OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analytical developments from grape to wine, spirits : omics, chemometrics approaches… 9 D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

Abstract

Studying wine metabolome through multiple targeted methods is complicated and limitative; since grapes, yeasts, bacteria, oxygen, enological techniques and wine aging collaborate to deliver one of the richest metabolomic fingerprint. Therefore, untargeted metabolomics, that developed and evolved as a consequence of the need to obtain a comprehensive characterization of the organic molecules in any biological sample, is the current methodology offering the best coverage of wine metabolome. Taking into account the large genetic diversity, the diversity of the climate and of the agronomical practices, and the wide winemaking culture characterizing the Italian wines, the metabolomic untargeted approach appears as an appropriate analytical tool to study such metabolic space. 

According to the national project D-Wines, 110 single-cultivar red wines from the 2016 vintage were collected directly from wineries across different regions of Italy: Sangiovese from Tuscany and Romagna, Nebbiolo from Piemont, Aglianico from Campania, Nerello Mascalese from Sicily, Primitivo from Apulia, Raboso and Corvina from Veneto, Cannonau from Sardinia, Teroldego from Trentino, Sagrantino from Umbria, and Montepulciano from Abruzzo. The wines were analyzed according to a well-defined RP-UPLC-HRMS-QTOF-MS protocol. 

The results of the data analysis, after their validation: a) confirmed untargeted LC-MS-based metabolomics as a powerful authenticity tool; b) provided indications about the similarity between the cultivars, clustering the wines in three major groups (Primitivo – Nebbiolo, Corvina, Raboso, Sangiovese – Teroldego, Sagrantino, Cannonau, Nerello, Aglianico, Montepulciano); c) furnished a rich list of putative markers characterizing each cultivar, where Primitivo, Teroldego and Nebbiolo had the maximum number of unique putative markers; d) revealed that the putative markers were not only phenolic metabolites; and e) pointed out rt/mz chromatographic sections helpful to distinguish each cultivar from the others. 

This study, together with other D-Wines analytical results, is directed to understand the diversity of Italian red wines and to characterize them in term of metabolic space coverage/variability and taste and in consequence comprehend better their quality. 

Acknowledgements

MIUR project N. 20157RN44Y. A. Curioni, A. Gambuti, V. Gerbi, S. Giacosa, G.P. Parpinello, D. Perenzoni, P. Piombino, A. Rinaldi, S. Río Segade, B. Simonato, G. Tornielli, S. Vincenzi

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Panagiotis Arapitsas, Maurizio Ugliano, Matteo Marangon, Luigi Moio, Luca Rolle, Andrea Versari, Fulvio Mattivi

Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Italy)
Department of Biotechnology, University of Verona (Italy)
Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova (Italy)
Department of Agricultural Sciences, University of Naples Federico II, Avellino (Italy); Dipartimento di Scienze Agrarie, Forestali e Alimentari, Universitàdi Torino (Italy)
Department of Agricultural and Food Sciences, University of Bologna (Italy); Centre Agriculture Food Environment, University of Trento (Italy)

Contact the author

Keywords

mass spectrometry, wine authenticity, bioinformatics, metabolomics 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

First step in the preparation of a soil map of the Protected Designation of Origin Valdepeñas (Central, Spain)

This work is a first step to make a map of vineyard soils. The characterization of the soils of the Protected Designation of Origin (D.P.O.) Valdepeñas will allow to group the studied profiles according to their physico-chemical characteristics and the concentrations of most relevant chemical elements. 90 soil profiles were analysed throughout the territory and the soils were sampled and described according to FAO (2006) and classified according to and Soil Taxonomy (2014). All samples were air dried, sieved and some physico-chemical parameters were determined following standard protocols. Also, major and trace elements were analysed by X-ray fluorescence. The statistically study was made using the SPSS program. Trend maps were made using the ArcGIS program. The studied soils have the following average properties: pH, 8.3; electrical conductivity, 0,20 dS/m (low); clay, 18.8% (medium) and CaCO3, 17.1% (high). In the study for the major elements. The major elements of these soils are Si, followed by Ca and Al, with an average content of 203.7 g/kg, 105.5 g/kg and 74.0 g/kg respectively. On the other hand, 27 trace elements have been studied. Of all of them, it can be highlighted the average values of Ba (361.8 mg/kg), Sr (129.3 mg/kg), Rb (83.4 mg/kg), V (74.2 mg/kg) and Ce (70.6 mg/kg). Ba, V and Ce values are higher and the values of Sr and Rb are lower to those found in the literature. The discriminant analysis shows a percentage of grouping of 91%. The content of chemical elements together with the physico-chemical characteristics allows grouping the soils in 4 group according to their order in the classification to Soil Taxonomy; due to the importance of the Calcisols in Castilla-La Mancha, it has been decided to establish them as their own group even if they do not appear in Soil Taxonomy classification.

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.