terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Abstract

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied. Forty-two grape samples from different vineyards of Pago de Carraovejas Winery, were harvested at the optimum ripening. Polyamines and amino acids of these musts were analyzed by reverse-phase high performance liquid chromatography with a diode-array detection system. Enological parameters were also determined according to official analysis methods. Multifactor analysis (MFA) was performed using the RStudio program, considering stress as qualitative variable.

MFA allowed differentiating the samples by stress and grape variety. Grapes from more stressed vineyards had the highest content of arginine, alanine, glutamine, methionine, lysine and serine. In contrast, Cabernet Sauvignon and Merlot grapes showed higher content of proline, glycine, putrescine and spermidine, and lower content of most amino acids (tryptophan, glutamic acid, aspartic acid, arginine, alanine, glutamine and methionine) than Tempranillo grapes.

These results indicate influence of grape variety and stress factor on polyamine and amino acid content of grape berries. Polyamine concentration seems to be more associated to grape variety than to stress. Further studies are needed to assess the evolution of these compounds during ripening and fermentation since amino acids are an important source of nitrogen for yeast growth and they are volatile compound precursors.

Acknowledgements: This study was supported by the project 2022/474 from “Rural Development Program (PDR) of Castilla y León 2014-2020” and financed with FEADER funds.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Silvia Pérez-Magariño1*, Inés Sampedro-Marigómez1, Estela Cano-Mozo1, Clara Albors2, Lorena López2, Eva Navascués2

1 Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos Km 119, 47071 Valladolid, Spain.
2 Pago de Carraovejas, Camino de Carraovejas, s/n. 47300 Peñafiel, Valladolid, Spain.

Contact the author*

Keywords

environmental stress, amino acids, polyamines, grape varieties

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

High-throughput sequencing analysis based on nematode indices revealed healthier soils of organic vineyards 

Proper soil health assessments are crucial for sustainable cropland. Among the widely employed approaches, evaluating nematode community structure is particularly suitable. Traditionally, the taxonomic characterization of soil nematodes has relied on time-consuming morphology-based methods requiring experienced experts. However, molecular tools like high-throughput sequencing have emerged as efficient alternatives. In this study, we performed a metataxonomic analysis of soil samples collected from 57 vineyards in the DOCa Rioja region of Northern Spain, focusing on the impact of organic viticulture and cover cropping compared to integrated pest management (IPM) and tilling practices.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Rootstocks not only provide tolerance to Phylloxera, but also ensure the supply of water and mineral nutrients to the whole plant. Rootstocks are an important way of adapting to environmental conditions while conserving the typical features of scion varieties. We can exploit the large diversity of rootstocks used worldwide to aid this adaptation. The aim of this study was to characterise rootstock regulation of scion mineral status and its relation with scion development.

Dynamics of Saccharomyces cerevisiae population in spontaneous fermentations from Granxa D’Outeiro terroir (DOP Ribeiro, NW Spain)

Granxa D’Outeiro is a recovered ancient vineyard located in the heart of DOP Ribeiro, where traditional white grapevine varieties are growing under sustainable management. Spontaneous fermentations using grape must from Treixadura, Albariño, Lado, Godello, and Loureira varieties were carried out at experimental winery of Evega. Yeasts were isolated from must and at different stages of fermentation. Those colonies belonging to Saccharomyces cerevisiae were characterized at strain level by mDNA-RFLPs.

Unveiling a hidden link: does time hold the key to altered spectral signatures of grapevines under drought?

Remote sensing technology captures spectral data beyond the visible range, making it useful for monitoring plant stress. Vis-NIR (Visible-Near Infrared) spectroscopy (400-1000 nm) is commonly used to indirectly assess plant status during drought. One example is the widespread use of normalized difference vegetation index (NDVI) that is strongly linked to green biomass. However, a knowledge gap exists regarding the applicability of this method to all the drought conditions and if it is a direct correlation to the water status of the plant.

The use of plasma activated water in barrel disinfection: impact on oak wood composition

The use of barrels is a practice that improves the quality of wines. The porous structure of wood favors the accumulation of microorganisms that can deteriorate the quality of wines so that barrel cleaning and sanitizing treatments are essential. The burning of sulphur discs has been the most common practice in winemaking because ots biocide effect. Nevertheless, its effectiveness is still insufficient and it is harmful for human health.