terclim by ICS banner
IVES 9 IVES Conference Series 9 NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Abstract

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products. This method aims to provide an accurate approximation of condensed tannin levels, similar to the widely used precipitation assay that involves the polysaccharide polymer methyl cellulose. The new tannin approximation is based on the strong correlation observed between Total Polyphenol Index (TPI) and methyl cellulose precipitable (MCP) tannin assay as both are determined at 280 nm, and using the epicatechin calibration is possible to obtain a value that is equivalent to condensed tannins determined by MCP tannin assay. Thus far, the results have shown a strong correlation between this new method and MCP tannin assay, with an r2 value of 0.83 and a sample size of 60. The present study has included wines from diverse geographical locations and varying ages. In addition to quantifying condensed tannins, the study also aimed to explore potential correlations that may explain differences found in the wines by analysing other polyphenolic parameters. These parameters included catechins determined via the DMACA reaction, anthocyanins based on their structural transformation arising from a change in pH, total polyphenols measured using the Folin-Ciocalteu reaction, TPI, and the chromatic features of wines at 420, 520 and 620 nm. Moreover, the study incorporated analyses of pH, total acidity, and ethanol content to obtain a comprehensive understanding of the wines’ chemical composition. Noteworthy, the study is expanding the sampling to consider more matrices within the winemaking process.

All of the aforementioned parameters, including the condensed tannin, were determined automatically using the BioSystems SPICA® analyzer. The technical simplicity of automated methods for phenolic evaluation, will lead to enhanced efficiency, robustness, and accuracy. Furthermore, these automated methods may facilitate greater field applications, leading to increased profitability and an opportunity to improve wine quality.

 

1. Vignault A, González-Centeno MR, Pascual O, Gombau J, Jourdes M, Moine V, et al. Chemical characterization, antioxidant properties and oxygen consumption rate of 36 commercial oenological tannins in a model wine solution. Food Chem. 2018 Dec 1;268:210–9.
2. Gutiérrez-Escobar R, Aliaño-González MJ, Cantos-Villar E. Wine polyphenol content and its influence on wine quality and properties: A review. Vol. 26, Molecules. MDPI AG; 2021.
3. Ribereau-Gayon P, Glories Y. Handbook of Enology:The Chemistry of Wine Stabilization and Treatments. 2006.
4. Habertson J, Spayd S. Measuring Phenolics in the winery. Am J Enol Vitic. 2006;57(3).

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Marilyn M. García-Tenesaca¹, Andreu Tobeña Montanuy²

1. Biosystems S.A , Costa Brava 30, Barcelona, Spain

Contact the author*

Keywords

Tannins, Polyphenolic profile, Automated methods, Wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

A NEW TOOL TO QUANTIFY COMPOUNDS POTENTIALLY INVOLVED IN THE FRUITY AROMA OF RED WINES. DEVELOPMENT AND APPLICATION TO THE STU-DY OF THE FRUITY CHARACTER OF RED WINES MADE FROM VARIOUS GRAPE VARIETIES

A wide range of olfactory descriptors ranging from fresh and jammy fruit notes to cooked and oxidized fruit notes could describe the fruity aroma of red wines [1]. The fruity character of a wine is mainly related to the grape variety selected, to the terroir and the vinification process applied for its conception. In white wines, some volatile compounds confer directly their aroma to the wine while the question of “key” compound is more complex in red wines. According to many studies performed over the past decades, some fruity ethyl esters are directly involved in the fruity perception of red wines while others, present at subthreshold concentrations, participate indirectly to the fruity expression via perceptive interactions [2].

USING CHECK-ALL-THAT-APPLY (CATA) TO CATEGORIZE WINES: A DECISION-MAKING TOOL FOR WINE SELECTION

Bordeaux is the largest appellation vineyard in France. This contrasting vineyard with varied terroirs offers all styles of wine, resulting from the blending of several grape varieties. If these different profiles make the renown of Bordeaux wines, it can appear as a constraint when the aim is to study Bordeaux wines in their diversity. The selection of a representative sample can be performed by a sensory analysis carried out by trained panelists or by wine professionals, which can take several forms: consensus among experts, conventional descriptive analysis, typicality or quality evaluation. However, because of time, economic, and logistical constraints, these methods have limited applications. As an alternative to classical descriptive analysis, more intuitive methods that do not require training have been proposed recently to describe wines using an expert panel such as Napping, Free Choice or Flash Profiling, CATA or RATA.

EFFECT OF DIFFERENT VITICULTURAL AND ENOLOGICAL PRACTICES ON THE PHENOLIC COMPOSITION OF RED WINES

Global climate change is exerting a notable influence on viticulture sector and grape composition. The increase in temperature and the changes in rainfall pattern are causing a gap between phenolic and technological grape maturities [1]. As a result, the composition of grapes at harvest time and, consequently, that of wines are being affected, especially with regards to phenolic composition. Hence, wine quality is decreasing due to changes in the organoleptic properties, such as color and astringency, making necessary to implement new adaptive technologies in wineries to modulate these properties in order to improve wine quality.

ANTI-TRANSPIRANT MODULATION OF GRAPE RIPENING: EFFECTS ON MERLOT VINE DEVELOPMENT AND ROSÉ WINE PHENOLIC AND AROMATIC PROFILES

Climate changes are impacting viticultural regions throughout the world with temperature increases being most prevalent.1 These changes will not only impact the regions capable of growing grapes, but also
the grapes that can be grown.2 As temperatures rise the growing degree days increase and with it the sugar accumulation within the berries and subsequent alcohol levels in wine. Consequently, viticultural
practices need to be examined to decrease the levels of sugars.

ASSESSMENT OF GRAPE QUALITY THROUGH THE MONITORING OFPHENOLIC RIPENESS AND THE APPLICATION OF A NEW RAPID METHOD BASED ON RAMAN SPECTROSCOPY

The chemical composition of grape berries at harvest is one of the key aspects influencing wine quality and depends mainly on the ripeness level of grapes. Climate change affects this trait, unbalancing technological and phenolic ripeness, and this further raises the need for a fast determination of the grape maturity in order to quickly and efficiently determine the optimal time for harvesting. To this end, the characterization of variety-specific ripening curves and the development of new and rapid methods for determining grape ripeness are of key importance.