terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Abstract

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied. Forty-two grape samples from different vineyards of Pago de Carraovejas Winery, were harvested at the optimum ripening. Polyamines and amino acids of these musts were analyzed by reverse-phase high performance liquid chromatography with a diode-array detection system. Enological parameters were also determined according to official analysis methods. Multifactor analysis (MFA) was performed using the RStudio program, considering stress as qualitative variable.

MFA allowed differentiating the samples by stress and grape variety. Grapes from more stressed vineyards had the highest content of arginine, alanine, glutamine, methionine, lysine and serine. In contrast, Cabernet Sauvignon and Merlot grapes showed higher content of proline, glycine, putrescine and spermidine, and lower content of most amino acids (tryptophan, glutamic acid, aspartic acid, arginine, alanine, glutamine and methionine) than Tempranillo grapes.

These results indicate influence of grape variety and stress factor on polyamine and amino acid content of grape berries. Polyamine concentration seems to be more associated to grape variety than to stress. Further studies are needed to assess the evolution of these compounds during ripening and fermentation since amino acids are an important source of nitrogen for yeast growth and they are volatile compound precursors.

Acknowledgements: This study was supported by the project 2022/474 from “Rural Development Program (PDR) of Castilla y León 2014-2020” and financed with FEADER funds.

DOI:

Publication date: October 16, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Silvia Pérez-Magariño1*, Inés Sampedro-Marigómez1, Estela Cano-Mozo1, Clara Albors2, Lorena López2, Eva Navascués2

1 Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos Km 119, 47071 Valladolid, Spain.
2 Pago de Carraovejas, Camino de Carraovejas, s/n. 47300 Peñafiel, Valladolid, Spain.

Contact the author*

Keywords

environmental stress, amino acids, polyamines, grape varieties

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Dynamics of Saccharomyces cerevisiae population in spontaneous fermentations from Granxa D’Outeiro terroir (DOP Ribeiro, NW Spain)

Granxa D’Outeiro is a recovered ancient vineyard located in the heart of DOP Ribeiro, where traditional white grapevine varieties are growing under sustainable management. Spontaneous fermentations using grape must from Treixadura, Albariño, Lado, Godello, and Loureira varieties were carried out at experimental winery of Evega. Yeasts were isolated from must and at different stages of fermentation. Those colonies belonging to Saccharomyces cerevisiae were characterized at strain level by mDNA-RFLPs.

Effects of laccase from Botrytis cinerea on the oxidative degradation kinetics of the five natural grape anthocyanins

Enzymatic browning[1] is an oxidation process that occurs in many foods that increases the brown colour[2]. This problem is especially harmful in the wine industry[3]. especially when the grapes are infected by grey rot since this fung release the oxidative enzyme laccase[4]. In the particular case of red wines, the presence of laccase implies the deterioration of the red colour and can even cause the precipitation of the coloring matter (oxidasic haze)[5].

Ecophysiological characterisation of terroir effects on Vitis vinifera L. Chardonnay and pinot noir in south african cool climate regions

Terroir encompasses environmental (climate, geology, soil and topography), genetic (cultivar and clone) and human factors (oenological and viticultural practices). Climate change brings about shifts in the suitability of a region for the growth of specific grapevine cultivars. This study focused on climatic and fruit parameters (berry size, weight, pH, total acidity (TA) and phenolics) to characterise the terroir effect in Vitis vinifera L. cultivars Chardonnay and Pinot Noir vineyards in the Cape South Coast region (Walker Bay and Elgin).

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum).