OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analytical developments from grape to wine, spirits : omics, chemometrics approaches… 9 D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

D-wines: use of LC-MS metabolomic space to discriminate italian mono-varietal red wines

Abstract

Studying wine metabolome through multiple targeted methods is complicated and limitative; since grapes, yeasts, bacteria, oxygen, enological techniques and wine aging collaborate to deliver one of the richest metabolomic fingerprint. Therefore, untargeted metabolomics, that developed and evolved as a consequence of the need to obtain a comprehensive characterization of the organic molecules in any biological sample, is the current methodology offering the best coverage of wine metabolome. Taking into account the large genetic diversity, the diversity of the climate and of the agronomical practices, and the wide winemaking culture characterizing the Italian wines, the metabolomic untargeted approach appears as an appropriate analytical tool to study such metabolic space. 

According to the national project D-Wines, 110 single-cultivar red wines from the 2016 vintage were collected directly from wineries across different regions of Italy: Sangiovese from Tuscany and Romagna, Nebbiolo from Piemont, Aglianico from Campania, Nerello Mascalese from Sicily, Primitivo from Apulia, Raboso and Corvina from Veneto, Cannonau from Sardinia, Teroldego from Trentino, Sagrantino from Umbria, and Montepulciano from Abruzzo. The wines were analyzed according to a well-defined RP-UPLC-HRMS-QTOF-MS protocol. 

The results of the data analysis, after their validation: a) confirmed untargeted LC-MS-based metabolomics as a powerful authenticity tool; b) provided indications about the similarity between the cultivars, clustering the wines in three major groups (Primitivo – Nebbiolo, Corvina, Raboso, Sangiovese – Teroldego, Sagrantino, Cannonau, Nerello, Aglianico, Montepulciano); c) furnished a rich list of putative markers characterizing each cultivar, where Primitivo, Teroldego and Nebbiolo had the maximum number of unique putative markers; d) revealed that the putative markers were not only phenolic metabolites; and e) pointed out rt/mz chromatographic sections helpful to distinguish each cultivar from the others. 

This study, together with other D-Wines analytical results, is directed to understand the diversity of Italian red wines and to characterize them in term of metabolic space coverage/variability and taste and in consequence comprehend better their quality. 

Acknowledgements

MIUR project N. 20157RN44Y. A. Curioni, A. Gambuti, V. Gerbi, S. Giacosa, G.P. Parpinello, D. Perenzoni, P. Piombino, A. Rinaldi, S. Río Segade, B. Simonato, G. Tornielli, S. Vincenzi

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Panagiotis Arapitsas, Maurizio Ugliano, Matteo Marangon, Luigi Moio, Luca Rolle, Andrea Versari, Fulvio Mattivi

Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Italy)
Department of Biotechnology, University of Verona (Italy)
Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova (Italy)
Department of Agricultural Sciences, University of Naples Federico II, Avellino (Italy); Dipartimento di Scienze Agrarie, Forestali e Alimentari, Universitàdi Torino (Italy)
Department of Agricultural and Food Sciences, University of Bologna (Italy); Centre Agriculture Food Environment, University of Trento (Italy)

Contact the author

Keywords

mass spectrometry, wine authenticity, bioinformatics, metabolomics 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Grape ripening delaying with combined use of leaf removal and natural shading in Manto negro (Vitis vinifera L.) under deficit irrigation

The increasing frequency of heat waves during grape ripening presents challenges for the production of high-quality wine grapes. This underscores the significance of developing effective irrigation and canopy management techniques to optimize both yield and grape quality.
A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality. In a four-block experimental vineyard at Bodega Ribas in Mallorca, two irrigation treatments—moderate and severe deficit irrigation—were implemented. Within each irrigation plot, three light exposure treatments were randomly assigned, encompassing exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening.

Development of a GRASS-GIS application for the characterization of vineyards in the province of Trento

The physical factors that influence the grape ripening include elevation, slope, aspect, potential global radiation, sun hours and soil type of the vineyards.

Long-term drought resilience of traditional red grapevine varieties from a semi-arid region

In recent decades, the scarcity of water resources in agriculture in certain areas has been aggravated by climate change, which has caused an increase in temperatures, changes in rainfall patterns, as well as an increase in the frequency of extreme phenomena such as droughts and heat waves. Although the vine is considered a drought-tolerant specie, it has to satisfy important water requirements to complete its cycle, which coincides with the hottest and driest months. Achieving sustainable viticulture in this scenario requires high levels of efficiency in the use of water, a scarce resource whose use is expected to be severely restricted in the near future. In this regard, the use of drought-tolerant varieties that are able to maintain grape yield and quality could be an effective strategy to face this change. During three consecutive seasons (2018-2020) the behavior in rainfed regime of 13 traditional red grapevine varieties of the Spain central region was studied. These varieties were cultivated in a collection at Centro de Investigación de la Vid y el Vino de Castilla-La Mancha (IVICAM-IRIAF) located in Tomelloso (Castilla-La Mancha, Spain). Yield components (yield, mean bunch and berry weight, pruning weight), physicochemical parameters of the musts (brix degree, total acidity, pH) and some physiological parameters related with water stress during ripening period (δ13C, δ18O) were analysed. The application of different statistical techniques to the results showed the existence of significant differences between varieties in their response to stressful conditions. A few varieties highlighted for their high ability to adapt to drought, being able to maintain high yields due to their efficiency in the use of water. In addition, it was possible quantify to what extent climate can be a determinant in the δ18O of musts under severe water stress conditions.

The role of œnology in the enhancement of terroir expression

The reality of terroir is reflected by the typicality that it confers on the wine. The relationship between the origin of wine and its quality did already exist before the appearance of œnological science. Producers and merchants have always tried to improve wine quality in order to satisfy their clients.

Cultivation of grapes Chardonnay in soils with management practices biodynamic and conventional

The cultivation of grapes, can be accomplished with the use of different systems and practices of agricultural management, the choice of the system to be followed in the vineyard, depends on the conditions of available resources, these being: natural, economic, social, cultural and territorial. As well, it is relevant to know the characteristics of the soil of the vineyard. In the last decade, has been recurrent use of agricultural practices which date back to milinares traditions, with the aim of promoting a recovery of soil and lead the management of cultivation with less damage to the ecosystem. The study here, aimed to quantify the environmental impacts caused in the use of nutrients in conventional tillage and of grapes in the biodynamic agricultural properties in the state of Rio Grande do Sul- Brazil.