terclim by ICS banner
IVES 9 IVES Conference Series 9 FUNGAL CHITOSAN IS AN EFFICIENT ALTERNATIVE TO SULPHITES IN SPECIFIC WINEMAKING SITUATIONS

FUNGAL CHITOSAN IS AN EFFICIENT ALTERNATIVE TO SULPHITES IN SPECIFIC WINEMAKING SITUATIONS

Abstract

The most common method to prevent or eliminate microbes in wine is sulfur dioxide (SO₂) addition. However, as risk of acute allergy exists, the European Union has classified SO₂ as one of the 14 priority food allergens (EU Regulation N°1169/2011, Annex II). The legal dose admitted in both conventional and organic farming will be downgraded probably in the near future, according to consumer’s expectations. In addition, sulfur dioxide addition does not always prevent microbial spoilage, because of the emergence of tolerant/resistant strains. Winemakers thus need alternate and efficient antiseptic methods to reduce total SO₂ content in wines. The resolutions of the 7th general assembly of the International Organization of Vine and Wine (OIV/OENO 338A/2009) and the European Union (EC 53/2011) authorized the addition of fungal chitosan to reduce spoilage microorganism populations especially Brettanomyces bruxellensis. Chitosan is a partially acetylated polysaccharide of glucosamine. It is positively charged at wine pH, which allows it to interact with the microorganisms and particles present in the wine. With the trend in oenology of limiting SO₂, more and more questions arise as to the impact of fungal chitosan on other microorganisms from grapes and wine-related environment. It was shown recently that most species were affected, at least transiently, by chitosan treatment (Miot-Sertier et al. 2022). However, a high variability prevails within most species and sensitive, intermediate and tolerant strains can be observed, as well as different efficiencies depending on the wine chemical parameters or the winemaking stage when the treatment is performed.

In order to have a clear opinion on the usefulness of a chitosan treatment, we have carried out tests in various situations in which sulphites were not enough to protect the wine (presence of tolerant strains in particular). Though chitosan does not solve all the microbial spoilage issues, this study reveals that chitosane can be an interesting alternative to sulphites in certain situations. Furthermore, when the antiseptic effect is clear it seems durable and hence, wines are protected for microbial spoilage over long periods.
The study also shows that structural differences among fungal chitosans impact their efficiency. The organoleptic consequences of the treatment are also evaluated on red and white wines.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Cécile Miot-Sertier¹, Margot Paulin¹, Axel Marchal¹, Patricia Ballestra¹, Warren Albertin¹, Isabelle Masneuf Pomarède¹, Joana Coulon², Virginie Moine², Amélie Vallet-Courbin³, Julie Maupeu³, Thierry Doco⁴, Cédric Delattre5-6,Marguerite Dols-Lafargue¹

1. Univ. Bordeaux, INRAE, Bordeaux INP, Bordeaux Sciences Agro, OENO, UMR1366, ISVV, F-33140 Villenave d’Ornon, France
2. Biolaffort, 11 rue Aristide Bergès, 33270 Floirac, France
3. Microflora-ADERA, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
4. UMR 1083, UMR Sciences pour l’Oenologie, INRA, SupAgro, UM1, 2 place Viala, F-34060 Cedex Montpellier, France
5. Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France
6. Institut Universitaire de France (IUF), 1 Rue Descartes, 75000 Paris, France

Contact the author*

Keywords

Antiseptic, Spoilage, Chitosan, Sulfites

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Molecular approaches for understanding and modulating wine taste

Wine consumers generally demand wines having a perception of softer tannins and less ripe, having a heaviness and richness on palate (full-body wine) with a limpid and stable color. However, polyphenol (tannins)-rich wines have been also correlated with unpleasant taste properties such as astringency and bitterness when perceived at high intensities. Modulating these unpleasant properties could be important for consumer’s approval of wines.

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products.

THE EFFECT OF DIFFERENT TERROIRS ON AROMA COMPOUNDS OF ‘KALECIK KARASI’ WINES

Kalecik Karası is a domestic grape variety of Turkey, originating from Kalecik district, 80 km from Ankara. Although there is no definite evidence, it is known that it was used in wine production by many civilizations that lived in the Anatolian region, especially the Hittites. Compared to other black wine grapes, it stands out with its low tannin content, rich fruity aroma and complex structure. In good vintages, red fruits such as strawberries, cherries and raspberries stand out in the aroma profile. Although its structure is elegant, it has the potential to age and develop similar to the ‘Pinot Noir’ wine of the Burgundy region. This offers a complex aroma structure including red flowers, earth and ripe fruits.

TANNINS AND ANTHOCYANINS KINETICS OF EXTRACTION FROM ARINARNOA, MARSELAN AND TANNAT UNDER DIFFERENT WINEMAKING TECHNIQUES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines.

SENSORY EVALUATION OF WINE AROMA: SHOULD COLOR-DRIVEN DESCRIPTORS BE USED?

The vocabulary used to describe wine aroma is commonly organized according to color, raising the question of whether they reflect the reality of olfactory perception. Previous studies have assumed this convention of color-aroma matching, and have investigated color’s influence on the perception of aroma only in dyed white wine or in red wine from particular places of origin. Here 48 white and red varietal wines from around the world were evaluated in black glasses then in clear glasses by a panel of wine experts, who gave intensity ratings for aroma attributes commonly used by wine professionals. In black glasses, aromas conventionally associated with white wine were perceived in the red wines, and vice versa.