terclim by ICS banner
IVES 9 IVES Conference Series 9 INSIGHTS ON THE ROLE OF GENES ON AROMA FORMATION OF WINES

INSIGHTS ON THE ROLE OF GENES ON AROMA FORMATION OF WINES

Abstract

Yeast secondary metabolism is a complex network of biochemical pathways and the genetic profile of the yeast carrying out the alcoholic fermentation is obviously important in the formation of the metabolites conferring specific odors to wine. The aim of the present research was to investigate the relative expression of genes involved in flavor compound production in eight different Saccharomyces cerevisiae strains.

Two commercial yeast strains Sc1 (S.cerevisiae x S.bayanus) and Sc2 (S.cerevisiae) and six indigenous S. cerevisiae strains (Sc3, Sc4, Sc5, Sc6, Sc7, Sc8) isolated during spontaneous fermentations were inoculated in Assyrtiko and Vidiano grape must. The fermentation kinetics, content of organic acids and gly- cerol production was monitored daily throughout the experiment. Transcript profiling of yeast genes in- volved in aroma formation and volatile composition of the must/wine was performed through real-time RT-PCR and SPE/GC–MS respectively, at four different time points of the fermentations. Specifically, a total of 28 volatile compounds were determined and the relative expression levels of 46 genes coding for acetyl-CoA synthetases, amino acid permeases, transaminases, reductases, decarboxylases, alcohol and aldehyde dehydrogenases, alcohol acetyltransferases, acyltransferases, esterases and glycosidases were recorded.

The relative expression levels of the genes implicated in amino acids, higher alcohols, ethyl esters, and terpenes metabolism, such as EEB1, EHT1, EXG1, ARO8, ARO9, PDC5, PDC6, ADH6, ADH3, AAD14, AAD16, were higher at the first three time points studied, since the most active period of aroma compound accumulation appears to occur in earlier fermentation stages. Additionally, the transcriptome data revealed substantial changes in expression patterns of genes between the different strains tested. In terms of the volatile characterization of the wines, the concentration levels of total esters and total alcohols appeared to be clearly distinct between the wines, which confirms that the production of volatile compounds is strain depended. Remarkable differences in the gene expression levels were observed when comparing the different strains which resulted in different aroma profiles.

This study enhances our understanding on yeast aroma metabolism-related gene expression and regulation. This knowledge can be a tool to modulate aroma production and orient the fermentation process towards a desirable wine aromatic profile.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Despina Lola¹, Chrysanthi Kalloniati², Emmanouil Flemetakis², Yorgos Kotseridis¹

1. Laboratory of Enology and Alcoholic Drinks, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece
2. Laboratory of Molecular Biology, Department of Biotechnology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece

Contact the author*

Keywords

gene expression, Saccharomyces cerevisiae, yeast metabolism, volatile profile

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

DEVELOPMENT OF DISTILLATION SENSORS FOR SPIRIT BEVERAGES PRODUCTION MONITORING BASED ON IMPEDANCE SPECTROSCOPY MEASUREMENT AND PARTIAL LEAST SQUARES REGRESSION (PLS-R)

During spirit beverages production, the distillate is divided in three parts: the head, the heart, and the tail. Acetaldehyde and ethanol are two key markers which allow the correct separation of distillate. Being toxic, the elimination of the head part, which contains high concentration of acetaldehyde, is crucial to guarantee the consumer’s health and security. Plus, the tail should be separated from the heart based on ethanol concentration.

WINE RACKING IN THE WINERY AND THE USE OF INERT GASES: CONTROL AND OPTIMIZATION OF THE PROCESS

Atmospheric oxygen (O₂) generates oxidation in wines that affect their physicochemical and sensory evolution. The O₂ uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O₂ uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The aim was to study the uptake of O₂ during the racking of a model wine as a reference and to compare with purging the destination tank with different inert gases.

OTA DEGRADATION BY BACTERIAL LACCASEST

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degradation, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking.

Grouping Vitis vinifera grapevine varieties based on their aromatic composition

Climate change is likely to impact wine typicity across the globe, raising concerns in wine regions historically renowned for the quality of their terroir1. Amongst several changes in viticultural practices, replacing some of the planting material (i.e. clones, rootstocks and cultivars) is thought to be one of the most promising potential levers to be used for adapting to climate change. But the change of cultivars also involves the issue of protecting the region’s wine typicity. In Bordeaux (France), extensive research has been conducted on identifying meridional varieties that could be good candidates to help guard against the effects of climate change2 while less research has been done concerning their impacts on Bordeaux wine typicity.

PAIRING WINE AND STOPPER: AN OLD ISSUE WITH NEW ACHIEVEMENTS

The sensory characteristics of wine are a topic studied by several researchers over time, but it continues to be a current and challenging subject. These characteristics are fundamental for the consumer acceptability, which has increasingly aroused their interest to modulate them in line with current market trends and innovation demands. The wine physical-chemical and sensory properties depend on a wide set of factors: they begin to be designed in the vineyard and are later constructed during the various stages of winemaking. Afterwards, the wine is placed in bottles and stored or commercialized.