terclim by ICS banner
IVES 9 IVES Conference Series 9 Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Abstract

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species diversity of many of the relevant ecological niches within the broader wine environment, from vineyard soils to plants and grapes to fermentation. The data highlight that species identities and diversity significantly impact agronomic performance of vineyards as well as wine quality, but the complexity of these systems and of microbial growth dynamics has defeated attempts to offer actionable tools to guide or predict specific outcomes of ecosystem-based interventions. The application of such tools in future will depend on our understanding of the physiological and molecular drivers that govern microbial ecosystems. Here we describe several integrated approaches to characterize the molecular interactions between species within the fermentation and the waste-water ecosystem and to model the development of these ecosystems. Binary (two species) and consortia-based approaches indicate ecosystem-specific developmental patterns in these systems. On a molecular level, data strongly support that cell-wall related properties of yeast species impact the development of fermentation ecosystems during wine making and highlight the importance of physical contacts between species in these ecological processes. To model the wine yeast fermentation ecosystem, high-throughput flow cytometry-based approaches were developed, and specific models based on a machine-learning approach were developed. In winery wastewater, laboratory-based evolution of two species exposed to biotic selection pressure in a synthetic environment, Saccharomyces cerevisiae and the microalga Chlorella sorokiniana, identified two specific genes involved in carbon and nitrogen catabolite repression that facilitate mutualistic behaviors between yeast and microalgae when inactive. Taken together the data suggest novel strategies for microbial ecosystem-based decision making in wine making and improved integration of natural microbial biodiversity in the process.

DOI:

Publication date: February 11, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Florian F. Bauer, Cleo Conacher, Jennifer Oosthuizen, Georgia Strydom, Evodia Setati, Rene Naidoo-Blassoples

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch, South Africa

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

IMPACT OF ACIDIFICATION AT BOTTLING BY FUMARIC ACID ON RED WINE AFTER 2 YEARS

Global warming is responsible for a lack of organic acid in grape berries, leading to wines with higher pH and lower titrable acidity. The chemical, microbiological and organoleptic equilibriums are impacted by this change of organic acid concentration. It is common practice to acidify the wine in order to prevent these imbalances that can lead to wine defects and early spoilage. Tartaric acid (TA) is most commonly used by winemaker for wine acidification purposes. Fumaric acid (FA), which is authorized by the OIV in its member states for the inhibition of malolactic fermentation, could also be used as a potential acidification candidate since it has a better acidifying power than tartaric acid.

NEW PLANT BIOPOLYMERS FOR THE COLLOIDAL STABILITY OF THE COLORING MATTER OF RED WINES

The color as well as the “clarity” of red wines are ones of the qualities required by the consumers. Red wines must have colloidal stability from its bottling to its consumption. The supplementation of red wines with additives, and especially Acacia senegal gum, contributes to its organoleptic properties such as the colloidal stabilization of the coloring matter. In a global perspective of limitation of additives in the field of enology, one of the objectives is notably (i) to reduce the use of additives in wines, by their number and/or their quantity, and (ii) to favor the use of natural additives while preserving the organoleptic and sensory qualities of wines.

WINE SWIRLING: A FIRST STEP TOWARDS THE UNLOCKING OF THE WINE’STASTER GESTURE

Right after the pouring of wine in a glass, a myriad of volatile organic compounds, including ethanol, overwhelm the glass headspace, thus causing the so-called wine’s bouquet [1]. Otherwise, it is worth noting that during wine tasting, most people automatically swirl their glass to enhance the release of aromas in the glass headspace [1]. About a decade ago, Swiss researchers revealed the complex fluid mechanics underlying wine swirling [2]. However, despite mechanically repeated throughout wine tasting, the consequences of glass swirling on the chemical space found in the headspace of wine glasses are still barely known.

WHAT’S FUTURE FOR SANTORINI’S VITICULTURE IN THE CONTEXT OF CLIMATE CHANGE

The own-rooted vineyard of Santorini is a unique case of vineyard worldwide that is been cultivated for thousands of years. On the island’s volcanic soil, the vines are still cultivated with traditional techniques, which are adapted to the specific and extreme weather conditions that prevail on it. While climate change is a reality in the Mediterranean region, will Santorini vineyard endure its impact? The study of the traditional training systems, techniques and vine density, as well as the application of sustainable solutions (cover crops and use of kaolin etc.) revealed sustainable methods for the adaptation of the local viticulture to new climatic phenomena that tend to be more and more frequent in the region due to climate change.

WHICH IMPACT FOR PROANTHOCYANIDIC TANNINS ON RED WINE FRUITY AROMA? SENSORY AND PHYSICOCHEMICAL APPROACHES

Previous research on the fruity character of red wines highlighted the role of esters. Literature provides evidence that, besides these esters, other compounds that are not necessarily volatiles may have an important impact on the overall aroma of wine, contributing to a modulation of its global aromatic expression. The goal of this work was to assess the olfactory consequences of a mixture between esters and proanthocyanidic tannins, through sensory and physico-chemical approaches.
Sensory analysis of numerous aromatic reconstitutions, including triangular tests, detection thresholds, and sensory profiles, were conducted in order to evaluate the sensory impact of tannins on red wine esters perception.