Molecular characterization of wines nucleophilic potential by ultra-performance liquid chromatography high resolution mass spectrometry

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance.

The identification and management of intra-vineyard variability are key to precision viticulture, and sensors have been proven to be highly efficient tools for detecting these variations.

Vine growers face an increasing number of decisions, both tactical and strategic, in a context where available data and constraints are on the rise, such as resources, societal, environmental, climatic, and economic factors. This has led to a growing supply of decision support systems (DSS) and softwares to manage vineyards. Facing this new complexity, growers must now consider several options: giving up the use of DSS, using systems that are compatible with each other but may limit their options, or using a single system that may be too complex to use effectively. In this context, itk has expanded its Vintel® tool, which was originally designed for grapevine water status management (irrigation, inter-row, cover-crop, etc.), to include fertilization and disease management.

The analysis of polyfunctional thiols in wine is challenging due to their low abundance and instability within a complex matrix. However, volatile thiols are highly aroma-active, making their accurate quantification in wine at low concentrations crucial [1].

«L’Observatoire Grenache» est un réseau de parcelles qui a été mis en place par l’Institut Rhodanien en Vallée du Rhône sur les millésimes de 1995 à 1999. Composé de 24 parcelles de Vitis vinifera L. cv Grenache noir, ce réseau vise à étudier l’influence du terroir (sol, climat et vigneron) sur la qualité des vins. Les parcelles ont été choisies afin de représenter différentes situations géographiques et géopédologiques de la vallée du Rhône. Le matériel végétal (clone, porte-greffe), la taille (cordon de Royat), la densité et l’âge de la parcelle ont été encadrées. Ainsi les conditions de milieu (sol, climat) et les pratiques du vigneron étaient les principales sources de variations.