGRI – Department of Agriculture, Environment, Food and Forestry, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino (Florence), Italy

Contact the author*

Keywords

ABA metabolism, anthocyanins, flavonols,
normal heat hours, Vitis vinifera, waxes

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.

Optimization of the acquisition of NIR spectrum in grape must and wine 

The characterization of chemical compounds related with quality of grape must and wine is relevant for the viticulture and enology fields. Analytical methods used for these analyses require expensive instrumentation as well as a long sample preparation processes and the use of chemical solvents. On the other hand, near-infrared (NIR) spectroscopy technique is a simple, fast and non-destructive method for the detection of chemical composition showing a fingerprint of the sample. It has been reported the potential of NIR spectroscopy to measure some enological parameters such as alcohol content, pH, organic acids, glycerol, reducing sugars and phenolic compounds.

Vineyard management practices to reduce sugar content on ‘Monastrell’ grapes

Climate change is resulting in more dry and hot summers, accelerating grape ripening and increasing berry sugars concentration. This results in wines with a higher alcohol content, which has a negative impact on wine quality, as well as, on consumer health. Agronomic practices that minimize these effects on berry composition and, consequently, on wine quality must be defined. In this work, different management practices have been assessed on rainfed ‘Monastrell’ grapevines in Jumilla (Murcia, Spain) from 2021 to 2023 vintages. Mulching, shading, application of kaolin and different types of pruning were evaluated, among others field adaptation practices.

Phenolic composition of Cabernet Sauvignon wines from Argentina, Portugal and Spain

Grape and wine phenolic compounds have been shown to be highly related to both wine quality (color, flavor, and taste) and health-promoting properties (antioxidant and cardioprotective, among others). The aim of this work was to evaluate and compare the phenolic contents of Cabernet Sauvignon wines from different geographical areas and climatic conditions, namely from Argentina, Portugal and Spain vintage 2022. In addition, the phenolic profiles of the Portuguese wines from three vintages (2020, 2021, 2022) was compared.