terclim by ICS banner
IVES 9 IVES Conference Series 9 NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Abstract

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products. This method aims to provide an accurate approximation of condensed tannin levels, similar to the widely used precipitation assay that involves the polysaccharide polymer methyl cellulose. The new tannin approximation is based on the strong correlation observed between Total Polyphenol Index (TPI) and methyl cellulose precipitable (MCP) tannin assay as both are determined at 280 nm, and using the epicatechin calibration is possible to obtain a value that is equivalent to condensed tannins determined by MCP tannin assay. Thus far, the results have shown a strong correlation between this new method and MCP tannin assay, with an r2 value of 0.83 and a sample size of 60. The present study has included wines from diverse geographical locations and varying ages. In addition to quantifying condensed tannins, the study also aimed to explore potential correlations that may explain differences found in the wines by analysing other polyphenolic parameters. These parameters included catechins determined via the DMACA reaction, anthocyanins based on their structural transformation arising from a change in pH, total polyphenols measured using the Folin-Ciocalteu reaction, TPI, and the chromatic features of wines at 420, 520 and 620 nm. Moreover, the study incorporated analyses of pH, total acidity, and ethanol content to obtain a comprehensive understanding of the wines’ chemical composition. Noteworthy, the study is expanding the sampling to consider more matrices within the winemaking process.

All of the aforementioned parameters, including the condensed tannin, were determined automatically using the BioSystems SPICA® analyzer. The technical simplicity of automated methods for phenolic evaluation, will lead to enhanced efficiency, robustness, and accuracy. Furthermore, these automated methods may facilitate greater field applications, leading to increased profitability and an opportunity to improve wine quality.

 

1. Vignault A, González-Centeno MR, Pascual O, Gombau J, Jourdes M, Moine V, et al. Chemical characterization, antioxidant properties and oxygen consumption rate of 36 commercial oenological tannins in a model wine solution. Food Chem. 2018 Dec 1;268:210–9.
2. Gutiérrez-Escobar R, Aliaño-González MJ, Cantos-Villar E. Wine polyphenol content and its influence on wine quality and properties: A review. Vol. 26, Molecules. MDPI AG; 2021.
3. Ribereau-Gayon P, Glories Y. Handbook of Enology:The Chemistry of Wine Stabilization and Treatments. 2006.
4. Habertson J, Spayd S. Measuring Phenolics in the winery. Am J Enol Vitic. 2006;57(3).

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Marilyn M. García-Tenesaca¹, Andreu Tobeña Montanuy²

1. Biosystems S.A , Costa Brava 30, Barcelona, Spain

Contact the author*

Keywords

Tannins, Polyphenolic profile, Automated methods, Wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

ACIDIC AND DEMALIC SACCHAROMYCES CEREVISIAE STRAINS FOR MANAGING PROBLEMS OF ACIDITY DURING THE ALCOHOLIC FERMENTATION

In a recent study several genes controlling the acidification properties of the wine yeast Saccharomyces cerevisiae have been identified by a QTL approach [1]. Many of these genes showed allelic variations that affect the metabolism of malic acid and the pH homeostasis during the alcoholic fermentation. Such alleles have been used for driving genetic selection of new S. cerevisiae starters that may conversely acidify or deacidify the wine by producing or consuming large amount of malic acid [2]. This particular feature drastically modulates the final pH of wine with difference of 0.5 units between the two groups.

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

INVESTIGATING TERROIR TYPICITY: A COMPREHENSIVE STUDY BASED ON THE AROMATIC AND SENSORIAL PROFILES OF RED WINES FROM CORBIÈRES APPELLATION

Volatile compounds play a significant role on the organoleptic properties defining wines quality. This particular role was exploited in several studies with the aim to differentiate wines from a more or less extensive production area, according to their sensory profile [1], as well as their chemical composition [2,3] (Di Paola-Naranjo et al., 2011; Kustos et al., 2020). Indeed, since aroma compounds development in grapes depends primarily on the environmental conditions of the vines and grapes (soil and climate), it is conceivable that these parameters craft the aromatic signature of the wine produced, in relation to its origin (Van Leeuwen et al., 2020). In this work, a general study on the aromatic and sensorial profile of wines produced in five sub-regions of the Corbières denomination, a renowned red grape varieties viticultural region in South France, was reported.

THE ODORIFEROUS VOLATILE CHEMICALS BEHIND THE OXIDATIVE AROMA DEGRADATION OF SPANISH RED WINES

It is a well-established fact that premature oxidation is noxious for wine aromatic quality and longevity. Although some oxidation-related aroma molecules have been previously identified, there are not works carrying out systematic research about the changes in the profiles of odour-active volatiles during wine oxidation.

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.