terclim by ICS banner
IVES 9 IVES Conference Series 9 EFFECT OF MANNOPROTEIN-RICH EXTRACTS FROM WINE LEES ON PHENOLICCOMPOSITION AND COLOUR OF RED WINE

EFFECT OF MANNOPROTEIN-RICH EXTRACTS FROM WINE LEES ON PHENOLICCOMPOSITION AND COLOUR OF RED WINE

Abstract

In 2022, wine production was estimated at around 260 million hl. This high production rate implies to generate a large amount of by-products, which include grape pomace, grape stalks and wine lees. It is estimated that processing 100 tons of grapes leads to ~ 22 tons of by-products from which ~ 6 tons are lees [1]. Wine lees are a sludge-looking material mostly made of dead and living yeast cells, yeast debris and other particles that precipitate at the bottom of wine tanks after alcoholic fermentation. Unlike grape pomace or grape stalks, few strategies have been proposed for the recovery and valorisation of wine less [2]. Nevertheless, this by-product could become a source of interesting compounds, such as mannoprotein rich extracts (MRE). Therefore, the aim of this work was to obtain MRE from different lees, to characterize them, and to evaluate their effect on wine colour and on the phenolic composition of red wines.

Red, rosé and white wines were used as sources of lees, which were collected after the alcoholic fermentation with different Saccharomyces cerevisiae commercial varieties. The extraction of MRE was performed by physical extraction (autoclave) followed by a purification with ethanol. The protein and polysaccharidic moieties of the purified extracts were characterized by SDS-PAGE, Lowry method, HR-SEC-RID and HPLC-DAD-MS. The obtained MRE were added to a red wine (Vitis vinifera L. cv Tempranillo) and the changes in the phenolic composition and colour were analysed by HPLC-DAD-MS and triestimulus colorimetry, respectively, before and after the stabilization of the wine (involving cold treatment). Results obtained showed that the extraction yield of MRE was efficient (~ 40 mg/g wet lees) for all types of lees assayed, which supports the valorisation of wine lees as a sustainable source of MRE. Interestingly, MRE presented important structural and compositional differences, both in the protein content and in the polysaccharidic profile, although the source of lees, namely red, white and rosé wines, was not the main factor determining these differences, but the winemaking techniques or the S. cerevisiae strain employed. Furthermore, the addition of the MRE to red wine had an effect on the stabilization of wine colour and its phenolic content that rely mainly on the saccharidic characteristics of each MRE. These results pointed out that MRE from wine less could be a potential tool to improve the colloidal stability of wine phenolic compounds.

 

1. Oliveira & Duarte, 2016. Front. Environ. Sci. Eng., 10(1): 168–176.
2. De Iseppi et al., 2020. Food Res. Int., 137, 109352. 

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Marcos, Martín-Andrés¹; Ignacio, García-Estévez¹; M. Teresa, Escribano-Bailón¹; Elvira Manjón¹

1. Department of Analytical Chemistry, Nutrition and Food Science, Universidad de Salamanca, Salamanca, E37007, Spain

Contact the author*

Keywords

lees, mannoprotein, colour wine, phenolic compounds

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

OPTIMIZING THE IDENTIFICATION OF NEW THIOLS AT TRACE LEVEL IN AGED RED WINES USING NEW OAK WOOD FUNCTIONALISATION STRATEGY

During bottle aging, many thiol compounds are involved in the expression of bouquet of great aged red wines according to the quality of the closure.1,2 Identifying thiol compounds in red wines is a challenging task due several drawbacks including, the complexity of the matrix, the low concentration of these impact compounds and the amount of wine needed.3,4
This work aims to develop a new strategy based on the functionalisation of oak wood organic extracts with H₂S, to produce new thiols, in order to mimic what can happen in red wine during bottle aging. Following this approach and through sensory analysis experiments, we demonstrated that the vanilla-like aroma of fresh oak wood was transformed into intense “meaty” nuances similar to those found in old but non oxidized red wines.

DO MICROPLASTICS IN VINEYARD SOIL AFFECT THE BIOAVAILABILITY OF VINE NUTRITION?

Microplastics can alter physicochemical and biogeochemical processes in the soil, but whether these changes have further effects on soil fertility, and if so, whether these effects vary depending on the type of soil in the vineyard and the type of plastic used in the vineyard. Knowing what types of plastics are currently used in vineyards in Slovenian viticultural regions as strings to tie vines to the stake, the aim of our study was to assess the effects of microplastic particles from polypropylene (PP) and polyvinyl chloride (PVC) on the availability of macro (potassium (K), Potassium (K), calcium (Ca), magnesium (Mg) and phosphate (P)) and micronutrients (iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn)) in two vineyard soils contrasting in pH and mineralogy. For this purpose, a short-term soil incubation experiment (120 days) was carried out in which the soil samples were enriched with micro-PP and micro-PVC particles. After the incubation period, macro- and micronutrient availability were measured.

EFFECTS OF BIODYNAMIC VINEYARD MANAGEMENT ON GRAPE RIPENING MECHANISMS

Biodynamic agriculture, founded in 1924 by Rudolph Steiner, is a form of organic agriculture. Through a holistic approach, biodynamic agriculture seeks to preserve the diversity of agriculture and the existing interactions between the mineral world and the different components of the organic world. Biodynamic grape production involves the use of composts, herbal teas and mineral preparations such as 500, 501 and CBMT.
Several scientific studies have provided evidence on the effects of biodynamic farming on the soil, the plant and the wine. Numerous empirical opinions of wine growers support the existence of differences brought by such a management.

EVOLUTION OF CHEMICAL AND SENSORIAL PROFILE OF WINES ELABORATED WITH THEIR OWN TOASTED VINE-SHOOTS AND MICRO-OXYGENATION

The positive contribution of toasted vine-shoots (SEGs, Shoot from vines – Enological – Granule) used in winemaking to the chemical and sensory profile of wines has been widely proven. However, the combination of this new enological tool with other winemaking technologies, such as micro-oxygenation (MOX), has not been studied so far. It is known that micro-oxygenation is used in wineries to stabilizes color, improves structure or combining with oak alternatives products to achieve a more effective aroma integration of wines. For that, its implementation in combination with SEGs could result in differentiated wines.

INSIGHT THE IMPACT OF GRAPE PRESSING ON MUST COMPOSITION

The pre-fermentative steps play a relevant role for the characteristics of white wine [1]. In particular, the grape pressing can affect the chemical composition and sensory profile and its optimized management leads to the desired extraction of aromas and their precursors, and phenols resulting in a balanced wine [2-4]. These aspects are important especially for must addressed to the sparkling wine as appropriate extraction of phenols is expected being dependent to grape composition, as well.