terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Abstract

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region. ED was compared to a control non-defoliated (ND). Berry temperature was continuously monitored and normal heat hours (NHH) were calculated. Photosynthetic active radiation at cluster level (PARcluster) was monitored at five phenological stages (green berry (GB), pea size (PS), veraison (VER), mid-ripening (MR) and full maturation (FM). Various berry parameters were monitored: sugars, acidity, wax content, berry permeance, flavonoid compounds, abscisic acid (ABA) and related metabolites. As compared to ND, ED induced ~80% increase in PARcluster, and higher NHH. Consequently, accumulated temperatures above 35ºC were higher in ED than in ND. No differences in anthocyanin compounds were observed at FM, however, in ED the glucoside forms of anthocyanins reached their maximum concentration at MR. A high correlation was found between anthocyanins and NHH (r>0.83, p<0.01) as well as between flavonols and PARcluster (r=0.73, p<0.05). ABA was slightly higher in ND than in ED for the same NHH and after VER, ABA decreased faster in ED than in ND. ABA-GE increased exponentially from VER, reaching its maximum at MR in ND, while in ED it continued to accumulate through FM. Neither the wax content nor the cuticle permeance were affected by the ED treatment. Overall, ED induced changes in cluster-zone thermal and light microclimate which impacted berry ripening metabolism.

Acknowledgements: This research received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013), grant agreement nº 311775, Project Innovine.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Olfa Zarrouk1,2, Ricardo Egipto2,3, Carla Pinheiro4, Cecilia Brunetti5,6, Antonella Gori6, Massimiliano Tattini5, M. Manuela Chaves1, Carlos M. Lopes2

1 Plant Molecular Ecophysiology Laboratory. Instituto de Tecnologia Química e Biológica (ITQB), Universidade NOVA de Lisboa, Oeiras, Portugal
2 LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
3 INIAV – Instituto Nacional de Investigação Agrária e Veterinária, Polo de Inovação de Dois Portos, 2565-191 Dois Portos, Portugal
4 Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
5 IPSP – Institute for Sustainable Plant Protection, National Research Council (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino (Florence), Italy
6 DAGRI – Department of Agriculture, Environment, Food and Forestry, University of Florence, Viale delle Idee 30, 50019 Sesto Fiorentino (Florence), Italy

Contact the author*

Keywords

ABA metabolism, anthocyanins, flavonols,
normal heat hours, Vitis vinifera, waxes

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Predicting provenance and grapevine cultivar implementing machine learning on vineyard soil microbiome data: implications in grapevine breeding

The plant rhizosphere microbial communities are an essential component of plant microbiota, which is crucial for sustaining the production of healthy crops. The main drivers of the composition of such communities are the growing environment and the planted genotype. Recent viticulture studies focus on understanding the effects of these factors on soil microbial composition since microbial biodiversity is an important determinant of plant phenotype, and of wine’s organoleptic properties. Microbial biodiversity of different wine regions, for instance, is an important determinant of wine terroir.

Understanding the impact of rising temperatures due to climate change on aromatic compositions in Malbec wines from Mendoza, Argentina

Mendoza is one of Argentina’s most important and outstanding wine regions producing the renowned Malbec wines due to its optimal soil and weather conditions. However, the effects of 21st-century climate change would negatively impact Malbec wines quality. This study investigated the effect of temperature increase and the impact of plant hormone abscisic acid (ABA) used to mitigate the negative effect of temperature increase on Malbec wines aromatic composition through GC-MS. Four treatments were applied on vines at field condition: Control, Control + 3 ºC, ABA and ABA + 3 ºC.

New crossbreed winegrape genotypes cultivated under rainfed conditions in a semi-arid Mediterranean region

Traditional drought tolerant varieties such as Cabernet Sauvignon, Monastrell, and Syrah [1], have been used as parents in the grapevine breeding program initiated by the Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA) in 1997 [2]. This work presents the results of evaluating three new genotypes obtained from crosses between ‘Monastrell’ and ‘Cabernet Sauvignon’ (MC16 and MC80) and between ‘Monastrell’ and ‘Syrah’ (MS104), comparing their performance under conditions of water scarcity and high temperatures with that of their respective parental varieties. For this purpose, the six genotypes were cultivated under controlled irrigation conditions (60% ETc) and rainfed conditions.

Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

The use of grapevine genetic diversity is a way to mitigate the negative impacts of climate change on viticulture systems. Leaf epidermal flavonoids (including flavonols and anthocyanins) are involved in plant defense mechanisms against environmental stresses, like high temperatures or excessive solar radiation [1,2]. Among other factors, they modulate light absorption, which reduces photoinhibition processes in photosynthetic tissues [1]. Therefore, the identification of grapevine cultivars with an increased content on leaf epidermal flavonoids arises as a potential avenue to improve grapevine tolerance to some detrimental environmental stresses.

Toasting and grain effect on Tempranillo red wine aged in Quercus petraea barrels

The barrel-making process is widely recognized as a crucial practice that affects the composition of barrel-aged wine. After the drying process, the staves are considered ready for barrel assembly, which includes the processes of bending and toasting the barrel structure. Toasting is considered one of the most critical stages in determining the physical and chemical composition of the staves, which can influence the chemical and sensory composition of the wine aged in barrels made from them [1].