terclim by ICS banner
IVES 9 IVES Conference Series 9 FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Abstract

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

Changes in microbial diversity and dynamics, especially mycobiota colonizing grapes, was evaluated at 5 berry ripening and vinification stages in 31 vineyards for two harvests. Grapes were collected at fruit set, veraison and harvest and micro-pressing (40 kg) and micro-vinifications (5L) of grape musts from each vineyard were performed.

For both harvests, fungal counts increased during berry ripening (although lower counts were observed in 2022 versus 2021), remained relatively high in musts before yeasts solely dominated in laboratory wines. Clear shifts in mycobiota diversity were observed from vine to laboratory wine for both years. Berries were dominated by yeasts (including filamentous Aureobasidium spp.) regardless of ripening stage with an increase in mold diversity during ripening. Cladosporium (7 species identified) were predominant in unmature berries before other molds colonized grapes, especially Penicillium (9 species identified) and Botrytis cinerea, from veraison onwards. Metagenetic analyses (equivalent to 450 samples in 2021) were well correlated with culture-dependent data. This approach confirmed the predominance of yeasts (Aureobasidium and Vishniacozyma) at both fruit set and veraison, in addition to Cladosporium, although higher fungal diversity and variability between musts was observed.

This unprecedent and thorough description of mycobiota from unmature berries to microvinified wines will clearly contribute to a better understanding of the fungal determinants of specific traits linked to wine quality or defects. Microbial co-occurrence networks and global analyses with the generated climatic data and vineyard practices is currently being explored to understand species interactions and identify factors shaping mycobiota composition.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Adrien Destanque1,2*, Adeline Picot1, Flora Pensec1, Nolwenn Rolland1, Audrey Pawtowski1, Sylvie Treguer-Fernandez1, Lau-rence Guérin2, Laurence Mercier2, Emmanuel Coton1, Marion Hervé2 and Monika Coton1

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, 51530 Oiry, France

Contact the author*

Keywords

Mycobiota, Diversity, Metagenetics, Co-occurrence networks

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

Grape pomace is one of the main by-products generated after pressing in winemaking.Emerging methods, such as ultrasound-assisted extraction with eutectic mixtures, have great potential due to their low toxicity, and high biodegradability. Choline chloride (ChCl) was used as a hydrogen bond acceptor and its corresponding hydrogen bond donor (malic acid, citric acid, and glycerol: urea). Components were heated at 80 °C and stirred until a clear liquid was obtained. Distilled water was added (30 % v/v). A solid-liquid ratio of 1 g pomace per 10 ml of eutectic solvent was used.

FOURIER TRANSFORM INFRARED SPECTROSCOPY IN MONITORING THE WINE PRODUCTION

The complexity of the wine matrix makes the monitoring of the winemaking process crucial. Fourier Transform Infrared Spectroscopy (FTIR) along with chemometrics is considered an effective analytical tool combining good accuracy, robustness, high sample throughput, and “green character”. Portable and non-portable FTIR devices are already used by the wine industry for routine analysis. However, the analytical calibrations need to be enriched, and some others are still waiting to be thoroughly developed.

OPTIMIZATION, VALIDATION AND APPLICATION OF THE EPR SPIN-TRAPPING TECHNIQUE TO THE DETECTION OF FREE RADICALS IN CHARDONNAY WINES

The aging potential of Burgundy chardonnay wines is considered as quality indicator. However, some of them exhibit higher oxidative sensitivity and premature oxidative aging symptoms, which are potentially induced by no-enzymatic oxidation such as Fenton-type reaction (Danilewicz, 2003). This chemical mechanism involves the action of transition metal, native phenolic compounds and oxygen which promote the generation of highly reactive oxygen species (ROS) such as hydroxyl radicals (OH) or 1-hydroxyethyl radicals (1-HER) from oxidation of ethanol. Such mechanism is involved in the radical oxidation occurring during bottle aging. According to Elias et al.,(2009a), the 1-HER is the most abundant radical in forced oxidation treated wines. Consequently, understanding its evolution kinetic in dry white wines is of great importance.

IMPACT OF NEW BIO STIMULANTS ON GRAPE SECONDARY METABOLITES UNDER CLIMATE CHANGE CONDITIONS

In a context of climate change and excessive use of agrochemical products, sustainable approaches for environmental and human health such as the use of bio stimulants in viticulture represent a potential option, against abiotic and biotic threats. Bio stimulants are organic compounds, microbes, or a combination of both, that stimulate plant’s vital processes, allowing high yields and good quality products. In vines, may trigger an innate immune response leading to the synthesis of secondary metabolites, key compounds for the organoleptic properties of grapes and wines.

PERCEPTUAL INTERACTIONS PHENOMENA INVOLVING VARIOUS VOLATILE COMPOUND FAMILIES LINKED TO SOME FRUITY NOTES IN BORDEAUX RED WINES

Fruity notes play a key role in the consumer’s appreciation of Bordeaux red wines. If literature provides a lot of knowledge about the nature of volatile compounds involved in this fruity expression, the sensory phenomena involving these compounds in mixture still need to be explored. Considering previous sensory works about the impact of esters and some overripening compounds, the goal of this work was to study the implication of perceptual interactions involving red wine odorant compounds of diverse origins and described as potentially affecting fruity aromatic expression.