terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Abstract

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied. Forty-two grape samples from different vineyards of Pago de Carraovejas Winery, were harvested at the optimum ripening. Polyamines and amino acids of these musts were analyzed by reverse-phase high performance liquid chromatography with a diode-array detection system. Enological parameters were also determined according to official analysis methods. Multifactor analysis (MFA) was performed using the RStudio program, considering stress as qualitative variable.

MFA allowed differentiating the samples by stress and grape variety. Grapes from more stressed vineyards had the highest content of arginine, alanine, glutamine, methionine, lysine and serine. In contrast, Cabernet Sauvignon and Merlot grapes showed higher content of proline, glycine, putrescine and spermidine, and lower content of most amino acids (tryptophan, glutamic acid, aspartic acid, arginine, alanine, glutamine and methionine) than Tempranillo grapes.

These results indicate influence of grape variety and stress factor on polyamine and amino acid content of grape berries. Polyamine concentration seems to be more associated to grape variety than to stress. Further studies are needed to assess the evolution of these compounds during ripening and fermentation since amino acids are an important source of nitrogen for yeast growth and they are volatile compound precursors.

Acknowledgements: This study was supported by the project 2022/474 from “Rural Development Program (PDR) of Castilla y León 2014-2020” and financed with FEADER funds.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Silvia Pérez-Magariño1*, Inés Sampedro-Marigómez1, Estela Cano-Mozo1, Clara Albors2, Lorena López2, Eva Navascués2

1 Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos Km 119, 47071 Valladolid, Spain.
2 Pago de Carraovejas, Camino de Carraovejas, s/n. 47300 Peñafiel, Valladolid, Spain.

Contact the author*

Keywords

environmental stress, amino acids, polyamines, grape varieties

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Options to replace or reduce the sulphite content in Tannat red wines produced with minimal intervention

Several Uruguayan wineries have begun to produce wines with minimal intervention, to increase the sustainability of their vineyards and wines. These wines are characterized by the minimum intervention in the management of the vineyard, its harvest, vinification, conservation and aging1,2. Sulfur dioxide (SO2) is not used or is used in reduced doses, although chitosan can be substituted or supplemented1. The objective of this research is to evaluate SO2 reduction or replacement options adapted to the production of Tannat red wines with minimal intervention. Vinification of the Tannat grapes with autochthonous yeasts (LN) was carried out during the 2023 vintage.

Viticultural heritage in mountain territories of Catalonia: prospecting in the region of Osona, northern Spain

The recovery of ancestral or minority vine varieties has been gaining great interest in recent years, among other reasons because it is likely that some of these varieties, due to the fact that they are found in relict areas, have a greater potential for adaptation to external factors (biotic or abiotic) and can minimize the effects that climate change is causing in viticulture. Varieties that can be grown at altitude are currently being sought to combat rising temperatures and prolonged extreme drought conditions. In Catalonia, the Pyrenean expansion of vineyard cultivation is documented from the 10th century and has been related to the “small climatic optimum” (9th-12th centuries) and also to seigniorial power.[1] But different adverse climatic periods and the arrival of Phylloxera by the late 19th century made many of these crops disappear.[2]

REGAVID a decision tool to deficit irrigation in a temperate climate (DO Monterrei – Spain)

In temperate climates, such as in the North of Spain, the use of irrigation in the vineyard has not been required, due to the usual rainfall from June to August. In some large vineyards, irrigation management has been carried out, based on occasional support irrigation, or for the application of nutrients (fertigation). Currently it is necessary to implement decision support models to manage irrigation water in real time and avoid misuse of a scarce resource. Moreover, quality standards must be achieved, as in the previous rainfed viticulture.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Rootstocks not only provide tolerance to Phylloxera, but also ensure the supply of water and mineral nutrients to the whole plant. Rootstocks are an important way of adapting to environmental conditions while conserving the typical features of scion varieties. We can exploit the large diversity of rootstocks used worldwide to aid this adaptation. The aim of this study was to characterise rootstock regulation of scion mineral status and its relation with scion development.

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.