terclim by ICS banner
IVES 9 IVES Conference Series 9 Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Abstract

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species diversity of many of the relevant ecological niches within the broader wine environment, from vineyard soils to plants and grapes to fermentation. The data highlight that species identities and diversity significantly impact agronomic performance of vineyards as well as wine quality, but the complexity of these systems and of microbial growth dynamics has defeated attempts to offer actionable tools to guide or predict specific outcomes of ecosystem-based interventions. The application of such tools in future will depend on our understanding of the physiological and molecular drivers that govern microbial ecosystems. Here we describe several integrated approaches to characterize the molecular interactions between species within the fermentation and the waste-water ecosystem and to model the development of these ecosystems. Binary (two species) and consortia-based approaches indicate ecosystem-specific developmental patterns in these systems. On a molecular level, data strongly support that cell-wall related properties of yeast species impact the development of fermentation ecosystems during wine making and highlight the importance of physical contacts between species in these ecological processes. To model the wine yeast fermentation ecosystem, high-throughput flow cytometry-based approaches were developed, and specific models based on a machine-learning approach were developed. In winery wastewater, laboratory-based evolution of two species exposed to biotic selection pressure in a synthetic environment, Saccharomyces cerevisiae and the microalga Chlorella sorokiniana, identified two specific genes involved in carbon and nitrogen catabolite repression that facilitate mutualistic behaviors between yeast and microalgae when inactive. Taken together the data suggest novel strategies for microbial ecosystem-based decision making in wine making and improved integration of natural microbial biodiversity in the process.

DOI:

Publication date: February 11, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Florian F. Bauer, Cleo Conacher, Jennifer Oosthuizen, Georgia Strydom, Evodia Setati, Rene Naidoo-Blassoples

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch, South Africa

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

MOVING FROM SULFITES TO BIOPROTECTION: WHICH IMPACT ON CHARDONNAY WINE?

Over the last few years, several tools have been developed to reduce the quantity of sulfites used during winemaking, including bioprotection. Although its effectiveness in preventing the development of spoilage microorganisms has been proven, few data are available on the impact of sulfite substitution by bioprotection on the final product. The objective of this study was therefore to characterize Chardonnay wines with the addition of sulfite or bioprotection in the pre-fermentation stage. The effects of both treatments on resulting matrices was evaluated at several scales: analysis of classical oenological parameters, antioxidant capacity, phenolic compounds, non-volatile metabolome and sensory profile.

FACTORS AFFECTING QUERCETIN SOLUBILITY IN SANGIOVESE RED WINE: FIRST RESULTS

Quercetin (Q) is present in grape in form of glycosides and as aglycone. These compounds are extracted from grape skins during winemaking. In wines, following the hydrolysis reactions, the amount of quercetin aglycon can exceed its solubility value. Unfortunately, a threshold solubility concentration for quercetin in wine is not easy to determine because it depends on wine matrix (Gambuti et al., 2020).

PERCEPTUAL INTERACTIONS PHENOMENA INVOLVING VARIOUS VOLATILE COMPOUND FAMILIES LINKED TO SOME FRUITY NOTES IN BORDEAUX RED WINES

Fruity notes play a key role in the consumer’s appreciation of Bordeaux red wines. If literature provides a lot of knowledge about the nature of volatile compounds involved in this fruity expression, the sensory phenomena involving these compounds in mixture still need to be explored. Considering previous sensory works about the impact of esters and some overripening compounds, the goal of this work was to study the implication of perceptual interactions involving red wine odorant compounds of diverse origins and described as potentially affecting fruity aromatic expression.

Beyond liking scores: the importance of the drinking experience to understand our consumers

The presentation will approach the understanding of wine consumers´ perception based on the experiential model suggested by Warell (2008). In this framework, wine consumption gives rise to a
variety of experiences related to the perception, understanding, and judgment of the product. These
multidimensional facets of the drinking experience can be explored by measuring affective, cognitive,
and sensory responses of consumers, which are shown to be stable regardless of the social context.

NEW INSIGHTS INTO VOLATILE SULPHUR COMPOUNDS SCALPING ON MICROAGGLOMERATED WINE CLOSURES

The evolution of wine during bottle ageing has been of great interest to ensure consistent quality over time. While the role of wine closures on the amount of oxygen is well-known [1], closures could also play other roles such as the scalping phenomenon of flavour compounds. Flavour scalping has been described as the sorption of flavour compounds by the packaging material, which could result in losses of flavour intensity. It has been reported in the literature that volatile sulphur compounds (VSC) can be scalped on wine closures depending on the type of closure (traditional and agglomerated cork, screw-cap, synthetic [2]).