terclim by ICS banner
IVES 9 IVES Conference Series 9 BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

Abstract

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product. Recent research took the advantage of the availability of commercial non-Saccharomyces yeast to evaluate their bioprotective potential in oenology. From 2017 to 2021, different experiments were conducted using a mixture of two species (Torulaspora delbrueckii and Metschnikowia pulcherrima) as bioprotection applied at 50 mg/L directly on grapes or musts on different varieties and ripening stages. Our data showed that the bioprotection was successfully implanted in the medium, whith a lower colonization for over-ripened harvests. By using 18S metabarcoding analysis in grape must, we showed that fungal communities such as Hanseniaspora, Aspergillus or Botrytis were significantly less abundant when bio- protection was applied instead of SO₂. Furthermore, bioprotection added in the must rapidly consumed dissolved O₂ and had a negative impact on the strict aerobic acetic acid bacteria by limiting the development of these spoilage microorganisms. Experiments carried out in white must showed that bioprotection also limits the oxidation phenomena: the concentrations of glutathione were significantly higher in bioprotected white musts and final wines. Finally, bioprotection used in red winemaking presents a chemical signature, characterized by fatty acid ethyl esters, increasing the perception of fruitiness in young red wines, but to a lesser extend compared to the same yeast strains in mixed fermentation with S. cerevisiae. After bottling, the bioprotected wines were not sensorially different from wines without SO₂ addition but were different from classical sulphited wines. This research confirm the antimicrobial and a partial protection from oxidation by bioprotection in winemaking and its capacity to preserve sensory properties of wines.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Sara Windholtz1,2,Claudia Nioi1,2, Edouard Pelonnier-Magimel1,2, Joana Coulon³, Emmanuel Vinsonneau⁴, Stéphane Becquet⁵, Georgia Lytra1,2, Cécile Thibon1,2, Isabelle Masneuf-Pomarède1,2

1. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2. Bordeaux Sciences Agro, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33170 Gradignan, France
3. Biolaffort, 11 Rue Aristide Bergès, 33270, Floirac, France
4. Institut Français de la Vigne et du Vin, Blanquefort, France
5. Syndicat des Vignerons Bio Nouvelle-Aquitaine, Montagne, France 

Contact the author*

Keywords

Bioprotection, Non-Saccharomyces, SO₂ alternative

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

PHOTO OXIDATION OF LUGANA WINES: INFLUENCE OF YEASTS AND RESIDUAL NITROGEN ON VSCS PROFILE

Lugana wines are made from Turbiana grapes. In recent times, many white and rosé wines are bottled and stored in flint glass bottles because of commercial appeal. However, this practice could worsen the aroma profile of the wine, especially as regards the development of volatile sulfur compounds (VSCs). This study aims to investigate the consequences of exposure to light in flint bottles on VSCs profile of Lugana wines fermented with two different yeasts and with different post-fermentation residual nitrogen.

PAIRING WINE AND STOPPER: AN OLD ISSUE WITH NEW ACHIEVEMENTS

The sensory characteristics of wine are a topic studied by several researchers over time, but it continues to be a current and challenging subject. These characteristics are fundamental for the consumer acceptability, which has increasingly aroused their interest to modulate them in line with current market trends and innovation demands. The wine physical-chemical and sensory properties depend on a wide set of factors: they begin to be designed in the vineyard and are later constructed during the various stages of winemaking. Afterwards, the wine is placed in bottles and stored or commercialized.

THE EFFECT OF BENTONITE FINING ON THE VOLATILE AND NON-VOLATILE PROFILE OF ITALIAN WHITE WINES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines. Macerations were of 7 days, except in the extended macerations that were of 15 days.

HOLISTIC APPROXIMATION OF THE INFLUENCE OF SACCHAROMYCES STRAINS ON WINE AROMA PRECURSORS

Wine varietal aroma is the result of a mixture of compounds formed or liberated from specific grape-aroma precursors. Their liberation/formation from their specific precursors can occur spontaneously by acid catalyzed rearrangements or hydrolysis or by the action of the yeast enzymatic activities. The influence of yeast during fermentation on the production of these volatile compounds has been widely studied however, the effect of this influence during aging is not fully understood. In order to evaluate these processes several indirect strategies have been used to study aroma precursors although they are not useful to understand the chemistry of the process.

EVALUATING WINEMAKING APPLICATIONS OF ULTRAFILTRATION TECHNOLOGY

Ultrafiltration is a process that fractionates mixtures using semipermeable membranes, primarily on the basis of molecular weight. Depending on the nominal molecular weight cut-off (MWCO) specifications of the membrane, smaller molecules pass through the membrane into the ‘permeate’, while larger molecules are retained and concentrated in the ‘retentate’. This study investigated applications of ultrafiltration technology for enhanced wine quality and profitability. The key objective was to establish to what extent ultrafiltration could be used to manage phenolic compounds (associated with astringency or bitterness) and proteins (associated with haze formation) in white wine.