terclim by ICS banner
IVES 9 IVES Conference Series 9 FUNGAL CHITOSAN IS AN EFFICIENT ALTERNATIVE TO SULPHITES IN SPECIFIC WINEMAKING SITUATIONS

FUNGAL CHITOSAN IS AN EFFICIENT ALTERNATIVE TO SULPHITES IN SPECIFIC WINEMAKING SITUATIONS

Abstract

The most common method to prevent or eliminate microbes in wine is sulfur dioxide (SO₂) addition. However, as risk of acute allergy exists, the European Union has classified SO₂ as one of the 14 priority food allergens (EU Regulation N°1169/2011, Annex II). The legal dose admitted in both conventional and organic farming will be downgraded probably in the near future, according to consumer’s expectations. In addition, sulfur dioxide addition does not always prevent microbial spoilage, because of the emergence of tolerant/resistant strains. Winemakers thus need alternate and efficient antiseptic methods to reduce total SO₂ content in wines. The resolutions of the 7th general assembly of the International Organization of Vine and Wine (OIV/OENO 338A/2009) and the European Union (EC 53/2011) authorized the addition of fungal chitosan to reduce spoilage microorganism populations especially Brettanomyces bruxellensis. Chitosan is a partially acetylated polysaccharide of glucosamine. It is positively charged at wine pH, which allows it to interact with the microorganisms and particles present in the wine. With the trend in oenology of limiting SO₂, more and more questions arise as to the impact of fungal chitosan on other microorganisms from grapes and wine-related environment. It was shown recently that most species were affected, at least transiently, by chitosan treatment (Miot-Sertier et al. 2022). However, a high variability prevails within most species and sensitive, intermediate and tolerant strains can be observed, as well as different efficiencies depending on the wine chemical parameters or the winemaking stage when the treatment is performed.

In order to have a clear opinion on the usefulness of a chitosan treatment, we have carried out tests in various situations in which sulphites were not enough to protect the wine (presence of tolerant strains in particular). Though chitosan does not solve all the microbial spoilage issues, this study reveals that chitosane can be an interesting alternative to sulphites in certain situations. Furthermore, when the antiseptic effect is clear it seems durable and hence, wines are protected for microbial spoilage over long periods.
The study also shows that structural differences among fungal chitosans impact their efficiency. The organoleptic consequences of the treatment are also evaluated on red and white wines.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Cécile Miot-Sertier¹, Margot Paulin¹, Axel Marchal¹, Patricia Ballestra¹, Warren Albertin¹, Isabelle Masneuf Pomarède¹, Joana Coulon², Virginie Moine², Amélie Vallet-Courbin³, Julie Maupeu³, Thierry Doco⁴, Cédric Delattre5-6,Marguerite Dols-Lafargue¹

1. Univ. Bordeaux, INRAE, Bordeaux INP, Bordeaux Sciences Agro, OENO, UMR1366, ISVV, F-33140 Villenave d’Ornon, France
2. Biolaffort, 11 rue Aristide Bergès, 33270 Floirac, France
3. Microflora-ADERA, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
4. UMR 1083, UMR Sciences pour l’Oenologie, INRA, SupAgro, UM1, 2 place Viala, F-34060 Cedex Montpellier, France
5. Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France
6. Institut Universitaire de France (IUF), 1 Rue Descartes, 75000 Paris, France

Contact the author*

Keywords

Antiseptic, Spoilage, Chitosan, Sulfites

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

MAPPING THE CONCENTRATIONS OF GASEOUS ETHANOL IN THE HEADSPACE OF CHAMPAGNE GLASSES THROUGH INFRARED LASER ABSORPTION SPECTROSCOPY

Under standard wine tasting conditions, volatile organic compounds (VOCs) responsible for the wine’s bouquet progressively invade the glass headspace above the wine surface. Most of wines being complex water/ethanol mixtures (with typically 10-15 % ethanol by volume), gaseous ethanol is therefore undoubtedly the most abundant VOC in the glass headspace [1]. Yet, gaseous ethanol is known to have a multimodal influence on wine’s perception [2]. Of particular importance to flavor perception is the effect of ethanol on the release of aroma compounds into the headspace of the beverage [1].

BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product.

INVESTIGATING TERROIR TYPICITY: A COMPREHENSIVE STUDY BASED ON THE AROMATIC AND SENSORIAL PROFILES OF RED WINES FROM CORBIÈRES APPELLATION

Volatile compounds play a significant role on the organoleptic properties defining wines quality. This particular role was exploited in several studies with the aim to differentiate wines from a more or less extensive production area, according to their sensory profile [1], as well as their chemical composition [2,3] (Di Paola-Naranjo et al., 2011; Kustos et al., 2020). Indeed, since aroma compounds development in grapes depends primarily on the environmental conditions of the vines and grapes (soil and climate), it is conceivable that these parameters craft the aromatic signature of the wine produced, in relation to its origin (Van Leeuwen et al., 2020). In this work, a general study on the aromatic and sensorial profile of wines produced in five sub-regions of the Corbières denomination, a renowned red grape varieties viticultural region in South France, was reported.

IN DEPTH CHARACTERIZATION OF OENOLOGICAL CHARACTERISTICS OF TWO LACHANCEA THERMOTOLERANS STARTER STRAINS

Non-Saccharomyces starter cultures became increasingly popular over the years because of their potential to produce more distinctive and unique wines. The major benefit of the use of Lachancea thermotolerans as a fermentation starter is its ability to produce relevant amounts of lactic acid and reduce alcoholic strength, making it valuable for mitigating negative impacts of climate change on grapes and wine quality. Besides, like any other non-Saccharomyces yeast, L. thermotolerans can significantly affect a whole range of other physico-chemical wine parameters.

MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

The recovery of bioactive compounds from grape and wine by-products is currently an important and necessary objective for sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds such as polyphenols, polysaccharides, fatty acids, minerals and seed oil. Polysaccharides contained in the grape cell wall can be rhamnogalacturonans type II (RG-II), polysaccharides rich in arabinose and galactose (PRAG), mannoproteins (MP), homogalacturonans (HG) and non pectic polysaccharides (NPP).