terclim by ICS banner
IVES 9 IVES Conference Series 9 NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Abstract

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products. This method aims to provide an accurate approximation of condensed tannin levels, similar to the widely used precipitation assay that involves the polysaccharide polymer methyl cellulose. The new tannin approximation is based on the strong correlation observed between Total Polyphenol Index (TPI) and methyl cellulose precipitable (MCP) tannin assay as both are determined at 280 nm, and using the epicatechin calibration is possible to obtain a value that is equivalent to condensed tannins determined by MCP tannin assay. Thus far, the results have shown a strong correlation between this new method and MCP tannin assay, with an r2 value of 0.83 and a sample size of 60. The present study has included wines from diverse geographical locations and varying ages. In addition to quantifying condensed tannins, the study also aimed to explore potential correlations that may explain differences found in the wines by analysing other polyphenolic parameters. These parameters included catechins determined via the DMACA reaction, anthocyanins based on their structural transformation arising from a change in pH, total polyphenols measured using the Folin-Ciocalteu reaction, TPI, and the chromatic features of wines at 420, 520 and 620 nm. Moreover, the study incorporated analyses of pH, total acidity, and ethanol content to obtain a comprehensive understanding of the wines’ chemical composition. Noteworthy, the study is expanding the sampling to consider more matrices within the winemaking process.

All of the aforementioned parameters, including the condensed tannin, were determined automatically using the BioSystems SPICA® analyzer. The technical simplicity of automated methods for phenolic evaluation, will lead to enhanced efficiency, robustness, and accuracy. Furthermore, these automated methods may facilitate greater field applications, leading to increased profitability and an opportunity to improve wine quality.

 

1. Vignault A, González-Centeno MR, Pascual O, Gombau J, Jourdes M, Moine V, et al. Chemical characterization, antioxidant properties and oxygen consumption rate of 36 commercial oenological tannins in a model wine solution. Food Chem. 2018 Dec 1;268:210–9.
2. Gutiérrez-Escobar R, Aliaño-González MJ, Cantos-Villar E. Wine polyphenol content and its influence on wine quality and properties: A review. Vol. 26, Molecules. MDPI AG; 2021.
3. Ribereau-Gayon P, Glories Y. Handbook of Enology:The Chemistry of Wine Stabilization and Treatments. 2006.
4. Habertson J, Spayd S. Measuring Phenolics in the winery. Am J Enol Vitic. 2006;57(3).

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Marilyn M. García-Tenesaca¹, Andreu Tobeña Montanuy²

1. Biosystems S.A , Costa Brava 30, Barcelona, Spain

Contact the author*

Keywords

Tannins, Polyphenolic profile, Automated methods, Wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

S. CEREVISIAE AND O. ŒNI BIOFILMS FOR CONTINUOUS ALCOHOLIC AND MALOLACTIC FERMENTATIONS IN WINEMAKING

Biofilms are sessile microbial communities whose lifestyle confers specific properties. They can be defined as a structured community of bacterial cells enclosed in a self-produced polymeric matrix and adherent to a surface and considered as a method of immobilisation. Immobilised microorganisms offer many advantages for industrial processes in the production of alcoholic beverages and specially increasing cell densities for a better management of fermentation rates.

NEW PLANT BIOPOLYMERS FOR THE COLLOIDAL STABILITY OF THE COLORING MATTER OF RED WINES

The color as well as the “clarity” of red wines are ones of the qualities required by the consumers. Red wines must have colloidal stability from its bottling to its consumption. The supplementation of red wines with additives, and especially Acacia senegal gum, contributes to its organoleptic properties such as the colloidal stabilization of the coloring matter. In a global perspective of limitation of additives in the field of enology, one of the objectives is notably (i) to reduce the use of additives in wines, by their number and/or their quantity, and (ii) to favor the use of natural additives while preserving the organoleptic and sensory qualities of wines.

OPTIMISATION OF THE AROMATIC PROFILE OF UGNI BLANC WINE DISTILLATE THROUGH THE CONTROL OF ALCOHOLIC FERMENTATION

The online monitoring of fermentative aromas provides a better understanding of the effect of temperature on the synthesis and the loss of these molecules. During fermentation, gas and liquid phase concentrations as well as losses and total productions of volatile compounds can be followed with an unprecedented acquisition frequency of about one measurement per hour. Access to instantaneous production rates and total production balances for the various volatile compounds makes it possible to distinguish the impact of temperature on yeast production (biological effect) from the loss of aromatic molecules due to a physical effect³.

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

BORDEAUX RED WINES WITHOUT ADDED SULFITES SPECIFICITIES: COMPOSITIONAL AND SENSORY APPROACHES TOWARDS HIGHLIGHTING AND EXPLAI-NING THEIR SPECIFIC FRUITINESS AND COOLNESS

With the development of naturality expectations, wines produced without any addition of sulfur dioxide (SO₂) become very popular for consumers and such wines are increasingly present on the market. Recent studies also showed that Bordeaux red wines without added SO₂ could be differentiated from a sensory point of view from similar wines produced with SO₂¹. Thus, the aim of the current study was to characterize from a sensory point of view, specific aromas of wines without added SO₂ and to identify compounds involved.