OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analytical developments from grape to wine, spirits : omics, chemometrics approaches… 9 D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

Abstract

Studying wine metabolome through multiple targeted methods is complicated and limitative; since grapes, yeasts, bacteria, oxygen, enological techniques and wine aging collaborate to deliver one of the richest metabolomic fingerprint. Therefore, untargeted metabolomics, that developed and evolved as a consequence of the need to obtain a comprehensive characterization of the organic molecules in any biological sample, is the current methodology offering the best coverage of wine metabolome. Taking into account the large genetic diversity, the diversity of the climate and of the agronomical practices, and the wide winemaking culture characterizing the Italian wines, the metabolomic untargeted approach appears as an appropriate analytical tool to study such metabolic space. 

According to the national project D-Wines, 110 single-cultivar red wines from the 2016 vintage were collected directly from wineries across different regions of Italy: Sangiovese from Tuscany and Romagna, Nebbiolo from Piemont, Aglianico from Campania, Nerello Mascalese from Sicily, Primitivo from Apulia, Raboso and Corvina from Veneto, Cannonau from Sardinia, Teroldego from Trentino, Sagrantino from Umbria, and Montepulciano from Abruzzo. The wines were analyzed according to a well-defined RP-UPLC-HRMS-QTOF-MS protocol. 

The results of the data analysis, after their validation: a) confirmed untargeted LC-MS-based metabolomics as a powerful authenticity tool; b) provided indications about the similarity between the cultivars, clustering the wines in three major groups (Primitivo – Nebbiolo, Corvina, Raboso, Sangiovese – Teroldego, Sagrantino, Cannonau, Nerello, Aglianico, Montepulciano); c) furnished a rich list of putative markers characterizing each cultivar, where Primitivo, Teroldego and Nebbiolo had the maximum number of unique putative markers; d) revealed that the putative markers were not only phenolic metabolites; and e) pointed out rt/mz chromatographic sections helpful to distinguish each cultivar from the others. 

This study, together with other D-Wines analytical results, is directed to understand the diversity of Italian red wines and to characterize them in term of metabolic space coverage/variability and taste and in consequence comprehend better their quality. 

Acknowledgements

MIUR project N. 20157RN44Y. A. Curioni, A. Gambuti, V. Gerbi, S. Giacosa, G.P. Parpinello, D. Perenzoni, P. Piombino, A. Rinaldi, S. Río Segade, B. Simonato, G. Tornielli, S. Vincenzi

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Panagiotis Arapitsas, Maurizio Ugliano, Matteo Marangon, Luigi Moio, Luca Rolle, Andrea Versari, Fulvio Mattivi

Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Italy)
Department of Biotechnology, University of Verona (Italy)
Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova (Italy)
Department of Agricultural Sciences, University of Naples Federico II, Avellino (Italy); Dipartimento di Scienze Agrarie, Forestali e Alimentari, Universitàdi Torino (Italy)
Department of Agricultural and Food Sciences, University of Bologna (Italy); Centre Agriculture Food Environment, University of Trento (Italy)

Contact the author

Keywords

mass spectrometry, wine authenticity, bioinformatics, metabolomics 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Anthocyanin profile is differentially affected by high temperature, elevated CO2 and water deficit in Tempranillo (Vitis vinifera L.) clones

Anthocyanin potential of grape berries is an important quality factor in wine production. Anthocyanin concentration and profile differ among varieties but it also depends on the environmental conditions, which are expected to be greatly modified by climate change in the future. These modifications may significantly modify the biochemical composition of berries at harvest, and thus wine typicity. Among the diverse approaches proposed to reduce the potential negative effects that climate change may have on grape quality, genetic diversity among clones can represent a source of potential candidates to select better adapted plant material for future climatic conditions. The effects of individual and combined factors associated to climate change (increase of temperature, rise of air CO2 concentration and water deficit) on the anthocyanin profile of different clones of Tempranillo that differ in the length of their reproductive cycle were studied. The aim was to highlight those clones more adapted to maintain specific Tempranillo typicity in the future. Fruit-bearing cuttings were grown in controlled conditions under two temperatures (ambient temperature versus ambient temperature + 4ºC), two CO2 levels (400 ppm versus 700 ppm) and two water regimes (well-watered versus water deficit), both in combination or independently, in order to simulate future climate change scenarios. Elevated temperature increased anthocyanin acylation, whereas elevated CO2 and water deficit favoured the accumulation of malvidin derivatives, as well as the acylation and tri-hydroxylation level of anthocyanins. Although the changes in anthocyanin profile observed followed a common pattern among clones, such impact of environmental conditions was especially noticeable in one of the most widely distributed Tempranillo clones, the accession RJ43.

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.

Leaf vine content in nutrients and trace elements in La Mancha (Spain) soils: influence of the rootstock

The use of rootstock of American origin has been the classic method of fighting against Phylloxera for more than 100 years. For this reason, it is interesting to establish if different rootstock modifies nutrient composition as well as trace elements content that could be important for determining the traceability of the vine products. A survey of four classic rootstocks (110-Richter, SO4, FERCAL and 1103-Paulsen) and four new ones (M1, M2, M3 and M4) provided by Agromillora Iberia. S.L.U., all of them grafted with the Tempranillo variety, has been carried out during 2019. The eight rootstocks were planted in pots of 500 cc, on three soils with very different characteristics from Castilla-La Mancha (Spain). In the month of July, the leaves were collected and dried in a forced air oven for seven days at 40ºC. Then, the samples were prepared for the analysis determination, carried out by X-Ray fluorescence spectrometry. The results obtained showed that in the case of content in mineral elements in leaf, separated by soil type, we can report the importance of few elements such as Si, Fe, Pb and, especially, Sr. The rootstock does not influence the composition of the vine leaf for the studied elements that are the most important in determining the geochemical footprint of the soil. The influence of the soil can be discriminated according to some elements such as Fe, Pb, Si and, especially, Sr.