GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Georgian vitis germplasm: conservation, research and usage

Georgian vitis germplasm: conservation, research and usage

Abstract

Context and purpose of the study – Grapevine Vitis vinifera L. is a leader perennial crops for the Republic of Georgia, the South Caucasus. This is a region where the first wine making practice was initiated 8.000 years ago (McGovern et al. 2017) and a spot of grape domestication. The country of Georgia holds 525 local and more than 60 breeding varieties – they are preserved in 9 field collections inside the country.The list of recommended wine cultivars contains 34 names, including 27 old autochthonous varieties and covering 94% of the country’s vineyards. The wild grapevine Vitis silvestris Gmel. is a typical representative of the country’s flora. Importance of this genetic resources is essential for selection and breeding dew to new challenges such as climate change, diseases, environmental concerns and market demands. The present study was organized for multidisciplinary research of identification, collection, characterization and conservation for Georgian grapevine germplasm diversity including autochthonous varieties and wild grapevines with the aim to improve local viticulture and wine making.

Material and methods – The research fields of this study contains ampelography, ampelometry, phenology, cytology, ripening profiles, chemo-taxonomy, DNA fingerprinting and screening for disease. Modern techniques of ampelography based on the OIV descriptors, methodology of the European projects GrapeGen06 and COST FA1003, and molecular genetics (SSR, SNPs) were used to investigate Georgian autochthonous varieties from 5 collections (Georgia, Italy, France) and wild grape from the territory of Georgia. This research is in progress since 2003.

Results – This riches of genetic resources of Georgian grapes and assortment of its wines attracts the international interest because of its diversity. As a result several international (Bioversity International, GrapeGen06, COST FA1003) and national projects have run in the last decade, with Georgia as leader or regional coordinator – the latest being the ongoing “Research Project for the Study of Georgian Grapes and Wine Culture” (National Wine Agency) and the “Wild grapevine of Georgia: Research and Preservation” (Shota Rustaveli National Science Foundation). Being multidisciplinary these projects did grate effort for studding of wide aspects of Georgian grapes and wine, stimulated increasing of knowledge about them and promoting Georgian wines on the World market. As a result of these study large number of the local genetic resources have been certified using ampelography (morphology, phenology, anthocyanins), screening for resistances of downy mildew agent Plasmopara viticola, molecular fingerprinting, wine characteristics, made them available in the Vitis International Variety Catalogue and European Vitis database. The inventory of wild grape was carried out by organization of expeditions, more than 250 wild populations of Vitis silvestris Gmel.have been discovered and a field collection were established in 2014. Several books and articles dedicated to local varieties and wild grapevine were published in the last decade. But in the same time more efforts are needed to continue this work using new DNA technologies as well as ampelography technique in cooperation with other institutions.

DOI:

Publication date: March 11, 2024

Issue: GiESCO 2019

Type: Poster

Authors

David MAGHRADZE1*, Osvaldo FAILLA2, Roberto BACILIERI3, Gabriella DE LORENZIS 2Piero Attilio BIANCO2, Silvia TOFFOLATTI2, Rafael OCETE RUBIO4

1 Georgian Technical University (GTU), Tbilisi, Georgia
2 Department of Agricultural and Environmental Sciences, University of Milan, Italy
3 INRA, Montpellier SupAgro, Génétique de la Vigne, Montpellier, France
4 Laboratorio  de Entomologia Aplicada, Universidad de Sevilla, Spain

Contact the author

Keywords

Georgia, Grapevine, genetic resources, characterization, ampelography, DNA techniques

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

First step in the preparation of a soil map of the Protected Designation of Origin Valdepeñas (Central, Spain)

This work is a first step to make a map of vineyard soils. The characterization of the soils of the Protected Designation of Origin (D.P.O.) Valdepeñas will allow to group the studied profiles according to their physico-chemical characteristics and the concentrations of most relevant chemical elements. 90 soil profiles were analysed throughout the territory and the soils were sampled and described according to FAO (2006) and classified according to and Soil Taxonomy (2014). All samples were air dried, sieved and some physico-chemical parameters were determined following standard protocols. Also, major and trace elements were analysed by X-ray fluorescence. The statistically study was made using the SPSS program. Trend maps were made using the ArcGIS program. The studied soils have the following average properties: pH, 8.3; electrical conductivity, 0,20 dS/m (low); clay, 18.8% (medium) and CaCO3, 17.1% (high). In the study for the major elements. The major elements of these soils are Si, followed by Ca and Al, with an average content of 203.7 g/kg, 105.5 g/kg and 74.0 g/kg respectively. On the other hand, 27 trace elements have been studied. Of all of them, it can be highlighted the average values of Ba (361.8 mg/kg), Sr (129.3 mg/kg), Rb (83.4 mg/kg), V (74.2 mg/kg) and Ce (70.6 mg/kg). Ba, V and Ce values are higher and the values of Sr and Rb are lower to those found in the literature. The discriminant analysis shows a percentage of grouping of 91%. The content of chemical elements together with the physico-chemical characteristics allows grouping the soils in 4 group according to their order in the classification to Soil Taxonomy; due to the importance of the Calcisols in Castilla-La Mancha, it has been decided to establish them as their own group even if they do not appear in Soil Taxonomy classification.

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.

How does aromatic composition of red wines, resulting from varieties adapted to climate change, modulate fruity aroma?

One of the major issues for the wine sector is the impact of climate change linked to the increasing temperatures which affects physicochemical parameters of the grape varieties planted in Bordeaux vineyard and consequently, the quality of wine. In some varietals, the attenuation of their fresh fruity character is accompanied by the accentuation of dried-fruit notes [1]. As a new adaptive strategy on climate change, some winegrowers have initiated changes in the Bordeaux blend of vine varieties [2]. This study intends to explore the fruitiness in wines produced from grape varieties adapted to the future climate of Bordeaux. 10 commercial single–varietal wines from 2018 vintage made from the main grape varieties in the Bordeaux region (Cabernet franc, Cabernet-Sauvignon and Merlot) as well as from indigenous grape varieties from the Mediterranean basin, such as Cyprus (Yiannoudin), France (Syrah), Greece (Agiorgitiko and Xinomavro), Portugal (Touriga Nacional) and Spain (Garnacha and Tempranillo), were selected among 19 samples using sensory descriptive analyses. Both sensory and instrumental analyses were coupled, to investigate their fruity aroma expression. For sensory analysis, samples were prepared from wine, using a semi preparative HPLC method which preserves wine aroma and isolates fruity characteristics in 25 specific fractions [3,4]. Fractions of interest with intense fruity aromas were sensorially selected for each wine by a trained panel and mixed with ethanol and microfiltered water to obtain fruity aromatic reconstitutions (FAR) [5]. A free sorting task was applied to categorize FAR according to their similarities or dissimilarities, and different clusters were highlighted. Instrumental analysis of the different FAR and wines demonstrated variations in their molecular composition. Results obtained from sensory and gas chromatography analysis enrich the knowledge of the fruity expression of red wines from “new” grape varieties opening up new perspectives in wine technology, including blending, thus providing new tools for producers.