terclim by ICS banner
IVES 9 IVES Conference Series 9 FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Abstract

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

Changes in microbial diversity and dynamics, especially mycobiota colonizing grapes, was evaluated at 5 berry ripening and vinification stages in 31 vineyards for two harvests. Grapes were collected at fruit set, veraison and harvest and micro-pressing (40 kg) and micro-vinifications (5L) of grape musts from each vineyard were performed.

For both harvests, fungal counts increased during berry ripening (although lower counts were observed in 2022 versus 2021), remained relatively high in musts before yeasts solely dominated in laboratory wines. Clear shifts in mycobiota diversity were observed from vine to laboratory wine for both years. Berries were dominated by yeasts (including filamentous Aureobasidium spp.) regardless of ripening stage with an increase in mold diversity during ripening. Cladosporium (7 species identified) were predominant in unmature berries before other molds colonized grapes, especially Penicillium (9 species identified) and Botrytis cinerea, from veraison onwards. Metagenetic analyses (equivalent to 450 samples in 2021) were well correlated with culture-dependent data. This approach confirmed the predominance of yeasts (Aureobasidium and Vishniacozyma) at both fruit set and veraison, in addition to Cladosporium, although higher fungal diversity and variability between musts was observed.

This unprecedent and thorough description of mycobiota from unmature berries to microvinified wines will clearly contribute to a better understanding of the fungal determinants of specific traits linked to wine quality or defects. Microbial co-occurrence networks and global analyses with the generated climatic data and vineyard practices is currently being explored to understand species interactions and identify factors shaping mycobiota composition.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Adrien Destanque1,2*, Adeline Picot1, Flora Pensec1, Nolwenn Rolland1, Audrey Pawtowski1, Sylvie Treguer-Fernandez1, Lau-rence Guérin2, Laurence Mercier2, Emmanuel Coton1, Marion Hervé2 and Monika Coton1

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, 51530 Oiry, France

Contact the author*

Keywords

Mycobiota, Diversity, Metagenetics, Co-occurrence networks

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EFFECT OF FERMENTATION TEMPERATURE GRADIENT AND SKIN CONTACT ON ESTER AND THIOL PRODUCTION AND TROPICAL FRUIT PERCEPTION IN CHARDONNAY WINES

Wines with tropical fruit aromas have become increasingly more available1,2. With increased availability of different wine styles, it has become important to understand the compounds that cause the fruity aromas in wine. Previous work using micro fermentations showed that fermentation temperature gradients and time on skins resulted in an increase in thiol and ester compounds post fermentation and these compounds are known to cause tropical fruit aroma in wines³. This work aimed to scale up these fermentations/operations to determine if the desired aromas could still be achieved and if there is a perceivable difference in tropical fruit aromas, liking, and emotional response in the wines at the consumer level.

PHENOLICS DYNAMICS OF BERRIES FROM VITIS VINIFERA CV SYRAH GRAFTED ON TWO CONTRASTING ROOTSTOCKS UNDER COMBINED SALINITY AND WATER STRESSORS AND ITS EFFECT ON WINE QUALITY

Wine regions are getting warmer as average temperatures continue raising affecting grape growth, berry composition and wine production. Berry quality was evaluated in plants of Vitis vinifera cv Syrah grafted on two rootstocks, Paulsen (PL1103) and SO4, and grown under two salinity concentrations (LS:0.7dS/m and HS:2.5dSm-1) in combination with two irrigation regimes (HW:133% and CW:100%), being the seasonal water application 483mm (control, 100%). Spectrophotometer measurements from berry skin during veraison and harvest stages and from “young” wine samples, were indicative of the stressors effect and the mediation of the rootstocks. At veraison (i) total phenolics content were high under LSHW (0.7dSm-1 and high water conditions) for SO4 and PL1103.

PROFILING OF LIPIDS IN WINES FROM MONOCULTURE FERMENTATION WITH INDIGENOUS METSCHNIKOWIA YEAST SPECIES

Lipids are a diverse group of organic compounds essential for living systems. They are vital compounds for yeast which makes them an important modulator of yeast metabolism in alcoholic fermentation. This study presents a comprehensive lipidome analysis of wine samples from the Vitis vinifera L., Maraština. The fermentation trails were set up in monoculture with different indigenous yeast strains selected from a collection of native yeasts established at the Institute for Adriatic Crops and Karst Reclamation in 2021, previously isolated from Croatian Maraština grapes: Metschnikowia pulcherrima, Metshnikowia sinensis/shanxiensis , and Metschnikowia chyrsoperlae.

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: EVOLUTION IN BOTTLED WINE

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors. The impact on wine aroma is mainly attributed to volatile phenols, while in-mouth hydrolysis of glycosylated forms may be responsible for long-lasting “ashy” aftertastes (1).

MOUSY OFF-FLAVOURS IN WINES: UNVEILING THE MICROORGANISMS BEHIND IT

Taints and off-flavours are one of the major concerns in the wine industry and even if the issues provoked by them are harmless, they can still have a negative impact on the quality or on the visual perception of the consumer. Nowadays, the frequency of occurrence of mousy off-flavours in wines has increased.
The reasons behind this could be the significant decrease in sulphur dioxide addition during processing, the increase in pH or even the trend for spontaneous fermentation in wine. This off-flavour is associated with Brettanomyces bruxellensis or some lactic acid bacteria metabolisms.