OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Analytical developments from grape to wine, spirits : omics, chemometrics approaches… 9 Q-NMR measurements: quantitative analysis of wine composition applied to Bordeaux red wines authenticity control

Q-NMR measurements: quantitative analysis of wine composition applied to Bordeaux red wines authenticity control

Abstract

Traceability of wine is today a consumer demand and a scientific challenge. The methods of analysis must be able to control three fundamental parameters: the geographical origin, the grape varieties, and the vintage. With these focus, the CIVB supports the creation of a VRAI platform (Wine-Research-Authenticity-Identity) within the ISVV (Institute of Vine and Wine Sciences). This platform aims to develop analytical tools to guarantee the origin of a wine. Quantitative Nuclear Magnetic Resonance (qNMR) may be a great tool to help authenticate wines. The acquisition of a large number of wine parameters requires a small volume (a few hundred microliters) and the analysis is performed in a few minutes. This innovative analytical technique can therefore be useful to characterize wines quality and authenticity particularly in the context of priceless wine. 

A NMR-based metabolomics method was developed to semiautomatically quantify many wine components [1]. An original approach based on similarity score (s-score) was developed for wine comparison. Using this approach, a comparative evaluation of the results obtained for three sets of authentic high-valued wines and suspect wines was studied with two methodologies: (i) usual wine analysis, based on the use of multiple techniques, which is the traditional way of analysis for wine authentication and (ii) q-NMR profiling [2]. In order to consider a global aging uncertainty, samples from the same batch from old vintages were analyzed to estimate aging impact on wine composition. Results showed that q-NMR can detect cases of fraud by comparison with the original wine provided by the estate, according to conclusions of official methods. 

More, a database of commercial French wines was built with q-NMR data to examine the specific Bordeaux red wines fingerprinting. Several statistical analyses were performed to classify wines according to their geographical origin, vintage. Results revealed a singular imprint of Bordeaux wines in comparison with other French wines, with classification rates ranging from 71 % to 100 %. These analysies highlighted several specific metabolites of Bordeaux red wines and showed the influence of terroir in the discrimination. Also, Bordeaux subdivisions were investigated, and effects of wines evolution during bottle aging and vintage were pointed out. These studies provide a global and practical description of the potential of q-NMR for wine authentication. 

[1] Gougeon, L., Da Costa, G., Le Mao, I., Ma, W., Teissedre, P. L., Guyon, F., & Richard, T. (2018). Wine Analysis and Authenticity Using 1H-NMR Metabolomics Data: Application to Chinese Wines. Food Analytical Methods, 11(12), 3425-3434. 
[2] Gougeon, L., Da Costa, G., Richard, T., & Guyon, F. (2019). Wine Authenticity by Quantitative 1H NMR Versus Multitechnique Analysis: a Case Study. Food Analytical Methods, doi: 10.1007/s12161-12018-01425-z.

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Tristan Richard, Louis Gougeon, Grégory Da Costa, François Guyon

1.Université de Bordeaux, OEnologie EA 4577, USC 1366 INRA, INP, Molécules d’Intérêt Biologique (Gesvab), ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France
2.Service Commun des Laboratoires, 3 avenue du Dr. Albert Schweitzer, 33600 Pessac, France

Contact the author

Keywords

wine, authenticity, qNMR, multivariate statistics 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.