terclim by ICS banner
IVES 9 IVES Conference Series 9 RED WINE AGING WITHOUT SO₂: WHAT IMPACT ON MICROBIAL COMMUNITY?

RED WINE AGING WITHOUT SO₂: WHAT IMPACT ON MICROBIAL COMMUNITY?

Abstract

Nowadays, the use of food preservatives is controversial, SO2 being no exception. Microbial communities have been particularly studied during the prefermentary and fermentation stages in a context of without added SO2. However, microbial risks associated with SO2 reduction or absence, particularly during the wine aging process, have so far been little studied.  The microbiological control of wine aging is a key issue for winemakers wishing to produce wines without added SO2. The aim of the present study is to evaluate the impact of different wine aging strategies according to the addition or not of SO2 on the microbiological population levels and diversity.

In 2021 and 2022, microbial community were monitored on merlot red wines during the wine aging process with different SO2 management and no SO2 addition. An experimental design (30L) was set up in triplicate and samples were collected from vatting to bottling to perform microbial analysis: population levels were monitored by plating on agar selective media for cultivable yeasts, acetic and lactic acid bacteria. From a subset of colonies obtained on solid medium, identifications at species level were made using the MALDI-TOF MS combining with a homemade database created by the laboratory.

In 2021, our results showed that without SO2, significant higher population levels of yeast and bacteria comparing with the sulphiting wines were present during the wine aging process. As expected, the higher species diversity was found at vatting. During the winemaking process, different species of lactic acid bacteria (10), acetic acid bacteria (3) and yeasts (8) were identified. Surprisingly, the effectiveness of SO2 addition at the end of MLF on the lactic acid bacteria showed contrasting results considering initial SO2 addition or not at vatting: population levels were significantly lower when SO2 was added only after malolactic fermentation. Our results regarding the impact of SO2 management during the winemaking process could provide opportunities for winemakers to reduce SO2 levels.  Furthermore, for the first time, microbial communities have been monitored throughout the winemaking process, in a reduced or without added SO2 context.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Sara Windholtz1,2, Julie Maupeu3, Amelie Vallet Courbin3, Marine Lucas3, Valerian Duarte4, Anne Hubert5, Stéphane Becquet5, Emmanuel Vinsonneau4, Isabelle Masneuf Pomarède1,2

  1. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
  2. Bordeaux Sciences Agro, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33170 Gradignan, France
  3. Microflora-ADERA, Univ. Bordeaux, INRAE, Bordeaux INP, Bordeaux Sciences Agro, UMR 1366 OENOLOGIE, ISVV, F33882 Villenave d’Ornon, France
  4. Institut Français de la Vigne et du Vin, Blanquefort, France
  5. Syndicat des Vignerons Bio Nouvelle-Aquitaine, Montagne, France

Contact the author*

Keywords

wine without SO₂, microbial communities, MALDI-TOF MS, malolactic fermentation

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

THE ODORIFEROUS VOLATILE CHEMICALS BEHIND THE OXIDATIVE AROMA DEGRADATION OF SPANISH RED WINES

It is a well-established fact that premature oxidation is noxious for wine aromatic quality and longevity. Although some oxidation-related aroma molecules have been previously identified, there are not works carrying out systematic research about the changes in the profiles of odour-active volatiles during wine oxidation.

REVEALING THE ORIGIN OF BORDEAUX WINES WITH RAW 1D-CHROMATOGRAMS

Understanding the composition of wine and how it is influenced by climate or wine-making practices is a challenging issue. Two approaches are typically used to explore this issue. The first approach uses chemical
fingerprints, which require advanced tools such as high-resolution mass spectrometry and multidimensional chromatography. The second approach is the targeted method, which relies on the widely available 1-D GC/MS, but involves integrating the areas under a few peaks which ends up using only a small fraction of the chromatogram.

IMPACT OF NEW BIO STIMULANTS ON GRAPE SECONDARY METABOLITES UNDER CLIMATE CHANGE CONDITIONS

In a context of climate change and excessive use of agrochemical products, sustainable approaches for environmental and human health such as the use of bio stimulants in viticulture represent a potential option, against abiotic and biotic threats. Bio stimulants are organic compounds, microbes, or a combination of both, that stimulate plant’s vital processes, allowing high yields and good quality products. In vines, may trigger an innate immune response leading to the synthesis of secondary metabolites, key compounds for the organoleptic properties of grapes and wines.

IMPACT OF CLIMATIC CONDITIONS ON THE SEASONING QUALITY OF OAK WOOD FOR OENOLOGICAL USE (QUERCUS PETRAEA)

For coopers, seasoning and toasting are considered crucial steps in barrel making during which the oak wood develops specific organoleptic properties. Seasoning, carried out in the open air, allows reducing the moisture content of the staves to between 14 and 18% (compared to 70 to 90% after splitting) while modulating the intrinsic composition of the oak wood. Toasting consists of applying different degrees of heat to a barrel for a specific period of time. As the temperature increases, oak wood produces a wide range of chemical compounds through thermal degradation of its intrinsic composition.

EFFECTS OF INDUCED SUNBURN DAMAGES ON WHITE WINE PROPERTIES

Climate change is a great challenge for the environment and affects the wine industry as well. Sunburn damage of sensitive grapes increase with severe heat periods. Besides significant loss of yield sunburn, modifies sensory properties of the wines and may cause climate-related off-flavours. To initiate sunburn in a controlled way, in 2021 sunburn was directly induced in the vineyard with the GrapeBurner device, exposing grapes of the varieties Riesling and Pinot Blanc with UV and IR radiation. This device was first assembled by Kai Müller of the university in Geisenheim and consists of a carriage with 6 UV/IR lamps. A 15 min irradiation was applied in early September at 60°Oe. Due to the colder season in 2021 the grapes were not harmed by previous sunburn damage.