terclim by ICS banner
IVES 9 IVES Conference Series 9 ANTIOXIDANT CAPACITY OF INACTIVATED NON-SACCHAROMYCES YEASTS

ANTIOXIDANT CAPACITY OF INACTIVATED NON-SACCHAROMYCES YEASTS

Abstract

The importance of the non-Saccharomyces yeasts (NSY) in winemaking has been extensively reviewed in the past for their aromatic or bioprotective capacity while, recently their antioxidant/antiradical potential has emerged under winemaking conditions. In the literature the antioxidant potential of NSY was solely explored through their capacity to improve glutathione (GSH) content during alcoholic fermentation [1], while more and more studies pointed out the activity of the non-glutathione soluble fraction released by yeasts [2].

Our study proposed to combine untargeted UHPLC-Q-ToF MS based metabolomic analysis with DPPH antiradical activity [3] to explore the antioxidant capacity of compounds released by inactivated non-Saccharomyces yeast (INSY) in wine like model solution. In our experimental plan, 3 INSY species were compared to one inactivated Saccharomyces cerevisiae yeast (ISY) selected for its high antioxidant capacity [4]. In that way, both the species and the production process were evaluated for their impact on the metabolic fingerprint and the antioxidant capacity. Then, unsupervised analysis has been used to extract ions correlated with the antioxidant capacity of the INSY.

Our results show that, all the INSY can accumulate GSH during the specific production process with yields ranging from +170% to +360% compared to the corresponding classical production process. Among the tested INSYs, one presenting equivalent antioxidant capacity to the control ISY while was 4 times less concentrated in GSH (4.73+/-0.09 mg/g against 20.95+/-0.34 mg/g, respectively). The principal component analysis of the 3511 ions detected by UHPLC-Q-ToF MS clearly grouped INSY by species, independently of the production process. 73 specific ions presenting strong and significant spearman correlation (rho < -0.6, p-value < 0.05) with the DPPH scores, clustered the most antioxidant INSY and the control Saccharomyces in different groups, indicating that the antioxidant capacity of these two products should be driven by different pools of compounds.

These results are very valuable for future research perspectives while they point out that, first, GSH alone is not relevant to explain the antioxidant capacity of INSY soluble fraction and other more reactive compounds must be considered. Second, they support the fact that INSY antioxidant capacity is essentially driven by a specie specific metabolism and opens an avenue for the selection new species with great enological potential.

 

1. R.L. Binati, I. Larini, E. Salvetti, S. Torriani, Glutathione production by non-Saccharomyces yeasts and its impact on winema-king: A review, Food Res. Int. 156 (2022) 111333. https://doi.org/10.1016/j.foodres.2022.111333.
2. F. Bahut, Y. Liu, R. Romanet, C. Coelho, N. Sieczkowski, H. Alexandre, P. Schmitt-Kopplin, M. Nikolantonaki, R.D. Gougeon, Metabolic diversity conveyed by the process leading to glutathione accumulation in inactivated dry yeast: A synthetic media study, Food Res. Int. 123 (2019) 762–770. https://doi.org/10.1016/j.foodres.2019.06.008.
3. F. Bahut, R. Romanet, N. Sieczkowski, P. Schmitt-Kopplin, M. Nikolantonaki, R.D. Gougeon, Antioxidant activity from inac-tivated yeast: Expanding knowledge beyond the glutathione-related oxidative stability of wine, Food Chem. 325 (2020) 126941. https://doi.org/10.1016/j.foodchem.2020.126941.
4. R. Romanet, C. Coelho, Y. Liu, F. Bahut, J. Ballester, M. Nikolantonaki, R.D. Gougeon, The Antioxidant Potential of White Wines Relies on the Chemistry of Sulfur-Containing Compounds: An Optimized DPPH Assay, Molecules. 24 (2019) 1353. https://doi. org/10.3390/molecules24071353.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Florian Bahut1,4, Nathalie Sieczkowski¹, Rémi Schneider², Zhigen Zhang³, Maria Nikolantonaki⁴ and Régis D. Gougeon⁴

1. Lallemand SAS, 19 rue des Briquetiers, BP59, 31702 Blagnac, France
2. Oenobrands, 2196 Boulevard de la Lironde, Monferrier-sur-Lez, France
3. Lallemand Inc., 1620 rue préfontaine, Montréal, Canada
4. Univ. Bourgogne Franche-Comté, Institut Agro Dijon, PAM UMR A 02.102, Institut Universitaire de la Vigne et du Vin – Jules Guyot, F-21000 Dijon, France

Contact the author*

Keywords

Yeast derivatives, Antioxidant, Wine stability, Non-Saccharomyces

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

Oenococcus oeni is the main lactic acid bacteria (LAB) species which conducts the malolactic fermentation (MLF) in wine. During MLF, O. oeni converts malic acid into lactic acid, which modulates wine aroma composition leading to better balanced organoleptic properties. O. oeni is a highly specialized species only detected in environments containing alcohol such as wine, cider or kombucha. Genome analysis of more than 240 strains showed that they form at least 4 main phylogenetic lineages and several sublineages, which are associated with different beverages or types of wines.

ALCOHOLIC FERMENTATION AND COLOR OF ROSÉ WINES: INVESTIGATIONS ON THE MECHANISMS RESPONSIBLE FOR SUCH DIVERSITY

Color is one of the key elements for the marketing of rosé wines due to their packaging in transparent bottles. Their broad color range is due to the presence of pigments belonging to phenolic compounds extracted from grapes or formed during the wine-making process. However, the mechanisms responsible for such diversity are poorly understood. The few investigations performed on rosé wines showed that their phenolic composition is highly variable, close to that of red wines for the darkest rosés but very different for light ones [1]. Moreover, large variations in the extent of color loss taking place during fermentation have been reported but the mechanisms involved and causes of such variability are unknown.

MODELLING THE AGEING POTENTIAL OF SYRAH RED WINES BY ACCELERATED AGEING TESTS: INFLUENCE OF ANTIOXIDANT ASSAYS AND PHENOLIC COMPOSITION

Red wine ageing is an important step in the red wine evolution and impacts its chemical and sensory characteristics through many chemicals and physico-chemical reactions. The kinetics of these evolutions depend on the wine studied and influence the wine ageing potential. Generally, high quality red wines require a longer period of bottle ageing before consumption¹. The ageing potential is an impor-tant parameter for wine quality and is related to the capacity of a wine to undergo oxidation over time². Phenolic compounds which are ones of the main substrates for oxidation can then potentially modulate ageing potential³.

AGEING BOTTLED WINES SUBMERGED IN SEA: DOES IT IMPACT WINE COMPOSITION?

Aging wines is a common practice in oenology, which in recent years has undergone some innovations. Currently, we are witnessing the practice of aging bottled wine in depth, immersed in the sea or in reservoirs, for variable periods of time, but so far, little is known about the impact of aging in depth on the physicochemical properties, of wines.
The objective of this work was to evaluate the impact of this practice on the physicochemical characteristics, in particular to verify changes in the volatile composition of wines bottled and subsequently immersed in depth. A red wine from Cabernet Sauvignon was bottled and a set of bottles were submerged from July to February (2020), another set of bottles were submerged from February to September (2020) and another set was kept in the wine cellar. Bottles from each set were analyzed (in triplicate) in July 2021.

MAPPING OF GAS-PHASE CO₂ IN THE HEADSPACE OF CHAMPAGNE GLASSES BY USING AN INFRARED LASER SENSOR UNDER STATIC TASTING CONDITIONS

From the chemical angle, Champagne wines are complex hydro-alcoholic mixtures supersaturated with dissolved carbon dioxide (CO₂). During the pouring process and throughout the several minutes of tasting, the headspace of a champagne glass is progressively invaded by many chemical species, including gas-phase CO₂ in large majority. CO₂ bubbles nucleated in the glass and collapsing at the champagne surface act indeed as a continuous paternoster lift for aromas throughout champagne or sparkling wine tasting [1]. Nevertheless, inhaling a gas space with a concentration of gaseous CO₂ close to 30% and higher triggers a very unpleasant tingling sensation, the so-called “carbonic bite”, which might completely perturb the perception of the wine’s bouquet.